##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527422_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478552 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84743977665959 33.0 31.0 33.0 30.0 34.0 2 32.34922014744479 33.0 31.0 34.0 31.0 34.0 3 32.794089252578615 34.0 31.0 34.0 31.0 34.0 4 35.62136403149501 37.0 35.0 37.0 35.0 37.0 5 36.13583476821746 37.0 35.0 37.0 35.0 37.0 6 35.53637640214647 36.0 35.0 37.0 35.0 37.0 7 36.357022852271015 37.0 36.0 37.0 35.0 37.0 8 36.68252352931343 37.0 37.0 37.0 35.0 37.0 9 38.72070119861583 39.0 39.0 39.0 39.0 39.0 10 37.61932036643876 39.0 37.0 39.0 35.0 39.0 11 37.724124024139485 39.0 37.0 39.0 35.0 39.0 12 37.671540396863875 39.0 37.0 39.0 35.0 39.0 13 37.64119886658085 39.0 37.0 39.0 35.0 39.0 14 38.42398736187499 40.0 38.0 40.0 35.0 40.0 15 38.51396295491399 40.0 38.0 40.0 35.0 40.0 16 38.59982196292148 40.0 38.0 40.0 35.0 40.0 17 38.56760184891088 40.0 38.0 40.0 35.0 40.0 18 38.42346286296996 40.0 38.0 40.0 35.0 40.0 19 38.34671049332152 40.0 38.0 40.0 34.0 40.0 20 38.34526864374195 40.0 38.0 40.0 34.0 40.0 21 38.311738327287316 40.0 38.0 40.0 34.0 40.0 22 38.34643257159097 40.0 38.0 40.0 35.0 40.0 23 38.23941807786824 40.0 38.0 40.0 34.0 40.0 24 38.20487428743376 40.0 38.0 40.0 34.0 40.0 25 38.136515989902875 40.0 38.0 40.0 35.0 40.0 26 38.10144143165215 40.0 38.0 40.0 35.0 40.0 27 38.05318335311523 40.0 38.0 40.0 35.0 40.0 28 37.989029405372875 40.0 38.0 40.0 34.0 40.0 29 37.93339699760946 40.0 37.0 40.0 34.0 40.0 30 37.796768167304705 40.0 37.0 40.0 34.0 40.0 31 37.78860395526505 40.0 37.0 40.0 34.0 40.0 32 37.698350440495496 40.0 37.0 40.0 34.0 40.0 33 37.55910329493973 40.0 37.0 40.0 34.0 40.0 34 37.30421354419165 40.0 36.0 40.0 33.0 40.0 35 37.27267465186647 40.0 36.0 40.0 33.0 40.0 36 37.19276901987663 39.0 36.0 40.0 33.0 40.0 37 37.21742882696133 39.0 36.0 40.0 33.0 40.0 38 37.291700797405504 40.0 36.0 40.0 33.0 40.0 39 37.201884016783964 40.0 36.0 40.0 33.0 40.0 40 37.07703238101607 40.0 36.0 40.0 33.0 40.0 41 36.964595279091924 40.0 35.0 40.0 33.0 40.0 42 36.88067963356124 39.0 35.0 40.0 33.0 40.0 43 36.83954094852806 39.0 35.0 40.0 33.0 40.0 44 36.77533475985891 39.0 35.0 40.0 33.0 40.0 45 36.65055417175145 39.0 35.0 40.0 32.0 40.0 46 36.54856316554941 39.0 35.0 40.0 32.0 40.0 47 36.409506176967184 39.0 35.0 40.0 31.0 40.0 48 36.26500777344991 39.0 35.0 40.0 31.0 40.0 49 36.21087990437821 39.0 35.0 40.0 31.0 40.0 50 36.416916030023906 39.0 35.0 40.0 32.0 40.0 51 36.45822606529698 39.0 35.0 40.0 32.0 40.0 52 36.10769780504522 39.0 35.0 40.0 31.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 3.0 14 6.0 15 13.0 16 28.0 17 42.0 18 79.0 19 133.0 20 181.0 21 285.0 22 485.0 23 760.0 24 1157.0 25 1654.0 26 2119.0 27 2757.0 28 3556.0 29 4056.0 30 4806.0 31 6421.0 32 8951.0 33 12933.0 34 27369.0 35 25430.0 36 38257.0 37 72019.0 38 198591.0 39 66460.