FastQCFastQC Report
Fri 17 Jun 2016
SRR1527411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264665
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC126020.5564619932749436No Hit
CCAGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC81080.3580220474109857No Hit
CCAGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT71160.3142186592719011No Hit
CCAGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA70160.30980299514497733No Hit
CCAGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT64160.2833090103834342No Hit
CCAGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC54510.24069785155861906No Hit
CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC54500.2406536949173498No Hit
CCAGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT53440.23597309094281052No Hit
CCAGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA52980.23394188544442557No Hit
CCAGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC48940.2161026023716532No Hit
CCAGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG47740.21080380541934457No Hit
CCAGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG47480.20965573274634436No Hit
CCAGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT47290.20881675656222884No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG45550.20113350098138133No Hit
CCAGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC43450.19186060631484128No Hit
CCAGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA40040.17680319164203095No Hit
CCAGACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC39320.17362391347064576No Hit
CCAGACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT33670.14867541115352603No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT32510.14355324076629436No Hit
CCAGACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT32310.1426701079409096No Hit
CCAGACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA32170.14205191496314024No Hit
CCAGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC31340.13838691373779347No Hit
CCAGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG29650.13092444136329215No Hit
CCAGACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC29100.12849582609348403No Hit
CCAGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA28360.12522823463956037No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG27870.12306455921736768No Hit
CCAGACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC26700.11789823218886679No Hit
CCAGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA25420.11224618210640426No Hit
CCAGACGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT23320.10297328743986418No Hit
CCAGACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC22650.1000147924748252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC254.4442124E-544.032
TACGCTA502.7284841E-1144.041
CCCGTAA254.4442124E-544.016
TATCGCG302.52881E-644.011
CGATAAG302.52881E-644.022
ACGGGAG302900.041.8936965
GACGGGA808500.041.6925164
CAGACGG2364550.041.6749042
CCAGACG2378850.041.6713141
AGACGGG2265700.041.609393
ACGGGAC140150.041.5354965
ACGGGTA24050.041.5301485
ACGGGGC290200.041.4906965
ACGGGAT228750.041.4128955
ACGGGGG390650.041.34755
GACGGGG1173350.041.341294
ACGGGGA305050.041.309955
CGGGATA30500.041.2590186
GACGGGT111300.041.23274
ACGGGCT92200.041.23215
Produced by FastQC (version 0.11.5)