FastQCFastQC Report
Fri 17 Jun 2016
SRR1527417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117397
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC97640.461132229808581No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC84950.40120015282915766No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT83850.3960050949349602No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT72640.3430627322131844No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG66350.31335644661818257No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC42350.20000972892660185No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG38770.18310217687094107No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC31590.14919261716154317No Hit
TTAACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC29860.1410222079279417No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT29210.1379524009904614No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT25620.12099762113576246No Hit
TTAACTGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT23520.11107978333774914No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA22800.1076793818070017No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC22350.10555413085028457No Hit
TTAACTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA22020.10399561348202534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT222050.042.1472635
TAACTGG2187500.042.0308072
AACTGGG2129100.042.018133
ACTGGGG1033100.042.006774
TTAACTG2205050.041.99561
TGGGATC61550.041.6766856
ACTGGGA749350.041.6395574
CTGGGGG381650.041.5270545
ACTGGGC240750.041.2951164
CTGGGGC221900.041.1347475
TGGGGGG184300.040.944116
ACTGGGT120450.040.8401834
TGGGATT77850.040.806686
CTGGGGA270400.040.6804735
AACCCGA650.040.61538330
TGGGGAG110350.040.451296
CTGGGAC123200.040.4107135
TGGGGCT113200.040.249126
TGGGATA34850.040.2123376
CGGTACT557.8216544E-1140.012