FastQCFastQC Report
Fri 17 Jun 2016
SRR1527423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569099
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38570.6777379682621125No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT23960.42101637852113605No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG22360.3929017622592906No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC14790.25988448407043413No Hit
TTCATAGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATCA12280.21577967980966403No Hit
TTCATAGGGGCCGACCCTTTCCCCATCTTAGCTGCCCTTGCCTGGCTTTC12060.2119139200736603No Hit
TTCATAGGGGCCCTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCA10810.18994937611909352No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10340.1816907075921764No Hit
TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG10070.17694636609798997No Hit
TTCATAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8920.15673898565978855No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT8560.15041319700087333No Hit
TTCATAGGGCTCCACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCC8200.14408740834195807No Hit
TTCATAGGGATTCTGGGCTCTGCCTCCTGCCTACACCACCATAGCCAGTG8100.14233024482559273No Hit
TTCATAGGGGCAAACCTGGCCACCAGGACCTTCCCTCTGACAAGAGGACA8060.1416273794190466No Hit
TTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7830.13758590333140633No Hit
TTCATAGGGAGGCTGGGCAGTGGTGGCACACGCCTTTAATCGCAGCACTC7780.13670732157322366No Hit
TTCATAGGGGTCGGGCGGTGGTGGCTCACACCTTTAATCCCAGCACTCGG7350.12915151845285266No Hit
TTCATAGGGGTTCTGTGCCCTCCCTTTCAAAAGCTCTGAGCCCCGCTCAT7210.1266914895299412No Hit
TTCATAGGGGTCCCCACCCACGGGGCCGTGGGGCTTTCCTGACTTGCACT6890.12106856627757209No Hit
TTCATAGGGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTAG6820.11983855181611634No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCGGTGGTCAAGAGCACTCTCTGCT6790.11931140276120675No Hit
TTCATAGGGGGCAGAAGGGTCAAGGAAAGCCCTCAGCCTCACCTCCTCAT6330.11122845058592616No Hit
TTCATAGGGGGGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGGG6310.11087701788265311No Hit
TTCATAGGGGAGTGCAGTGCTTTCATAGGGAAAAAAAAAAAAAAAAAAAA6290.11052558517938003No Hit
TTCATAGGGGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACT6280.11034986882774352No Hit
TTCATAGGGGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATC5980.10507837827864748No Hit
TTCATAGGGCAGTGCTTTCATAGGGAGCCACTTGCCCCAATTTAAGTTTA5900.1036726474655552No Hit
TTCATAGGGGTGAAGGCAATGCCCTCAAAACTATTTTACTAAACTGAATT5840.102618349355736No Hit
TTCATAGGGATTTTGTTTTCTTGTAGGCCAGGCTGACTATTCACAGTATA5750.1010369021910072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATC950.044.00000420
TCACGAC254.439997E-544.040
CTTCGAT207.853127E-444.027
ACTATCG207.853127E-444.030
CGAACTC551.8189894E-1244.026
TCGCACC207.853127E-444.015
CCGGGCA900.044.017
CGAACCC207.853127E-444.038
GATAATC302.5254558E-644.039
TCCGCGT207.853127E-444.041
CTCGTAG302.5254558E-644.021
GGTCGTA207.853127E-444.09
CGAAATT207.853127E-444.032
GGTCGAC551.8189894E-1244.09
TGCCACG502.7284841E-1144.020
CGAAAAT408.300049E-944.033
CGACGGG254.439997E-544.037
CGACGCG408.300049E-944.031
ACTTGCG600.044.031
GCGAACT551.8189894E-1244.025
Produced by FastQC (version 0.11.5)