FastQCFastQC Report
Fri 17 Jun 2016
SRR1527421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634868
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC41850.6591921470289887No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC26910.42386763862724225No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT20530.32337430773011083No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA17690.2786405993056824No Hit
TTCATAGGGGCCGACCCTTTCCCCATCTTAGCTGCCCTTGCCTGGCTTTCTC13960.21988822873416206No Hit
TTCATAGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATCATT13950.21973071567632957No Hit
TTCATAGGGGCCCTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACC12000.18901566939899317No Hit
TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT11000.17326436361574374No Hit
TTCATAGGGCTCCACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTG10700.1685389718807689No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10680.16822394576510394No Hit
TTCATAGGGATTCTGGGCTCTGCCTCCTGCCTACACCACCATAGCCAGTGCC10420.16412860626145906No Hit
TTCATAGGGGCAAACCTGGCCACCAGGACCTTCCCTCTGACAAGAGGACACT9680.15247263998185448No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT9160.1442819609745648No Hit
TTCATAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8830.13908403006609246No Hit
TTCATAGGGGTTCTGTGCCCTCCCTTTCAAAAGCTCTGAGCCCCGCTCATGC8660.13640630808294008No Hit
TTCATAGGGAGGCTGGGCAGTGGTGGCACACGCCTTTAATCGCAGCACTCAG8420.1326259946949602No Hit
TTCATAGGGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTAGGG7850.12364775039850803No Hit
TTCATAGGGGTCGGGCGGTGGTGGCTCACACCTTTAATCCCAGCACTCGGGA7680.12097002841535563No Hit
TTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7600.11970992395269567No Hit
TTCATAGGGGGCAGAAGGGTCAAGGAAAGCCCTCAGCCTCACCTCCTCATGG7460.11750474114304076No Hit
TTCATAGGGGTCCCCACCCACGGGGCCGTGGGGCTTTCCTGACTTGCACTGC7450.11734722808520825No Hit
TTCATAGGGGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCT7430.11703220196954327No Hit
TTCATAGGGATTTTGTTTTCTTGTAGGCCAGGCTGACTATTCACAGTATAGC7400.11655966279604581No Hit
TTCATAGGGGGGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGAG7110.11199178411890347No Hit
TTCATAGGGGAGTGCAGTGCTTTCATAGGGAAAAAAAAAAAAAAAAAAAAAA6990.11010162742491353No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCGGTGGTCAAGAGCACTCTCTGCTCT6920.10899903602008605No Hit
TTCATAGGGCAGTGCTTTCATAGGGAGCCACTTGCCCCAATTTAAGTTTAGA6850.1078964446152586No Hit
TTCATAGGGGTGAAGGCAATGCCCTCAAAACTATTTTACTAAACTGAATTGC6750.10632131403693365No Hit
TTCATAGGGGTTGATCTCAGCCCCTACTCCCAGACTGCTGAGGTAACTACAG6600.10395861816944625No Hit
TTCATAGGGGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATCTC6410.10096587007062886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAACG551.8189894E-1246.0000049
GAATGCG551.8189894E-1246.00000429
AGCGCGT551.8189894E-1246.00000427
CGGTCGT551.8189894E-1246.00000427
CGGACAA301.8600767E-646.012
TCACGCC253.415416E-546.016
TCACGAA206.309309E-446.037
TCTGCGA206.309309E-446.018
CTTCGTA253.415416E-546.032
TCGCCGA206.309309E-446.013
ATTTACG206.309309E-446.014
AACGTTA206.309309E-446.027
GGAATCG405.6043064E-946.029
TAATAGC453.092282E-1046.018
ACTATGC501.6370905E-1146.029
AACCGAT206.309309E-446.031
CAAACCG453.092282E-1046.010
GATCGTT206.309309E-446.09
GGTCGTA253.415416E-546.09
GGTCGAC301.8600767E-646.09
Produced by FastQC (version 0.11.5)