FastQCFastQC Report
Fri 17 Jun 2016
SRR1527412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259179
Sequences flagged as poor quality0
Sequence length52
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG25210.9726868303373344No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAA17340.6690356857615779No Hit
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAA16400.6327673152531648No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14420.5563722369482095No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAA8910.3437778523722987No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8780.3387620138977309No Hit
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAA8730.33683284525366636No Hit
TAGAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6680.25773693084702076Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAA5910.22802773372842705No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAA5870.22648439881317547No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAA5550.2141377194911625No Hit
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5440.20989354847422054Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA5340.20603521118609147Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGT5000.19291686440645267No Hit
TAGAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4820.1859718572878204Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4530.17478267915224613Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA4450.1716960093217429No Hit
TAGAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.16899517322005256No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAA4370.16860933949123966No Hit
TAGAGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4300.1659085033895493Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAGTG4210.16243599983023316No Hit
TAGAGAGGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAA4140.15973516372854282No Hit
TAGAGAGGATATGTGAATGTGTATGCCTTTGTGTGTGTGCTTGTGTATGTAC4110.1585776625421041No Hit
TAGAGAGGGGCGGTGGATCAGTCAGCATATTTAGCGACTGACACACCGCCTT4000.15433349152516213No Hit
TAGAGAGGGGTTTCGAGGAAGCTGCGGTCCGTCTTGCGGGTCAGGCGCGGCA3960.15279015660991052No Hit
TAGAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT3830.14777431813534275No Hit
TAGAGAGGGCGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAA3820.14738848440652985No Hit
TAGAGAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3690.14237264593196208Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3520.13581347254214268Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGCAGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAAAA3490.13465597135570395No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGGAG3430.13234096898282655No Hit
TAGAGAGGGGGCCACCTCTCCTCACCCCTCCCTTCACTTAACAAACTCCAGG3350.12925429915232328No Hit
TAGAGAGGGGACAGAGACCGGAAGCTGGGAGATGATGGCTTCTGATGTTTGT3300.12732513050825878No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAA3290.12693929677944585No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAA3260.12578179559300715No Hit
TAGAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190.1230809594913168No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGGAGGGGAAATGAGGAAAGGAGATAAA2900.11189178135574256No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAA2890.11150594762692964No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC2820.10880511152523932Illumina Single End Adapter 2 (100% over 25bp)
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAA2800.1080334440676135No Hit
TAGAGAGGGAAGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA2750.10610427542354897No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC2740.10571844169473606No Hit
TAGAGAGGGCGTACCCAGGCTGGCACTGAGCCATCAGTGTCCTACCTCAGCC2740.10571844169473606No Hit
TAGAGAGGGAAGTCAACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAA2600.10031676949135539No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC206.302363E-446.00000414
TTTGAAA206.302363E-446.00000432
TTTTGAA206.302363E-446.00000431
ACAAACT206.302363E-446.00000441
TTCACGC206.302363E-446.00000429
TTCATCC551.8189894E-1246.00000420
CGGAGTA206.302363E-446.00000444
GGGTTCG206.302363E-446.00000428
AATCCAC206.302363E-446.00000437
GGCATGA206.302363E-446.00000410
CGATAGC206.302363E-446.00000437
ATCCATC551.8189894E-1246.00000423
TAAACAA206.302363E-446.00000443
TGACCTA206.302363E-446.00000428
CGTTAAG206.302363E-446.00000422
TCGACCA206.302363E-446.00000430
CCAGTTT206.302363E-446.00000412
GAAATGT206.302363E-446.00000435
AAATCCA206.302363E-446.00000436
CGCCCGA206.302363E-446.00000433
Produced by FastQC (version 0.11.5)