##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527412_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259179
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.777184879947836	31.0	30.0	33.0	25.0	33.0
2	27.831186168632488	30.0	26.0	31.0	16.0	33.0
3	30.140948919472642	31.0	30.0	31.0	27.0	33.0
4	32.64816593937009	35.0	32.0	35.0	28.0	35.0
5	34.49213863777544	35.0	35.0	35.0	32.0	37.0
6	32.594010317193906	35.0	32.0	35.0	28.0	35.0
7	34.934786383156045	35.0	35.0	35.0	33.0	37.0
8	35.87113153457649	35.0	35.0	37.0	35.0	37.0
9	38.24537867651314	39.0	39.0	39.0	37.0	39.0
10	35.13339429506249	37.0	34.0	39.0	27.0	39.0
11	35.3847958360824	37.0	34.0	39.0	30.0	39.0
12	35.94930530637127	37.0	35.0	39.0	31.0	39.0
13	36.0452428630406	38.0	35.0	39.0	31.0	39.0
14	36.922385687112	39.0	36.0	40.0	32.0	40.0
15	37.18341763800308	39.0	36.0	40.0	32.0	40.0
16	37.07510639365072	39.0	36.0	40.0	32.0	40.0
17	37.31001740110117	39.0	36.0	40.0	32.0	40.0
18	37.27306224655547	39.0	36.0	40.0	32.0	40.0
19	37.016158716562686	39.0	36.0	40.0	32.0	40.0
20	37.244946542736876	39.0	36.0	40.0	32.0	40.0
21	37.21181114210642	39.0	36.0	40.0	32.0	40.0
22	37.23162370408097	39.0	36.0	40.0	32.0	40.0
23	37.001909876957626	39.0	36.0	40.0	32.0	40.0
24	36.774426168786825	39.0	36.0	40.0	32.0	40.0
25	36.13545078883706	37.0	35.0	39.0	31.0	40.0
26	35.7211309558259	37.0	35.0	40.0	30.0	40.0
27	35.53504720675672	37.0	35.0	40.0	30.0	40.0
28	35.29973879056559	37.0	35.0	40.0	30.0	40.0
29	35.09002272560663	37.0	35.0	40.0	29.0	40.0
30	34.7026688119022	36.0	34.0	40.0	25.0	40.0
31	34.439838875834845	36.0	34.0	39.0	25.0	40.0
32	34.05942225257448	36.0	33.0	39.0	23.0	40.0
33	33.84282677223077	35.0	33.0	39.0	22.0	40.0
34	33.41541945913828	35.0	33.0	40.0	19.0	40.0
35	33.080897757920205	35.0	33.0	40.0	17.0	40.0
36	32.413281940280655	35.0	31.0	40.0	12.0	40.0
37	31.579240602054952	35.0	30.0	40.0	10.0	40.0
38	30.86455306950023	35.0	26.0	40.0	9.0	40.0
39	30.04939443396263	35.0	23.0	39.0	8.0	40.0
40	29.34750500619263	35.0	20.0	39.0	8.0	40.0
41	28.97327715594242	35.0	18.0	39.0	8.0	40.0
42	28.51830202292624	35.0	15.0	39.0	8.0	40.0
43	28.32952901276724	35.0	15.0	38.0	8.0	40.0
44	27.894262266618824	35.0	13.0	38.0	8.0	40.0
45	27.590283163373577	35.0	13.0	37.0	8.0	40.0
46	27.199425879411525	34.0	10.0	37.0	8.0	40.0
47	26.982838115742403	34.0	10.0	36.0	8.0	40.0
48	26.692093109395437	33.0	10.0	35.0	8.0	40.0
49	26.377437986873936	33.0	10.0	35.0	8.0	39.0
50	25.851835989798555	33.0	10.0	35.0	8.0	39.0
51	25.50139478892966	33.0	10.0	35.0	8.0	39.0
52	24.810698397632525	32.0	9.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	2.0
12	3.0
13	5.0
14	7.0
15	28.0
16	67.0
17	164.0
18	398.0
19	795.0
20	1342.0
21	2054.0
22	2962.0
23	4048.0
24	5475.0
25	7595.0
26	10717.0
27	14439.0
28	15898.0
29	13387.0
30	9461.0
31	8760.0
32	10173.0
33	13070.0
34	17692.0
35	24815.0
36	39465.0
37	39587.0
38	15944.0
39	824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.22023003406912	0.41515709220268615	0.28474529186392417	0.07986758186427141
8	97.4230165252586	1.6729750481327577	0.533993880677061	0.3700145459315763
9	94.58443778238205	2.858642096774816	1.414852283556924	1.1420678372861999
10	45.451599087889065	36.577809158921056	6.233915556430112	11.736676196759769
11	25.299117598262207	49.68805343025476	14.087561106416802	10.925267865066228
12	40.06728940230497	39.20495101840813	12.328159303029953	8.39960027625695
13	23.3228772392825	43.11383252501167	15.1817855613302	18.381504674375623
14	21.792274837081706	50.44621670737213	12.455870267267025	15.305638188279143
