FastQCFastQC Report
Fri 17 Jun 2016
SRR1527433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3889
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACA3208.228336333247622No Hit
TGCGGAGGGCATGGGTTATATATATTATATATATGATACTGTATATTATTAT2085.348418616610954No Hit
TGCGGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1173.0084854718436618No Hit
TGCGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAC1142.9313448187194653No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1062.7256364103882746No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA782.005656981229108No Hit
TGCGGAGGGACTGCAAGGGACGTTTAATGTACTCTCATTTATGATGTCACCT751.9285163281049111No Hit
TGCGGAGGAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG641.6456672666495242No Hit
TGCGGAGGGTGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTTGC581.4913859604011315No Hit
TGCGGAGGGAGTGCTTGCGGAGGGGGGAATTCGGCCCGGAAACCCTGCGTCA521.3371046541527385No Hit
TGCGGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG521.3371046541527385No Hit
TGCGGAGGGGGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTTGG511.3113911031113397No Hit
TGCGGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAGACATTACAT441.131396245821548No Hit
TGCGGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT431.105682694780149No Hit
TGCGGAGGGGGAAATAATGCTGAAAAGAATTGCAGTCAAAAAAAAAAAAAAA431.105682694780149No Hit
TGCGGAGGGTCAACGCAGAGTGCAGTACTTGCGGAGGGTGCGGAGGGGGCGA391.002828490614554No Hit
TGCGGAGGGAGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAAAAAA370.9514013885317563No Hit
TGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACG330.848547184366161No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTG320.8228336333247621No Hit
TGCGGAGGGGGGCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.7714065312419645No Hit
TGCGGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT240.6171252249935717No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGCAAAAAAAAAAAAAA240.6171252249935717No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGCTAAAAAAAAAAAAA230.5914116739521729No Hit
TGCGGAGGGCAAGATAGATTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.565698122910774No Hit
TGCGGAGGGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.565698122910774No Hit
TGCGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.5142710208279764No Hit
TGCGGAGGGGCTAGGGATGTAATTTCATGTACTTTAATTCTTGCTAAGGAAG200.5142710208279764No Hit
TGCGGAGGGGGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTTGC170.4371303677037799No Hit
TGCGGAGGGATTATAGCACTATAAAAAGCTACTTTACCACCTGGCATACACC160.41141681666238106No Hit
TGCGGAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.41141681666238106No Hit
TGCGGAGGGGGCGATGAATGAACACTGCGGGCTTTGATGAAAAAAAAAAAAA140.35998971457958345No Hit
TGCGGAGGGGAGTGCAGTGCTTGCGGAGGGAAAAAAAAAAAAAAAAAAAAAA130.3342761635381846No Hit
TGCGGAGGGGAGCGGTGGCCGAGTGGTCGAAGGCGCTCGCCTGGAAAAAAAA130.3342761635381846No Hit
TGCGGAGGGGTGGTATCAATGCTTGCGGAGGGAAAAAAAAAAAAAAAAAAAA130.3342761635381846No Hit
TGCGGAGGGCAACATTACTAGACGTACCGAAAAAAAAAAAAAAAAAAAAAAA130.3342761635381846No Hit
TGCGGAGGGATATGCGCGAATTCCTGTAGAACGAACACCAAAAAAAAAAAAA130.3342761635381846No Hit
