FastQCFastQC Report
Fri 17 Jun 2016
SRR1527419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211095
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC598292.705853886875055No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT440191.9908235512268808No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA256011.157842607395883No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC188750.8536494361391076No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC160630.7264726300769528No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT104100.4708074506070522No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG95090.43005840997333894No Hit
CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT83270.3766007340254489No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT69700.3152284275438188No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC63920.28908753355238015No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC59470.26896175876658396No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG56480.2554390471689367No Hit
CTCCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC55250.2498761925652222No Hit
CTCCATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT54670.24725305787403978No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG54550.24671034035172618No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC47380.2142829683934883No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTAGGTAAGAGCACCCGACTGC46500.21030303989652185No Hit
CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA41090.18583552493221686No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC40190.1817651435148648No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT39930.180589255549852No Hit
CTCCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT38890.17588570368980075No Hit
CTCCATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT37580.1699610374045439No Hit
CTCCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT37020.16742835563374708No Hit
CTCCATGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC36030.16295093607465982No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC32880.14870460111392772No Hit
CTCCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC31470.14232767022674286No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC30370.1373527596055348No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT30160.136403003941486No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC29940.1354080218172444No Hit
CTCCATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA29520.13350851048914678No Hit
CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT28990.13111150809892835No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT28500.12889541154948114No Hit
CTCCATGGGGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCGACTGCTC28330.12812656172620354No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC28030.12676976792041952No Hit
CTCCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT27860.12600091809714192No Hit
CTCCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG27020.12220189544094669No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT25980.11749834358089543No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG25150.11374454738489301No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG25040.11324705632277221No Hit
CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT25020.1131566034023866No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT24910.11265911234026581No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT23970.10840782508214256No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT23960.10836259862194975No Hit
CTCCATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG23780.10754852233847935No Hit
CTCCATGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCT23330.10551333162980334No Hit
CTCCATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG23070.10433744366479053No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG22640.10239270587650011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT253.418188E-546.046
CGTATCG301.8621886E-646.033
CTCGTAC206.312725E-446.029
CGCATTA206.312725E-446.018
CGTACTT206.312725E-446.041
CGTAATA301.8621886E-646.023
CGTAACG301.8621886E-646.045
CGGTAAT253.418188E-546.040
GCCGTAT301.8621886E-646.031
GCGTAAT253.418188E-546.022
ATGGGGT151750.044.0751235
ATGGGGC308000.043.946435
CATGGGG1617800.043.7565844
TCCATGG2304200.043.7091872
ATGGGGG820700.043.7047655
CTCCATG2313100.043.704081
ATGGGAT91450.043.661025
CCATGGG2281400.043.641933
ATGGGAG177200.043.417045
CATGGGT75700.043.356674
Produced by FastQC (version 0.11.5)