##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527418_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2771152 Sequences flagged as poor quality 0 Sequence length 52 %GC 59 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.246020427605558 31.0 31.0 34.0 30.0 34.0 2 31.479407841937217 33.0 31.0 34.0 30.0 34.0 3 32.20507427957759 34.0 31.0 34.0 30.0 34.0 4 36.12586714839171 37.0 35.0 37.0 35.0 37.0 5 34.69687804927337 35.0 35.0 37.0 32.0 37.0 6 35.69469736773732 37.0 35.0 37.0 35.0 37.0 7 36.306243035387446 37.0 35.0 37.0 35.0 37.0 8 36.50113454621039 37.0 37.0 37.0 35.0 37.0 9 38.59455995196222 39.0 39.0 39.0 37.0 39.0 10 37.60937112074689 39.0 37.0 39.0 35.0 39.0 11 37.258007860990666 39.0 37.0 39.0 34.0 39.0 12 36.44390600010393 38.0 35.0 39.0 33.0 39.0 13 36.3285911418789 38.0 35.0 39.0 33.0 39.0 14 36.81942022667829 39.0 35.0 40.0 33.0 40.0 15 36.965950622701314 39.0 35.0 40.0 33.0 40.0 16 37.04051491942701 39.0 35.0 40.0 33.0 40.0 17 36.846310126618825 39.0 35.0 40.0 32.0 40.0 18 36.784544117392336 39.0 35.0 40.0 32.0 40.0 19 36.55174418436809 39.0 35.0 40.0 31.0 40.0 20 36.40898297891996 39.0 34.0 40.0 30.0 40.0 21 36.483088621627395 39.0 34.0 40.0 31.0 40.0 22 36.564057475014 39.0 35.0 40.0 31.0 40.0 23 36.626454990559886 38.0 35.0 40.0 31.0 40.0 24 36.71685782663672 38.0 35.0 40.0 32.0 40.0 25 36.75556591626876 38.0 35.0 40.0 32.0 40.0 26 36.641653002072786 38.0 35.0 40.0 32.0 40.0 27 36.57834647828773 38.0 35.0 40.0 32.0 40.0 28 36.34331389977886 38.0 35.0 40.0 31.0 40.0 29 36.28684857416699 38.0 35.0 40.0 31.0 40.0 30 36.16500791006773 38.0 35.0 40.0 31.0 40.0 31 36.08490367904756 38.0 34.0 40.0 31.0 40.0 32 35.96674632066375 38.0 35.0 40.0 31.0 40.0 33 35.86844135579715 38.0 35.0 40.0 30.0 40.0 34 35.142070157104335 37.0 33.0 40.0 27.0 40.0 35 34.96277757409193 37.0 33.0 40.0 26.0 40.0 36 35.200625949063785 37.0 34.0 40.0 29.0 40.0 37 35.32701742813097 37.0 34.0 40.0 29.0 40.0 38 35.30314396323262 37.0 34.0 40.0 29.0 40.0 39 35.191946165349286 36.0 34.0 40.0 29.0 40.0 40 35.39938083511839 37.0 34.0 40.0 30.0 40.0 41 35.443106693533956 37.0 34.0 40.0 30.0 40.0 42 35.51485338949289 37.0 34.0 40.0 30.0 40.0 43 35.45679919398142 36.0 34.0 40.0 30.0 40.0 44 35.365025808761125 36.0 34.0 40.0 30.0 40.0 45 35.255358782196 36.0 34.0 40.0 30.0 40.0 46 34.978327063979165 35.0 34.0 40.0 29.0 40.0 47 34.86963003112063 35.0 34.0 40.0 29.0 40.0 48 34.77765997678944 35.0 34.0 39.0 29.0 40.0 49 34.68751948648071 35.0 34.0 39.0 29.0 40.0 50 34.540214683279736 35.0 34.0 39.0 29.0 40.0 51 34.425890026963515 35.0 34.0 39.0 29.0 40.0 52 34.05232986137173 35.0 33.0 38.0 27.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 6.0 12 13.0 13 21.0 14 50.0 15 94.0 16 221.0 17 401.0 18 721.0 19 1277.0 20 2251.0 21 3359.0 22 5225.0 23 7986.0 24 11388.0 25 15811.0 26 21252.0 27 28101.0 28 36572.0 29 47187.0 30 60324.0 31 80260.0 32 111549.0 33 171988.0 34 366811.0 35 245514.0 36 280084.0 37 444384.0 38 698415.0 39 129887.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.45582198305975 0.3609329260899438 0.10010277314272187 0.08314231770758154 8 99.46260616523381 0.2577267504633452 0.17306881758921921 0.10659826671362668 9 98.5896118293042 0.740089320253815 0.2143873739152526 0.4559114765267297 10 70.7778570067611 18.955113252539014 3.5098038649630188 6.757225875736878 11 45.678439869050855 22.124553254386623 13.807181995069199 18.38982488149333 12 40.514522480181526 14.612659283936788 21.953000051963947 22.919818183917734 13 29.101723759649417 12.83343533663978 23.73926078396277 34.32558011974803 14 23.465475729949134 15.139119037858622 