SRR1527429_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49970
Sequences flagged as poor quality	0
Sequence length	50
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGA	2056	4.114468681208725	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	725	1.450870522313388	Illumina Single End Adapter 2 (100% over 23bp)
GCGTCCGGGGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTT	460	0.9205523313988393	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG	406	0.8124874924954972	No Hit
GCGTCCTTATGAATGTTTTGACCATTTTCATGGTGGAAGAATTTTATATT	381	0.7624574744846908	No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAA	348	0.6964178507104263	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGCGA	238	0.4762857714628777	No Hit
GCGTCCGGGGAGGAAGGGCGTAGTAAGCGACGAAATGCTTCGGGGAGTTG	226	0.4522713628176906	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC	221	0.4422653592155293	Illumina Single End Adapter 2 (95% over 22bp)
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAA	220	0.4402641584950971	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCAGAGCTCG	213	0.42625575345207123	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGCAGAGCTCG	212	0.424254552731639	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTGCGGGA	193	0.38623173904342606	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGGGA	184	0.3682209325595357	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC	179	0.3582149289573744	Illumina Single End Adapter 2 (100% over 21bp)
GCGTCCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTT	169	0.33820292175305183	Illumina Single End Adapter 2 (100% over 27bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGCAGTGCCGCGTATGCCG	168	0.3362017210326196	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGCGA	161	0.3221933159895937	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGC	156	0.31218731238743247	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGG	156	0.31218731238743247	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGCAGTGCCTCGTATGCCG	151	0.30218130878527116	No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGAAG	144	0.28817290374224536	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTC	144	0.28817290374224536	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCGTATGGC	128	0.2561536922153292	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGAGA	127	0.25415249149489694	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGAAGAGCTCG	122	0.24414648789273563	No Hit
GCGTCCGGGGTTGGACGCGTCCGGTGCCGGGGGTGCTGTTCCCTCGATCC	122	0.24414648789273563	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGA	119	0.23814288573143885	No Hit
GCGTCCGGGGGAAACAAAGGAAAGCAGGAGGGAATTTCCTAAGATTGGAT	119	0.23814288573143885	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGATCGGAAGAG	118	0.2361416850110066	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTACGGGA	116	0.23213928357014207	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGAAGAGCTCG	114	0.22813688212927757	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA	112	0.22413448068841305	No Hit
GCGTCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA	111	0.2221332799679808	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGCGTATGCCG	108	0.21612967780668402	No Hit
GCGTCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA	101	0.2021212727636582	Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCAGAGCTCGTAT	100	0.20012007204322593	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGAGCTCGTATGCCG	99	0.19811887132279368	Illumina Single End Adapter 2 (95% over 23bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTAT	96	0.1921152691614969	No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGCAGAGTGCAGTGCTGCGTCCG	90	0.18010806483890335	No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	90	0.18010806483890335	No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86	0.1721032619571743	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATG	84	0.1681008605163098	No Hit
GCGTCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	83	0.1660996597958775	Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGAGCTCGT	81	0.162097258355013	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGATCGCGA	80	0.16009605763458074	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCTGGA	80	0.16009605763458074	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGCAGTGGCTCGTATGCCG	79	0.15809485691414848	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAATAGCTCGTATGCCG	79	0.15809485691414848	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGT	78	0.15609365619371623	Illumina Single End Adapter 2 (95% over 24bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGCAGTGCCGCGTACGCCG	77	0.15409245547328396	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGT	75	0.15009005403241946	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGCGTACGCCG	73	0.14608765259155493	No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAAA	73	0.14608765259155493	No Hit
GCGTCCGGGGAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGA	71	0.1420852511506904	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGGA	70	0.14008405043025815	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGAGAGCTCGT	69	0.1380828497098259	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTT	67	0.13408044826896137	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAAGAGCTCG	66	0.13207924754852912	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGCCCGGGA	65	0.13007804682809684	No Hit
GCGTCCGGGGAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGG	65	0.13007804682809684	No Hit
GCGTCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	65	0.13007804682809684	Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTGCCGGC	64	0.1280768461076646	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGATGCCGT	64	0.1280768461076646	No Hit
GCGTCCGGGAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAA	64	0.1280768461076646	No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAA	63	0.12607564538723234	No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAA	60	0.12007204322593557	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCG	60	0.12007204322593557	No Hit
GCGTCCGGGTTAATCTACACACTCAACAGTTCCAGTCAAAATTCAAACAG	60	0.12007204322593557	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGCGT	59	0.1180708425055033	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGAGCAGTGCAGCGCGTATGC	59	0.1180708425055033	No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAA	59	0.1180708425055033	No Hit
GCGTCCGGGCAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAA	59	0.1180708425055033	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGAACGGGA	58	0.11606964178507104	No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57	0.11406844106463879	No Hit
GCGTCCGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	57	0.11406844106463879	Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTTCGGGA	56	0.11206724034420652	No Hit
GCGTCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAC	56	0.11206724034420652	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCAGAAGAGCTCGTATGC	54	0.10806483890334201	Illumina Single End Adapter 2 (95% over 21bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGCAATAGCTCGTATGCCG	54	0.10806483890334201	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCGTATGCC	54	0.10806483890334201	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTCGGAAGAGCTCGT	54	0.10806483890334201	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCGTCTGGC	53	0.10606363818290974	No Hit
GCGTCCGGGAAGCAGTGGTATCAGATCGGAAGAGCTCGTATGCCGTCTTC	53	0.10606363818290974	Illumina Single End Adapter 2 (100% over 28bp)
GCGTCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	53	0.10606363818290974	Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGATCACAGAAGGGCGGCAGCATCTCTGGGCTTTGCTTCTGTA	52	0.1040624374624775	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGAGCAGTAGAGCTCGTATGC	51	0.10206123674204523	No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCTCGTATGCCG	51	0.10206123674204523	No Hit
GCGTCCGGGGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	50	0.10006003602161297	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAT	20	7.784292E-4	44.000004	23
TGTGAGT	20	7.784292E-4	44.000004	43
GAGGCCG	20	7.784292E-4	44.000004	41
CAATGTG	20	7.784292E-4	44.000004	40
GGGTCTC	20	7.784292E-4	44.000004	8
TTCGTTT	20	7.784292E-4	44.000004	21
TGCGTTC	20	7.784292E-4	44.000004	40
ATAAGGT	20	7.784292E-4	44.000004	15
TGCGTGA	20	7.784292E-4	44.000004	44
ACCATTT	20	7.784292E-4	44.000004	21
GGCAATG	20	7.784292E-4	44.000004	38
ACGCAGG	40	8.041752E-9	44.000004	24
GGGTAAG	40	8.041752E-9	44.000004	7
GAAGCGT	20	7.784292E-4	44.000004	9
TTTCATG	20	7.784292E-4	44.000004	26
TTTTCAT	20	7.784292E-4	44.000004	25
GGTTCGT	20	7.784292E-4	44.000004	19
TTATAGA	20	7.784292E-4	44.000004	26
GTGAGTT	20	7.784292E-4	44.000004	44
GCAATGT	20	7.784292E-4	44.000004	39

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content