##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527421_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	634868
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.021678522149486	31.0	31.0	33.0	30.0	34.0
2	31.165193394532405	31.0	31.0	34.0	30.0	34.0
3	31.948011240131805	33.0	31.0	34.0	30.0	34.0
4	34.803315019815145	35.0	35.0	37.0	32.0	37.0
5	35.541514141522335	37.0	35.0	37.0	35.0	37.0
6	34.760920380299524	35.0	35.0	37.0	32.0	37.0
7	36.036089391810584	37.0	35.0	37.0	35.0	37.0
8	36.5114307226069	37.0	37.0	37.0	35.0	37.0
9	38.62233724175734	39.0	39.0	39.0	38.0	39.0
10	37.00363067598304	39.0	37.0	39.0	33.0	39.0
11	37.06378491276927	39.0	37.0	39.0	34.0	39.0
12	37.06709268698375	39.0	37.0	39.0	34.0	39.0
13	37.03136715033676	39.0	37.0	39.0	33.0	39.0
14	37.799440198592464	40.0	38.0	40.0	33.0	40.0
15	37.88188253306199	40.0	38.0	40.0	33.0	40.0
16	37.95592784641847	40.0	38.0	40.0	34.0	40.0
17	37.961590440847544	40.0	38.0	40.0	34.0	40.0
18	37.82397758274161	40.0	37.0	40.0	33.0	40.0
19	37.71476432896287	40.0	37.0	40.0	33.0	40.0
20	37.66354895820864	40.0	37.0	40.0	33.0	40.0
21	37.70971603545934	40.0	37.0	40.0	33.0	40.0
22	37.75173264363616	40.0	37.0	40.0	33.0	40.0
23	37.737838416804756	40.0	37.0	40.0	34.0	40.0
24	37.73427074604485	40.0	37.0	40.0	34.0	40.0
25	37.686794735283556	39.0	37.0	40.0	34.0	40.0
26	37.60234095906551	39.0	37.0	40.0	34.0	40.0
27	37.5218958901693	39.0	36.0	40.0	33.0	40.0
28	37.37103145850791	39.0	36.0	40.0	33.0	40.0
29	37.361851597497434	39.0	36.0	40.0	33.0	40.0
30	37.287195133476565	39.0	36.0	40.0	33.0	40.0
31	37.22894207929837	39.0	36.0	40.0	33.0	40.0
32	37.149914627922655	39.0	36.0	40.0	33.0	40.0
33	37.077987865194025	39.0	35.0	40.0	33.0	40.0
34	36.80569031672726	39.0	35.0	40.0	31.0	40.0
35	36.77218256393455	39.0	35.0	40.0	31.0	40.0
36	36.713049011763076	39.0	35.0	40.0	31.0	40.0
37	36.7798818022014	39.0	35.0	40.0	32.0	40.0
38	36.696310099107215	39.0	35.0	40.0	32.0	40.0
39	36.55650465923625	39.0	35.0	40.0	31.0	40.0
40	36.712488265277194	39.0	35.0	40.0	32.0	40.0
41	36.760039882306245	39.0	35.0	40.0	32.0	40.0
42	36.809103624690486	39.0	35.0	40.0	33.0	40.0
43	36.76173787306968	39.0	35.0	40.0	32.0	40.0
44	36.69372058443645	39.0	35.0	40.0	32.0	40.0
45	36.60016727886742	39.0	35.0	40.0	32.0	40.0
46	36.39398426129526	39.0	35.0	40.0	31.0	40.0
47	36.34182066193287	39.0	35.0	40.0	31.0	40.0
48	36.279380910677496	39.0	35.0	40.0	31.0	40.0
49	36.204963866504535	39.0	35.0	40.0	31.0	40.0
50	36.04344840187252	38.0	35.0	40.0	31.0	40.0
51	35.90739807330028	38.0	35.0	40.0	31.0	40.0
52	35.47126016746788	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	6.0
15	10.0
16	33.0
17	74.0
18	115.0
19	198.0
20	326.0
21	503.0
22	803.0
23	1191.0
24	1774.0
25	2572.0
26	3332.0
27	4753.0
28	5865.0
29	7429.0
30	9226.0
31	12133.0
32	16480.0
33	24613.0
34	42908.0
35	41163.0
36	64439.0
37	113043.0
38	234593.0
39	47282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.63866504533226	0.2828934518671598	0.044261169250930904	0.03418033354965127
8	99.15714762753832	0.7393662934657284	0.0774964244535872	0.025989654542361563
9	97.85955505711424	1.3087759975301954	0.2542260753416458	0.5774428700139241
10	48.369739851433685	41.25692269889174	3.562157802881859	6.811179646792718
