##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254596
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.380544077676003	31.0	30.0	33.0	28.0	33.0
2	28.137244889943283	30.0	27.0	31.0	16.0	33.0
3	30.682288802652046	31.0	30.0	33.0	28.0	33.0
4	32.96633489921287	35.0	32.0	35.0	28.0	35.0
5	34.74210121133089	35.0	35.0	35.0	33.0	37.0
6	33.08152523998806	35.0	32.0	35.0	28.0	37.0
7	34.983361875284764	35.0	35.0	35.0	33.0	37.0
8	35.91380461594055	37.0	35.0	37.0	35.0	37.0
9	38.33577118257946	39.0	39.0	39.0	37.0	39.0
10	35.5194975569137	38.0	35.0	39.0	30.0	39.0
11	35.94526622570661	37.0	35.0	39.0	31.0	39.0
12	36.39154189382394	37.0	35.0	39.0	32.0	39.0
13	36.549254505176826	38.0	35.0	39.0	32.0	39.0
14	37.54477289509654	39.0	37.0	40.0	33.0	40.0
15	37.67659350500401	39.0	37.0	40.0	33.0	40.0
16	37.68028955678801	39.0	37.0	40.0	33.0	40.0
17	37.72796116199783	40.0	37.0	40.0	33.0	40.0
18	37.55384216562711	39.0	37.0	40.0	33.0	40.0
19	37.41258307278983	39.0	37.0	40.0	33.0	40.0
20	37.40857279768731	39.0	37.0	40.0	33.0	40.0
21	37.34687504909739	39.0	37.0	40.0	32.0	40.0
22	37.30590425615485	39.0	36.0	40.0	33.0	40.0
23	36.93875787522192	38.0	36.0	40.0	32.0	40.0
24	36.48932033496206	38.0	36.0	39.0	32.0	40.0
25	35.77460368583952	37.0	35.0	39.0	31.0	40.0
26	34.839636129397164	36.0	34.0	38.0	29.0	40.0
27	34.741484548068314	36.0	33.0	38.0	29.0	40.0
28	34.613363917736336	36.0	33.0	38.0	28.0	40.0
29	34.09499756476928	35.0	33.0	38.0	26.0	40.0
30	33.60351301670097	35.0	33.0	38.0	25.0	39.0
31	33.62848984273123	35.0	33.0	38.0	24.0	39.0
32	33.174629609263306	35.0	33.0	38.0	22.0	39.0
33	32.83777435623497	35.0	32.0	38.0	21.0	40.0
34	32.46703797388804	35.0	31.0	38.0	20.0	40.0
35	32.12198149224654	35.0	31.0	38.0	18.0	40.0
36	31.424024729375166	35.0	30.0	38.0	15.0	40.0
37	30.518986944021115	35.0	27.0	38.0	10.0	40.0
38	29.609286870178636	34.0	24.0	38.0	9.0	39.0
39	28.47617401687379	33.0	21.0	37.0	8.0	39.0
40	27.75172430046034	33.0	18.0	37.0	8.0	39.0
41	27.18193530141872	32.0	17.0	36.0	8.0	39.0
42	26.798559286084622	32.0	17.0	35.0	8.0	38.0
43	26.622857389746894	32.0	17.0	35.0	8.0	38.0
44	26.052958412543795	31.0	15.0	35.0	8.0	37.0
45	25.72712454241229	31.0	15.0	35.0	8.0	37.0
46	25.396864051281245	31.0	14.0	35.0	8.0	37.0
47	25.113033983251896	30.0	13.0	35.0	8.0	36.0
48	24.491017140882025	29.0	12.0	34.0	8.0	35.0
49	23.460643529356314	27.0	10.0	33.0	8.0	35.0
50	23.354015774010588	27.0	10.0	33.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	5.0
14	6.0
15	20.0
16	29.0
17	88.0
18	248.0
19	422.0
20	784.0
21	1305.0
22	2117.0
23	3058.0
24	4316.0
25	6028.0
26	8497.0
27	11600.0
28	15352.0
29	16658.0
30	14696.0
31	12630.0
32	13351.0
33	17473.0
34	25874.0
35	40084.0
36	45153.0
37	14709.0
38	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.16848654338638	0.4222375842511273	0.33307671762321484	0.0761991547392732
8	97.31181951012584	1.7451177551886126	0.5628525192854562	0.3802102154000848
9	94.4127166177002	2.9116718251661453	1.4638878851199548	1.2117236720137001
10	45.022702634762524	36.51274961114864	6.568838473503119	11.895709280585711
11	25.312652201919907	49.35073606812361	14.277522034910211	11.05908969504627
12	40.6565696240318	38.578767930368116	12.420069443353391	8.344593002246697
13	23.7195399770617	42.591006928624175	15.261041021854233	18.428412072459896
14	22.292573331866958	49.83071218715141	12.272777262800673	15.60393721818096
15	28.19525837012365	46.65862778676806	12.559113261795158	12.58700058131314
