##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527413_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	204040
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.665541070378357	31.0	30.0	33.0	28.0	33.0
2	29.217540678298374	31.0	28.0	31.0	25.0	33.0
3	31.059870613605174	31.0	31.0	33.0	28.0	34.0
4	33.75513624779455	35.0	33.0	35.0	30.0	37.0
5	35.172235836110566	35.0	35.0	37.0	33.0	37.0
6	33.8318123897275	35.0	33.0	35.0	32.0	37.0
7	35.2232650460694	35.0	35.0	35.0	35.0	37.0
8	36.20688100372476	37.0	35.0	37.0	35.0	37.0
9	38.402391687904334	39.0	39.0	39.0	37.0	39.0
10	36.19275142128994	39.0	35.0	39.0	31.0	39.0
11	36.42771515389139	38.0	35.0	39.0	31.0	39.0
12	36.88871789845128	39.0	37.0	39.0	34.0	39.0
13	36.94379043324838	39.0	37.0	39.0	33.0	39.0
14	37.83322877867085	40.0	38.0	40.0	34.0	40.0
15	38.058968829641245	40.0	38.0	40.0	34.0	40.0
16	38.02719074691237	40.0	38.0	40.0	34.0	40.0
17	38.14849539306019	40.0	38.0	40.0	35.0	40.0
18	38.05272985689081	40.0	38.0	40.0	34.0	40.0
19	37.90833169966673	40.0	38.0	40.0	34.0	40.0
20	38.08850225445991	40.0	38.0	40.0	34.0	40.0
21	37.964546167418156	40.0	38.0	40.0	34.0	40.0
22	37.96934914722603	40.0	38.0	40.0	34.0	40.0
23	37.70523426779063	39.0	37.0	40.0	34.0	40.0
24	37.324402078023915	39.0	37.0	40.0	33.0	40.0
25	36.61907469123701	38.0	35.0	40.0	33.0	40.0
26	36.235365614585376	37.0	35.0	40.0	32.0	40.0
27	36.05039207998432	37.0	35.0	40.0	32.0	40.0
28	35.93830621446775	37.0	35.0	40.0	31.0	40.0
29	35.744853950205844	37.0	35.0	40.0	31.0	40.0
30	35.31318859047246	37.0	35.0	40.0	30.0	40.0
31	35.162321113507154	37.0	35.0	40.0	30.0	40.0
32	34.78012644579494	37.0	35.0	40.0	25.0	40.0
33	34.368859047245635	36.0	35.0	40.0	24.0	40.0
34	33.955748872770044	37.0	34.0	40.0	20.0	40.0
35	33.563134679474615	37.0	33.0	40.0	17.0	40.0
36	32.94292785728289	37.0	33.0	40.0	14.0	40.0
37	32.0750049009998	37.0	31.0	40.0	10.0	40.0
38	31.4341599686336	37.0	29.0	40.0	9.0	40.0
39	30.624769653009213	36.0	24.0	40.0	8.0	40.0
40	29.876083120956675	35.0	20.0	40.0	8.0	40.0
41	29.4122524995099	35.0	18.0	39.0	8.0	40.0
42	28.791271319349146	35.0	15.0	39.0	8.0	40.0
43	28.562183885512646	35.0	15.0	39.0	8.0	40.0
44	28.180699862772006	35.0	13.0	38.0	8.0	40.0
45	27.84587825916487	35.0	12.0	38.0	8.0	40.0
46	27.55237698490492	35.0	10.0	37.0	8.0	40.0
47	27.330974318761026	35.0	10.0	37.0	8.0	40.0
48	27.008336600666535	35.0	10.0	35.0	8.0	40.0
49	26.66378651244854	35.0	10.0	35.0	8.0	39.0
50	26.21578121936875	34.0	10.0	35.0	8.0	39.0
51	25.969618702215254	34.0	10.0	35.0	8.0	39.0
52	25.411590864536365	33.0	9.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	4.0
15	5.0
16	40.0
17	73.0
18	157.0
19	320.0
20	564.0
21	970.0
22	1687.0
23	2724.0
24	3692.0
25	5274.0
26	7982.0
27	11115.0
28	12585.0
29	10131.0
30	6617.0
31	5128.0
32	5752.0
33	7299.0
34	10876.0
35	16673.0
36	33357.0
37	42328.0
38	17236.0
39	1446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.13105273475789	0.44059988237600467	0.35189178592432857	0.07645559694177612
8	97.21329151146834	1.784944128602235	0.5891001764359929	0.4126641834934327
9	94.22760243089591	3.0146049794158007	1.5212703391491864	1.2365222505391098
10	45.327386786904526	36.15614585375417	6.530582238776711	11.985885120564594
11	25.687610272495586	48.847774946089004	14.240345030386198	11.22426975102921
12	39.792197608312094	39.389335424426584	12.63722799451088	8.181238972750442
13	23.980101940795922	43.425308762987655	15.408743383650265	17.185845912566165
