##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527415_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2371869 Sequences flagged as poor quality 0 Sequence length 52 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39499651962229 31.0 31.0 33.0 30.0 34.0 2 31.446727454172215 33.0 31.0 34.0 30.0 34.0 3 31.925934779703265 33.0 31.0 34.0 30.0 34.0 4 35.75854905983425 37.0 35.0 37.0 35.0 37.0 5 36.08797113162658 37.0 35.0 37.0 35.0 37.0 6 36.163602627295184 37.0 36.0 37.0 35.0 37.0 7 36.39454582019496 37.0 36.0 37.0 35.0 37.0 8 36.54267752561377 37.0 37.0 37.0 35.0 37.0 9 38.59545109784731 39.0 39.0 39.0 38.0 39.0 10 37.10669054656897 39.0 37.0 39.0 33.0 39.0 11 37.23853214490345 39.0 37.0 39.0 34.0 39.0 12 37.103977074619216 39.0 37.0 39.0 34.0 39.0 13 37.093019471142796 39.0 37.0 39.0 34.0 39.0 14 37.81109917959213 40.0 38.0 40.0 33.0 40.0 15 37.8708031514388 40.0 37.0 40.0 33.0 40.0 16 37.95030079654484 40.0 38.0 40.0 34.0 40.0 17 37.9264402882284 40.0 38.0 40.0 34.0 40.0 18 37.826910339483334 40.0 37.0 40.0 33.0 40.0 19 37.69082314411125 40.0 37.0 40.0 33.0 40.0 20 37.602727637993496 40.0 37.0 40.0 33.0 40.0 21 37.65461878375239 40.0 37.0 40.0 33.0 40.0 22 37.67099236930876 40.0 37.0 40.0 33.0 40.0 23 37.640491527989106 40.0 37.0 40.0 33.0 40.0 24 37.628535134107324 39.0 37.0 40.0 33.0 40.0 25 37.632408872496754 39.0 37.0 40.0 34.0 40.0 26 37.517130583518735 39.0 36.0 40.0 33.0 40.0 27 37.4604609276482 39.0 36.0 40.0 33.0 40.0 28 37.29525070735357 39.0 36.0 40.0 33.0 40.0 29 37.26521742979903 39.0 36.0 40.0 33.0 40.0 30 37.17886864746746 39.0 36.0 40.0 33.0 40.0 31 37.125535179219426 39.0 36.0 40.0 33.0 40.0 32 37.03412583072674 39.0 35.0 40.0 32.0 40.0 33 36.8978729432359 39.0 35.0 40.0 32.0 40.0 34 36.65994285519141 39.0 35.0 40.0 31.0 40.0 35 36.48970748384502 39.0 35.0 40.0 31.0 40.0 36 36.45878208282161 38.0 35.0 40.0 31.0 40.0 37 36.41387530255676 38.0 35.0 40.0 31.0 40.0 38 36.34853273937136 38.0 35.0 40.0 31.0 40.0 39 36.244840672060725 38.0 35.0 40.0 31.0 40.0 40 36.43776152898832 39.0 35.0 40.0 31.0 40.0 41 36.48745187866615 39.0 35.0 40.0 31.0 40.0 42 36.51504699458528 39.0 35.0 40.0 31.0 40.0 43 36.48167162689002 39.0 35.0 40.0 31.0 40.0 44 36.37268331429771 39.0 35.0 40.0 31.0 40.0 45 36.300224422175084 38.0 35.0 40.0 31.0 40.0 46 36.065281851569374 38.0 35.0 40.0 31.0 40.0 47 36.010209670095605 38.0 35.0 40.0 31.0 40.0 48 35.93874914676991 38.0 35.0 40.0 31.0 40.0 49 35.792926169194 38.0 35.0 40.0 30.0 40.0 50 35.63095263692894 37.0 35.0 40.0 30.0 40.0 51 35.50838473794295 37.0 34.0 40.0 30.0 40.0 52 35.0731187093385 37.0 34.0 40.0 29.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 6.0 13 22.0 14 22.0 15 51.0 16 98.0 17 196.0 18 359.0 19 710.0 20 1224.0 21 1965.0 22 3036.0 23 4246.0 24 6478.0 25 9261.0 26 12654.0 27 17753.0 28 23285.0 29 29346.0 30 35380.0 31 46019.0 32 63725.0 33 93883.0 34 166225.0 35 168452.0 36 250627.0 37 422829.0 38 829790.0 39 184225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.52328733163594 0.36882306737851034 0.08891722097636927 0.018972380009182634 8 99.24468847141222 0.5976721311337178 0.08621892693061885 0.0714204705234564 9 97.17842764503436 1.5758458835627094 0.48729504032473975 0.7584314310781919 10 48.00454831190087 35.039498387136895 5.669748202788603 11.286205098173635 11 32.22610523599744 26.08149944200122 22.74914845634392 18.94324686565742 12 29.47945270164583 20.104651648130652 26.087444121070767 24.328451529152748 13 25.608075319505417 18.602207794781247 29.37008747110401 26.419629414609325 14 25.25447231697872 19.709941822250723 30.202679827595873 24.83290603317468 15 24.687451119770948 21.671812397733603 28.590238331037675 25.050498151457774 16 29.89667641846999 22.111001914523946 25.444912851426448 22.54740881557961 17 30.384688193150634 22.845570307635032 22.55554585856133 24.214195640653006 18 28.535766519989085 23.03364983479273 24.65355380082121 23.77702984439697 19 28.07440039901023 23.501002795685597 