32.38353580027368 29.01186943191857 15 29.091727916765304 14.041524968677285 27.728720763061716 29.138026351495693 16 36.850378470758734 15.206166965940518 23.52184217971443 24.42161238358632 17 36.08899836602251 18.62330178929196 22.964997950310917 22.322701894374614 18 35.914883052246864 17.896275628330745 23.722336414603024 22.466504904819367 19 35.018541025537395 22.72004567053702 19.415752004942348 22.845661298983238 20 36.76611748471394 24.706042829841163 17.821577452265338 20.706262233179558 21 35.068159379204026 23.783466226320318 21.886890361842294 19.261484032633362 22 30.975132363724544 20.23598849864605 26.029535730988414 22.75934340664099 23 33.247256014827045 19.73377858738893 24.68186515932724 22.337100238456784 24 36.964410469003504 19.577670225234847 20.325987170678474 23.131932135083172 25 32.770486786722635 16.531283740480493 19.401064972257025 31.297164500539846 26 26.726033072166377 16.687031241880636 25.067444874911228 31.519490811041763 27 23.612309970726976 16.478309381802227 25.037168657655734 34.872211989815064 28 26.051620409129487 24.5302675565974 19.459091381490442 29.95902065278267 29 32.98011079868589 23.25736733315242 18.03975386409695 25.722768004064733 30 32.25965230344636 19.48139257608388 23.83997702038719 24.418978100082565 31 36.83720705323995 17.50531908751306 22.05938180222521 23.598092057021773 32 41.43309352933365 16.68977378361057 20.35146393990658 21.5256687471492 33 42.6426987765377 16.962873202191727 19.525634104516822 20.868793916753756 34 32.51510563115989 19.44671385763033 27.529417368661118 20.50876314254866 35 27.97266263272459 27.81785336928469 25.39092045474229 18.81856354324844 36 28.70986506694689 31.152711940738005 19.613864558854946 20.523558433460167 37 32.553934248283745 28.315299918589815 18.15374977626633 20.977016056860105 38 34.43762738384614 27.33155741727628 17.00588780406127 21.224927394816305 39 34.101702108004176 23.263105019140053 17.56980490424199 25.065387968613773 40 31.6800016743939 20.913684994543786 18.482205234501752 28.924108096560563 41 25.833552255524054 23.844018660831306 18.946669110896842 31.375759972747797 42 23.51022246343759 21.996772461416768 21.571714579351838 32.92129049579381 43 24.125814823582395 18.05436872463149 21.98677661853265 35.83303983325346 44 26.086768246563164 16.282686767091807 21.04803345323533 36.582511533109695 45 30.556281286627367 19.275665860263167 19.897248508923365 30.270804344186104 46 28.661437553768256 23.355196683545326 21.16935483870968 26.814010923976745 47 26.48403985057478 22.41389140689504 22.276908664699736 28.825160077830446 48 26.7407201048517 20.625248993920216 26.72643001899571 25.907600882232373 49 31.753256407443548 17.557138691778725 25.091802975802118 25.597801924975606 50 27.307451918913145 19.097941938948136 23.670336379960393 29.92426976217833 51 24.478880985236465 20.52013025629774 25.903378811411283 29.097609947054508 52 24.920069343002478 18.563795850967395 26.326488045404943 30.189646760625184 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 2.0 6 4.0 7 14.5 8 25.0 9 53.0 10 81.0 11 212.5 12 344.0 13 585.5 14 1328.0 15 1829.0 16 1797.0 17 1765.0 18 1870.5 19 1976.0 20 2105.5 21 2235.0 22 2241.5 23 2248.0 24 2724.5 25 3201.0 26 3963.0 27 4725.0 28 5787.5 29 6850.0 30 8494.0 31 10138.0 32 11105.0 33 12072.0 34 13251.0 35 14430.0 36 19663.0 37 24896.0 38 24455.0 39 26792.5 40 29571.0 41 31698.5 42 33826.0 43 37056.0 44 40286.0 45 46420.0 46 52554.0 47 62390.0 48 72226.0 49 73698.0 50 75170.0 51 82126.5 52 89083.0 53 106009.5 54 122936.0 55 140767.0 56 158598.0 57 198623.5 58 238649.0 59 256324.5 60 274000.0 61 300317.5 62 326635.0 63 364704.5 64 320560.0 65 238346.0 66 198185.0 67 158024.0 68 128876.0 69 99728.0 70 91020.5 71 82313.0 72 71015.5 73 59718.0 74 57374.5 75 55031.0 76 42653.0 77 30275.0 78 21153.5 79 12032.0 80 8231.5 81 4431.0 82 3307.0 83 2183.0 84 1465.5 85 748.0 86 494.0 87 240.0 88 157.5 89 51.5 90 28.0 91 18.0 92 8.0 93 6.0 94 4.