##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768910
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.194961869173675	33.0	31.0	34.0	31.0	34.0
2	32.554371901340375	34.0	31.0	34.0	31.0	34.0
3	32.74655183135377	34.0	31.0	34.0	31.0	34.0
4	36.29848776930426	37.0	37.0	37.0	35.0	37.0
5	36.46219253664686	37.0	37.0	37.0	35.0	37.0
6	36.473280155576035	37.0	37.0	37.0	35.0	37.0
7	36.5884567332425	37.0	37.0	37.0	35.0	37.0
8	36.68566348768451	37.0	37.0	37.0	35.0	37.0
9	38.68672402779112	39.0	39.0	39.0	39.0	39.0
10	37.635726520851826	39.0	37.0	39.0	35.0	39.0
11	37.82998400144722	39.0	37.0	39.0	35.0	39.0
12	37.68594275570832	39.0	37.0	39.0	35.0	39.0
13	37.69450848262489	39.0	37.0	39.0	35.0	39.0
14	38.42495717701862	40.0	38.0	40.0	35.0	40.0
15	38.49020131041149	40.0	38.0	40.0	35.0	40.0
16	38.58841546489081	40.0	38.0	40.0	35.0	40.0
17	38.52963180715808	40.0	38.0	40.0	35.0	40.0
18	38.41143755193877	40.0	38.0	40.0	35.0	40.0
19	38.33067143042891	40.0	38.0	40.0	34.0	40.0
20	38.28636561498324	40.0	38.0	40.0	34.0	40.0
21	38.255605429332185	40.0	38.0	40.0	34.0	40.0
22	38.26387945118746	40.0	38.0	40.0	34.0	40.0
23	38.15685817820013	40.0	38.0	40.0	34.0	40.0
24	38.12280556953152	40.0	38.0	40.0	34.0	40.0
25	38.12713026666139	40.0	38.0	40.0	35.0	40.0
26	38.05196137734537	40.0	38.0	40.0	35.0	40.0
27	38.01298992034643	40.0	38.0	40.0	35.0	40.0
28	37.930283620986934	40.0	37.0	40.0	34.0	40.0
29	37.84633474851745	40.0	37.0	40.0	34.0	40.0
30	37.73045039035338	40.0	37.0	40.0	34.0	40.0
31	37.6909746680159	40.0	37.0	40.0	34.0	40.0
32	37.60137881520258	40.0	37.0	40.0	34.0	40.0
33	37.42211757522995	40.0	36.0	40.0	33.0	40.0
34	37.145666540411895	39.0	36.0	40.0	33.0	40.0
35	37.00764821274118	39.0	35.0	40.0	32.0	40.0
36	36.91026338253501	39.0	35.0	40.0	32.0	40.0
37	36.81833501987099	39.0	35.0	40.0	32.0	40.0
38	36.96140448072542	39.0	35.0	40.0	32.0	40.0
39	36.91458016518647	39.0	35.0	40.0	32.0	40.0
40	36.766566416606835	39.0	35.0	40.0	32.0	40.0
41	36.64819465094324	39.0	35.0	40.0	32.0	40.0
42	36.52735695993578	39.0	35.0	40.0	31.0	40.0
43	36.49318846069048	39.0	35.0	40.0	31.0	40.0
44	36.3987410326133	39.0	35.0	40.0	31.0	40.0
45	36.30560740795179	39.0	35.0	40.0	31.0	40.0
46	36.167178092723766	38.0	35.0	40.0	31.0	40.0
47	36.0371675212419	38.0	35.0	40.0	31.0	40.0
48	35.88530450955673	38.0	35.0	40.0	30.0	40.0
49	35.71723038481325	38.0	35.0	40.0	30.0	40.0
50	35.995631773238884	38.0	35.0	40.0	31.0	40.0
51	36.09228621015201	38.0	35.0	40.0	31.0	40.0
52	35.75260358073616	37.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	6.0
13	24.0
14	31.0
15	59.0
16	88.0
17	149.0
18	277.0
19	494.0
20	778.0
21	1267.0
22	1812.0
23	2795.0
24	3870.0
25	5254.0
26	7552.0
27	10551.0
28	13696.0
29	16313.0
30	18859.0
31	24278.0
32	35286.0
33	51193.0
34	104769.0
35	111062.0
36	155927.0
37	272505.0
38	690517.0
39	239494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.51195934219378	0.3637833468067906	0.10509296685529508	0.019164344144133958
8	99.22398539213414	0.6098670932947409	0.09514333685716063	0.07100417771395945
9	97.17645329609759	1.5716458157848618	0.501608335076403	0.7502925530411383
10	48.13320067160002	34.933320519415915	5.734887586140617	11.198591222843445
11	32.473726758286176	26.008276283134812	22.5773498934372	18.94064706514181
12	29.555262845481117	20.17445771689911	25.974583217913853	24.29569621970592
13	25.765584455964408	18.655499714513457	29.195210609923627	26.383705219598507
14	25.46031171738528	19.62688887506996	30.140312395769147	24.772487011775613
15	24.774635227343392	21.739319694048877	28.50275028124664	24.983294797361086
16	30.06376808316986	22.18060839726159	25.32808339598962	22.427540123578925
17	30.462770858890504	22.858370408895873	22.482715344477672	24.19614338773595
18	28.586304560435522	23.08608125908045	24.614084379646222	23.713529800837804
19	28.166045756991593	23.586728550350216	23.71268182100842	24.534543871649774