11	32.71530459875124	23.342490092428662	27.465079355078537	16.477125953741563
12	30.953206020779124	20.031880642905296	25.311088289219175	23.703825047096405
13	24.81507967010465	20.254604106680443	29.19992817404563	25.730388049169278
14	26.106056692099777	22.26573082908573	29.08132084149776	22.546891637316733
15	25.845845120560497	22.30479406742819	28.585627248498902	23.263733563512414
16	28.763774516907453	24.587315788478865	25.78803782833597	20.860871866277712
17	29.322788359154973	23.527252909266178	24.348210966689138	22.80174776488971
18	26.397770875205556	23.584430149259376	27.627475317703837	22.390323657831235
19	26.50739996345697	24.563373803688325	27.018529836123417	21.91069639673129
20	25.047568943465414	25.998632786658014	26.4643988986687	22.489399371207874
21	24.823742888285437	23.90622932641116	30.422702041999283	20.847325743304122
22	26.202612196551094	23.61640529999937	27.96360818311838	22.217374320331153
23	25.23485196922825	24.355141541233767	28.72833407889514	21.68167241064284
24	25.648953798269876	24.150847105225022	26.965290422576032	23.23490867392907
25	24.62496140930083	27.956992634689414	24.773653735894705	22.644392220115048
26	25.25091830112716	25.434578526559854	25.408903898133158	23.905599274179828
27	24.004990013672135	25.821115570480792	24.628111670457482	25.545782745389594
28	25.47742207829029	26.327677564470093	25.37771631268232	22.817184044557294
29	27.2031351399031	26.26624747191542	24.76451797854042	21.76609940964106
30	27.46082650251706	24.783104519364656	24.772551144489878	22.983517833628408
31	27.280001512125356	25.23028409055111	25.021106749749556	22.468607647573986
32	27.694733393398312	26.55591398526938	24.10138800506562	21.647964616266687
33	27.815388395698005	23.74021056345571	26.46833672511451	21.976064315731776
34	24.837289011259035	25.938305285508168	27.74781529388786	21.476590409344933
35	24.447601706181445	28.942236811431666	25.07529124164393	21.534870240742958
36	23.785259297995804	29.429897238481068	25.16680632824461	21.618037135278513
37	25.18303017320136	28.515691450821272	24.830515949772234	21.47076242620513
38	25.04867153487024	28.958145630272746	24.569201786828128	21.42398104802888
39	25.271237485587555	27.99385069022222	23.35871393738541	23.376197886804817
40	25.212957654189534	27.77979044462786	23.166232980714103	23.841018920468507
41	22.633051279951108	27.8856392194913	23.929856285086036	25.551453215471565
42	21.797129482034062	27.91950452692528	24.70702571243156	25.576340278609095
43	22.71417050473484	25.964767479224026	26.25663917538764	25.06442284065349
44	24.25228551446915	25.695105124214795	25.062060144786003	24.99054921653005
45	24.557230794432858	28.108362683266442	23.811091439480332	23.523315082820364
46	23.1536319360875	28.451111097109948	23.53119073571199	24.864066231090558
47	23.454481876547568	27.18832891246684	26.100386222017807	23.256802988967785
48	22.909486696447136	27.476892834415974	26.60017515452031	23.013445314616582
49	25.8192254137868	25.018271514708566	25.44513190143463	23.71737117007
50	23.002419400568307	27.567305329611823	25.235324508401746	24.19495076141812
51	23.08779147791352	27.228652255271964	26.32310968579295	23.360446581021566