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.64726926227453 0.2607867065648038 0.054539527574850796 0.03740450358581721 8 99.07554455942092 0.8095254016282453 0.09466055935405139 0.02026947959678363 9 97.85039034420502 1.341755963824203 0.2574432872498705 0.5504104047209081 10 48.605585181965594 41.13972985171935 3.5578160785034854 6.696868887811565 11 32.96026346144202 23.438205252511743 27.224209699259433 16.377321586786806 12 31.191176716427893 20.07660609505341 25.27186178304552 23.460355405473177 13 24.894473327872415 20.294346277938445 29.185334091175047 25.625846303014093 14 26.2176315217573 22.364758688710946 28.930398368411375 22.487211421120378 15 25.920903057556966 22.415954796970862 28.447692204817866 23.215449940654306 16 28.982430331500026 24.865636336281113 25.44530166000769 20.70663167221117 17 29.40683562078938 23.675796987579197 24.104799478426585 22.812567913204834 18 26.443312325515304 23.567971714672595 27.635032347581873 22.353683612230228 19 26.703681104665744 24.70055500760628 26.85141844564436 21.744345442083617 20 25.14105050234875 26.130284692154664 26.311874153696984 22.416790651799595 21 24.86166602584463 23.899388154265367 30.35365017803708 20.88529564185292 22 26.164345776425552 23.752068740701116 28.005733964125113 22.077851518748222 23 25.312609705946272 24.52523445727946 28.76573496715091 21.396420869623363 24 25.72092478978251 24.15766729634397 26.89509186044568 23.22631605342784 25 24.642672060716496 28.16412845417008 24.572042327688525 22.621157157424896 26 25.13519951854762 25.61769671843394 25.409150938664972 23.83795282435347 27 23.977540580751935 25.951620722512914 24.61237232317491 25.458466373560242 28 25.661161169528075 26.355756532205486 25.258070178371423 22.725012119895016 29 27.190148614988548 26.359308915227604 24.741720857921397 21.708821611862454 30 27.488130861431987 24.851635767899833 24.74255671275013 22.917676657918054 31 27.374454604724253 25.36944783430014 24.868143900767315 22.387953660208296 32 27.793844765041207 26.629290024908475 24.057573680603152 21.519291529447166 33 27.956627492937024 23.823116401143448 26.496598070847057 21.723658035072468 34 24.996656580685066 25.947023520954883 27.677869907554452 21.3784499908056 35 24.288896504455106 29.260561025761046 25.059136729132884 21.391405740650963 36 23.669319112656513 29.5946939935472 25.175111586619636 21.560875307176648 37 25.23989033584647 28.70722512913957 24.585624968655445 21.467259566358514 38 24.9322957588726 29.24676942108694 24.419707785151875 21.40122703488858 39 25.415837777294843 28.059019709456862 23.220882997041073 23.304259516207225 40 25.352312810311105 28.021615205871043 22.97639545963657 23.649676524181277 41 22.789999832829032 27.89352045336766 23.882462093983516 25.434017619819787 42 21.981937177151075 28.174367675822065 24.45063441381501 25.393060733211858 43 22.75008776475702 26.079297547601932 26.16539059496147 25.005224092679583 44 24.34845116100236 25.962904762700813 24.815067119142746 24.873576957154082 45 24.55156388438456 28.214070780186894 23.70860428960698 23.52576104582156 46 23.02591985823902 28.63513265016132 23.41918955515806 24.9197579364416 47 23.542896069810595 27.259524565773418 26.084521640281515 23.11305772413447 48 23.02654674936057 27.62562898075862 26.52564402614554 22.822180243735268 49 25.810988147578527 25.084421337702068 25.247621993012253 23.85696852170715 50 22.948603286581186 27.663869339173175 25.40204617262074 23.985481201624903 51 23.15861181230044 27.501295574984535 25.942844246811212 23.39724836590381 52 