20	27.876601975227683	24.844226105341708	23.72048323543877	23.55868868399184
21	28.90944140742039	23.431152517652112	24.844508765284836	22.814897309642664
22	27.88559056141918	22.333527426494282	25.87621755770503	23.90466445438151
23	27.86461719363902	22.89228960207133	25.77168991073599	23.471403293553657
24	28.138571210519476	22.976465733135097	24.651452024127853	24.23351103221758
25	27.794404463765822	22.942433475982384	22.78764889112504	26.475513169126753
26	26.412819193740777	23.389149250103173	23.36698871056187	26.831042845594176
27	24.91720890265757	22.097902097902097	24.845695937045978	28.13919306239436
28	25.699442029272262	24.296544199535308	23.324815847047052	26.679197924145377
29	27.966487837142644	24.081213854859772	22.949726102515108	25.00257220548247
30	27.76907813286148	22.94797361086771	23.680967375389365	25.60198088088145
31	29.334166237965754	23.20739890667134	22.925756539337787	24.532678316025123
32	31.579560294192472	22.660734576660204	22.56655228360968	23.19315284553765
33	30.778049759456387	22.604089524057187	23.40073830777145	23.21712240871497
34	27.704914325771234	24.2405775307958	26.008615475066566	22.045892668366395
35	26.837713620252018	26.1684314069116	25.73098687892544	21.26286809391094
36	27.68111435855979	27.454590680136352	22.452357666585637	22.411937294718214
37	27.97847261873131	28.451871491483455	21.945717984521544	21.623937905263695
38	29.25722620144609	26.835508872695613	21.567292852660678	22.339972073197618
39	28.433781255123215	25.18901470397024	21.999479905704643	24.377724135201902
40	26.910470289613375	25.784466140165414	21.79144218756183	25.51362138265938
41	25.4226048809719	25.71470566620122	23.442685043331768	25.42000440949511
42	25.060234833880752	23.854011792572827	24.389313192870183	26.696440180676234
43	25.006020656788642	23.777128288041787	24.400110802697704	26.81674025247186
44	26.021561300461865	23.290953185860218	24.88035004607357	25.807135467604343
45	26.50434448332589	23.7170347841326	23.18484264320966	26.59377808933185
46	25.64387108445314	24.9433832133913	23.26014325205918	26.152602450096385
47	25.254648342764753	24.499154846770043	24.582596061981672	25.66360074848353
48	26.529388154287105	24.59237609601393	24.821726373868653	24.056509375830313
49	26.443798723507694	23.9799085312424	24.24922692505554	25.327065820194356
50	24.738454754622904	24.366248141510873	25.17855628607448	25.71674081779175
51	25.123154937221226	24.661514718103238	24.300671034704987	25.914659309970546
52	24.80284468966765	24.208071637335987	24.965374156966718	26.023709516029648
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	8.0
2	16.0
3	12.5
4	9.0
5	45.5
6	82.0
7	965.5
8	1849.0
9	2918.0
10	3987.0
11	4231.5
12	4476.0
13	3804.5
14	3138.0
15	3143.0
16	3614.5
17	4086.0
18	4297.0
19	4508.0
20	5562.0
21	6616.0
22	7588.0
23	8560.0
24	11058.0
25	13556.0
26	15714.5
27	17873.0
28	20157.0
29	22441.0
30	24235.0
31	26029.0
32	31415.0
33	36801.0
34	39650.0
35	42499.0
36	44638.0
37	46777.0
38	50382.0
39	57580.0
40	61173.0
41	65725.0
42	70277.0
43	73293.0
44	76309.0
45	81218.5
46	86128.0
47	88109.0
48	90090.0
49	93445.0
50	96800.0
51	99576.5
52	102353.0
53	121325.5
54	140298.0
55	146259.0
56	152220.0
57	144334.0
58	136448.0
59	128403.0
60	120358.0
61	112749.5
62	105141.0
63	91183.5
64	60638.0
65	44050.0
66	39854.5
67	35659.0
68	32965.0
69	30271.0
70	26098.0
71	21925.0
72	16941.0
73	11957.0
74	9001.5
75	6046.0
76	4264.0
77	2482.0
78	1701.5
79	921.0
80	553.0
81	185.0
82	131.5
83	78.0
84	62.0
85	46.0
86	35.5
87	25.0
88	17.5
89	8.0
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1768910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.074049345033208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.71104440081145	6.82124402497509
2	9.39909420800554	1.8937387770012724
3	3.5327399537221664	1.0676698985090232
