FastQCFastQC Report
Fri 17 Jun 2016
SRR1527432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930186
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC81150.8724061639285047No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG57280.6157908203305575No Hit
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT43240.4648532659059586No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC36050.387556897222706No Hit
TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC28640.30789541016527877No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC28380.30510027026852693No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA26220.28187910804935784No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT25470.2738162045010353No Hit
TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC24820.2668283547591557No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG24450.2628506556753165No Hit
TGATCAGGGGAGCCAGGCTTGGTGGCACACGCCTTTAATCCCAGCACTTG23540.2530676660366851No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT21530.23145908452718056No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC21130.22715886930140855No Hit
TGATCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT20240.21759089042406574No Hit
TGATCAGGGGGGCAGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAG19780.2126456429144279No Hit
TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG16560.17802891034696286No Hit
TGATCAGGGGCTGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTTGGG16530.17770639420502998No Hit
TGATCAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA14970.16093555482451896No Hit
TGATCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC14360.1543777266052166No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT14310.15384019970199508No Hit
TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG13930.14975499523751165No Hit
TGATCAGGGGGGGCCGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACTT13410.14416471544400797No Hit
TGATCAGGGGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTGGAGAGG13320.14319716701820925No Hit
TGATCAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC13150.14136957554725615No Hit
TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC13110.14093955402467892No Hit
TGATCAGGGAGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGG12990.13964948945694733No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGA12940.13911196255372582No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT12700.13653183341826258No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC12490.13427422042473228No Hit
TGATCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC12480.13416671504408795No Hit
TGATCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA12380.13309166123764493No Hit
TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG12110.13018901596024882No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA11840.12728637068285267No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA11820.12707135992156407No Hit
TGATCAGGGGGCTGGAGAGATAGCTCAGTGATTAAGAGCATTGACTGCTC11630.12502875768932237No Hit
TGATCAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA11420.12277114469579202No Hit
TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG11180.1201910155603288No Hit
TGATCAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG11100.11933097251517438No Hit
TGATCAGGGTGCATGTATGTATGTATGAGTGTATGTGCATGAGTGTGTGT10980.11804090794744278No Hit
TGATCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG10790.11599830571520103No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC10750.11556828419262384No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT10570.11363318734102643No Hit
TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT10380.11159058510878471No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGTT10330.1110530582055632No Hit
TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA10280.1105155313023417No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTG10200.10965548825718728No Hit
TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC10100.10858043445074426No Hit
TGATCAGGGGGGCCGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACTTG10050.10804290754752276No Hit
TGATCAGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT9890.10632282145721395No Hit
TGATCAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC9760.10492525150883802No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT9620.10342017617981779No Hit
TGATCAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC9460.10170009008950898No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCT9420.10127006856693178No Hit
TGATCAGGGAGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCCCTGACTGC9390.10094755242499888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACG302.527193E-644.00000441
ACCGGTA302.527193E-644.00000431
ATAGGCG302.527193E-644.00000439
AAATAGG302.527193E-644.00000435
CGAAAGT302.527193E-644.00000415
ACGTTCC302.527193E-644.00000415
TAACGAC302.527193E-644.00000425
GTTAACG302.527193E-644.00000423
GTACTCG302.527193E-644.00000416
CTACCGA302.527193E-644.00000411
TTACGAA302.527193E-644.00000436
CCGGTTA600.044.00000440
CCGAATA302.527193E-644.00000436
ATTATGC302.527193E-644.00000432
GCGTATC302.527193E-644.00000438
ATACCGT302.527193E-644.00000443
TCGCCTA254.4421828E-544.028
TGATACG207.8557065E-444.027
CTGTACG254.4421828E-544.020
CGAGTAT254.4421828E-544.015
Produced by FastQC (version 0.11.5)