FastQCFastQC Report
Fri 17 Jun 2016
SRR1527414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254596
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGA25310.9941240239438169No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAA17060.6700812267278354No Hit
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAA16740.6575122939873368No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13990.5494980282486763No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAA9410.3696051784002891No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8490.3334694967713554No Hit
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAA8380.32914892614180896No Hit
TAGAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA7500.2945843611054376Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6430.2525569922543952Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6070.23841694292133417Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA5990.2352747097362095Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAA5980.23488193058806894No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAA5810.228204685069679No Hit
TAGAGAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5810.228204685069679Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAA5780.22702634762525728No Hit
TAGAGAGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA5740.22545523103269494Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.19403289918144825No Hit
TAGAGAGGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAA4450.17478672092255967No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAA4310.16928781284859148No Hit
TAGAGAGGGGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCT4130.16221778818206098No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAA4080.16025389244135807No Hit
TAGAGAGGGGCGGTGGATCAGTCAGCATATTTAGCGACTGACACACCGCC3950.1551477635155305No Hit
TAGAGAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA3870.1520055303304058Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTTTCGAGGAAGCTGCGGTCCGTCTTGCGGGTCAGGCGCGG3860.15161275118226522No Hit
TAGAGAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA3840.15082719288598406Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3820.1500416345897029No Hit
TAGAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT3720.14611384310829706No Hit
TAGAGAGGGATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAG3720.14611384310829706No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAA3530.138651039293626No Hit
TAGAGAGGGCGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA3510.1378654809973448No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAA3420.13433046866407958No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGAGA3220.1264748857012679No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAA3160.12411821081242438No Hit
TAGAGAGGGGGCCACCTCTCCTCACCCCTCCCTTCACTTAACAAACTCCA3120.12254709421986205No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAA3060.12019041933101855No Hit
TAGAGAGGGCGTACCCAGGCTGGCACTGAGCCATCAGTGTCCTACCTCAG3010.11822652359031564No Hit
TAGAGAGGATATGTGAATGTGTATGCCTTTGTGTGTGTGCTTGTGTATGT3010.11822652359031564No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGGAGGGGAAATGAGGAAAGGAGATA2910.11429873210890981No Hit
TAGAGAGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAA2800.10997816147936337No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG2740.1076214865905199Illumina Single End Adapter 2 (100% over 23bp)
TAGAGAGGGGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAAAA2740.1076214865905199No Hit
TAGAGAGGGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAA2740.1076214865905199No Hit
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAA2740.1076214865905199No Hit
TAGAGAGGGAACACAATAATATTGTAAATACAATCATTATTGTAAACAAT2690.10565759084981696No Hit
TAGAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA2670.10487203255353579Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAA2630.10330091596097346No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA2550.10015868277584879No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGC408.269126E-944.033
GGAGATA408.269126E-944.044
TGTGAAT408.269126E-944.012
TAGCGAC408.269126E-944.032
TTTTGCG351.4401485E-744.018
CTTCACT302.519926E-644.032
GGAGACT207.844923E-444.08
CATGATT551.8189894E-1244.032
ACAAACT302.519926E-644.041
AATCCGC254.433048E-544.011
TCCCAGC207.844923E-444.011
CCGGACG207.844923E-444.021
ACGCCGC207.844923E-444.025
GTGTCCT207.844923E-444.037
TATATTA302.519926E-644.024
CACACCG351.4401485E-744.042
TAAATAT302.519926E-644.041
TAAATAC207.844923E-444.025
CAGCAAA207.844923E-444.043
CTCCCTT302.519926E-644.028
Produced by FastQC (version 0.11.5)