FastQCFastQC Report
Fri 17 Jun 2016
SRR1527422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478552
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC31830.6651314799645597No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC20810.4348534746485231No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT16220.33893913305137163No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA13450.28105618616158745No Hit
TTCATAGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATCATT10570.22087463849278657No Hit
TTCATAGGGGCCGACCCTTTCCCCATCTTAGCTGCCCTTGCCTGGCTTTCTC10440.21815811029940319No Hit
TTCATAGGGGCCCTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACC9170.19161971948711948No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8560.17887293334893595No Hit
TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8330.1740667680837192No Hit
TTCATAGGGATTCTGGGCTCTGCCTCCTGCCTACACCACCATAGCCAGTGCC8310.17364884066935254No Hit
TTCATAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7330.15317039736538557No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCGGTGGTCAAGAGCACTCTCTGCTCT7150.1494090506360855No Hit
TTCATAGGGGCAAACCTGGCCACCAGGACCTTCCCTCTGACAAGAGGACACT7090.1481552683929855No Hit
TTCATAGGGCTCCACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTG7010.14648355873551883No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT6970.14564770390678547No Hit
TTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6640.13875190156973535No Hit
TTCATAGGGGTTCTGTGCCCTCCCTTTCAAAAGCTCTGAGCCCCGCTCATGC6600.13791604674100202No Hit
TTCATAGGGGTCGGGCGGTGGTGGCTCACACCTTTAATCCCAGCACTCGGGA6400.1337367725973353No Hit
TTCATAGGGGGGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGAG6140.12830371621056855No Hit
TTCATAGGGGAGTGCAGTGCTTTCATAGGGAAAAAAAAAAAAAAAAAAAAAA5890.12307962353098514No Hit
TTCATAGGGGTCCCCACCCACGGGGCCGTGGGGCTTTCCTGACTTGCACTGC5780.12078102275196845No Hit
TTCATAGGGAGGCTGGGCAGTGGTGGCACACGCCTTTAATCGCAGCACTCAG5740.11994516792323509No Hit
TTCATAGGGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTAGGG5710.11931827680168508No Hit
TTCATAGGGGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCT5390.11263143817181831No Hit
TTCATAGGGGGCAGAAGGGTCAAGGAAAGCCCTCAGCCTCACCTCCTCATGG5370.11221351075745163No Hit
TTCATAGGGCAGTGCTTTCATAGGGAGCCACTTGCCCCAATTTAAGTTTAGA5270.1101238736856183No Hit
TTCATAGGGGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATCTC5090.10636252695631822No Hit
TTCATAGGGGTTGATCTCAGCCCCTACTCCCAGACTGCTGAGGTAACTACAG4940.10322807134856818No Hit
TTCATAGGGATTTTGTTTTCTTGTAGGCCAGGCTGACTATTCACAGTATAGC4940.10322807134856818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGA351.0178883E-746.00000435
GCGTTAT351.0178883E-746.00000432
GTTCGTA351.0178883E-746.00000430
CACTACG351.0178883E-746.00000433
CCGCGTT351.0178883E-746.00000432
CGACTTG351.0178883E-746.00000429
CGACTAG351.0178883E-746.00000438
TCACGCT253.4141456E-546.015
TCACGAC405.5988494E-946.040
AACGTTA206.3077436E-446.027
CGGCGTA206.3077436E-446.031
CGTGGCG405.5988494E-946.027
TAATAGC301.8591109E-646.018
CCTTATA405.5988494E-946.025
TCGCATC206.3077436E-446.045
AGCCGAA301.8591109E-646.010
AATCCCG253.4141456E-546.036
CAAACCG551.8189894E-1246.010
GGTAATC301.8591109E-646.031
GGTAACG206.3077436E-446.09
Produced by FastQC (version 0.11.5)