##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527419_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2211095
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21741942340786	33.0	31.0	34.0	31.0	34.0
2	32.41664288508635	34.0	31.0	34.0	31.0	34.0
3	32.903381356296315	34.0	33.0	34.0	31.0	34.0
4	36.48580047442557	37.0	37.0	37.0	35.0	37.0
5	35.462991866021135	37.0	35.0	37.0	33.0	37.0
6	36.19805661900551	37.0	36.0	37.0	35.0	37.0
7	36.54552201511016	37.0	37.0	37.0	35.0	37.0
8	36.658379671610675	37.0	37.0	37.0	35.0	37.0
9	38.70622836196545	39.0	39.0	39.0	39.0	39.0
10	38.00946996849977	39.0	39.0	39.0	35.0	39.0
11	37.86644445399225	39.0	37.0	39.0	35.0	39.0
12	37.059377367322526	39.0	35.0	39.0	35.0	39.0
13	36.98787659508072	39.0	35.0	39.0	34.0	39.0
14	37.49676472517011	40.0	35.0	40.0	33.0	40.0
15	37.63577548680631	40.0	35.0	40.0	34.0	40.0
16	37.74919169009021	40.0	35.0	40.0	34.0	40.0
17	37.56301380085433	40.0	35.0	40.0	33.0	40.0
18	37.46585967586196	40.0	35.0	40.0	33.0	40.0
19	37.280457872682994	40.0	35.0	40.0	33.0	40.0
20	37.18859479126858	39.0	35.0	40.0	32.0	40.0
21	37.18131061758993	39.0	35.0	40.0	32.0	40.0
22	37.24488500041834	39.0	35.0	40.0	33.0	40.0
23	37.21184028727848	39.0	35.0	40.0	33.0	40.0
24	37.2521234953722	39.0	35.0	40.0	34.0	40.0
25	37.30043259109174	39.0	35.0	40.0	34.0	40.0
26	37.23484201266793	39.0	35.0	40.0	34.0	40.0
27	37.19013068185673	39.0	35.0	40.0	34.0	40.0
28	37.010457262125776	39.0	35.0	40.0	33.0	40.0
29	36.912529764664114	39.0	35.0	40.0	33.0	40.0
30	36.71744497635787	39.0	35.0	40.0	32.0	40.0
31	36.675610500679525	39.0	35.0	40.0	32.0	40.0
32	36.569937971909845	38.0	35.0	40.0	32.0	40.0
33	36.47875600098594	38.0	35.0	40.0	32.0	40.0
34	35.79070370110737	38.0	34.0	40.0	30.0	40.0
35	35.66268342156262	38.0	34.0	40.0	29.0	40.0
36	35.81069198745418	38.0	34.0	40.0	30.0	40.0
37	35.796495853864265	38.0	35.0	40.0	30.0	40.0
38	35.96367094132093	38.0	35.0	40.0	31.0	40.0
39	35.92557488484213	38.0	35.0	40.0	31.0	40.0
40	35.70457669163921	37.0	35.0	40.0	30.0	40.0
41	35.58835147291274	37.0	35.0	40.0	30.0	40.0
42	35.539009404842396	37.0	35.0	40.0	30.0	40.0
43	35.50566303121304	36.0	35.0	40.0	30.0	40.0
44	35.419100490933225	36.0	35.0	40.0	30.0	40.0
45	35.254823967310315	36.0	34.0	40.0	30.0	40.0
46	35.02930131902971	35.0	34.0	40.0	29.0	40.0
47	34.8020645879078	35.0	33.0	40.0	28.0	40.0
48	34.66039903305828	35.0	33.0	39.0	28.0	40.0
49	34.62175844999876	35.0	33.0	39.0	28.0	40.0
50	34.97909542556968	35.0	34.0	39.0	30.0	40.0
51	35.074078228208194	35.0	34.0	39.0	30.0	40.0
52	34.809526953839615	35.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	11.0
13	33.0
14	54.0
15	80.0
16	166.0
17	332.0
18	558.0
19	938.0
20	1404.0
21	2110.0
22	3222.0
23	4718.0
24	6789.0
25	9077.0
26	12001.0
27	15915.0
28	20143.0
29	25839.0
30	34496.0
31	46712.0
32	69493.0
33	106909.0
34	279432.0
35	213471.0
36	198992.0
37	331484.0
38	644033.0
39	182676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.46650867556572	0.35715335614254473	0.09886504198146166	0.07747292631026709
8	99.45823223335044	0.2659768123938591	0.17412187174228155	0.1016690825134153
9	98.59888426322705	0.7394526241522865	0.2145091006944523	0.4471540119262175
10	70.83454125670765	18.84012220189544	3.5595033230141624	6.765833218382747
11	46.0072498015689	21.953014230505698	13.759245984455665	18.280489983469728
12	40.65397461438789	14.581598710141355	21.823304742672747	22.941121932798005
13	29.434601407899706	12.800354575447912	23.71987635085783	34.04516766579455
14	23.721323597584	14.966475886382085	32.36930118335033	28.94289933268358