52	23.795970185928415	27.274646068159054	24.721989452925648	24.20739429298689
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	3.0
2	6.0
3	20.0
4	34.0
5	42.5
6	51.0
7	573.0
8	1095.0
9	1697.0
10	2299.0
11	2683.0
12	3067.0
13	2592.5
14	1820.5
15	1523.0
16	1885.0
17	2247.0
18	2217.0
19	2187.0
20	3035.0
21	3883.0
22	4951.5
23	6020.0
24	6659.5
25	7299.0
26	7807.0
27	8315.0
28	10198.5
29	12082.0
30	12356.0
31	12630.0
32	13019.5
33	13409.0
34	15725.5
35	18042.0
36	18668.0
37	19294.0
38	21160.0
39	26232.5
40	29439.0
41	29992.5
42	30546.0
43	30864.5
44	31183.0
45	33646.5
46	36110.0
47	37368.0
48	38626.0
49	40663.0
50	42700.0
51	44622.0
52	46544.0
53	46271.5
54	45999.0
55	45675.0
56	45351.0
57	43133.0
58	40915.0
59	40228.0
60	39541.0
61	33313.5
62	27086.0
63	21547.5
64	13941.0
65	11873.0
66	8672.5
67	5472.0
68	4828.0
69	4184.0
70	3305.5
71	2427.0
72	1750.5
73	1074.0
74	758.5
75	443.0
76	356.5
77	270.0
78	347.0
79	424.0
80	221.0
81	18.0
82	11.5
83	5.0
84	3.0
85	1.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	634868.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.39764486475929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92233278054174	8.197452068776503
2	9.165284687672749	2.0892531990902046
3	3.522664455500277	1.204502353245084
4	1.7177998894416804	0.7831549235431617
5	0.9286898839137645	0.5292438743171809
6	0.642620232172471	0.43946143135265914
7	0.5362078496406855	0.42780546507305456
8	0.41735765616362636	0.3805515477233063
9	0.3676064123825318	0.37708626045099136
>10	6.48424543946932	19.636995406919233
>50	2.5925925925925926	20.59546236382996
>100	1.6362631288004421	38.52974161557993
>500	0.051133222775013816	3.8214242960741447
>1k	0.015201768933112217	2.9878651940245846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4185	0.6591921470289887	No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2691	0.42386763862724225	No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2053	0.32337430773011083	No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1769	0.2786405993056824	No Hit
TTCATAGGGGCCGACCCTTTCCCCATCTTAGCTGCCCTTGCCTGGCTTTCTC	1396	0.21988822873416206	No Hit
TTCATAGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATCATT	1395	0.21973071567632957	No Hit
TTCATAGGGGCCCTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACC	1200	0.18901566939899317	No Hit
TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1100	0.17326436361574374	No Hit
TTCATAGGGCTCCACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTG	1070	0.1685389718807689	No Hit
TTCATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1068	0.16822394576510394	No Hit
TTCATAGGGATTCTGGGCTCTGCCTCCTGCCTACACCACCATAGCCAGTGCC	1042	0.16412860626145906	No Hit
TTCATAGGGGCAAACCTGGCCACCAGGACCTTCCCTCTGACAAGAGGACACT	968	0.15247263998185448	No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	916	0.1442819609745648	No Hit
TTCATAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	883	0.13908403006609246	No Hit
TTCATAGGGGTTCTGTGCCCTCCCTTTCAAAAGCTCTGAGCCCCGCTCATGC	866	0.13640630808294008	No Hit
TTCATAGGGAGGCTGGGCAGTGGTGGCACACGCCTTTAATCGCAGCACTCAG	842	0.1326259946949602	No Hit
TTCATAGGGGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTAGGG	785	0.12364775039850803	No Hit
