##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527432_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 930186 Sequences flagged as poor quality 0 Sequence length 50 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81620987630431 33.0 31.0 34.0 30.0 34.0 2 31.98327646298697 34.0 31.0 34.0 30.0 34.0 3 32.49542672110739 34.0 31.0 34.0 31.0 34.0 4 36.246342129423574 37.0 37.0 37.0 35.0 37.0 5 36.313748003087554 37.0 37.0 37.0 35.0 37.0 6 35.38265142670391 37.0 35.0 37.0 33.0 37.0 7 36.29544951224809 37.0 35.0 37.0 35.0 37.0 8 36.63359801158048 37.0 37.0 37.0 35.0 37.0 9 38.627455154130466 39.0 39.0 39.0 38.0 39.0 10 37.59753425658954 39.0 37.0 39.0 35.0 39.0 11 37.347202602490256 39.0 37.0 39.0 35.0 39.0 12 36.999366793308006 39.0 35.0 39.0 34.0 39.0 13 36.92746934484071 39.0 35.0 39.0 33.0 39.0 14 37.68467381792459 40.0 37.0 40.0 33.0 40.0 15 37.68908798885384 40.0 37.0 40.0 33.0 40.0 16 37.820979890043496 40.0 37.0 40.0 34.0 40.0 17 37.72732120242618 40.0 36.0 40.0 33.0 40.0 18 37.58024631632813 39.0 36.0 40.0 33.0 40.0 19 37.528744788676676 39.0 36.0 40.0 33.0 40.0 20 37.36542261440185 39.0 36.0 40.0 33.0 40.0 21 37.262005663383455 39.0 36.0 40.0 33.0 40.0 22 37.12898925591226 38.0 35.0 40.0 33.0 40.0 23 36.96679911329562 38.0 35.0 40.0 33.0 40.0 24 36.854882786883486 38.0 35.0 40.0 33.0 40.0 25 36.64586437551199 38.0 35.0 40.0 32.0 40.0 26 36.05900540322043 38.0 35.0 40.0 31.0 40.0 27 36.01083869247656 38.0 35.0 39.0 31.0 40.0 28 35.860129049458926 37.0 34.0 39.0 31.0 40.0 29 35.55144454979972 37.0 34.0 39.0 31.0 40.0 30 35.264189097664335 37.0 34.0 38.0 30.0 40.0 31 35.05084574482953 36.0 34.0 38.0 30.0 40.0 32 34.95367593148037 36.0 34.0 38.0 30.0 40.0 33 34.36234688546162 36.0 33.0 38.0 28.0 40.0 34 33.79227702846527 35.0 32.0 38.0 26.0 39.0 35 33.66715796625621 35.0 32.0 38.0 27.0 39.0 36 33.4771239300527 35.0 32.0 38.0 27.0 39.0 37 33.23898231106467 34.0 31.0 38.0 26.0 39.0 38 32.80955851840385 34.0 31.0 37.0 26.0 38.0 39 32.04379124175165 34.0 30.0 37.0 24.0 38.0 40 31.801204275273978 34.0 30.0 36.0 23.0 38.0 41 31.409212781099694 33.0 29.0 36.0 23.0 38.0 42 31.06724139043159 33.0 29.0 35.0 23.0 38.0 43 30.64007198560288 32.0 28.0 35.0 22.0 38.0 44 30.18800433461695 32.0 27.0 35.0 21.0 37.0 45 29.75847948689832 31.0 27.0 34.0 20.0 37.0 46 29.648724018637132 31.0 27.0 34.0 20.0 37.0 47 29.180527335393137 31.0 26.0 34.0 20.0 37.0 48 28.6797704975134 31.0 26.0 34.0 19.0 36.0 49 27.935286061067355 30.0 24.0 33.0 18.0 35.0 50 31.11143792746827 33.0 29.0 35.0 23.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 3.0 13 7.0 14 17.0 15 33.0 16 78.0 17 170.0 18 259.0 19 467.0 20 751.0 21 1172.0 22 1772.0 23 2582.0 24 3773.0 25 5348.0 26 7185.0 27 10076.0 28 13995.0 29 19023.0 30 26333.0 31 36927.0 32 57936.0 33 95036.0 34 115723.0 35 193131.0 36 261333.0 37 74932.0 38 2121.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.5568628209842 0.32423622802321256 0.10460273536690512 0.014298215625692064 8 98.82270857656425 0.9929196956307663 0.10320516541852921 0.08116656238644744 9 97.79592468603053 1.3260788702474557 0.39637233843553865 0.4816241052864696 10 64.76392893464318 25.37739763875182 3.328259079366922 6.53041434723808 11 40.03253112818296 25.801936386916164 16.379949816488313 17.78558266841255 12 35.31143233718848 17.193228021062453 24.240205722296402 23.25513391945267 13 26.739920833037694 16.94263297878059 26.238300726951387 30.079145461230333 14 26.946868690777976 17.593900574723765 29.720077489878367 25.739153244619896 15 30.143863700378205 17.788915335212526 27.635978180707944 24.43124278370132 16 35.51838019492875 18.44039794191699 23.456168981257512 22.58505288189674 17 34.26906016646133 20.22337467990273 21.084815295005516 24.422749858630425 18 31.827720477409894 21.071592133186265 24.32395241381831 22.776734975585526 19 33.15992715435407 