15	28.990884606948143	14.059414000755282	27.7253577978332	29.224343594463377
16	36.981269461511154	15.273246965869852	23.349245509577834	24.39623806304116
17	36.17112788007752	18.58395953136342	23.039851295398886	22.205061293160178
18	36.07664980473476	17.746229809212178	23.71657481926376	22.460545566789307
19	35.06633591048779	22.808517951512712	19.286462137538187	22.83868400046131
20	36.87602748864251	24.649777598882004	17.86033616827861	20.613858744196882
21	35.0191647125067	23.960435892623337	21.78232956973807	19.23806982513189
22	31.083241561307855	20.28275582912539	26.090692620624623	22.54330998894213
23	33.30435824783648	19.594544784371546	24.790341437161224	22.31075553063075
24	37.1248182461631	19.445116559894533	20.3536709187077	23.07639427523467
25	32.95163708479283	16.48269296434572	19.501830540976304	31.063839409885148
26	26.691390464905396	16.62009095041145	25.14763047268435	31.540888111998804
27	23.66877045083997	16.361983542091135	25.154323988792882	34.814922018276015
28	26.056998907780986	24.350242753025082	19.497172215576445	30.095586123617483
29	32.87592799043008	23.307727619120843	17.993573320006604	25.822771070442474
30	32.29815996146706	19.350005314109072	23.87970666117919	24.472128063244682
31	36.901489985731054	17.384463354129966	22.188417955809225	23.525628704329755
32	41.610604700386006	16.62284976448321	20.33802256348099	21.428522971649794
33	42.66732094279079	16.92681680343902	19.624349021638597	20.78151323213159
34	32.78149514154751	19.349100784905215	27.592482457786755	20.276921615760518
35	28.132260260187824	27.751272559523677	25.469281057575543	18.647186122712956
36	28.63639961195697	31.135478122830545	19.735244302031347	20.49287796318114
37	32.59118219705621	28.41415678656955	18.030704243824893	20.96395677254935
38	34.340813036074884	27.426410895958792	17.02161146400313	21.211164603963194
39	34.369712744138084	23.198912755896963	17.564148080475963	24.867226419488986
40	31.80369907217917	20.961243184937782	18.268912009660372	28.96614573322268
41	25.973646541645657	23.864420117634022	18.93536912706148	31.226564213658843
42	23.761575147155593	21.96757715068778	21.506448162561988	32.764399539594635
43	24.168206250749062	18.07859906516907	22.060834111605335	35.69236057247653
44	26.201633127477564	16.240550496473467	21.100585908791796	36.457230467257176
45	30.618358776986064	19.151099342181137	20.002623134691184	30.22791874614162
46	28.675972764625673	23.33092879319975	21.010766158848895	26.982332283325682
47	26.565389546808255	22.44815351669648	22.296283063369053	28.690173873126213
48	26.860356520185704	20.64651224845608	26.711380560310616	25.781750671047604
49	31.83549327369471	17.573690863576644	25.097202969569377	25.493612893159273
50	27.371279840983764	19.1117523218134	23.705222977755366	29.81174485944747
51	24.538203921586362	20.562164900196507	25.976043544035875	28.923587634181253
52	24.91055336835369	18.525255586033165	26.361689570099884	30.202501475513266
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	8.5
8	15.0
9	29.0
10	43.0
11	166.0
12	289.0
13	496.5
14	1077.0
15	1450.0
16	1518.0
17	1586.0
18	1661.5
19	1737.0
20	1768.5
21	1800.0
22	1774.0
23	1748.0
24	2069.0
25	2390.0
26	3056.5
27	3723.0
28	4582.5
29	5442.0
30	6708.5
31	7975.0
32	8930.0
33	9885.0
34	10659.5
35	11434.0
36	15483.0
37	19532.0
38	19322.0
39	21587.5
40	24063.0
41	25498.5
42	26934.0
43	29498.5
44	32063.0
45	36944.5
46	41826.0
47	49432.0
48	57038.0
49	58378.5
50	59719.0
51	65082.5
52	70446.0
53	83988.0
54	97530.0
55	110452.5
56	123375.0
57	158162.0
58	192949.0
59	206091.0
60	219233.0
61	240641.0
62	262049.0