14	22.301999607920013	49.88090570476377	12.520094099196236	15.297000588119976
15	27.09223681631053	47.727406390903745	12.526465398941383	12.653891393844344
16	22.642128994314838	49.090864536365416	18.13614977455401	10.13085669476573
17	28.16800627327975	46.504116839835326	13.125857674965694	12.20201921191923
18	25.40874338365027	46.72319153107234	15.640070574397177	12.227994510880219
19	22.37257400509704	45.59939227602431	18.80758674769653	13.22044697118212
20	20.32591648696334	46.037541658498334	17.306410507743582	16.330131346794747
21	19.74122721035091	44.743677710252896	23.58214075671437	11.932954322681827
22	21.530092138796313	47.42109390315624	15.610174475593022	15.438639482454422
23	20.833660066653596	51.34679474612821	14.764261909429525	13.055283277788668
24	22.440207802391686	49.83189570672417	18.159674573613017	9.568221917271122
25	24.560870417565184	48.40962556361498	12.91168398353264	14.117820035287199
26	27.03587531856499	45.82336796706528	14.826504606939817	12.314252107429915
27	25.944912762203487	47.25102920995884	15.1837874926485	11.620270535189178
28	27.549500098019998	48.89041364438346	13.97814154087434	9.58194471672221
29	31.177220152911193	46.31248774750049	14.689276612428937	7.82101548715938
30	27.32699470692021	50.96500686139973	12.68868849245246	9.019309939227602
31	28.107233875710648	50.93511076259557	11.351205645951774	9.606449715742011
32	26.10664575573417	50.934620662615174	14.230052930797882	8.728680650852775
33	26.044893158204275	54.34032542638698	10.412174083513037	9.202607331895706
34	26.169868653205253	51.953538521858455	11.34924524603019	10.527347578906097
35	24.67408351303666	55.414134483434616	10.571946677122133	9.339835326406586
36	27.525975298960986	52.58037639678494	11.020878259164869	8.872770045089199
37	25.153891393844347	52.03146441874142	13.28906096843756	9.52558321897667
38	26.32964124681435	52.15987061360517	11.84914722603411	9.661340913546363
39	25.64350127425995	50.61605567535777	11.825622426975103	11.914820623407175
40	25.110272495589097	51.56537933738483	13.022936679082534	10.30141148794354
41	24.646637914134484	51.32425014702999	14.077141736914331	9.951970201921192
42	24.12762203489512	53.429719662811216	11.61634973534601	10.826308566947656
43	26.787884728484613	52.28288570868457	11.38600274455989	9.543226818270927
44	24.97892570084297	53.47088806116448	10.940991962360322	10.609194275632229
45	25.558223877671043	53.35130366594786	10.628308174867673	10.462164281513429
46	22.282395608704174	54.97010390119584	11.168398353264065	11.579102136835914
47	23.493432660262695	52.07655361693786	11.82464222701431	12.605371495785139
48	22.465202901391883	52.74799059008038	12.177514212899432	12.609292295628308
49	21.652617133895312	52.01529111938835	12.46814350127426	13.86394824544207
50	18.569398157224075	53.194471672221134	14.360419525583218	13.875710644971576
51	17.836698686532053	52.44902960203882	14.67408351303666	15.040188198392473
52	19.64075671436973	49.27171142913154	16.268868849245248	14.81866300725348
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	12.0
4	24.0
5	33.0
6	42.0
7	63.0
8	84.0
9	761.5
10	1439.0
11	1309.0
12	1179.0
13	1074.5
14	868.0
15	766.0
16	855.5
17	945.0
18	1131.5
19	1318.0
20	1651.5
21	1985.0
22	2734.0
23	3483.0
24	4107.5
25	4732.0
26	5708.0
27	6684.0
28	8133.5
29	9583.0
30	11917.5
31	14252.0
32	14708.0
33	15164.0
34	14510.5
35	13857.0
36	12934.0
37	12011.0
38	12995.5
39	14269.0
40	14558.0
41	13519.0
42	12480.0
43	12440.5
44	12401.0
45	10868.0
46	9335.0
