FastQCFastQC Report
Fri 17 Jun 2016
SRR1527426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221177
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12280.5552114369939007No Hit
GCCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7650.3458768316777965No Hit
GCCTAGGGGGTTAGGGAGGTTGGGTGAGAGGGGTCACCTCCTCCCTACAT6920.31287159153076494No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGA6460.29207376897236154No Hit
GCCTAGGGGGCCCCGCGACACCTGCCCGCTCATCAGCCTGATACTCGCCA5960.26946744010453166No Hit
GCCTAGGGGCAGTGCTGCCTAGGGGCATGATGGGCAGGCTGCAGAGCTGT5410.24460047834991885No Hit
GCCTAGGGGATAGAGATGTTTTGAAAAAGTTTCAATGTAATTCTCTGGTC5090.23013242787450774No Hit
GCCTAGGGGACCCCTGGTTCTGCTTTCATGCTGATAATGTACTGAGACTT4950.2238026557915154No Hit
GCCTAGGGGATAAGAAATTCTCAAGAGCTCCAGAGAATCTCAAGTTTATT4920.22244627605944559No Hit
GCCTAGGGGAGACCAGTGAGATGCTCTCTCTCTCATTAGCACCTGACATC4730.21385587108967027No Hit
GCCTAGGGGGTTCCAGCCCATTCAGCAGGAGCGATCGCAGGAGCGGGGCT4560.20616971927460812No Hit
GCCTAGGGGCTCAGTGTTTGTAATTTGTACTATTGATGGGATAGTCTTGG4550.20571759269725154No Hit
GCCTAGGGGGATGAAGATGCTCCCATGATTACTGGATTCTCAGACGATGT4370.1975793143048328No Hit
GCCTAGGGGGATTTGATGGAAGCAAGAGAGCCAAGCCTCTCTTCTGAGTG4340.196222934572763No Hit
GCCTAGGGGAGGGTTTCTCTGTATAGTCCTGGCTGTCCTGGAACTCACTT4310.1948665548406932No Hit
GCCTAGGGAGAGGCCACGGCTTCATTTCTATGCGTAAAAAGCTCATTTAG4230.19124954222184043No Hit
GCCTAGGGGAAGATGGCGGATGAGAGTGAGACAGCAGTGAAGCTGCCGGC4080.18446764356149148No Hit
GCCTAGGGGGTCGCAAGGTCCGGGAAGGACGTTTGAAGAAGAAGCAGATC3980.1799463777879255No Hit
GCCTAGGGCCAACAGACATCCCACCCAGGCCCACGCAGCAAACAGGAACA3900.17632936516907274No Hit
GCCTAGGGGGAGGCTGGGCCTCTTCCCGATGCATAGGTGTGACCCATAGC3680.16638258046722762No Hit
GCCTAGGGGGTCTGGTCCCCAGCCTGGGGGGGTTCTGAGCAGTGCCTCTT3680.16638258046722762No Hit
GCCTAGGGGGTGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGC3670.16593045388987102No Hit
GCCTAGGGGAATGATTTTTCACGTGTATGTCTGACTTGCCTGGCAAGACT3650.1650262007351578No Hit
GCCTAGGGGAGTTCTCACGGAGTCTGGCAGGAGTGCTCACCAAGTGGCCA3610.16321769442573142No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTG3590.16231344127101824No Hit
GCCTAGGGGACCCTCTCCCTGCTTGAGCATCGCCCGGGAGCTGAGAGGAG3520.15914855522952207No Hit
GCCTAGGGGGTGTCCCTTCTTCCTGTCTCCCTGTCATCTGTCTGGGAGTC3480.15734004892009568No Hit
GCCTAGGGGACCAGGGAGGCATTCTCCCAGGGGAGCTGCTTCCCCATCAC3440.15553154261066926No Hit
GCCTAGGGGAGAAAACAGTGGGCTGTCTTCACTTCCAGTTGCAGCTCCTC3440.15553154261066926No Hit
GCCTAGGGGGGGCATCCGCCGTCTCTACCATAGGGATGCGGTGCTCCGTG3420.1546272894559561No Hit
GCCTAGGGGGTGGGGTTTGTCATTTTGTTAGGCCCTTGCCTGTCTGCCCA3420.1546272894559561No Hit