23.849504336032048 24.57509246927212 20 27.721092522394784 24.78969116759821 23.79111156644823 23.69810474355877 21 28.806228337231104 23.303268435145448 25.025581092379046 22.8649221352444 22 27.785134845136895 22.211049598439036 25.967791644479522 24.036023911944547 23 27.741962140404887 22.847256741413627 25.8260468853887 23.58473423279279 24 27.908244510974257 23.050219046667415 24.717553962718853 24.323982479639476 25 27.62483931448153 22.91732806491421 22.815678268909455 26.642154351694803 26 26.336319585946775 23.371821968245296 23.339779726452008 26.952078719355914 27 24.877975975907606 22.162859753215713 24.873717730616658 28.085446540260023 28 25.843501474997144 24.20222196082499 23.31718151381885 26.637095050359022 29 27.977093169985356 23.92809215011453 23.054097844358186 25.040716835541932 30 27.75233370814324 22.853074937949778 23.778210348041988 25.616381005864998 31 29.245670819088236 23.21055673816724 22.975467869431238 24.56830457331328 32 31.502034893158097 22.656521081054645 22.682745126311783 23.15869889947548 33 30.79503969232702 22.65319037434192 23.273123431353078 23.278646501977978 34 27.592586268465922 24.22730766328157 25.99549131929293 22.184614748959575 35 26.68288172744785 26.29588733610499 25.676460209227407 21.344770727219757 36 27.615648250388197 27.37318123387084 22.554913445894357 22.456257069846604 37 27.909930944752855 28.367713393952194 21.98688038841943 21.735475272875526 38 29.105865458842793 26.73246288053851 21.70267413588187 22.45899752473682 39 28.340603970961297 25.16926525031526 22.006400859406654 24.48372991931679 40 26.769648745356513 25.781272068567024 21.91023197318233 25.538847212894133 41 25.342757125288117 25.764239087403222 23.47713975771849 25.415864029590168 42 24.754992792603637 23.922822044556426 24.54473666125743 26.77744850158251 43 24.95694323759027 23.822943004019194 24.418717897151993 26.801395861238543 44 25.908007567028363 23.382193535983646 24.854576707229615 25.85522218975837 45 26.30128392419649 23.823111647397052 23.180453895219337 26.695150533187118 46 25.55600667659133 24.98510668169279 23.330251375602952 26.128635266112926 47 25.168422033425962 24.50059425710273 24.571972566781724 25.759011142689587 48 26.46039895120683 24.602960787463388 24.82767808846104 24.108962172868736 49 26.399729496021912 24.035391499277576 24.29969783322772 25.265181171472793 50 24.62817297245337 24.329547711108834 25.262187751515786 25.78009156492201 51 25.070608874267506 24.641537960148728 24.334691334133545 25.953161831450217 52 24.753475002202904 24.23426420261827 24.904748112142787 26.10751268303604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 10.0 2 20.0 3 19.5 4 19.0 5 70.0 6 121.0 7 1201.0 8 2281.0 9 3607.0 10 4933.0 11 5333.5 12 5734.0 13 4886.0 14 4143.5 15 4249.0 16 4799.5 17 5350.0 18 5774.5 19 6199.0 20 7751.0 21 9303.0 22 10470.0 23 11637.0 24 15027.0 25 18417.0 26 21301.0 27 24185.0 28 27054.0 29 29923.0 30 32617.5 31 35312.0 32 42103.0 33 48894.0 34 52586.5 35 56279.0 36 59669.0 37 63059.0 38 67758.0 39 77992.5 40 83528.0 41 89357.5 42 95187.0 43 99249.5 44 103312.0 45 109776.0 46 116240.0 47 119378.0 48 122516.0 49 126569.0 50 130622.0 51 134112.5 52 137603.0 53 162308.0 54 187013.0 55 195572.0 56 204131.0 57 192987.0 58 181843.0 59 170837.0 60 159831.0 61 149757.5 62 139684.0 63 121126.0 64 80851.5 65 59135.0 66 53284.0 67 47433.0 68 44077.0 69 40721.0 70 35095.0 71 29469.0 72 22675.5 73 15882.0 74 11867.0 75 7852.0 76 5552.5 77 3253.0 78 2251.5 79 1250.0 80 736.0 81 222.0 82 135.0 83 48.0 84 42.0 85 36.0 86 31.0 87 26.0 88 25.0 89 27.0 90 30.0 91 15.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 2371869.