FastQCFastQC Report
Fri 17 Jun 2016
SRR1527415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371869
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA113380.47801965454247264No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG96930.40866506539779396No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC82720.34875450541324166No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC81160.342177413676725No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT73130.30832225557145015No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT48200.2032152703205784No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC47630.20081210218608195No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC37120.1565010546535243No Hit
TTAACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC35320.14891210264985122No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT28280.11923086814659663No Hit
TTAACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT27910.11767091690139717No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG26280.11079869925362656No Hit
TTAACTGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC25580.10784744014108705No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA25240.10641397142928215No Hit
TTAACTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA24760.10439025089496934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC351.0201802E-746.00000433
TATCACG253.4182638E-546.045
CGTATGT206.312819E-446.027
CGTATCG301.8622468E-646.018
CGTTCGA253.4182638E-546.045
TAACTGG2451350.043.977112
AACTGGG2386500.043.9529843
TTAACTG2473600.043.9013981
ACTGGGG1166850.043.8948444
TCGTAGT1050.043.80952543
CTGGGAT258800.043.671565
ACTGGGA828800.043.638394
ACTGGGC271900.043.487684
CTGGGGG427400.043.309315
CTGGGGC258650.042.6120265
TGGGATT89250.042.546786
TGGGATC71400.042.4887966
TGGGGGG201700.042.4194346
CTGGGGA313750.042.341995
TGGGATG65500.042.1374056