GCCTAGGGGATTCTTGTTTTTGTTTTGTGTGGTGGGTTCACTGACCGGCG3400.1537230363012429No Hit
GCCTAGGGGATGCCCTCTCCTGCTGGGAGGGCCCTGTTTTCTGGATTTGA3380.15281878314652972No Hit
GCCTAGGGGACCTGAGGCTTTGCCTCCTGCCAGGACCTGCCCCACCCCTG3340.15101027683710333No Hit
GCCTAGGGGGACCTGAAGGAAGCAGGCTGGCACTAGCAGTTGCCTTGTCT3310.14965389710503355No Hit
GCCTAGAGGGACCCGGGCAAAGGCTGTGCGGGCCCTGAAGGACAACCACA3300.1492017705276769No Hit
GCCTAGGGGGAGCTGCCTTGCCTGTCAGCTGCTCCTTCGAGGCCTGTGTC3290.14874964395032034No Hit
GCCTAGGGGGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAAC3290.14874964395032034No Hit
GCCTAGGGGGTGCAGTGCTGCCTAGGGAGAAAGGAAAATATAATGAATGA3280.14829751737296373No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3240.14648901106353734No Hit
GCCTAGGGGATTTAACCTTCATTCATCCTTAACGGTGTGGAAGGGTTGAT3150.14241987186732796No Hit
GCCTAGGGGACACACGAACGGGATGGGCTCCAGCAAGGCTCGCTTAGTTC3140.1419677452899714No Hit
GCCTAGGGGAGGAAGTTTTTGGACGGCATCTATGTGTCTGAGAAGGGAAC3110.14061136555790157No Hit
GCCTAGGGGAGAGCTGGCCTCCCAGAAAGATGGAGGCATCAAGAGTGGGA3090.1397071124031884No Hit
GCCTAGGGGTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCG3070.13880285924847519No Hit
GCCTAGGGGGCTGCCTAGGGGGTCCCCACTCCCACCAGCCTACCACCTAT3070.13880285924847519No Hit
GCCTAGGGGATTTCCAAATTTGCACGAACTTTTTACCACAACATGTGCCT3060.1383507326711186No Hit
GCCTAGGGGGAGCGCCTTGGGTACTTCTCTGTGGATCCAGACAGCCATCA3030.13699435293904882No Hit
GCCTAGGGGAGCTTTCTTGTCCTTGTAAGAGGTGGATAAAGATACCCAGG2990.13518584662962244No Hit
GCCTAGGGGATGTTGAAAGTGTTAGATGGCTAAGTATTAAAAGCACCCAG2970.13428159347490923No Hit
GCCTAGGGGACCTCCTCTGGGTTCTCCAAAGCCCAAGGTGTTTTTGTCCA2970.13428159347490923No Hit
GCCTAGGGGATCCCCACCCCCGGCGCTGCGAGCACAATCCCGTTGATTTG2960.13382946689755262No Hit
GCCTAGGGGAACCCTGTCTCTACTAAAAAGCAAATACAAAAACTAGCCGG2960.13382946689755262No Hit
GCCTAGGGGGGTGAACTTGGGCGGCTGTGGAGGCCGCTCTCCCTGTGGTG2950.13337734032019605No Hit
GCCTAGGGGGTCTGGGGTCCAGGCCATCCCAAGAGAAGAATACTGGTTGT2940.13292521374283944No Hit
GCCTAGGGGGTATGCCAAGCGTGCCTTTGTGCACTGGTATGTGGGTGAGG2940.13292521374283944No Hit
GCCTAGGGGCAGCGCCAGCACCCCCTGCTGGCTCCTTTTACCCTATGGTC2920.13202096058812626No Hit
GCCTAGGGGACGAATAAATGAATTCACTAATGTTCATGTGCAACCTCATG2910.13156883401076966No Hit
GCCTAGGGGCGGCCTCTTTTTGGGTTCCCGGAGGGTGGAAAGGAATGGGC2910.13156883401076966No Hit
GCCTAGGGGGAAATGTCAAAACTAACACAGCATGTGTTATTTTGCAGAAG2880.13021245427869987No Hit
GCCTAGGGGCGTCCCCACCCCTGGCCCTGCCCAGCCTGTGTAGCTGTTCT2870.12976032770134327No Hit
GCCTAGGGGAGTGTTCCTGGCCCAGATGGCCCCTGCTGGCTATTGGGTTT2860.12930820112398667No Hit
GCCTAGGGGGGGGTACTTCTGGAACAGAAGGAGCTCTCAAAGGCGCTCTG2860.12930820112398667No Hit
GCCTAGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAGCACTGACTGCTC2840.12840394796927349No Hit
GCCTAGGGGTGCAGTTTTGAAGTTGTTTCTCCCTAACTCCCAACTCCCAA2840.12840394796927349No Hit
GCCTAGGGAATGGACTGCAGTGCGCATAACCAGCTCACCTCTTAGCCCAA2830.12795182139191688No Hit
GCCTAGGGCGCAAGGGGATATGAAACACTGCCTTTTGCTCTTCTGGGGAC2830.12795182139191688No Hit
GCCTAGGGGCACACACAGCCTGACTGTAAGCCGTTGTTGGGAGGAGTCCA2820.12749969481456028No Hit
GCCTAGGGGGGCCAGGGTGTCACCAGGGTGACGTACCCTCTCACTGCTGA2800.1265954416598471No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC2800.1265954416598471No Hit
GCCTAGGGGCAGAGTGCAGTGCTGCCTAGGGGACCTGCTTCCTGGGAAAG2770.1252390619277773No Hit
GCCTAGGGGGACCCCCTGCCAGGCGCATGTCTGCACCTCCGCTTGTAAGA2770.1252390619277773No Hit
GCCTAGGGGAAAGCAATCTGCTGAGAAAGCAGAGCTGGCCAAAGGGACAG2770.1252390619277773No Hit
GCCTAGGGGCCTCCACCTCCAGAGTTTGTGGTGTTACAGTGGTATCTGCA2750.12433480877306412No Hit
GCCTAGGGGATAGGCCCCCTGGCCAAATGCATTTCTAGCAGCCTTGCGTG2740.1238826821957075No Hit
GCCTAGGGGGCTGCCTAGGGGAGACAAAGCACAGCAGAACAACGTGGAAC2740.1238826821957075No Hit
GCCTAGGGGTACTTGCTTAGTGACTCCAGGCTGCATCATATATCATAATT2730.12343055561835092No Hit
GCCTAGGGGACAGGAGACTTTTCTCGATTAACCGCCCTGCCCGAGCAGCG2710.12252630246363773No Hit
GCCTAGGGGGACTTCCAAAGAGTCTCCGGCTGTCCCATCTCTGTCTGTTC2710.12252630246363773No Hit
GCCTAGGGGATTCTAGGCTAAGAAGAATCTTCTGCCAACTCCTCAAGCCA2700.12207417588628111No Hit
GCCTAGGGGAGCAAAAGATATAGATATATGGATAATATTAAAAAAAGCCT2670.12071779615421133No Hit
GCCTAGGGGGCTTTTATGATATTTGTGTGCAATATCCCCTCTTCTATCAA2670.12071779615421133No Hit
GCCTAGGGGCAAAAAGGTTGTGAAAATCGAGTCTTTAATGTCCTTTGAAG2670.12071779615421133No Hit
GCCTAGGGGGAAGTTGATTTTGACCTTAAGTGATCTGTATGCAAGTTGGA2660.12026566957685475No Hit
GCCTAGGGGAACCATACTCAGGACCCTTGAGCACCTCACTCCTGTGGCCA2660.12026566957685475No Hit
GCCTAGGGCCCAGTAGGATGTGGAGTGTGAGCCTGTATCCTTGGCCCCAG2650.11981354299949813No Hit
GCCTAGGGGAGCGCTGCCGAGCTGCGTCACGTCATGACGAACCTGGGGGA2630.11890928984478494No Hit
GCCTAGGGGACTGTCCCCCTTTCTAATGGCCACTCTTTAGTGTTTGTGTC2630.11890928984478494No Hit
GCCTAGGGGGCTGCCTAGGGGGTTCCTATGAAAGCCAAGAGTGTGGCTGA2630.11890928984478494No Hit
GCCTAGGGGGAGTGCTGTGCTGCCTAGGGGGAGGGGTGGCTATTAATTAT2610.11800503669007174No Hit
GCCTAGGGGAGCCACCTCTGTATTTATGTTGCTTATTCGTCTGTTTTTAT2600.11755291011271515No Hit
GCCTAGGGGGGTTCCGGCTGAGCTCTCCTGGCTGGTCCCCCAGGACCACC2600.11755291011271515No Hit
GCCTAGGGGACAGCGCAGGCCACTGAGTGGGTTGGAGCCACCACTGAGTG2590.11710078353535855No Hit
GCCTAGGGGATGTCTTTACTGTCCTTACAAGCACAGTGTGTGTTACCACA2590.11710078353535855No Hit
GCCTAGGGGAGGCTGAGGTTTTCTCATTACCCACCTGGAATCTGGTGAAT2570.11619653038064535No Hit
