FastQCFastQC Report
Fri 17 Jun 2016
SRR1527416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768910
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA88770.5018344630309061No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG70600.39911583969789305No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC65660.37118903731676567No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC63190.357225636126202No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT55040.31115206539620444No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC36440.2060025665522836No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT35820.20249758325748624No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC28180.15930714394740264No Hit
TTAACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC25920.14653091451798No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT22560.1275361663397233No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT21020.11883024009135569No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG20090.11357276514915966No Hit
TTAACTGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC18870.10667586253681645No Hit
TTAACTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA18110.10237943140125841No Hit
TTAACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT17970.10158798356049771No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA17880.10107919566286583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA253.4179087E-546.040
TACGCAC206.312382E-446.010
TACGATT253.4179087E-546.026
ACGTAAT301.8619758E-646.024
CACGTAA301.8619758E-646.023
GCGTAAC253.4179087E-546.033
TAACTGG1835050.043.8467062
AACTGGG1784500.043.8282433
TTAACTG1849800.043.8041951
ACTGGGG867950.043.7290154
CTGGGAT192050.043.4970055
ACTGGGA624450.043.4843444
CTGGGGG318700.043.3802955
ACTGGGC202900.043.347464
TGGGATA30600.043.2189526
GATACGG700.042.7142879
TGGGATT67450.042.6582646
TGGGGGG156000.042.5647436
CTGGGAC104400.042.5191575
CTGGGGC191850.042.4394045