TGCGGAGGGGGGGTTCTGAGGTGACTGAGAAAAATGTTTCCTTTGTGTGGAA120.30856261249678585No Hit
TGCGGAGGGCAGAGTGCAGTGCTTGCGGAGGGGGTAAAAAAAAAAAAAAAAA120.30856261249678585No Hit
TGCGGAAGGCTGCTTCCCTCAAGAGTAACTATAACCTGCCCATGCACAAGAT120.30856261249678585No Hit
TGCGGAGGGCGCAGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAAA120.30856261249678585No Hit
TGCGGAGGGCTTGCGGAGGGGTGCAGTGCTTGCGGAGGGATAATACGCAAAA120.30856261249678585No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGAAAAAAAAAAAA120.30856261249678585No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.30856261249678585No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGATAAAAAAAAAAAAA110.282849061455387No Hit
TGCGGAGGGGTGTTGTTGTTGTGTTGTTGTTGTTGTTAAAAAAAAAAAAAAA110.282849061455387No Hit
TGCGGAGGGAAGCGTGGTATCCTTGCGGAGGGGCAAAAAAAAAAAAAAAAAA110.282849061455387No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTCAAAAAAAAAAAAAAAA100.2571355104139882No Hit
TGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTA100.2571355104139882No Hit
TGCGGAGGGAAGCAGTGGTATCATTGCGGAGGGGGGCAAAAAAAAAAAAAAA100.2571355104139882No Hit
TGCGGAGGGGGGTAGACCAAGATCCGAAAAAAAAAAAAAAAAAAAAAAAAAA100.2571355104139882No Hit
TGCGGAGGGAAGCAGTTGGTATCATACTTGCGGAGGGCAAAAAAAAAAAAAA100.2571355104139882No Hit
TGCGGAGGGGAAAAAAAAAGAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAA90.23142195937258936No Hit
TGCGGAGGGGGGTAGACCAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT90.23142195937258936Illumina Single End Adapter 2 (100% over 33bp)
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGCAAAAAAAAAAA90.23142195937258936No Hit
TGCGGAGGGGACATATCCCTCTTCCTGCAGAGTCTCCAGCAGTCGCCGCACA80.20570840833119053No Hit
TGCGGAAGGGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCCCAA80.20570840833119053Illumina Single End PCR Primer 1 (97% over 46bp)
TGCGGAGGGAAGCGTGGTATCAACGGCGGGGGGGAAAAAAAAAAAAAAAAAA80.20570840833119053No Hit
TGCGGAGGGACGCAGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAA80.20570840833119053No Hit
TGCGGAGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.20570840833119053No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTAAAAAAAAAAAAAA80.20570840833119053No Hit
TGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAACAA70.17999485728979173No Hit
TGCGGAGGGGAAAAAAAAAGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAA70.17999485728979173No Hit
TGCGGAGGGTGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTTTC70.17999485728979173No Hit
TGCGGAGGGAGTGCTTACGGAGGGCATTCACGCAGATCGAAAAAAAAAAAAA70.17999485728979173No Hit
TGCGGAGGGAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAA70.17999485728979173No Hit
TGCGGATGCGCAGTTCCCGCATGGGGTTCTCCTTTTCCCCTTGATCTTGCGC60.15428130624839292No Hit
TGCGGAGGGGTGGTATCAAGTGCAGTGCTTGCGGAGGGAAAAAAAAAAAAAA60.15428130624839292No Hit
TGCGGAGGGAGTGCAGTACTTGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAA60.15428130624839292No Hit
TGCGGAGGGCGCAGAGTGCATTGCTTGAGGGAAAAAAAAAAAAAAAAAAAAA60.15428130624839292No Hit
TGCGGAGGGGGGTAGACCAAGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA60.15428130624839292No Hit
