FastQCFastQC Report
Fri 17 Jun 2016
SRR1527431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712164
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC62450.8769047578928448No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT46400.6515353205160609No Hit
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG35160.4937065058048427No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT26890.37758156829045No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA23990.3368606107581961No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG22610.3174830516566409No Hit
TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC22260.3125684533337827No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT20590.289118798479002No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT20580.28897838138406323No Hit
TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT19470.2733920838458557No Hit
TGATCAGGGGAGCCAGGCTTGGTGGCACACGCCTTTAATCCCAGCACTTGGG18500.2597716256367915No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16450.23098612117433623No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC16310.229020281845193No Hit
TGATCAGGGGGGCAGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAGGC16310.229020281845193No Hit
TGATCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT15620.21933150229441534No Hit
TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14000.19658393291432874No Hit
TGATCAGGGGCTGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTTGGGAG13570.19054599783196005No Hit
TGATCAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG12230.17173010711016No Hit
TGATCAGGGGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTGGAGAGGCA11130.15628422666689135No Hit
TGATCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT10920.1533354676731764No Hit
TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT10780.1513696283440331No Hit
TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG10380.14575294454648086No Hit
TGATCAGGGGGGGCCGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACTTGG10240.14378710521733756No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC10140.1423829342679495No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT10120.1421021000780719No Hit
TGATCAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC10050.14111918041350024No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC9590.13465999404631518No Hit
TGATCAGGGAGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGA9580.13451957695137637No Hit
TGATCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG9550.13409832566655996No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC9120.12806039058419127No Hit
TGATCAGGGGGCTGGAGAGATAGCTCAGTGATTAAGAGCATTGACTGCTCTT9000.1263753854449256No Hit
TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8890.12483079740059873No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC8730.12258412388157784No Hit
TGATCAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG8710.12230328969170022No Hit
TGATCAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT8630.12117995293218976No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC8620.12103953583725098No Hit
TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG8510.11949494779292409No Hit
TGATCAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA8420.11823119393847485No Hit
TGATCAGGGGGGCCGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACTTGGG8240.11570368622957634No Hit
TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC8230.11556326913463753No Hit
TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC8140.11429951528018827No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT8100.11373784690043305No Hit
TGATCAGGGTGCATGTATGTATGTATGAGTGTATGTGCATGAGTGTGTGTAT7950.11163159047635095No Hit
TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA7860.1103678366219017No Hit
TGATCAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG7820.10980616824214648No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT7770.10910408276745244No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG7710.10826158019781959No Hit
TGATCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA7700.1081211631028808No Hit
TGATCAGGGAAGAGCACACTGAGGAGGCCAGCCTTAAAGGCTCTCAGGAGGC7590.10657657505855392No Hit
TGATCAGGGGCTGCCGCCGCCCTGAGTCACTGCCTGCGCAGCTCCCGCCGCC7520.10559365539398227No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGTTCT7480.10503198701422706No Hit
TGATCAGGGAGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCCCTGACTGCTC7350.10320656478002257No Hit
TGATCAGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT7320.10278531349520617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGG206.3098286E-446.017
AGACTTA253.415837E-546.031
TAATACC206.3098286E-446.015
GATACGC206.3098286E-446.028
CAACGTG206.3098286E-446.022
CGAGTAT301.8603987E-646.015
AAATAGG206.3098286E-446.035
TTTACGA405.6061253E-946.035
CAACGAA206.3098286E-446.041
TCCGAAT453.092282E-1046.021
ACCGCGA206.3098286E-446.033
CTATATG253.415837E-546.013
CCAATCG206.3098286E-446.012
GAATCGA206.3098286E-446.021
TATATGC301.8603987E-646.037
CGTCTAG206.3098286E-446.010
TTCCGAA206.3098286E-446.039
TAACGCT206.3098286E-446.045
TATGCGC301.8603987E-646.015
GCGCATA453.092282E-1046.025
Produced by FastQC (version 0.11.5)