21.446033373970366 21.58138264820154 23.812656823474015 20 32.33718847628324 22.480450146529833 22.377029970350016 22.805331406836913 21 33.03317831057445 21.227797451262436 24.504561453300738 21.23446278486238 22 30.44047104557583 21.90465132779896 25.518767214299075 22.136110412326136 23 34.15768459211384 20.926889890839036 23.4668120139413 21.448613503105832 24 34.58243835103947 19.784322705351403 21.061379122025055 24.57185982158407 25 30.43144059360171 19.69724334702952 21.531500151582588 28.339815907786186 26 27.526430197831402 19.06898190254422 23.312864308858657 30.091723590765717 27 26.609301795554867 19.570709514011174 23.176332475440393 30.64365621499356 28 28.339815907786186 23.799433661654763 20.132747644019584 27.72800278653947 29 31.746123893500872 22.775337405637153 20.587387898764334 24.891150802097645 30 31.505526851618924 20.55610383299684 22.680410154528236 25.257959160856004 31 33.349566645810626 20.6965058601183 20.64329069669937 25.31063679737171 32 35.64760166246321 20.7030636883376 20.693710720221546 22.955623928977644 33 33.87086023655484 20.970107053858044 23.01797704975134 22.141055659835775 34 28.800261453085728 22.752976286463138 27.78089543381646 20.665866826634673 35 26.939450819513517 28.295953712483307 25.39642609112586 19.36816937687731 36 27.499338841909037 30.518627457304238 20.919041998052002 21.06299170273472 37 29.842741129193517 29.092891099199512 19.35064599983229 21.713721771774676 38 31.74494133431378 28.70081897598975 19.20261109068509 20.35162859901138 39 31.290623595710965 25.519304741202298 19.23937793086544 23.9506937322213 40 29.488080878447963 24.164091912800238 19.911609076034257 26.436218132717542 41 24.948666180742347 25.942338414037625 20.949573526154985 28.15942187906505 42 25.502748912583073 24.45188381678503 20.73348771105994 29.311879559571956 43 25.207109115811242 22.055373871462265 22.87004964598478 29.867467366741707 44 27.06770473862217 21.760916633877525 23.831362759706124 27.340015867794182 45 27.995153657440554 22.722982285263377 21.699423556148986 27.582440501147083 46 27.368827309806854 25.845798582219043 20.887865437665155 25.897508670308948 47 25.757859180851998 23.50282631645714 24.51509698060388 26.224217522086978 48 29.423792660822674 22.031292666197942 24.040030703536715 24.50488396944267 49 28.84326360534345 22.23523037328018 24.084107909600874 24.837398111775492 50 26.824097546082182 21.843158250070417 23.60581647111438 27.726927732733024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.5 4 3.0 5 13.0 6 23.0 7 64.5 8 106.0 9 324.5 10 543.0 11 742.5 12 942.0 13 1083.5 14 1225.0 15 1134.5 16 1044.0 17 1125.5 18 1207.0 19 1397.0 20 1587.0 21 1684.0 22 1781.0 23 2118.0 24 2455.0 25 3059.5 26 3664.0 27 4409.0 28 5154.0 29 6261.0 30 7368.0 31 8602.0 32 9836.0 33 11088.0 34 12340.0 35 12709.5 36 13079.0 37 14235.5 38 15392.0 39 17118.5 40 18845.0 41 23563.5 42 28282.0 43 28217.5 44 28153.0 45 26585.5 46 25018.0 47 28626.0 48 32234.0 49 36418.0 50 40602.0 51 43540.5 52 46479.0 53 54166.5 54 61854.0 55 72475.5 56 83097.0 57 90005.5 58 96914.0 59 96041.0 60 95168.0 61 94288.0 62 93408.0 63 82345.5 64 71283.0 65 59732.0 66 48181.0 67 38152.5 68 28124.0 69 23883.5 70 19643.0 71 15802.5 72 11962.0 73 13836.0 74 15710.0 75 10476.0 76 5242.0 77 3201.5 78 1161.0 79 1028.0 80 895.0 81 497.5 82 100.0 83 81.5 84 63.0 85 40.0 86 17.0 87 9.0 88 1.0 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 930186.