FastQCFastQC Report
Fri 17 Jun 2016
SRR1527418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771152
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC748932.702594444476521No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT560132.0212893410393944No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA300431.0841339630594062No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC230680.8324335871868451No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC191630.6915174627736046No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT127430.45984485874466646No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG120650.43537849962759173No Hit
CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT108680.39218346738107474No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT87830.3169440001847607No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC82800.2987927042616212No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG79680.2875338487387195No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC76950.27768235015618054No Hit
CTCCATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT68850.24845262908710888No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG66350.23943111023863No Hit
CTCCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC63950.23077045214409025No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC60030.21662471058967533No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTAGGTAAGAGCACCCGACTGC59480.21463997644300997No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC55100.19883427542047497No Hit
CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA54330.19605564761514346No Hit
CTCCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT51060.18425550096133306No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT50290.18147687315600156No Hit
CTCCATGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC46560.16801676703407104No Hit
CTCCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT45970.16588768858583No Hit
CTCCATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT45590.1645164177208612No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC43410.15664965328498762No Hit
CTCCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC39100.14109655479021No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT37610.13571972955651657No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC37370.1348536637470626No Hit
CTCCATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA36140.13041507647361097No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC35650.12864685877930912No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT35100.12666212463264376No Hit
CTCCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT34710.12525476769228105No Hit
CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT34500.12449696010900882No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT34020.12276482849010087No Hit
CTCCATGGGGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCGACTGCTC33120.11951708170464846No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC33070.11933665132767889No Hit
CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT32850.11854275766901275No Hit
CTCCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG31930.11522283873277252No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG31330.11305767420913757No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT30850.11132554259022961No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG30560.11027904640380608No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT30140.10876343123726162No Hit
CTCCATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG29130.10511873762247614No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG29000.10464961864235524No Hit
CTCCATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG28620.10327834777738645No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT28600.10320617562659862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA253.4184133E-546.033
ATATGCG206.313003E-446.031
CGAATTA253.4184133E-546.024
GACGAAT206.313003E-446.09
CGAAATA351.02027116E-745.99999610
ATGGGGC388800.043.9236155
ATGGGAG213550.043.7382355
ATGGGGT193900.043.663235
CTCCATG2905950.043.647721
TCCATGG2894550.043.6416362
ATGGGGG1023400.043.631235
ATGGGAT114500.043.6096085
CCATGGG2862650.043.6081243
CATGGGG2040350.043.6000674
TGGGGGG645350.043.266446
CATGGGT100000.043.2634
CATGGGA543100.043.1710554
GTACGCT800.043.12536
ATGGGAC90200.043.1186265
CATGGGC189500.042.7593654
Produced by FastQC (version 0.11.5)