63	291436.5
64	255688.5
65	190553.0
66	158211.5
67	125870.0
68	102399.5
69	78929.0
70	72034.5
71	65140.0
72	56872.0
73	48604.0
74	46437.0
75	44270.0
76	34392.5
77	24515.0
78	17175.0
79	9835.0
80	6695.5
81	3556.0
82	2691.0
83	1826.0
84	1269.0
85	712.0
86	499.0
87	286.0
88	172.0
89	31.5
90	5.0
91	7.5
92	10.0
93	10.0
94	10.0
95	5.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2211095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.44694144178908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.96113429502016	5.656167802658099
2	10.17753305411166	1.7193805145990795
3	3.760537809907491	0.9529512800976702
4	1.746489808682693	0.590099885704965
5	1.0235590838998074	0.4322971821956475
6	0.7029416966315278	0.3562624409063029
7	0.5504922374875528	0.3254982985951765
8	0.45161224634625574	0.3051793759425318
9	0.4191870484562919	0.318676360632003
>10	9.70020288248902	21.849695632239005
>50	2.7408846357925842	15.833881070136316
>100	1.6074320478627286	26.077190087923096
>500	0.0905899309094057	5.284671108015771
>1k	0.05931483571449183	9.638111601646738
>5k	0.004853032013003878	2.6982686033825267
>10k+	0.003235354675335918	7.961668755325042
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	59829	2.705853886875055	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	44019	1.9908235512268808	No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	25601	1.157842607395883	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	18875	0.8536494361391076	No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	16063	0.7264726300769528	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	10410	0.4708074506070522	No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9509	0.43005840997333894	No Hit
CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	8327	0.3766007340254489	No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6970	0.3152284275438188	No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6392	0.28908753355238015	No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5947	0.26896175876658396	No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	5648	0.2554390471689367	No Hit
CTCCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	5525	0.2498761925652222	No Hit
CTCCATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT	5467	0.24725305787403978	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG	5455	0.24671034035172618	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	4738	0.2142829683934883	No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTAGGTAAGAGCACCCGACTGC	4650	0.21030303989652185	No Hit
CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4109	0.18583552493221686	No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	4019	0.1817651435148648	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	3993	0.180589255549852	No Hit
CTCCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	3889	0.17588570368980075	No Hit
CTCCATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	3758	0.1699610374045439	No Hit
CTCCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	3702	0.16742835563374708	No Hit
CTCCATGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3603	0.16295093607465982	No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3288	0.14870460111392772	No Hit
CTCCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	3147	0.14232767022674286	No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3037	0.1373527596055348	No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	3016	0.136403003941486	No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2994	0.1354080218172444	No Hit
CTCCATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2952	0.13350851048914678	No Hit
CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	2899	0.13111150809892835	No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2850	0.12889541154948114	No Hit
CTCCATGGGGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2833	0.12812656172620354	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	2803	0.12676976792041952	No Hit
CTCCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2786	0.12600091809714192	No Hit
CTCCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG	2702	0.12220189544094669	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	2598	0.11749834358089543	No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2515	0.11374454738489301	No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2504	0.11324705632277221	No Hit
CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	2502	0.1131566034023866	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2491	0.11265911234026581	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	2397	0.10840782508214256	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT	2396	0.10836259862194975	No Hit
CTCCATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2378	0.10754852233847935	No Hit
CTCCATGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCT	2333	0.10551333162980334	No Hit
CTCCATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2307	0.10433744366479053	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG	2264	0.10239270587650011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.974910621208043E-4	0.0	0.0	0.0	0.0
8	7.236233630848064E-4	0.0	0.0	0.0	0.0
9	0.0032110786736888283	0.0	0.0	0.0	0.0
10	0.012889541154948114	0.0	0.0	0.0	0.0
11	0.04328172240450998	0.0	0.0	0.0	0.0
12	0.052779279044998066	0.0	0.0	0.0	0.0
13	0.05987983329526773	0.0	0.0	0.0	0.0
14	0.0784679084345087	0.0	0.0	0.0	0.0
15	0.08674435064979116	0.0	0.0	0.0	0.0
16	0.09660371897182166	0.0	0.0	0.0	0.0
17	0.10542287870941773	0.0	0.0	0.0	0.0
18	0.11175458313640979	0.0	0.0	0.0	0.0
19	0.1249607095127075	0.0	0.0	0.0	0.0
20	0.13717185376476362	0.0	0.0	0.0	0.0
21	0.1458101076615885	0.0	0.0	0.0	0.0
22	0.15218703854877336	0.0	0.0	0.0	0.0
23	0.16078006598540542	0.0	0.0	0.0	0.0
24	0.1719962281132199	0.0	0.0	0.0	0.0
25	0.17868974422175438	0.0	0.0	0.0	0.0
26	0.18787071564089286	0.0	0.0	0.0	0.0
27	0.19877029254735776	0.0	0.0	0.0	0.0
28	0.25566517946990064	0.0	0.0	0.0	0.0
29	0.26697179451810077	0.0	0.0	0.0	0.0
30	0.28949457169411535	0.0	0.0	0.0	0.0
31	0.30021324275980904	0.0	0.0	0.0	0.0
32	0.30989170524106835	0.0	0.0	0.0	0.0
33	0.3182586003767364	0.0	0.0	0.0	0.0
34	0.3285250068405021	0.0	0.0	0.0	0.0
35	0.3715805969440481	0.0	0.0	0.0	0.0
36	0.39102797482695223	0.0	0.0	0.0	0.0
37	0.4066311035934684	0.0	0.0	0.0	0.0
38	0.4180281715620541	0.0	0.0	0.0	0.0
39	0.42662119899868617	0.0	0.0	0.0	0.0
40	0.43521422643531826	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	25	3.418188E-5	46.0	46
CGTATCG	30	1.8621886E-6	46.0	33
CTCGTAC	20	6.312725E-4	46.0	29
CGCATTA	20	6.312725E-4	46.0	18
CGTACTT	20	6.312725E-4	46.0	41
CGTAATA	30	1.8621886E-6	46.0	23
CGTAACG	30	1.8621886E-6	46.0	45
CGGTAAT	25	3.418188E-5	46.0	40
GCCGTAT	30	1.8621886E-6	46.0	31
GCGTAAT	25	3.418188E-5	46.0	22
ATGGGGT	15175	0.0	44.075123	5
ATGGGGC	30800	0.0	43.94643	5
CATGGGG	161780	0.0	43.756584	4
TCCATGG	230420	0.0	43.709187	2
ATGGGGG	82070	0.0	43.704765	5
CTCCATG	231310	0.0	43.70408	1
ATGGGAT	9145	0.0	43.66102	5
CCATGGG	228140	0.0	43.64193	3
ATGGGAG	17720	0.0	43.41704	5
CATGGGT	7570	0.0	43.35667	4
>>END_MODULE