FastQCFastQC Report
Fri 17 Jun 2016
SRR1527430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994096
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC88040.8856287521527096No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT60740.6110073876164878No Hit
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG47900.4818448117686823No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT39100.3933221741159808No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA32210.32401297259017237No Hit
TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC31730.3191844650818432No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG29290.2946395519145032No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT27690.2785445268867393No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT26720.2687869179636574No Hit
TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT26000.2615441567011637No Hit
TGATCAGGGGAGCCAGGCTTGGTGGCACACGCCTTTAATCCCAGCACTTGGG25730.2588281212277285No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC22850.22985707617775344No Hit
TGATCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT22340.2247267869501537No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC22310.22442500523088316No Hit
TGATCAGGGGGGCAGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAGGC21610.2173834317812364No Hit
TGATCAGGGGCTGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTTGGGAG18290.1839862548486263No Hit
TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG18190.18298031578439106No Hit
TGATCAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG16990.17090904701356813No Hit
TGATCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT16340.16437044309603902No Hit
TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT15470.1556187732371924No Hit
TGATCAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC14890.14978432666462796No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC14610.1469676972847693No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT14600.14686710337834574No Hit
TGATCAGGGGGGGCCGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACTTGG14580.1466659155654987No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG14250.1433463166535224No Hit
TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG14130.1421391897764401No Hit
TGATCAGGGGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTGGAGAGGCA14070.14153562633789896No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC13170.13248217475978175No Hit
TGATCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG13100.1317780174148171No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC13070.1314762356955465No Hit
TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG12990.13067148444415833No Hit
TGATCAGGGAGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGA12690.12765366725145258No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC12630.12705010381291143No Hit
TGATCAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA12330.1240322866202057No Hit
TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG12210.12282515974312339No Hit
TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC11990.12061209380180586No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT11910.11980734255041767No Hit
TGATCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA11910.11980734255041767No Hit
TGATCAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG11900.11970674864399415No Hit
TGATCAGGGGGCTGGAGAGATAGCTCAGTGATTAAGAGCATTGACTGCTCTT11880.11950556083114708No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT11810.11880140348618241No Hit
TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC11790.11860021567333537No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC11710.11779546442194717No Hit
TGATCAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT11690.11759427660910013No Hit
TGATCAGGGGGGCCGGGCGTGGTGGTGCACGCCTTTAATCCCAGCACTTGGG11620.11689011926413546No Hit
TGATCAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG10960.11025092144018284No Hit
TGATCAGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT10790.10854082503098292No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGTTCT10740.1080378554988653No Hit
TGATCAGGGGCTGCCGCCGCCCTGAGTCACTGCCTGCGCAGCTCCCGCCGCC10380.10441647486761842No Hit
TGATCAGGGAGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCCCTGACTGCTC10280.10341053580338318No Hit
TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA10170.1023040028327244No Hit
TGATCAGGGAAGAGCACACTGAGGAGGCCAGCCTTAAAGGCTCTCAGGAGGC10110.10170043939418327No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCTCT10050.10109687595564211No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT10030.10089568814279506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAT351.01937985E-746.00000421
GCGCATA351.01937985E-746.00000425
TTATCGT351.01937985E-746.00000441
CAATCGA700.046.00000443
TATCGTA351.01937985E-746.00000442
CACGCTA206.311041E-446.042
ATAGCCG551.8189894E-1246.023
GTTAGTC206.311041E-446.012
GTTAGTA206.311041E-446.016
CGTCTAG301.8611463E-646.010
CACGATT206.311041E-446.014
CGATTCA206.311041E-446.016
ACGATTC206.311041E-446.015
ATATCCC206.311041E-446.045
GTTAACG301.8611463E-646.023
CTACGTC206.311041E-446.021
TGCGATT600.046.039
AACGCGT206.311041E-446.020
TTGCGAA206.311041E-446.013
CGAATCA253.416821E-546.010
Produced by FastQC (version 0.11.5)