TTCATAGGGGTCGGGCGGTGGTGGCTCACACCTTTAATCCCAGCACTCGGGA	768	0.12097002841535563	No Hit
TTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	760	0.11970992395269567	No Hit
TTCATAGGGGGCAGAAGGGTCAAGGAAAGCCCTCAGCCTCACCTCCTCATGG	746	0.11750474114304076	No Hit
TTCATAGGGGTCCCCACCCACGGGGCCGTGGGGCTTTCCTGACTTGCACTGC	745	0.11734722808520825	No Hit
TTCATAGGGGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCT	743	0.11703220196954327	No Hit
TTCATAGGGATTTTGTTTTCTTGTAGGCCAGGCTGACTATTCACAGTATAGC	740	0.11655966279604581	No Hit
TTCATAGGGGGGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGAG	711	0.11199178411890347	No Hit
TTCATAGGGGAGTGCAGTGCTTTCATAGGGAAAAAAAAAAAAAAAAAAAAAA	699	0.11010162742491353	No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCGGTGGTCAAGAGCACTCTCTGCTCT	692	0.10899903602008605	No Hit
TTCATAGGGCAGTGCTTTCATAGGGAGCCACTTGCCCCAATTTAAGTTTAGA	685	0.1078964446152586	No Hit
TTCATAGGGGTGAAGGCAATGCCCTCAAAACTATTTTACTAAACTGAATTGC	675	0.10632131403693365	No Hit
TTCATAGGGGTTGATCTCAGCCCCTACTCCCAGACTGCTGAGGTAACTACAG	660	0.10395861816944625	No Hit
TTCATAGGGGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATCTC	641	0.10096587007062886	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.024257010906204125	0.0	0.0	0.0	0.0
7	0.024572037021869112	0.0	0.0	0.0	0.0
8	0.026147167600194057	0.0	0.0	0.0	0.0
9	0.027564785120686504	0.0	0.0	0.0	0.0
10	0.04457619536659589	0.0	0.0	0.0	0.0
11	0.07670885916442473	0.0	0.0	0.0	0.0
12	0.09466534775732909	0.0	0.0	0.0	0.0
13	0.1080539576730911	0.0	0.0	0.0	0.0
14	0.12601044626599545	0.0	0.0	0.0	0.0
15	0.13955656923958995	0.0	0.0	0.0	0.0
16	0.15940321452648423	0.0	0.0	0.0	0.0
17	0.17216177221091628	0.0	0.0	0.0	0.0
18	0.18429027766401834	0.0	0.0	0.0	0.0
19	0.20035660956293277	0.0	0.0	0.0	0.0
20	0.21390273253652728	0.0	0.0	0.0	0.0
21	0.22603123798962935	0.0	0.0	0.0	0.0
22	0.24209756988854375	0.0	0.0	0.0	0.0
23	0.2606841107127781	0.0	0.0	0.0	0.0
24	0.27659292955386	0.0	0.0	0.0	0.0
25	0.29439190508893187	0.0	0.0	0.0	0.0
26	0.3104582369878463	0.0	0.0	0.0	0.0
27	0.32857223863858315	0.0	0.0	0.0	0.0
28	0.35267173648695477	0.0	0.0	0.0	0.0
29	0.3715733034268541	0.0	0.0	0.0	0.0
30	0.38874222673059594	0.0	0.0	0.0	0.0
31	0.4038634802825154	0.0	0.0	0.0	0.0
32	0.42008732523926234	0.0	0.0	0.0	0.0
33	0.4341059873863543	0.0	0.0	0.0	0.0
34	0.45379511961541613	0.0	0.0	0.0	0.0
35	0.4771070521746253	0.0	0.0	0.0	0.0
36	0.498371314982012	0.0	0.0	0.0	0.0
37	0.5175879080375763	0.0	0.0	0.0	0.0
38	0.5355443966304807	0.0	0.0	0.0	0.0
39	0.5538159113390501	0.0	0.0	0.0	0.0
40	0.5783879483609191	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAACG	55	1.8189894E-12	46.000004	9
GAATGCG	55	1.8189894E-12	46.000004	29
AGCGCGT	55	1.8189894E-12	46.000004	27
CGGTCGT	55	1.8189894E-12	46.000004	27
CGGACAA	30	1.8600767E-6	46.0	12
TCACGCC	25	3.415416E-5	46.0	16
TCACGAA	20	6.309309E-4	46.0	37
TCTGCGA	20	6.309309E-4	46.0	18
CTTCGTA	25	3.415416E-5	46.0	32
TCGCCGA	20	6.309309E-4	46.0	13
ATTTACG	20	6.309309E-4	46.0	14
AACGTTA	20	6.309309E-4	46.0	27
GGAATCG	40	5.6043064E-9	46.0	29
TAATAGC	45	3.092282E-10	46.0	18
ACTATGC	50	1.6370905E-11	46.0	29
AACCGAT	20	6.309309E-4	46.0	31
CAAACCG	45	3.092282E-10	46.0	10
GATCGTT	20	6.309309E-4	46.0	9
GGTCGTA	25	3.415416E-5	46.0	9
GGTCGAC	30	1.8600767E-6	46.0	9
>>END_MODULE