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.108954432171046 #Duplication Level Percentage of deduplicated Percentage of total 1 72.36854183840684 8.763073754439322 2 9.611981333781467 2.327820879472769 3 3.666859347029687 1.3320549822708885 4 1.7151970700620713 0.8307697265429964 5 1.0253218188673678 0.6207787591487846 6 0.6918290127489455 0.5026395594138516 7 0.47696566277565894 0.4042888833382501 8 0.389595762029199 0.377407786350282 9 0.34412800089084056 0.3750327253459175 >10 6.028480488794725 19.785728466176057 >50 2.3045877047771994 19.236673662822838 >100 1.2668545951599226 30.460450620258676 >500 0.0668642467536905 5.406883549872788 >1k 0.04101007134226351 8.081987360711317 >5k 0.0017830465800984133 1.4944092838352603 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 8115 0.8724061639285047 No Hit TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 5728 0.6157908203305575 No Hit TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 4324 0.4648532659059586 No Hit TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 3605 0.387556897222706 No Hit TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 2864 0.30789541016527877 No Hit TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 2838 0.30510027026852693 No Hit TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA 2622 0.28187910804935784 No Hit TGATCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT 2547 0.2738162045010353 No Hit TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC 2482 0.2668283547591557 No Hit TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG 2445 0.2628506556753165 No Hit TGATCAGGGGAGCCAGGCTTGGTGGCACACGCCTTTAATCCCAGCACTTG 2354 0.2530676660366851 No Hit TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 2153 0.23145908452718056 No Hit TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC 2113 0.22715886930140855 No Hit TGATCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 2024 0.21759089042406574 No Hit TGATCAGGGGGGCAGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAG 1978 0.2126456429144279 No Hit TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG 1656 0.17802891034696286 No Hit TGATCAGGGGCTGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTTGGG 1653 0.17770639420502998 No Hit TGATCAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA 1497 0.16093555482451896 No Hit TGATCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC 1436 0.1543777266052166 No Hit TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT 1431 0.15384019970199508 No Hit TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG 1393 0.14975499523751165 No Hit TGATCAGGGGGGGCCGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACTT 1341 0.14416471544400797 No Hit TGATCAGGGGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTGGAGAGG 1332 0.14319716701820925 No Hit TGATCAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC 1315 0.14136957554725615 No Hit TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC 1311 0.14093955402467892 No Hit TGATCAGGGAGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGG 1299 0.13964948945694733 No Hit TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGA 1294 0.13911196255372582 No Hit TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT 1270 0.13653183341826258 No Hit TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC 1249 0.13427422042473228 No Hit TGATCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC 1248 0.13416671504408795 No Hit TGATCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA 1238 0.13309166123764493 No Hit TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG 1211 0.13018901596024882 No Hit TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA 1184 0.12728637068285267 No Hit TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 1182 0.12707135992156407 No Hit TGATCAGGGGGCTGGAGAGATAGCTCAGTGATTAAGAGCATTGACTGCTC 1163 0.12502875768932237 No Hit TGATCAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA 1142 0.12277114469579202 No Hit TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 1118 0.1201910155603288 No Hit TGATCAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG 1110 0.11933097251517438 No Hit TGATCAGGGTGCATGTATGTATGTATGAGTGTATGTGCATGAGTGTGTGT 1098 0.11804090794744278 No Hit TGATCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 1079 0.11599830571520103 No Hit TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC 1075 0.11556828419262384 No Hit TGATCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT 1057 0.11363318734102643 No Hit TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT 1038 0.11159058510878471 No Hit TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGTT 1033 0.1110530582055632 No Hit TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA 1028 0.1105155313023417 No Hit TGATCAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTG 1020 0.10965548825718728 No Hit TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 1010 0.10858043445074426 No Hit TGATCAGGGGGGCCGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACTTG 1005 0.10804290754752276 No Hit TGATCAGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT 989 0.10632282145721395 No Hit TGATCAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC 976 0.10492525150883802 No Hit TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT 962 0.10342017617981779 No Hit TGATCAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC 946 0.10170009008950898 No Hit TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCT 942 0.10127006856693178 No Hit TGATCAGGGAGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCCCTGACTGC 939 0.10094755242499888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.07245862655425904 0.0 0.0 0.0 0.0 7 0.07955398167678292 0.0 0.0 0.0 0.0 8 0.08030651934129303 0.0 0.0 0.0 0.0 9 0.08191910005095755 0.0 0.0 0.0 0.0 10 0.09664733719922682 0.0 0.0 0.0 0.0 11 0.13416671504408795 0.0 0.0 0.0 0.0 12 0.15136757594717615 0.0 0.0 0.0 0.0 13 0.16211811401160628 0.0 0.0 0.0 0.0 14 0.1807165448630704 0.0 0.0 0.0 0.0 15 0.19512226586940676 0.0 0.0 0.0 1.0750538064430124E-4 16 0.21296815905636077 0.0 0.0 0.0 1.0750538064430124E-4 17 0.22619132087560984 0.0 0.0 0.0 1.0750538064430124E-4 18 0.23737188046261715 0.0 0.0 0.0 1.0750538064430124E-4 19 0.2532826767979737 0.0 0.0 0.0 1.0750538064430124E-4 20 0.2684409354688202 0.0 0.0 0.0 1.0750538064430124E-4 21 0.27940648429453896 0.0 0.0 0.0 1.0750538064430124E-4 22 0.29456474296538543 0.0 0.0 0.0 1.0750538064430124E-4 23 0.3084329370685003 0.0 0.0 0.0 1.0750538064430124E-4 24 0.3260638194941657 0.0 0.0 0.0 1.0750538064430124E-4 25 0.3449847664875627 0.0 0.0 0.0 1.0750538064430124E-4 26 0.36369070271967113 0.0 0.0 0.0 1.0750538064430124E-4 27 0.3850842734678871 0.0 0.0 0.0 1.0750538064430124E-4 28 0.4147557585257142 0.0 0.0 0.0 1.0750538064430124E-4 29 0.43410672704168846 0.0 0.0 0.0 1.0750538064430124E-4 30 0.4565753515963474 0.0 0.0 0.0 1.0750538064430124E-4 31 0.47463625554459 0.0 0.0 0.0 1.0750538064430124E-4 32 0.5006525576605109 0.0 0.0 0.0 1.0750538064430124E-4 33 0.5151657840474916 0.0 0.0 0.0 1.0750538064430124E-4 34 0.533549204137667 0.0 0.0 0.0 1.0750538064430124E-4 35 0.5658008183309574 0.0 0.0 0.0 1.0750538064430124E-4 36 0.5983749486661808 0.0 0.0 0.0 1.0750538064430124E-4 37 0.624498756162746 0.0 0.0 0.0 1.0750538064430124E-4 38 0.6449247784851632 0.0 0.0 0.0 1.0750538064430124E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCACG 30 2.527193E-6 44.000004 41 ACCGGTA 30 2.527193E-6 44.000004 31 ATAGGCG 30 2.527193E-6 44.000004 39 AAATAGG 30 2.527193E-6 44.000004 35 CGAAAGT 30 2.527193E-6 44.000004 15 ACGTTCC 30 2.527193E-6 44.000004 15 TAACGAC 30 2.527193E-6 44.000004 25 GTTAACG 30 2.527193E-6 44.000004 23 GTACTCG 30 2.527193E-6 44.000004 16 CTACCGA 30 2.527193E-6 44.000004 11 TTACGAA 30 2.527193E-6 44.000004 36 CCGGTTA 60 0.0 44.000004 40 CCGAATA 30 2.527193E-6 44.000004 36 ATTATGC 30 2.527193E-6 44.000004 32 GCGTATC 30 2.527193E-6 44.000004 38 ATACCGT 30 2.527193E-6 44.000004 43 TCGCCTA 25 4.4421828E-5 44.0 28 TGATACG 20 7.8557065E-4 44.0 27 CTGTACG 25 4.4421828E-5 44.0 20 CGAGTAT 25 4.4421828E-5 44.0 15 >>END_MODULE