GCCTAGGGGGAAGTGTCACTCCCTGAAGATGAAGCATTCAAATATGAGTC2570.11619653038064535No Hit
GCCTAGGGGCATCAGCCGCAGACCGGGCCCTGTTTACCCATCACCCTCAC2570.11619653038064535No Hit
GCCTAGGGGATGTCTACAAGAATCTTCTATCCTGTCCTGTGCCTTAAGGC2550.11529227722593216No Hit
GCCTAGGGGATAGTATCCCCGTGGCTCCCCCAGCAGCCCTGACCCACCTG2550.11529227722593216No Hit
GCCTAGGGGAAGCTTATGCCCCCTTGGCTGTGTATAGCAAACTGTTTTTA2550.11529227722593216No Hit
GCCTAGGGGCCTGCTGAGGGCCAGGCCCCTCACGCCCTCGCCCCAGGCCC2550.11529227722593216No Hit
GCCTAGGGGAAGCCTCACGTGTGTGCCTTTATGAGGCGTTGGGAGAAAGC2550.11529227722593216No Hit
GCCTAGGGGATGCTGGAATTTGTCCAGCAATGAGGACACAGCTAATGGGG2540.11484015064857557No Hit
GCCTAGGGGATGTGTGGGTGACATCCCCACACCCACCCGGGCAGCCCCTA2520.11393589749386238No Hit
GCCTAGGGGAGGAAATATGTCTCTGAATGCATGAAATCATGTTGATTTTT2510.1134837709165058No Hit
GCCTAGGGGGGTCTGAGGAAAAACCAGCGCATCAGTGGGTTCTTGGGCAG2490.11257951776179259No Hit
GCCTAGGGGATTCATTATTGACATTTGTAGTTGAGGGATCCGGGGGTTCA2480.11212739118443599No Hit
GCCTAGGGGACTGACTTGGTCTATGTAAGAGCCATTGCACTAAGTTAAAT2480.11212739118443599No Hit
GCCTAGGGGATTAGTTGCAGGCACTGATGCTTCTGTGCTGTTTCCATTCT2480.11212739118443599No Hit
GCCTAGGGGGAATGAATGAAATTCCCAAGATATAAATTGTATAGTTTTTC2470.1116752646070794No Hit
GCCTAGGGGAAAGCGCAGAGGTGGATCCCATTCTTTTGACCATGAGAAAA2470.1116752646070794No Hit
GCCTAGGGATGATCCTCATTCCTTCCACCAATCAAAACTTACCAATGAAG2460.11122313802972279No Hit
GCCTAGGGCAGCAGCATGTTTTCCTGTTTCGTGACTATATAGAGATGTTT2450.1107710114523662No Hit
GCCTAGGGGAGTGCTGCCTAGGGGAGCCCGCCCTGGGACCTCCACCTGAA2440.1103188848750096No Hit
GCCTAGGGGGGGCTCAGTGCTGAGAGAGAGGCAGGGCCTTGCTGGGTGCC2440.1103188848750096No Hit
GCCTAGGGGATGGAGACTTTGTCTTGGGTAGGGTGGCTCCGCAGTTGGAC2430.10986675829765301No Hit
GCCTAGGGGATTGATTTACATAGTCCTTCTGAGATTGTTAAGCAGATTAC2420.10941463172029642No Hit
GCCTAGGGGGGAGAGACAACTCTCAGTTTAAAAAGTATGGCTCTTGCAGA2420.10941463172029642No Hit
GCCTAGGGGGGTAGTCCTTTTGGGAAAAATCCAGTATTATCTAAAATTAT2410.10896250514293981No Hit
GCCTAGGGGCAGAGTAGAAGATGACAGATGGAAAAGATCCAGGCCTGCAA2400.10851037856558322No Hit
GCCTAGGGGGCTGCCTAGGGGGTAAAGCATAAATAAAAAGCCTTTGTGGA2400.10851037856558322No Hit
GCCTAGGGGAGTGTCAGATCTGGTACATGAATGGCCATTCTACCCAGATA2380.10760612541087003No Hit
GCCTAGGGGTCTCTGTTCTTGATGAGGAATGTACTGCTTTGTTCATGCTG2370.10715399883351343No Hit
GCCTAGGGGGGCGTGTCCACGTCACTCAGGAGGACTTTGAGATGGCAGTA2360.10670187225615684No Hit
GCCTAGGGGAAGCTATCGGACTGTGTGCTGCCATCGGTAATGAGTCTCAG2360.10670187225615684No Hit
GCCTAGGGGGTTCCACGCTATTTAATTGCAGTGTACAGAGTCGTGTTTGT2350.10624974567880023No Hit
GCCTAGGGGAGTCGAAAAGGAAGAATCCAGCCATGTATGAAAATGACAAG2330.10534549252408704No Hit
GCCTAGGGGATAATGGATCATGCGATATCAGTATGGGAAGTGTATGACAT2330.10534549252408704No Hit
GCCTAGGGGTGAAGGGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCC2320.10489336594673045No Hit
GCCTAGGGGAGTGCAGTGCTGCCTAGGGGGGCCGGGGTGAACTTCTGGTC2320.10489336594673045No Hit
GCCTAGGGGGTGCTGCCTAGGGGAAGGTGGCCAAACAGGAAAAGAAGAAG2310.10444123936937384No Hit
GCCTAGGGGCTTCTTCCCACTCCTCAGGGCCTCACGCACCTGTGCCTGGC2310.10444123936937384No Hit
GCCTAGGGGACATCCGATGATTGTTTTGGTTTCTATTCCCTTTCTAACTG2300.10398911279201725No Hit
GCCTAGGGGCCCTATCCCAACTCGGCCCCCAACACTGAGCATCTCCCTCA2290.10353698621466066No Hit
GCCTAGGGGGTACAGATGATGACTGCAAAATGAAGACCTACTTTCAACTA2290.10353698621466066No Hit
GCCTAGGGGGTGCCACCTGTGAAGCAAGTCTCTCTCTAGTGTGGATGACC2290.10353698621466066No Hit
GCCTAGGGTAGGCTGAGCACTTTCCCTCCTGTGTGTCTCGTACCTTGCTC2290.10353698621466066No Hit
GCCTAGGGGAGCTTATGAAGCCCCTTCACTGGCCACTGGTTATGGTGCAT2280.10308485963730406No Hit
GCCTAGGGGAGATGCGCCCTAGGCAATGGTTGTTCTCTTTAAAGAGGGAA2280.10308485963730406No Hit
GCCTAGGGGATGTACCAAATTTAACTTGGCAAACTTTTATGGCCTGTCCC2280.10308485963730406No Hit
GCCTAGGGGACGGACATGATGTGCTCCCTTCCTGTTCTCACCCCCTAAAG2280.10308485963730406No Hit
GCCTAGGGGGAGTGAGGAGAGGCTGAGCATTCTACTACAGGACAGGACCT2280.10308485963730406No Hit
GCCTAGGGGAGCTTTGCCAGTGTTCCTTGTGATCCTCTTCTGCTGTTCTG2280.10308485963730406No Hit
GCCTAGGGGAACACGGGGGTGGGGGCAGAGCTGTGCGTCCTGCCCAGAAG2270.10263273305994747No Hit
GCCTAGGGGAGGCCTATTTCGATGTAAGGGTGGAGATGTATTTGATGGAA2270.10263273305994747No Hit
GCCTAGGGGATCTCAGGGGCCACAATAAGAAACATACTCTCCATAGTGCC2260.10218060648259086No Hit
GCCTAGGGGAGTTTGGGCAAAGAGATTTAGTTTGATTTGACTGATTGCCT2250.10172847990523427No Hit
GCCTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.10127635332787767No Hit
GCCTAGGGGAAGGACAAAGTGGTAAAAAAGGAAATAAAGACAAAACTCGA2220.10037210017316447No Hit
GCCTAGGGGAGACCATGGAGGAGAAGAAAGCATTTATGGGACCACTCAAG2220.10037210017316447No Hit
GCCTAGGGGAGAGTGCCCTGCAGGTTGCCCAAGTTCTCTGTCCCTAGCCC2220.10037210017316447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACTC207.842679E-444.00000416
GTCATTC207.842679E-444.00000436
CTAGATC408.260031E-944.0000043
AGCGTTC207.842679E-444.00000413
CTATGTC207.842679E-444.00000425
CGGAATC207.842679E-444.00000435
GGGTGTA207.842679E-444.00000414
TTAGGGT207.842679E-444.00000411
AGCGTCC408.260031E-944.00000439
AGCCGAC207.842679E-444.00000436
TACCCTA207.842679E-444.00000439
CAAACTC207.842679E-444.00000418
CAAACGG408.260031E-944.00000435
CCGGGCG207.842679E-444.00000419
CCGGGAT207.842679E-444.00000413
ACCGGCG207.842679E-444.00000444
CGAACAG207.842679E-444.00000437
TCACCGT207.842679E-444.00000429
ACGCCAA207.842679E-444.00000416
GGTAATG408.260031E-944.00000436
Produced by FastQC (version 0.11.5)