FastQCFastQC Report
Fri 17 Jun 2016
SRR1527427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43285
Sequences flagged as poor quality0
Sequence length52
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGAAG16203.742636017095992No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC6281.4508490239112857Illumina Single End Adapter 2 (100% over 25bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGGAG5401.2475453390319973No Hit
GCGTCCGGGGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTAC5161.192098879519464No Hit
GCGTCCTTATGAATGTTTTGACCATTTTCATGGTGGAAGAATTTTATATTTA3850.8894536213468869No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA3620.8363174309807092No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAA3260.7531477417119095No Hit
GCGTCCGGGGAGGAAGGGCGTAGTAAGCGACGAAATGCTTCGGGGAGTTGAA2570.5937391706133764No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG2260.5221208270763544Illumina Single End Adapter 2 (100% over 23bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT2080.4805359824419545Illumina Single End Adapter 2 (95% over 24bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGGGCTCGTATGCCGTC1970.45512302183204345No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAA1960.45281275268568794No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGAAG1650.3811944091486658No Hit
GCGTCCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT1630.37657387085595473Illumina Single End Adapter 2 (100% over 29bp)
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGAAGCA1580.36502252512417693No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGAAGAGCTCGTA1380.3188171421970659No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAGGGGCTCGTATGCCGTC1240.28647337414808827No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG1190.2749220284163105No Hit
GCGTCCGGGGGAAACAAAGGAAAGCAGGAGGGAATTTCCTAAGATTGGATAC1160.26799122097724387No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGGGAAG1100.25412960609911056No Hit
GCGTCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA1040.24026799122097725Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGGTTGGACGCGTCCGGTGCCGGGGGTGCTGTTCCCTCGATCCCT1040.24026799122097725No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGC990.22871664548919948Illumina Single End Adapter 2 (95% over 21bp)
GCGTCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA950.2194755689037773No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT910.2102344923183551Illumina Single End Adapter 2 (96% over 26bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTTCGGAAGAGCTCGTA880.20330368487928843No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.19637287744022178No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGCAGAGTGCAGTGCTGCGTCCGGG840.19406260829386623No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGGAG840.19406260829386623No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT830.1917523391475107No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.18020099341573295No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGCGC780.18020099341573295No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGAAGAGCTCGTA720.1663393785375996No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAA720.1663393785375996No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGATCGGAAGAGCT710.1640291093912441No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTGCGGGAAG710.1640291093912441No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTCGGATGCCGTCT690.15940857109853296No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAAAA690.15940857109853296No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGCCCGGGAAG690.15940857109853296No Hit
GCGTCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACAT690.15940857109853296No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.15940857109853296No Hit
GCGTCCGGGATCACAGAAGGGCGGCAGCATCTCTGGGCTTTGCTTCTGTAGA680.15709830195217744No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGGCG680.15709830195217744No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGACG650.1501674945131108No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTT640.14785722536675522No Hit
GCGTCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA640.14785722536675522Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAA620.14323668707404413No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTAT610.1409264179276886No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGGCTCGTATGCCGTC600.13861614878133302No Hit
GCGTCCGGGGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAA580.13399561048862194No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGGAGGGGCTCGTATGCCGTC580.13399561048862194No Hit
GCGTCCGGGTTAATCTACACACTCAACAGTTCCAGTCAAAATTCAAACAGGG560.12937507219591082No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGGAAG560.12937507219591082No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAAAAA550.12706480304955528No Hit
GCGTCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG540.12475453390319972No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCC540.12475453390319972Illumina Single End Adapter 2 (95% over 22bp)
GCGTCCGGGAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAA530.12244426475684417No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGCAGAGCTCGTA520.12013399561048863No Hit
GCGTCCGGGAAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTC510.11782372646413307Illumina Single End Adapter 2 (96% over 28bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGAGCTCGTATGCCGTC510.11782372646413307Illumina Single End Adapter 2 (96% over 25bp)
GCGTCCGGGCAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAA480.11089291902506643No Hit
GCGTCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA470.10858264987871086Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGGATCGGAAGAGCTCGTATGCCGTCTT470.10858264987871086Illumina Single End Adapter 2 (96% over 27bp)
GCGTCCGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCT460.10627238073235533Illumina Single End Adapter 2 (100% over 26bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGAGAGCTCGTAG450.10396211158599977No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCTCGTATGCCGTC450.10396211158599977No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA440.10165184243964422No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCC253.362549E-546.045
TTTGACC452.9467628E-1046.017
GGAGAGT501.6370905E-1146.08
TTTTGAC452.9467628E-1046.016
ACCGGGG206.243993E-446.044
AACGCCG405.3987605E-946.023
TCCTTAT452.9467628E-1046.04
GGGTTGG253.362549E-546.08
ACGCCGG253.362549E-546.024
GCCTCGT550.046.038
TATATTT452.9467628E-1046.045
TCGATCC206.243993E-446.044
AATTTTA452.9467628E-1046.040
ATAAGGT600.046.015
TGCGTGC253.362549E-546.040
CGGGGTT253.362549E-546.06
ACCATTT405.3987605E-946.021
TGACCAT452.9467628E-1046.019
CCTCGTC206.243993E-446.039
CACGCAG206.243993E-446.023
Produced by FastQC (version 0.11.5)