FastQCFastQC Report
Fri 17 Jun 2016
SRR1527420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492728
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC699162.8047985981623347No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG606322.4323552349072983No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC260531.0451601618788733No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT214360.8599413975371561No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT213510.8565314787654328No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT100860.4046169497835303No Hit
CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT95900.38471907083323975No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC76910.3085374738038005No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC70460.28266220783013635No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGA65130.2612800112968603No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC64680.2594747601824186No Hit
CTCCATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTG59460.23853384725489504No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA57910.23231576008292923No Hit
CTCCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA57130.22918665815123032No Hit
CTCCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG54490.2185958516131724No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC54330.2179539845502598No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTAGGTAAGAGCACCCGACT53570.21490511600142495No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG47510.19059440099361022No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG44990.18048499475273677No Hit
CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA44400.17811810995824656No Hit
CTCCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT43380.17402620743217873No Hit
CTCCATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC41520.16656450282581975No Hit
CTCCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC41010.16451855156278583No Hit
CTCCATGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT40370.16195108331113542No Hit
CTCCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC38200.15324576127038328No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37300.1496352590414999No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA36920.14811082476708248No Hit
CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG32050.12857399603968023No Hit
CTCCATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG31530.12648792808521428No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC30600.12275707578203478No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG30390.121914625261962No Hit
CTCCATGGGGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCGACTGC30340.1217140418048018No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG29330.11766225597016602No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT28420.11401163704985061No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG28100.1127279029240254No Hit
CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT28010.11236685270113707No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTG26880.10783366656931684No Hit
CTCCATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA26300.10550689846625864No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA25660.10293943021460826No Hit
CTCCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAA25450.10209697969453546No Hit
CTCCATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA25110.1007330121858462No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG207.8582537E-444.00000418
TACGTTT207.8582537E-444.00000413
CGTTGAC351.4469879E-744.038
CCGTATT254.4443434E-544.016
ATGGGGT172650.041.9739345
ATGGGGC349200.041.9650655
CATGGGG1823750.041.8322684
TCCATGG2599850.041.7880252
ATGGGAT102850.041.7754025
CCATGGG2572000.041.757233
ATGGGGG928400.041.7369655
CTCCATG2614000.041.724251
ATGGGAG195150.041.6889575
TGGGGGG588600.041.3088656
ATGGGAC80550.041.2687765
CATGGGT92150.041.2544754
ATGGGGA379750.041.1902585
CATGGGA496550.041.1511424
ATGGGAA114350.040.9024935
CATGGGC168250.040.8487364
Produced by FastQC (version 0.11.5)