47	8148.5
48	6962.0
49	6527.0
50	6092.0
51	7876.5
52	9661.0
53	9571.5
54	9482.0
55	8455.5
56	7429.0
57	6087.5
58	4746.0
59	3796.5
60	2847.0
61	2564.5
62	2282.0
63	1804.0
64	1240.0
65	1154.0
66	759.0
67	364.0
68	242.5
69	121.0
70	101.0
71	81.0
72	132.5
73	184.0
74	105.5
75	27.0
76	18.0
77	9.0
78	4.5
79	0.0
80	0.5
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	204040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.64399137424034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17905768157024	37.50980199960792
2	7.661169093329898	6.9937267202509315
3	2.686509470429068	3.678690452852382
4	1.5268650947042908	2.7876886884924525
5	1.060859854829704	2.421093903156244
6	0.8020873598763045	2.1966281121348756
7	0.6184770003865481	1.9760831209566752
8	0.530429927414852	1.9368751225249952
9	0.4005068075419834	1.6452656341893745
>10	2.236610402439548	18.748284650068612
>50	0.190052828243783	5.965496961380121
>100	0.09341579693338488	8.49637326014507
>500	0.009663703131039814	3.077337776906489
>1k	0.004294979169351029	2.566653597333856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAA	1388	0.6802587727896491	No Hit
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAA	1383	0.6778082728876691	No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG	1345	0.659184473632621	No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1121	0.5494020780239169	No Hit
TAGAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	800	0.3920799843168006	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	772	0.3783571848657126	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAA	762	0.3734561850617526	No Hit
TAGAGAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	703	0.34454028621838856	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAA	691	0.3386590864536365	No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	687	0.33669868653205254	No Hit
TAGAGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	672	0.3293471868261125	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	603	0.29553028817878846	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	589	0.28866888845324445	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAA	472	0.23132719074691238	No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAA	464	0.22740639090374437	No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAA	432	0.21172319153107233	No Hit
TAGAGAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	403	0.1975102920995883	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGCGGTGGATCAGTCAGCATATTTAGCGACTGACACACCGCCTT	378	0.1852577925896883	No Hit
TAGAGAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	373	0.1828072926877083	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGT	367	0.1798666928053323	No Hit
TAGAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	363	0.1779062928837483	No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA	350	0.17153499313860027	No Hit
TAGAGAGGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAA	345	0.16908449323662025	No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAA	332	0.16271319349147226	No Hit
TAGAGAGGGATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAGTG	308	0.15095079396196823	No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAA	308	0.15095079396196823	No Hit
TAGAGAGGGGTTTCGAGGAAGCTGCGGTCCGTCTTGCGGGTCAGGCGCGGCA	304	0.14899039404038422	No Hit
TAGAGAGGATATGTGAATGTGTATGCCTTTGTGTGTGTGCTTGTGTATGTAC	287	0.14065869437365222	No Hit
TAGAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	283	0.13869829445206822	No Hit
TAGAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	274	0.13428739462850423	No Hit
TAGAGAGGGCGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAA	261	0.1279160948833562	No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGGAGGGGAAATGAGGAAAGGAGATAAA	259	0.1269358949225642	No Hit
TAGAGAGGGAAGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA	256	0.12546559498137622	No Hit
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAA	253	0.1239952950401882	No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAA	246	0.12056459517741619	No Hit
TAGAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	242	0.11860419525583218	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGGCCACCTCTCCTCACCCCTCCCTTCACTTAACAAACTCCAGG	241	0.11811409527543619	No Hit
TAGAGAGGGAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	239	0.11713389531464419	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAAAAAA	226	0.11076259556949619	No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAAAA	225	0.11027249558910018	No Hit
TAGAGAGGGGACAGAGACCGGAAGCTGGGAGATGATGGCTTCTGATGTTTGT	223	0.10929229562830817	No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAA	220	0.10782199568712017	No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	208	0.10194079592236815	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.13575769456969222	0.0	0.0	0.0	0.0
5	0.13575769456969222	0.0	0.0	0.0	0.0
6	0.8635561654577534	0.0	0.0	0.0	0.0
7	0.8689472652421094	0.0	0.0	0.0	0.0
8	0.9145265634189375	0.0	0.0	0.0	0.0
9	0.9561850617525975	0.0	0.0	0.0	0.0
10	1.3614977455400903	0.0	0.0	0.0	0.0
11	2.1696726132130957	0.0	0.0	0.0	0.0
12	2.548029798078808	0.0	0.0	0.0	0.0
13	2.7573024897079006	0.0	0.0	0.0	0.0
14	3.016075279356989	0.0	0.0	0.0	0.0
15	3.2376004704959813	0.0	0.0	0.0	0.0
16	3.4507939619682415	0.0	0.0	0.0	0.0
17	3.634091354636346	0.0	0.0	0.0	0.0
18	3.804156047833758	0.0	0.0	0.0	0.0
19	3.9928445402862183	0.0	0.0	0.0	0.0
20	4.1996667320133305	0.0	0.0	0.0	0.0
21	4.394726524210939	0.0	0.0	0.0	0.0
22	4.587335816506568	0.0	0.0	0.0	0.0
23	4.8461086061556555	0.0	0.0	0.0	0.0
24	5.092138796314448	0.0	0.0	0.0	0.0
25	5.2979807880807686	0.0	0.0	0.0	0.0
26	5.5601842775926285	0.0	0.0	0.0	0.0
27	5.799843168006273	0.0	0.0	0.0	0.0
28	6.10909625563615	0.0	0.0	0.0	0.0
29	6.273769849049206	0.0	0.0	0.0	0.0
30	6.485493040580279	0.0	0.0	0.0	0.0
31	6.622230935110762	0.0	0.0	0.0	0.0
32	6.760929229562831	0.0	0.0	0.0	0.0
33	6.930013722799451	0.0	0.0	0.0	0.0
34	7.101548715938051	0.0	0.0	0.0	0.0
35	7.327974906881003	0.0	0.0	0.0	0.0
36	7.466673201333072	0.0	0.0	0.0	0.0
37	7.604391295824348	0.0	0.0	0.0	0.0
38	7.7573024897079	0.0	0.0	0.0	0.0
39	7.927857282885709	0.0	0.0	0.0	0.0
40	8.111154675553813	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAAACG	35	1.0140502E-7	46.000004	9
GGAGATA	30	1.8538303E-6	46.0	44
GAGGTGG	40	5.5715645E-9	46.0	38
TGTGAAT	30	1.8538303E-6	46.0	12
TAGCGAC	55	1.8189894E-12	46.0	32
GGGTTTC	40	5.5715645E-9	46.0	8
TCCTTCG	25	3.407213E-5	46.0	16
CTGAGTT	25	3.407213E-5	46.0	24
TCCCAGC	20	6.299194E-4	46.0	11
ACGCCGG	25	3.407213E-5	46.0	27
CCGGACG	20	6.299194E-4	46.0	21
ACGCCGC	20	6.299194E-4	46.0	25
TCCCAAA	20	6.299194E-4	46.0	26
CAGCATA	55	1.8189894E-12	46.0	23
TATACTG	20	6.299194E-4	46.0	36
CCTACCT	30	1.8538303E-6	46.0	41
CAGTCAG	55	1.8189894E-12	46.0	19
CTGAGCC	30	1.8538303E-6	46.0	26
ATCCATC	30	1.8538303E-6	46.0	23
CGGAGAT	20	6.299194E-4	46.0	18
>>END_MODULE