4	1.6369924645313827	0.6596457146054668
5	1.0166544905243275	0.5120913752195836
6	0.6434525714236676	0.38893037734263197
7	0.5617746644149104	0.3961541983073694
8	0.4900181236586322	0.39491734061581163
9	0.42979264359951413	0.3896777069778399
>10	10.10702754735289	27.046916224674245
>50	2.7802737557421215	19.273734564450326
>100	1.5983462271002988	30.912357156162003
>500	0.06506542163417146	4.426660716289752
>1k	0.024894596103509078	3.859762700681261
>5k	0.002828931375398759	1.9564992241883243
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8877	0.5018344630309061	No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7060	0.39911583969789305	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6566	0.37118903731676567	No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6319	0.357225636126202	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5504	0.31115206539620444	No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	3644	0.2060025665522836	No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3582	0.20249758325748624	No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2818	0.15930714394740264	No Hit
TTAACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2592	0.14653091451798	No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	2256	0.1275361663397233	No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2102	0.11883024009135569	No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2009	0.11357276514915966	No Hit
TTAACTGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1887	0.10667586253681645	No Hit
TTAACTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1811	0.10237943140125841	No Hit
TTAACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1797	0.10158798356049771	No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1788	0.10107919566286583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0013567677270183334	0.0	0.0	0.0	0.0
8	0.0022612795450305556	0.0	0.0	0.0	0.0
9	0.0032223233516685415	0.0	0.0	0.0	0.0
10	0.016224680735594237	0.0	0.0	0.0	0.0
11	0.038328688288267916	0.0	0.0	0.0	0.0
12	0.06190252754521146	0.0	0.0	0.0	0.0
13	0.07445262902013104	0.0	0.0	0.0	0.0
14	0.08649394259741874	0.0	0.0	0.0	0.0
15	0.10107919566286583	0.0	0.0	0.0	0.0
16	0.11628630060319632	0.0	0.0	0.0	0.0
17	0.12861027412361284	0.0	0.0	0.0	0.0
18	0.142460611336925	0.0	0.0	0.0	0.0
19	0.15648054451611446	0.0	0.0	0.0	0.0
20	0.171913777410948	0.0	0.0	0.0	0.0
21	0.18587717860151168	0.0	0.0	0.0	0.0
22	0.1976923642242963	0.0	0.0	0.0	0.0
23	0.2130690651305041	0.0	0.0	0.0	0.0
24	0.22912414990022104	0.0	0.0	0.0	0.0
25	0.2415611873978891	0.0	0.0	0.0	0.0
26	0.2568813563154711	0.0	0.0	0.0	0.0
27	0.27372788892594874	0.0	0.0	0.0	0.0
28	0.29673640829663467	0.0	0.0	0.0	0.0
29	0.31143472533933325	0.0	0.0	0.0	0.0
30	0.3269244902227926	0.0	0.0	0.0	0.0
31	0.3372133121526816	0.0	0.0	0.0	0.0
32	0.3511767133432453	0.0	0.0	0.0	0.0
33	0.36140900328450853	0.0	0.0	0.0	0.0
34	0.37390257277080235	0.0	0.0	0.0	0.0
35	0.3934626408353167	0.0	0.0	0.0	0.0
36	0.40934812963915634	0.0	0.0	0.0	0.0
37	0.42280274293208814	0.0	0.0	0.0	0.0
38	0.43699227207715485	0.0	0.0	0.0	0.0
39	0.4514079291767247	0.0	0.0	0.0	0.0
40	0.46486254246965647	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	25	3.4179087E-5	46.0	40
TACGCAC	20	6.312382E-4	46.0	10
TACGATT	25	3.4179087E-5	46.0	26
ACGTAAT	30	1.8619758E-6	46.0	24
CACGTAA	30	1.8619758E-6	46.0	23
GCGTAAC	25	3.4179087E-5	46.0	33
TAACTGG	183505	0.0	43.846706	2
AACTGGG	178450	0.0	43.828243	3
TTAACTG	184980	0.0	43.804195	1
ACTGGGG	86795	0.0	43.729015	4
CTGGGAT	19205	0.0	43.497005	5
ACTGGGA	62445	0.0	43.484344	4
CTGGGGG	31870	0.0	43.380295	5
ACTGGGC	20290	0.0	43.34746	4
TGGGATA	3060	0.0	43.218952	6
GATACGG	70	0.0	42.714287	9
TGGGATT	6745	0.0	42.658264	6
TGGGGGG	15600	0.0	42.564743	6
CTGGGAC	10440	0.0	42.519157	5
CTGGGGC	19185	0.0	42.439404	5
>>END_MODULE