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 2771152.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.438072650690934 #Duplication Level Percentage of deduplicated Percentage of total 1 69.29402505642574 5.8470801768491825 2 10.228074743861225 1.7261047553079616 3 3.9538222604481956 1.0008791844474276 4 1.881555952711468 0.6350682330127745 5 1.0530775593348798 0.4442972476240004 6 0.6417953734551081 0.3249309592854913 7 0.47630085706234515 0.2813342864834898 8 0.35371900489310465 0.2387765328974495 9 0.31942874524212533 0.24258266631648895 >10 7.013627649245099 16.333663858461975 >50 2.89426522203745 16.947353597033587 >100 1.720334414562968 28.52950472130499 >500 0.09700488465350068 5.5558556456044865 >1k 0.063954547846777 10.13877828507547 >5k 0.00557992699334296 3.102824354519497 >10k+ 0.0034338012266725906 8.650965495775756 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 74893 2.702594444476521 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 56013 2.0212893410393944 No Hit CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 30043 1.0841339630594062 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 23068 0.8324335871868451 No Hit CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 19163 0.6915174627736046 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT 12743 0.45984485874466646 No Hit CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 12065 0.43537849962759173 No Hit CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 10868 0.39218346738107474 No Hit CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 8783 0.3169440001847607 No Hit CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 8280 0.2987927042616212 No Hit CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG 7968 0.2875338487387195 No Hit CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 7695 0.27768235015618054 No Hit CTCCATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT 6885 0.24845262908710888 No Hit CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG 6635 0.23943111023863 No Hit CTCCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC 6395 0.23077045214409025 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC 6003 0.21662471058967533 No Hit CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTAGGTAAGAGCACCCGACTGC 5948 0.21463997644300997 No Hit CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC 5510 0.19883427542047497 No Hit CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA 5433 0.19605564761514346 No Hit CTCCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT 5106 0.18425550096133306 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT 5029 0.18147687315600156 No Hit CTCCATGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 4656 0.16801676703407104 No Hit CTCCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT 4597 0.16588768858583 No Hit CTCCATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT 4559 0.1645164177208612 No Hit CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC 4341 0.15664965328498762 No Hit CTCCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC 3910 0.14109655479021 No Hit CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT 3761 0.13571972955651657 No Hit CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 3737 