16	22.70538421656271	47.86092475922638	19.214755927037345	10.21893509717356
17	29.09315150277302	45.487753146160976	13.184810444783107	12.234284906282895
18	26.58054329211771	45.534101085641566	15.598045530958851	12.287310091281874
19	22.160992317239863	44.49362912221716	20.00581313139248	13.339565429150497
20	20.233232258165877	46.06396015648321	17.430360257034675	16.272447328316233
21	19.517588650253735	43.716319188046945	24.744693553708622	12.0213986079907
22	21.52861788873352	46.52154786406699	15.491209602664613	16.458624644534872
23	20.89388678533834	51.32091627519678	14.57917642068218	13.2060205187827
24	22.47678675234489	49.86724064792848	18.0548791025782	9.601093497148423
25	24.587581894452388	47.33931405049569	12.939716256343385	15.133387798708542
26	28.334302188565413	44.46024289462521	14.557573567534446	12.64788134927493
27	26.174409652940344	46.113450329148925	14.949567157378748	12.762572860531979
28	27.61237411428302	48.94931577872394	13.85881946299235	9.579490644000693
29	31.9054502034596	45.479112004901886	14.735502521642129	7.879935269996387
30	27.310719728511053	51.08289211142358	12.665163631793117	8.941224528272244
31	28.9816022247011	50.186570095366775	11.334820657040959	9.497007022891168
32	25.84329683105783	50.13590158525664	15.120818865968044	8.899982717717482
33	26.79853571933573	53.632814341152255	10.363477823689296	9.205172115822716
34	25.774953259281368	51.43521500730569	11.419660953039324	11.370170780373613
35	23.88097220694748	56.3217803893227	10.503307200427344	9.293940203302487
36	27.422661785731115	52.63908309635658	10.92475922638219	9.013495891530109
37	23.798095806689815	52.00945812188722	14.5261512356832	9.666294835739759
38	25.797734449873527	51.97253688196201	12.258637213467612	9.971091454696852
39	24.368803908938084	50.21602853147732	12.114880045248158	13.30028751433644
40	23.67358481672925	51.923046709296294	13.639255919181762	10.76411255479269
41	22.850319722226587	51.71330264418923	14.890257506009522	10.546120127574667
42	21.42728086851325	55.872833822998004	11.231126961931844	11.468758346556898
43	24.634322613081117	53.878694087888256	11.281795472041981	10.20518782698864
44	22.028232965168346	56.25382959669437	10.981712202862575	10.73622523527471
45	23.53336266084306	55.042105924680676	10.651384939276344	10.773146475199924
46	20.481075900642587	57.085343053307994	11.153749469748151	11.279831576301277
47	22.352668541532466	53.35747615830571	11.97505066851011	12.314804631651715
48	20.805118697858568	54.42897767443322	12.14276736476614	12.623136262942072
49	20.057659978947036	54.122609938883556	11.78690945655077	14.032820625618628
50	18.570598124086786	53.66187999811466	14.51986676931295	13.247655108485601
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	12.0
4	24.0
5	34.0
6	44.0
7	69.5
8	95.0
9	934.5
10	1774.0
11	1694.0
12	1614.0
13	1451.5
14	1289.0
15	1247.5
16	1206.0
17	1547.0
18	1888.0
19	2487.5
20	3087.0
21	4121.0
22	5155.0
23	6926.0
24	8697.0
25	9604.5
26	10512.0
27	11179.5
28	11847.0
29	12393.0
30	12939.0
31	14246.0
32	15553.0
33	15536.0
34	15519.0
35	14299.5
36	13080.0
37	12897.0
38	12714.0
39	14248.0
40	15782.0
41	16046.0
42	16310.0
43	15442.0
44	14574.0
45	14489.0
46	14404.0
47	13012.5
48	11621.0
49	11684.0
50	11747.0
51	13728.0
52	15709.0
53	14415.0
54	13121.0
55	11567.5
56	10014.0
57	7877.5
58	5741.0
59	4516.5
60	3292.0
61	2510.5
62	1729.0
63	1651.5
64	1574.0
65	1420.5
66	1267.0
67	749.5
68	232.0
69	191.0
70	150.0
71	132.0
72	114.0
73	136.0