23.759382470452532 27.45135324896772 24.86396462662365 23.9252996539561 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 3.5 2 7.0 3 16.5 4 26.0 5 40.0 6 54.0 7 479.0 8 904.0 9 1361.0 10 1818.0 11 2174.0 12 2530.0 13 2096.0 14 1396.5 15 1131.0 16 1405.5 17 1680.0 18 1699.5 19 1719.0 20 2281.5 21 2844.0 22 3637.5 23 4431.0 24 5000.5 25 5570.0 26 6041.0 27 6512.0 28 7854.5 29 9197.0 30 9527.5 31 9858.0 32 9993.5 33 10129.0 34 11840.0 35 13551.0 36 14136.5 37 14722.0 38 15961.0 39 19680.0 40 22160.0 41 22631.0 42 23102.0 43 23140.0 44 23178.0 45 24986.5 46 26795.0 47 27681.0 48 28567.0 49 30079.5 50 31592.0 51 33111.5 52 34631.0 53 34629.0 54 34627.0 55 34322.0 56 34017.0 57 32575.5 58 31134.0 59 30769.5 60 30405.0 61 25593.5 62 20782.0 63 16417.0 64 10529.5 65 9007.0 66 6617.5 67 4228.0 68 3738.0 69 3248.0 70 2526.0 71 1804.0 72 1305.5 73 807.0 74 557.0 75 307.0 76 256.0 77 205.0 78 264.0 79 323.0 80 171.5 81 20.0 82 14.0 83 8.0 84 6.0 85 4.0 86 3.0 87 2.0 88 1.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 478552.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.170255270064695 #Duplication Level Percentage of deduplicated Percentage of total 1 70.54137119898354 8.585064945920193 2 8.967909204855685 2.182834885237132 3 3.2880616747651996 1.2004964977682675 4 1.5504541474219191 0.7547769103462111 5 0.9271818821792208 0.5642020093950083 6 0.750330523170962 0.547902840234708 7 0.6249892687282156 0.5324395259031411 8 0.523686063082708 0.5098714455273409 9 0.4361188853213372 0.477691034621107 >10 8.622791504266754 26.916406158578376 >50 2.2664446008825396 18.858138718467377 >100 1.454301952232963 33.786923887059295 >500 0.03605707319585859 2.9242381183236095 >1k 0.010302020913102453 2.1590130226182316 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 3183 0.6651314799645597 No Hit TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 2081 0.4348534746485231 No Hit TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 1622 0.33893913305137163 No Hit TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 1345 0.28105618616158745 No Hit TTCATAGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATCATT 1057 0.22087463849278657 No Hit TTCATAGGGGCCGACCCTTTCCCCATCTTAGCTGCCCTTGCCTGGCTTTCTC 1044 0.21815811029940319 No Hit TTCATAGGGGCCCTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACC 917 0.19161971948711948 No Hit TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 856 0.17887293334893595 No Hit TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 833 0.1740667680837192 No Hit TTCATAGGGATTCTGGGCTCTGCCTCCTGCCTACACCACCATAGCCAGTGCC 831 0.17364884066935254 No Hit TTCATAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 733 0.15317039736538557 No Hit TTCATAGGGGGGCTGGAGAGATGGCTCGGTGGTCAAGAGCACTCTCTGCTCT 715 0.1494090506360855 No Hit TTCATAGGGGCAAACCTGGCCACCAGGACCTTCCCTCTGACAAGAGGACACT 709 0.1481552683929855 No Hit TTCATAGGGCTCCACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTG 701 0.14648355873551883 No Hit TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 697 0.14564770390678547 No Hit TTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 664 0.13875190156973535 No Hit TTCATAGGGGTTCTGTGCCCTCCCTTTCAAAAGCTCTGAGCCCCGCTCATGC 