FastQCFastQC Report
Fri 17 Jun 2016
SRR1527413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204040
Sequences flagged as poor quality0
Sequence length52
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAA13880.6802587727896491No Hit
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAA13830.6778082728876691No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG13450.659184473632621No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11210.5494020780239169No Hit
TAGAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8000.3920799843168006Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7720.3783571848657126Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAA7620.3734561850617526No Hit
TAGAGAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7030.34454028621838856Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAA6910.3386590864536365No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6870.33669868653205254No Hit
TAGAGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6720.3293471868261125Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA6030.29553028817878846Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5890.28866888845324445Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAA4720.23132719074691238No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAA4640.22740639090374437No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAA4320.21172319153107233No Hit
TAGAGAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4030.1975102920995883Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGCGGTGGATCAGTCAGCATATTTAGCGACTGACACACCGCCTT3780.1852577925896883No Hit
TAGAGAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3730.1828072926877083Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGT3670.1798666928053323No Hit
TAGAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3630.1779062928837483No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA3500.17153499313860027No Hit
TAGAGAGGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAA3450.16908449323662025No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAA3320.16271319349147226No Hit
TAGAGAGGGATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAGTG3080.15095079396196823No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAA3080.15095079396196823No Hit
TAGAGAGGGGTTTCGAGGAAGCTGCGGTCCGTCTTGCGGGTCAGGCGCGGCA3040.14899039404038422No Hit
TAGAGAGGATATGTGAATGTGTATGCCTTTGTGTGTGTGCTTGTGTATGTAC2870.14065869437365222No Hit
TAGAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.13869829445206822No Hit
TAGAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT2740.13428739462850423No Hit
TAGAGAGGGCGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAA2610.1279160948833562No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGGAGGGGAAATGAGGAAAGGAGATAAA2590.1269358949225642No Hit
TAGAGAGGGAAGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA2560.12546559498137622No Hit
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAA2530.1239952950401882No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAA2460.12056459517741619No Hit
TAGAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2420.11860419525583218Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGGCCACCTCTCCTCACCCCTCCCTTCACTTAACAAACTCCAGG2410.11811409527543619No Hit
TAGAGAGGGAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA2390.11713389531464419Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAAAAAA2260.11076259556949619No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAAAA2250.11027249558910018No Hit
TAGAGAGGGGACAGAGACCGGAAGCTGGGAGATGATGGCTTCTGATGTTTGT2230.10929229562830817No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAA2200.10782199568712017No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC2080.10194079592236815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAACG351.0140502E-746.0000049
GGAGATA301.8538303E-646.044
GAGGTGG405.5715645E-946.038
TGTGAAT301.8538303E-646.012
TAGCGAC551.8189894E-1246.032
GGGTTTC405.5715645E-946.08
TCCTTCG253.407213E-546.016
CTGAGTT253.407213E-546.024
TCCCAGC206.299194E-446.011
ACGCCGG253.407213E-546.027
CCGGACG206.299194E-446.021
ACGCCGC206.299194E-446.025
TCCCAAA206.299194E-446.026
CAGCATA551.8189894E-1246.023
TATACTG206.299194E-446.036
CCTACCT301.8538303E-646.041
CAGTCAG551.8189894E-1246.019
CTGAGCC301.8538303E-646.026
ATCCATC301.8538303E-646.023
CGGAGAT206.299194E-446.018
Produced by FastQC (version 0.11.5)