FastQCFastQC Report
Fri 17 Jun 2016
SRR1527428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39795
Sequences flagged as poor quality0
Sequence length52
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGAAG15663.93516773464003No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC5301.3318256062319387Illumina Single End Adapter 2 (100% over 25bp)
GCGTCCGGGGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTAC4501.1307953260459858No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA3440.864430204799598No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGGAG3140.7890438497298655No Hit
GCGTCCTTATGAATGTTTTGACCATTTTCATGGTGGAAGAATTTTATATTTA3130.7865309712275411No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAA3030.761402186204297No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGAAG2630.6608870461113205No Hit
GCGTCCGGGGAGGAAGGGCGTAGTAAGCGACGAAATGCTTCGGGGAGTTGAA2060.517652971478829No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG1990.500062821962558Illumina Single End Adapter 2 (100% over 23bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT1970.4950370649579093Illumina Single End Adapter 2 (95% over 24bp)
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAA1970.4950370649579093No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAGGGGCTCGTATGCCGTC1790.44980525191606985No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGGGAAG1630.4095991958788793No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGGAG1400.3518029903254178No Hit
GCGTCCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT1350.3392385978137957Illumina Single End Adapter 2 (100% over 29bp)
GCGTCCGGGTTAATCTACACACTCAACAGTTCCAGTCAAAATTCAAACAGGG1220.3065711772835783No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGAAGCA1190.2990325417766051No Hit
GCGTCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA1160.2914939062696319Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGCCCGGGAAG1080.2713908782510366No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG1080.2713908782510366No Hit
GCGTCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1050.2638522427440633Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGCAGAGTGCAGTGCTGCGTCCGGG960.2412363362231436No Hit
GCGTCCGGGGGAAACAAAGGAAAGCAGGAGGGAATTTCCTAAGATTGGATAC940.2362105792184948No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGGAAG920.23118482221384595No Hit
GCGTCCGGGGTTGGACGCGTCCGGTGCCGGGGGTGCTGTTCCCTCGATCCCT900.22615906520919715No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCCGGGAGCGGGCGG900.22615906520919715No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT870.2186204297022239No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTCGGATGCCGTCT840.21108179419525064No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGACG820.20605603719060184No Hit
GCGTCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA820.20605603719060184No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGC800.201030280185953Illumina Single End Adapter 2 (95% over 21bp)
GCGTCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA790.1985174016836286Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAA770.19349164467897978No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT700.1759014951627089Illumina Single End Adapter 2 (96% over 26bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGCGC700.1759014951627089No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.17338861666038446No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA680.17087573815806006No Hit
GCGTCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA670.16836285965573564Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGCGTCCGGGGAG660.16584998115341124No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTCCGGGAAG660.16584998115341124No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1633371026510868No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAAAAA640.1608242241487624No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTT630.15831134564643798No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCC630.15831134564643798Illumina Single End Adapter 2 (95% over 22bp)
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAAAA610.15328558864178915No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTAT610.15328558864178915No Hit
GCGTCCGGGAAGCAGTGGTATCAACGGATCGGAAGAGCTCGTATGCCGTCTT600.15077271013946475Illumina Single End Adapter 2 (96% over 27bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGCCCGGGTCG600.15077271013946475No Hit
GCGTCCGGGGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAA590.14825983163714035No Hit
GCGTCCGGGAAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTC590.14825983163714035Illumina Single End Adapter 2 (96% over 28bp)
GCGTCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACAT580.14574695313481595No Hit
GCGTCCGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA580.14574695313481595Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAA550.1382083176278427No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGCGCGGGCCG550.1382083176278427No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGAAGAGCTCGTA540.1356954391255183No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGCGGCCCGGGAA510.12815680361854503No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGGGCTCGTATGCCGTC510.12815680361854503Illumina Single End Adapter 2 (96% over 25bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGCAGGGCCTCGTATGCCGTC510.12815680361854503No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGAAGAGCTCGTA500.12564392511622063No Hit
GCGTCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA500.12564392511622063Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCAGAGCTCGGGTGC490.12313104661389623No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGCGTCCGGGGAC490.12313104661389623No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGGGCTCGTATGCCGTC480.1206181681115718No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTCGGAAGAGCTCGTAT480.1206181681115718No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1206181681115718No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCCGCGTCCGGGGAC480.1206181681115718No Hit
GCGTCCGGGCAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAA480.1206181681115718No Hit
GCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTAGTTGAAATGGTAA470.1181052896092474No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGGCG460.11559241110692298No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGGCAGGGCCGCGTGCCGGCTT450.11307953260459858No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAA450.11307953260459858No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTTCGGAAGAGCTCGTA450.11307953260459858No Hit
GCGTCCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA440.11056665410227415Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCTCGTATGCCGTCTTCTGCTT440.11056665410227415Illumina PCR Primer Index 9 (96% over 26bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGCGGCGCGGGTA420.10554089709762532No Hit
GCGTCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG420.10554089709762532No Hit
GCGTCCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA420.10554089709762532Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGGCCCGGATG410.10302801859530092No Hit
GCGTCCGGGATCACAGAAGGGCGGCAGCATCTCTGGGCTTTGCTTCTGTAGA410.10302801859530092No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTCCGGGGAG410.10302801859530092No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGCCCGGGCCG400.1005151400929765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT359.866562E-846.00000423
CAATGTG359.866562E-846.00000440
GGGTCTC359.866562E-846.0000048
TTCATGG359.866562E-846.00000427
TATATTT359.866562E-846.00000445
TTCGTTT359.866562E-846.00000421
TCGACGG359.866562E-846.00000433
AATTTTA359.866562E-846.00000440
ATAAGGT359.866562E-846.00000415
CTGGGTT359.866562E-846.00000439
GGCAATG359.866562E-846.00000438
CCCGGGC359.866562E-846.00000444
GGTCTCA359.866562E-846.0000049
GGCCGCG359.866562E-846.00000437
GACGGCA359.866562E-846.00000435
TTTCATG359.866562E-846.00000426
TTTTCAT359.866562E-846.00000425
CATTTTC359.866562E-846.00000423
GGTTCGT359.866562E-846.00000419
GGTTCGG359.866562E-846.00000442
Produced by FastQC (version 0.11.5)