0.1348536637470626 No Hit CTCCATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA 3614 0.13041507647361097 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC 3565 0.12864685877930912 No Hit CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT 3510 0.12666212463264376 No Hit CTCCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 3471 0.12525476769228105 No Hit CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT 3450 0.12449696010900882 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT 3402 0.12276482849010087 No Hit CTCCATGGGGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCGACTGCTC 3312 0.11951708170464846 No Hit CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC 3307 0.11933665132767889 No Hit CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT 3285 0.11854275766901275 No Hit CTCCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG 3193 0.11522283873277252 No Hit CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG 3133 0.11305767420913757 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT 3085 0.11132554259022961 No Hit CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG 3056 0.11027904640380608 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT 3014 0.10876343123726162 No Hit CTCCATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 2913 0.10511873762247614 No Hit CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG 2900 0.10464961864235524 No Hit CTCCATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG 2862 0.10327834777738645 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT 2860 0.10320617562659862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.330329047269872E-4 0.0 0.0 0.0 0.0 8 5.773772063026496E-4 0.0 0.0 0.0 0.0 9 0.0029951442576949946 0.0 0.0 0.0 0.0 10 0.01082582261817468 0.0 0.0 0.0 0.0 11 0.037890379163611376 0.0 0.0 0.0 0.0 12 0.046045832202636305 0.0 0.0 0.0 0.0 13 0.051061796682390574 0.0 0.0 0.0 0.0 14 0.06665098125256211 0.0 0.0 0.0 0.0 15 0.07357950772819391 0.0 0.0 0.0 0.0 16 0.08061629243000745 0.0 0.0 0.0 0.0 17 0.08685918347315484 0.0 0.0 0.0 0.0 18 0.09281338591315091 0.0 0.0 0.0 0.0 19 0.10566002875338487 0.0 0.0 0.0 0.0 20 0.11482589190343943 0.0 0.0 0.0 0.0 21 0.1216822462282834 0.0 0.0 0.0 0.0 22 0.12716732968815858 0.0 0.0 0.0 0.0 23 0.1341319422391843 0.0 0.0 0.0 0.0 24 0.1434782357662084 0.0 0.0 0.0 0.0 25 0.14975721288474975 0.0 0.0 0.0 0.0 26 0.15859830135625905 0.0 0.0 0.0 0.0 27 0.16873848854194934 0.0 0.0 0.0 0.0 28 0.222326310501914 0.0 0.0 0.0 0.0 29 0.2328995305923313 0.0 0.0 0.0 0.0 30 0.2544790036778928 0.0 0.0 0.0 0.0 31 0.26436658833582566 0.0 0.0 0.0 0.0 32 0.2722694388470932 0.0 0.0 0.0 0.0 33 0.2793783956996946 0.0 0.0 0.0 0.0 34 0.28876077530211264 0.0 0.0 0.0 0.0 35 0.32997107340196424 0.0 0.0 0.0 0.0 36 0.3472563035156498 0.0 0.0 0.0 0.0 37 0.360427721034429 0.0 0.0 0.0 0.0 38 0.3705679082201193 0.0 0.0 0.0 0.0 39 0.3786872751837503 0.0 0.0 0.0 0.0 40 0.386950986448957 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTAA 25 3.4184133E-5 46.0 33 ATATGCG 20 6.313003E-4 46.0 31 CGAATTA 25 3.4184133E-5 46.0 24 GACGAAT 20 6.313003E-4 46.0 9 CGAAATA 35 1.02027116E-7 45.999996 10 ATGGGGC 38880 0.0 43.923615 5 ATGGGAG 21355 0.0 43.738235 5 ATGGGGT 19390 0.0 43.66323 5 CTCCATG 290595 0.0 43.64772 1 TCCATGG 289455 0.0 43.641636 2 ATGGGGG 102340 0.0 43.63123 5 ATGGGAT 11450 0.0 43.609608 5 CCATGGG 286265 0.0 43.608124 3 CATGGGG 204035 0.0 43.600067 4 TGGGGGG 64535 0.0 43.26644 6 CATGGGT 10000 0.0 43.263 4 CATGGGA 54310 0.0 43.171055 4 GTACGCT 80 0.0 43.125 36 ATGGGAC 9020 0.0 43.118626 5 CATGGGC 18950 0.0 42.759365 4 >>END_MODULE