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.80595781994949 #Duplication Level Percentage of deduplicated Percentage of total 1 70.42690917461101 6.906033007556493 2 9.689788372592716 1.9003531213176237 3 3.6321671731425775 1.068506342845239 4 1.6515931427909536 0.6478181077570363 5 0.9835778149941543 0.48224612832353814 6 0.6314144239294387 0.37149739247758695 7 0.45315939710105285 0.31105633535806665 8 0.368610554795455 0.289166364184993 9 0.3194291878893956 0.28190782286137206 >10 6.816659233232642 18.53102943049422 >50 3.0866785015831706 21.068954245818446 >100 1.8127495494916577 34.863305068231206 >500 0.091888542132728 5.957455269482151 >1k 0.0332179236817843 5.428370903763591 >5k 0.0017256064250277558 1.412668370417012 >10k+ 4.3140160625693895E-4 0.4796320891114595 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 11338 0.47801965454247264 No Hit TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 9693 0.40866506539779396 No Hit TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 8272 0.34875450541324166 No Hit TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 8116 0.342177413676725 No Hit TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 7313 0.30832225557145015 No Hit TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 4820 0.2032152703205784 No Hit TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC 4763 0.20081210218608195 No Hit TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 3712 0.1565010546535243 No Hit TTAACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 3532 0.14891210264985122 No Hit TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 2828 0.11923086814659663 No Hit TTAACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT 2791 0.11767091690139717 No Hit TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG 2628 0.11079869925362656 No Hit TTAACTGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 2558 0.10784744014108705 No Hit TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA 2524 0.10641397142928215 No Hit TTAACTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA 2476 0.10439025089496934 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0011383428005509579 0.0 0.0 0.0 0.0 8 0.001728594623058862 0.0 0.0 0.0 0.0 9 0.0024031681344964667 0.0 0.0 0.0 0.0 10 0.014714134718232753 0.0 0.0 0.0 0.0 11 0.034023801483134185 0.0 0.0 0.0 0.0 12 0.05173135615837131 0.0 0.0 0.0 0.0 13 0.06104890278510323 0.0 0.0 0.0 0.0 14 0.0721793657238237 0.0 0.0 0.0 0.0 15 0.08461681484095454 0.0 0.0 0.0 0.0 16 0.09865637604774968 0.0 0.0 0.0 0.0 17 0.10894362209717316 0.0 0.0 0.0 0.0 18 0.11998976334696393 0.0 0.0 0.0 0.0 19 0.13217424739730566 0.0 0.0 0.0 0.0 20 0.14583436100391717 0.0 0.0 0.0 0.0 21 0.1575972366096104 0.0 0.0 0.0 0.0 22 0.1709200634605031 0.0 0.0 0.0 0.0 23 0.18289374328852057 0.0 0.0 0.0 0.0 24 0.19946295516320672 0.0 0.0 0.0 0.0 25 0.21181608259140788 0.0 0.0 0.0 0.0 26 0.22488594437551146 0.0 0.0 0.0 0.0 27 0.23997952669392786 0.0 0.0 0.0 0.0 28 0.2628728652383416 0.0 0.0 0.0 0.0 29 0.2748887059108239 0.0 0.0 0.0 0.0 30 0.28918123218440817 0.0 0.0 0.0 0.0 31 0.29942631738936676 0.0 0.0 0.0 0.0 32 0.31097838877273576 0.0 0.0 8.432168892970058E-5 0.0 33 0.32071754384411616 0.0 0.0 8.432168892970058E-5 0.0 34 0.33231177607195 0.0 0.0 8.432168892970058E-5 0.0 35 0.35145279945899205 0.0 0.0 8.432168892970058E-5 0.0 36 0.36511291306560356 0.0 0.0 8.432168892970058E-5 0.0 37 0.3800378520061605 0.0 0.0 8.432168892970058E-5 0.0 38 0.39234881858989684 0.0 0.0 8.432168892970058E-5 0.0 39 0.40596677135204345 0.0 0.0 8.432168892970058E-5 0.0 40 0.41844638131363915 0.0 0.0 8.432168892970058E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAAC 35 1.0201802E-7 46.000004 33 TATCACG 25 3.4182638E-5 46.0 45 CGTATGT 20 6.312819E-4 46.0 27 CGTATCG 30 1.8622468E-6 46.0 18 CGTTCGA 25 3.4182638E-5 46.0 45 TAACTGG 245135 0.0 43.97711 2 AACTGGG 238650 0.0 43.952984 3 TTAACTG 247360 0.0 43.901398 1 ACTGGGG 116685 0.0 43.894844 4 TCGTAGT 105 0.0 43.809525 43 CTGGGAT 25880 0.0 43.67156 5 ACTGGGA 82880 0.0 43.63839 4 ACTGGGC 27190 0.0 43.48768 4 CTGGGGG 42740 0.0 43.30931 5 CTGGGGC 25865 0.0 42.612026 5 TGGGATT 8925 0.0 42.54678 6 TGGGATC 7140 0.0 42.488796 6 TGGGGGG 20170 0.0 42.419434 6 CTGGGGA 31375 0.0 42.34199 5 TGGGATG 6550 0.0 42.137405 6 >>END_MODULE