660 0.13791604674100202 No Hit TTCATAGGGGTCGGGCGGTGGTGGCTCACACCTTTAATCCCAGCACTCGGGA 640 0.1337367725973353 No Hit TTCATAGGGGGGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGAG 614 0.12830371621056855 No Hit TTCATAGGGGAGTGCAGTGCTTTCATAGGGAAAAAAAAAAAAAAAAAAAAAA 589 0.12307962353098514 No Hit TTCATAGGGGTCCCCACCCACGGGGCCGTGGGGCTTTCCTGACTTGCACTGC 578 0.12078102275196845 No Hit TTCATAGGGAGGCTGGGCAGTGGTGGCACACGCCTTTAATCGCAGCACTCAG 574 0.11994516792323509 No Hit TTCATAGGGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTAGGG 571 0.11931827680168508 No Hit TTCATAGGGGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCT 539 0.11263143817181831 No Hit TTCATAGGGGGCAGAAGGGTCAAGGAAAGCCCTCAGCCTCACCTCCTCATGG 537 0.11221351075745163 No Hit TTCATAGGGCAGTGCTTTCATAGGGAGCCACTTGCCCCAATTTAAGTTTAGA 527 0.1101238736856183 No Hit TTCATAGGGGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATCTC 509 0.10636252695631822 No Hit TTCATAGGGGTTGATCTCAGCCCCTACTCCCAGACTGCTGAGGTAACTACAG 494 0.10322807134856818 No Hit TTCATAGGGATTTTGTTTTCTTGTAGGCCAGGCTGACTATTCACAGTATAGC 494 0.10322807134856818 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.030090773834400442 0.0 0.0 0.0 0.0 7 0.03071766495595045 0.0 0.0 0.0 0.0 8 0.034061084270883835 0.0 0.0 0.0 0.0 9 0.03782243100018389 0.0 0.0 0.0 0.0 10 0.05850983801133419 0.0 0.0 0.0 0.0 11 0.09591434159715141 0.0 0.0 0.0 0.0 12 0.11994516792323509 0.0 0.0 0.0 0.0 13 0.13478159113325197 0.0 0.0 0.0 0.0 14 0.15421521590130227 0.0 0.0 0.0 0.0 15 0.17072334876878584 0.0 0.0 0.0 0.0 16 0.1951721025092362 0.0 0.0 0.0 0.0 17 0.21063541684080309 0.0 0.0 0.0 0.0 18 0.22672562229392 0.0 0.0 0.0 0.0 19 0.24762199301225363 0.0 0.0 0.0 0.0 20 0.26454805329410386 0.0 0.0 0.0 0.0 21 0.2791755127969374 0.0 0.0 0.0 0.0 22 0.295056754542871 0.0 0.0 0.0 0.0 23 0.31908758086895467 0.0 0.0 0.0 0.0 24 0.341655661244755 0.0 0.0 0.0 0.0 25 0.3644327053277387 0.0 0.0 0.0 0.0 26 0.3790601648305722 0.0 0.0 0.0 0.0 27 0.4007923903776392 0.0 0.0 0.0 0.0 28 0.42398736187498953 0.0 0.0 0.0 0.0 29 0.44300305922867317 0.0 0.0 0.0 0.0 30 0.46201875658235675 0.0 0.0 0.0 0.0 31 0.47434761530617364 0.0 0.0 0.0 0.0 32 0.4927364215383072 0.0 0.0 0.0 0.0 33 0.5063190625052241 0.0 0.0 0.0 0.0 34 0.5251257961517244 0.0 0.0 0.0 0.0 35 0.544768384626958 0.0 0.0 0.0 0.0 36 0.5669185375883916 0.0 0.0 0.0 0.0 37 0.5840535615774253 0.0 0.0 0.0 0.0 38 0.5995168759089922 0.0 0.0 0.0 0.0 39 0.6222939199919758 0.0 0.0 0.0 0.0 40 0.642981327003126 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGGA 35 1.0178883E-7 46.000004 35 GCGTTAT 35 1.0178883E-7 46.000004 32 GTTCGTA 35 1.0178883E-7 46.000004 30 CACTACG 35 1.0178883E-7 46.000004 33 CCGCGTT 35 1.0178883E-7 46.000004 32 CGACTTG 35 1.0178883E-7 46.000004 29 CGACTAG 35 1.0178883E-7 46.000004 38 TCACGCT 25 3.4141456E-5 46.0 15 TCACGAC 40 5.5988494E-9 46.0 40 AACGTTA 20 6.3077436E-4 46.0 27 CGGCGTA 20 6.3077436E-4 46.0 31 CGTGGCG 40 5.5988494E-9 46.0 27 TAATAGC 30 1.8591109E-6 46.0 18 CCTTATA 40 5.5988494E-9 46.0 25 TCGCATC 20 6.3077436E-4 46.0 45 AGCCGAA 30 1.8591109E-6 46.0 10 AATCCCG 25 3.4141456E-5 46.0 36 CAAACCG 55 1.8189894E-12 46.0 10 GGTAATC 30 1.8591109E-6 46.0 31 GGTAACG 20 6.3077436E-4 46.0 9 >>END_MODULE