74	158.0
75	85.0
76	12.0
77	8.0
78	4.0
79	3.5
80	3.0
81	2.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	254596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.926410723579785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.20715578160659	34.047245669933645
2	8.143500992173768	6.828555346515137
3	2.7663395525184	3.47948064839311
4	1.4798260550723439	2.4817517993767155
5	1.0190294982231518	2.1362124640973637
6	0.7614734171656183	1.9155508345904113
7	0.6447005124229436	1.8920984934282676
8	0.5349208998228757	1.7941850680480609
9	0.4470979520609492	1.6870691134600868
>10	2.627698337256943	20.369099156056812
>50	0.2295368845137643	6.688442098952812
>100	0.12275233389214353	10.216140274509344
>500	0.01197583745289205	3.405519564803062
>1k	0.003991945817630683	3.0586494678351617
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGA	2531	0.9941240239438169	No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAA	1706	0.6700812267278354	No Hit
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAA	1674	0.6575122939873368	No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1399	0.5494980282486763	No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAA	941	0.3696051784002891	No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	849	0.3334694967713554	No Hit
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAA	838	0.32914892614180896	No Hit
TAGAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	750	0.2945843611054376	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	643	0.2525569922543952	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	607	0.23841694292133417	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	599	0.2352747097362095	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAA	598	0.23488193058806894	No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAA	581	0.228204685069679	No Hit
TAGAGAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	581	0.228204685069679	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAA	578	0.22702634762525728	No Hit
TAGAGAGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	574	0.22545523103269494	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	494	0.19403289918144825	No Hit
TAGAGAGGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAA	445	0.17478672092255967	No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAA	431	0.16928781284859148	No Hit
TAGAGAGGGGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCT	413	0.16221778818206098	No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAA	408	0.16025389244135807	No Hit
TAGAGAGGGGCGGTGGATCAGTCAGCATATTTAGCGACTGACACACCGCC	395	0.1551477635155305	No Hit
TAGAGAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	387	0.1520055303304058	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTTTCGAGGAAGCTGCGGTCCGTCTTGCGGGTCAGGCGCGG	386	0.15161275118226522	No Hit
TAGAGAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	384	0.15082719288598406	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	382	0.1500416345897029	No Hit
TAGAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	372	0.14611384310829706	No Hit
TAGAGAGGGATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAG	372	0.14611384310829706	No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAA	353	0.138651039293626	No Hit
TAGAGAGGGCGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA	351	0.1378654809973448	No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAA	342	0.13433046866407958	No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGAGA	322	0.1264748857012679	No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAA	316	0.12411821081242438	No Hit
TAGAGAGGGGGCCACCTCTCCTCACCCCTCCCTTCACTTAACAAACTCCA	312	0.12254709421986205	No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAA	306	0.12019041933101855	No Hit
TAGAGAGGGCGTACCCAGGCTGGCACTGAGCCATCAGTGTCCTACCTCAG	301	0.11822652359031564	No Hit
TAGAGAGGATATGTGAATGTGTATGCCTTTGTGTGTGTGCTTGTGTATGT	301	0.11822652359031564	No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGGAGGGGAAATGAGGAAAGGAGATA	291	0.11429873210890981	No Hit
TAGAGAGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAA	280	0.10997816147936337	No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	274	0.1076214865905199	Illumina Single End Adapter 2 (100% over 23bp)
TAGAGAGGGGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAAAA	274	0.1076214865905199	No Hit
TAGAGAGGGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAA	274	0.1076214865905199	No Hit
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAA	274	0.1076214865905199	No Hit
TAGAGAGGGAACACAATAATATTGTAAATACAATCATTATTGTAAACAAT	269	0.10565759084981696	No Hit
TAGAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	267	0.10487203255353579	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAA	263	0.10330091596097346	No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	255	0.10015868277584879	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.1095853823312228	0.0	0.0	0.0	0.0
5	0.1095853823312228	0.0	0.0	0.0	0.0
6	0.8189445238731167	0.0	0.0	0.0	0.0
7	0.8260145485396472	0.0	0.0	0.0	0.0
8	0.8637213467611431	0.0	0.0	0.0	0.0
9	0.8986786909456551	0.0	0.0	0.0	0.0
10	1.2635705195682572	0.0	0.0	0.0	0.0
11	1.984320256406228	0.0	0.0	0.0	0.0
12	2.338999827177175	0.0	0.0	0.0	0.0
13	2.5542428003582147	0.0	0.0	0.0	0.0
14	2.785196939464878	0.0	0.0	0.0	0.0
15	3.008295495608729	0.0	0.0	0.0	0.0
16	3.1905450203459598	0.0	0.0	0.0	0.0
17	3.3720089867869096	0.0	0.0	0.0	0.0
18	3.544046253672485	0.0	0.0	0.0	0.0
19	3.7031218086694215	0.0	0.0	0.0	0.0
20	3.9077597448506656	0.0	0.0	0.0	0.0
21	4.113576018476331	0.0	0.0	0.0	0.0
22	4.365347452434445	0.0	0.0	0.0	0.0
23	4.63086615657748	0.0	0.0	0.0	0.0
24	4.935662775534572	0.0	0.0	0.0	0.0
25	5.223177111973479	0.0	0.0	0.0	0.0
26	5.562145516818803	0.0	0.0	0.0	0.0
27	5.909755062923219	0.0	0.0	0.0	0.0
28	6.337491555248315	0.0	0.0	0.0	0.0
29	6.578265173058493	0.0	0.0	0.0	0.0
30	6.8426055397571055	0.0	0.0	0.0	0.0
31	7.057062954641864	0.0	0.0	0.0	0.0
32	7.258558657637983	0.0	0.0	0.0	0.0
33	7.474979968263445	0.0	0.0	0.0	0.0
34	7.683152916777954	0.0	0.0	0.0	0.0
35	7.97655894043897	0.0	0.0	0.0	0.0
36	8.163521814953887	0.0	0.0	0.0	0.0
37	8.359518609876039	0.0	0.0	0.0	0.0
38	8.572797687316376	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATGGC	40	8.269126E-9	44.0	33
GGAGATA	40	8.269126E-9	44.0	44
TGTGAAT	40	8.269126E-9	44.0	12
TAGCGAC	40	8.269126E-9	44.0	32
TTTTGCG	35	1.4401485E-7	44.0	18
CTTCACT	30	2.519926E-6	44.0	32
GGAGACT	20	7.844923E-4	44.0	8
CATGATT	55	1.8189894E-12	44.0	32
ACAAACT	30	2.519926E-6	44.0	41
AATCCGC	25	4.433048E-5	44.0	11
TCCCAGC	20	7.844923E-4	44.0	11
CCGGACG	20	7.844923E-4	44.0	21
ACGCCGC	20	7.844923E-4	44.0	25
GTGTCCT	20	7.844923E-4	44.0	37
TATATTA	30	2.519926E-6	44.0	24
CACACCG	35	1.4401485E-7	44.0	42
TAAATAT	30	2.519926E-6	44.0	41
TAAATAC	20	7.844923E-4	44.0	25
CAGCAAA	20	7.844923E-4	44.0	43
CTCCCTT	30	2.519926E-6	44.0	28
>>END_MODULE