TGCGGAGGGAGTGGTATCAACTGCGGAGGGGGGGGTAAAAAAAAAAAAAAAA60.15428130624839292No Hit
TGCGGAGGGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCGCA60.15428130624839292No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGAAAAAAAAAAAAAAA60.15428130624839292No Hit
TGCGGAGGGCGAGTGCAGTGCTTGCGGGGGAAAAAAAAAAAAAAAAAAAAAA60.15428130624839292No Hit
TGCGGAGGGTGTTGTTGTTGTTGTTGTTGTTGTTGTTGAAAAAAAAAAAAAA60.15428130624839292No Hit
TGCGGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.15428130624839292No Hit
TGCGGAGGGATGCGCGAATCCCTGTAGAACGAACACTAAAAAAAAAAAAAAA50.1285677552069941No Hit
TGCGGAGGCAGACTCGGCGGGGATATGACTGCAACGCAAAAAAAAAAAAAAA50.1285677552069941No Hit
TGCGGAGGGGAAAAAAAAAGAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAA50.1285677552069941No Hit
TGCGGAGGGAGAGTGCAGTGCTTGCGGGGGAAAAAAAAAAAAAAAAAAAAAA50.1285677552069941No Hit
TGCGGATGATCAGCCCATCCTTGATCAGCTTCCTGATCTGCTGACGGGAGTT50.1285677552069941No Hit
TGCGGAGGGAGTGCAGTGCTTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAA50.1285677552069941No Hit
TGCGGAGGGACCGCTTTTACGATTTCGTCAGTCTGCGCCTCGTTAGCCAGAG50.1285677552069941No Hit
TGCGGAGGGGCATGCGGAGGGGAGTACAGTGCTTGCGGAGGGGGAAAAAAAA50.1285677552069941No Hit
TGCGGAGGGGGGAAATGGAAGTCGCATGTTGGCAGGTGCTAAATAGAATTGC50.1285677552069941No Hit
TGCGGAGGGGCGTCTTTACGGTGCTTAAAACAAAAAAAAAAAAAAAAAAAAA50.1285677552069941No Hit
TGCGGACCCTAATGTGACGTTGGCGGATGAGCACACGGGCATGGTGAATAGA50.1285677552069941No Hit
TGCGGAGGGAGTGGTATCAATGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAA50.1285677552069941No Hit
TGCGGAGGGTAGCAGCACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1285677552069941No Hit
TGCGGAGGGGAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1285677552069941No Hit
TGCGGAGGGGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAAAAAAA40.10285420416559526No Hit
TGCGGAGGACCCTTGGAGGGGTTGGAGGGGGCTGGGAAGATGGGAGCAAACA40.10285420416559526No Hit
TGCGGACGGTTTCATAGTCGTACTCGAAGGGATTCTCTGTCCCCTTGGCACT40.10285420416559526No Hit
TGCGGAGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAAAAAAAAAA40.10285420416559526No Hit
TGCGGAGGGAGTGGTATCAACTGCGGAGGGGAAAAAAAAAAAAAAAAAAAAA40.10285420416559526No Hit
TGCGGAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA40.10285420416559526Illumina Single End Adapter 2 (97% over 34bp)
TGCGGAGGGAGTGGTATCAACGCTGCGGAGGGGAAAAAAAAAAAAAAAAAAA40.10285420416559526No Hit
TGCGGAGGGGGGGCAACTTGGCGTGCTGGCTGATTTCAATTTTCTTCACCAT40.10285420416559526No Hit
TGCGGAGGGAGACTCGGCGGTTATATGACTGCCTAACGCAAAAAAAAAAAAA40.10285420416559526No Hit
TGCGGAGGGGGGGGAGTGGAAGAGCGGCTGCCTGGAAGGCTTGGCTCGGGGC40.10285420416559526No Hit
TGCGGAGGGTGTGCAGTGCTTGCGGAGGGCAAAAAAAAAAAAAAAAAAAAAA40.10285420416559526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGA1050.046.0000045
AGGGGTT252.820785E-546.06
AGGGGGG451.72804E-1046.06
GGTTGTT252.820785E-546.09
AGGGGAC600.046.06
AGGGGAA451.72804E-1046.06
GGGTTGT252.820785E-546.08
CAGTCGC600.046.040
CAGCAGT600.046.037
CCAGCAG600.046.036
GTCGCCG600.046.042
CCTGCAG600.046.024
CTCTTCC600.046.019
CTCCAGC600.046.034
GGACATA600.046.09
CCGCACA600.046.046
TCCAGCA600.046.035
AGCAGTC600.046.038
GACATAT600.046.010
TTCCTGC600.046.022
Produced by FastQC (version 0.11.5)