15	27.67199503046157	47.46102114754668	12.508729488114392	12.35825433387736
16	22.275724499284276	48.61852233398539	18.99845280674746	10.107300359982869
17	28.349133224528224	46.32744165229436	13.028447520825376	12.294977602352041
18	26.04724919843043	46.44743594195517	15.39939578438068	12.10591907523372
19	22.02338924064064	45.26215472704193	19.538234193356715	13.17622183896072
20	19.94297377488145	46.84060051161553	17.076229169801564	16.140196543701457
21	19.44601993217043	44.47080974924666	24.100718036569322	11.98245228201359
22	21.13288499454045	47.301671817546946	15.175612221669194	16.38983096624341
23	20.48584183132121	52.15275928991161	14.424008117941653	12.93739076082553
24	22.224794447080974	50.550005980422796	17.73021734013944	9.494982232356788
25	24.2944065684334	48.102662638562535	12.596313744554921	15.006617048449142
26	27.430463116224697	45.46548910212633	14.605735804212532	12.498311977436444
27	25.588878728600694	47.04007654941179	14.822188526076571	12.548856195910934
28	27.148032826733647	49.483561553983925	13.783909961841045	9.584495657441382
29	31.394904679777298	46.1194772724642	14.595318293534584	7.890299754223914
30	26.956659297242446	51.57748120025156	12.513359492860147	8.952500009645844
31	28.551695932154995	50.639133571778586	11.37977999760783	9.429390498458593
32	25.78835476639697	50.47322506838903	15.125068003194702	8.6133521620193
33	26.39179871826035	53.9194919341459	10.49660659235509	9.192102755238658
34	25.775236419617332	51.709822169234386	11.200753147438643	11.314188263709637
35	24.10573387504389	56.239895979226716	10.420597347778948	9.23377279795045
36	27.42236060791962	52.77047908974106	11.006292948116938	8.800867354222373
37	24.66750778419548	51.93669240177638	13.824808337095213	9.57099147693293
38	26.407617901141684	51.951354083471266	11.862457992352775	9.778570023034273
39	24.919071375381492	50.77224620821903	11.583114372692233	12.725568043707245
40	24.141616411823488	51.53619699126858	13.726806569976734	10.595380026931196
41	23.590645847078658	51.38919434059086	14.948356155398393	10.071803656932083
42	22.82708089775792	54.41451660821286	11.326149109302836	11.432253384726385
43	26.40954706978575	52.463741275334804	11.25206903337076	9.874642621508688
44	24.046701314535515	54.218127240247085	10.963079570489894	10.772091874727504
45	25.253203384533467	53.52594153075674	10.523229119643181	10.697625965066614
46	21.91728496521709	55.295375011092716	11.278305726930036	11.509034296760154
47	23.812886074874893	51.8089814375393	11.92149055286115	12.456641934724649
48	22.845986750469752	52.703344020927624	11.95853059082719	12.492138637775437
49	21.81542486081048	52.04819834940331	12.345907654555347	13.790469135230863
50	18.720266688273355	53.27939377804529	14.13116031777266	13.869179215908698
51	17.942425890986538	52.9209542439781	14.062867747772776	15.073752117262588
52	20.1208431238642	49.18106791059461	15.694172753193739	15.003916212347452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	10.5
4	21.0
5	34.0
6	47.0
7	71.0
8	95.0
9	957.5
10	1820.0
11	1691.0
12	1562.0
13	1417.0
14	1146.5
15	1021.0
16	1231.5
17	1442.0
18	1755.0
19	2068.0
20	2560.5
21	3053.0
22	4259.5
23	5466.0
24	6389.5
25	7313.0
26	8477.0
27	9641.0
28	11226.0
29	12811.0
30	15160.0
31	17509.0
32	17767.5
33	18026.0
34	16930.5
35	15835.0
36	14885.0
37	13935.0
38	14972.0
39	16729.5
40	17450.0
41	16295.0
42	15140.0
43	14998.5
44	14857.0
45	13298.0
46	11739.0
47	10507.0
48	9275.0
49	8847.5
50	8420.0
51	11039.0
52	13658.0
53	13133.5
54	12609.0
55	11182.5
56	9756.0
57	7977.5
58	6199.0
59	5057.5
60	3916.0
61	3496.5
62	3077.0
63	2405.0
64	1573.5
65	1414.0
66	942.5
67	471.0
68	312.5
69	154.0
70	125.5
71	97.0
72	161.5
73	226.0
74	128.0
75	30.0
76	18.5
77	7.0
78	5.0
79	3.0
80	2.5
81	2.0
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	259179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.361809517248645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.17901776537782	34.38890087374279
2	8.155917919942334	6.90998882725824
3	2.917922141176456	3.708253857920381
4	1.5459546592742341	2.6195774719391256
5	1.0123200317123286	2.1441854176946387
6	0.7420376902145763	1.8860435572493426
7	0.6025484496725895	1.7867529854960607
8	0.4631081871346576	1.5694480647421367
9	0.4212775859079407	1.6061472763306681
>10	2.5943903062496516	20.19094414500825
>50	0.23105509834951918	6.8144118819876285
>100	0.12150311206310921	10.697512857830505
>500	0.00896334433252445	2.5824055924743856
>1k	0.003983708592233089	3.0954271903258563
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG	2521	0.9726868303373344	No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAA	1734	0.6690356857615779	No Hit
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAA	1640	0.6327673152531648	No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1442	0.5563722369482095	No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAA	891	0.3437778523722987	No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	878	0.3387620138977309	No Hit
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAA	873	0.33683284525366636	No Hit
TAGAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	668	0.25773693084702076	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAA	591	0.22802773372842705	No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAA	587	0.22648439881317547	No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAAA	555	0.2141377194911625	No Hit
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	544	0.20989354847422054	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	534	0.20603521118609147	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGT	500	0.19291686440645267	No Hit
TAGAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	482	0.1859718572878204	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	453	0.17478267915224613	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA	445	0.1716960093217429	No Hit
TAGAGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	438	0.16899517322005256	No Hit
TAGAGAGGGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAA	437	0.16860933949123966	No Hit
TAGAGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	430	0.1659085033895493	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAGTG	421	0.16243599983023316	No Hit
TAGAGAGGGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAAA	414	0.15973516372854282	No Hit
TAGAGAGGATATGTGAATGTGTATGCCTTTGTGTGTGTGCTTGTGTATGTAC	411	0.1585776625421041	No Hit
TAGAGAGGGGCGGTGGATCAGTCAGCATATTTAGCGACTGACACACCGCCTT	400	0.15433349152516213	No Hit
TAGAGAGGGGTTTCGAGGAAGCTGCGGTCCGTCTTGCGGGTCAGGCGCGGCA	396	0.15279015660991052	No Hit
TAGAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	383	0.14777431813534275	No Hit
TAGAGAGGGCGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAAAA	382	0.14738848440652985	No Hit
TAGAGAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	369	0.14237264593196208	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	352	0.13581347254214268	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGCAGAGTGCAGTGCTTAAGAGGGAAAAAAAAAAAAAAAAAAAAA	349	0.13465597135570395	No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGGAG	343	0.13234096898282655	No Hit
TAGAGAGGGGGCCACCTCTCCTCACCCCTCCCTTCACTTAACAAACTCCAGG	335	0.12925429915232328	No Hit
TAGAGAGGGGACAGAGACCGGAAGCTGGGAGATGATGGCTTCTGATGTTTGT	330	0.12732513050825878	No Hit
TAGAGAGGGGTGGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAA	329	0.12693929677944585	No Hit
TAGAGAGGGGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAA	326	0.12578179559300715	No Hit
TAGAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	319	0.1230809594913168	No Hit
TAGAGAGGGCAGAGTGCAGTGCTTAGGAGGGGAAATGAGGAAAGGAGATAAA	290	0.11189178135574256	No Hit
TAGAGAGGGAAGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAA	289	0.11150594762692964	No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	282	0.10880511152523932	Illumina Single End Adapter 2 (100% over 25bp)
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGGAAAAAAAAAAAAAAAA	280	0.1080334440676135	No Hit
TAGAGAGGGAAGGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAAA	275	0.10610427542354897	No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	274	0.10571844169473606	No Hit
TAGAGAGGGCGTACCCAGGCTGGCACTGAGCCATCAGTGTCCTACCTCAGCC	274	0.10571844169473606	No Hit
TAGAGAGGGAAGTCAACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAA	260	0.10031676949135539	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.10957677898286512	0.0	0.0	0.0	0.0
5	0.10957677898286512	0.0	0.0	0.0	0.0
6	0.774368293727501	0.0	0.0	0.0	0.0
7	0.7809274671173204	0.0	0.0	0.0	0.0
8	0.815266668981669	0.0	0.0	0.0	0.0
9	0.8469050347443273	0.0	0.0	0.0	0.0
10	1.2049587350827036	0.0	0.0	0.0	0.0
11	1.866663579996836	0.0	0.0	0.0	0.0
12	2.1822755701657925	0.0	0.0	0.0	0.0
13	2.3921691186400134	0.0	0.0	0.0	0.0
14	2.6070785055888015	0.0	0.0	0.0	0.0
15	2.821216225079964	0.0	0.0	0.0	0.0
16	2.992140566944081	0.0	0.0	0.0	0.0
17	3.1414582199946754	0.0	0.0	0.0	0.0
18	3.298492547621528	0.0	0.0	0.0	0.0
19	3.4593852125365094	0.0	0.0	0.0	0.0
20	3.6387978964345105	0.0	0.0	0.0	0.0
21	3.8135805755867564	0.0	0.0	0.0	0.0
22	4.019229953044035	0.0	0.0	0.0	0.0
23	4.272336879145301	0.0	0.0	0.0	0.0
24	4.522357135416064	0.0	0.0	0.0	0.0
25	4.759644878636	0.0	0.0	0.0	0.0
26	5.0401459994829825	0.0	0.0	0.0	0.0
27	5.281292079991049	0.0	0.0	0.0	0.0
28	5.612337419312522	0.0	0.0	0.0	0.0
29	5.775159252871568	0.0	0.0	0.0	0.0
30	5.9769502930407175	0.0	0.0	0.0	0.0
31	6.134756288125195	0.0	0.0	0.0	0.0
32	6.276357266599532	0.0	0.0	0.0	0.0
33	6.433005760497571	0.0	0.0	0.0	0.0
34	6.590425921853236	0.0	0.0	0.0	0.0
35	6.840060344395186	0.0	0.0	0.0	0.0
36	6.967385474903445	0.0	0.0	0.0	0.0
37	7.1074431184625295	0.0	0.0	0.0	0.0
38	7.270650785750389	0.0	0.0	0.0	0.0
39	7.4300001157501185	0.0	0.0	0.0	0.0
40	7.631405322190456	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTGC	20	6.302363E-4	46.000004	14
TTTGAAA	20	6.302363E-4	46.000004	32
TTTTGAA	20	6.302363E-4	46.000004	31
ACAAACT	20	6.302363E-4	46.000004	41
TTCACGC	20	6.302363E-4	46.000004	29
TTCATCC	55	1.8189894E-12	46.000004	20
CGGAGTA	20	6.302363E-4	46.000004	44
GGGTTCG	20	6.302363E-4	46.000004	28
AATCCAC	20	6.302363E-4	46.000004	37
GGCATGA	20	6.302363E-4	46.000004	10
CGATAGC	20	6.302363E-4	46.000004	37
ATCCATC	55	1.8189894E-12	46.000004	23
TAAACAA	20	6.302363E-4	46.000004	43
TGACCTA	20	6.302363E-4	46.000004	28
CGTTAAG	20	6.302363E-4	46.000004	22
TCGACCA	20	6.302363E-4	46.000004	30
CCAGTTT	20	6.302363E-4	46.000004	12
GAAATGT	20	6.302363E-4	46.000004	35
AAATCCA	20	6.302363E-4	46.000004	36
CGCCCGA	20	6.302363E-4	46.000004	33
>>END_MODULE