##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2264665
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.549171731801394	31.0	31.0	33.0	30.0	34.0
2	32.159405916548366	34.0	31.0	34.0	30.0	34.0
3	32.61162158641565	34.0	31.0	34.0	31.0	34.0
4	36.20658154738118	37.0	37.0	37.0	35.0	37.0
5	34.941631543738254	35.0	35.0	37.0	33.0	37.0
6	35.85822759657609	37.0	35.0	37.0	35.0	37.0
7	36.19959905769728	37.0	35.0	37.0	35.0	37.0
8	36.5614980582117	37.0	37.0	37.0	35.0	37.0
9	38.46485241746572	39.0	39.0	39.0	37.0	39.0
10	36.819276140179674	39.0	37.0	39.0	32.0	39.0
11	36.892324913397786	38.0	37.0	39.0	34.0	39.0
12	36.81090757352633	38.0	37.0	39.0	33.0	39.0
13	36.934853057737016	39.0	37.0	39.0	33.0	39.0
14	37.78496952087836	40.0	38.0	40.0	33.0	40.0
15	37.76241784104934	40.0	37.0	40.0	33.0	40.0
16	37.92184009555497	40.0	38.0	40.0	34.0	40.0
17	37.81124228086715	40.0	37.0	40.0	34.0	40.0
18	37.66676572473192	39.0	37.0	40.0	33.0	40.0
19	37.566217520030555	39.0	37.0	40.0	33.0	40.0
20	37.39349970083875	39.0	36.0	40.0	33.0	40.0
21	37.26256112935026	39.0	36.0	40.0	33.0	40.0
22	37.00763159231056	38.0	36.0	40.0	33.0	40.0
23	36.752932994504704	38.0	35.0	40.0	32.0	40.0
24	36.62425656774843	38.0	35.0	40.0	32.0	40.0
25	36.412794828374174	38.0	35.0	40.0	31.0	40.0
26	35.817144699105604	38.0	35.0	39.0	30.0	40.0
27	35.69452612196506	38.0	34.0	39.0	30.0	40.0
28	35.495562036769236	37.0	34.0	39.0	30.0	40.0
29	35.09765285373334	37.0	34.0	38.0	30.0	40.0
30	34.804591849125586	36.0	33.0	38.0	29.0	40.0
31	34.68366138038076	36.0	33.0	38.0	29.0	39.0
32	34.584546058688595	36.0	33.0	38.0	29.0	39.0
33	34.0292409694149	35.0	33.0	38.0	27.0	39.0
34	33.60217559771534	35.0	32.0	38.0	27.0	39.0
35	33.425319859670196	35.0	32.0	38.0	26.0	39.0
36	33.02832516067498	34.0	31.0	37.0	26.0	38.0
37	32.79616587883859	34.0	31.0	37.0	26.0	38.0
38	32.353699995363556	34.0	30.0	37.0	24.0	38.0
39	31.469122806242865	33.0	29.0	36.0	23.0	38.0
40	31.179926390879004	33.0	29.0	36.0	23.0	38.0
41	30.892508163459055	33.0	29.0	35.0	22.0	38.0
42	30.483476805620256	32.0	28.0	35.0	22.0	37.0
43	29.979147467727014	31.0	27.0	34.0	21.0	37.0
44	29.540030423925835	31.0	27.0	34.0	21.0	37.0
45	29.05076291636953	31.0	26.0	34.0	20.0	36.0
46	28.851598801588757	31.0	26.0	34.0	20.0	36.0
47	28.3041990758015	30.0	25.0	33.0	19.0	36.0
48	27.716704236608948	29.0	24.0	33.0	18.0	35.0
49	27.186073436910096	29.0	24.0	32.0	17.0	35.0
50	30.957081952518365	32.0	29.0	35.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	10.0
13	12.0
14	37.0
15	105.0
16	206.0
17	388.0
18	648.0
19	1104.0
20	1834.0
21	3164.0
22	4991.0
23	7628.0
24	11145.0
25	15120.0
26	21368.0
27	28755.0
28	39264.0
29	52704.0
30	71157.0
31	99084.0
32	151475.0
33	226872.0
34	338247.0
35	582747.0
36	533651.0
37	71560.0
38	1386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.49250772189264	0.35687397473798554	0.08054171367509103	0.07007658969428149
8	99.28916638884779	0.5108923394850894	0.1122903387476735	0.08765093291943842
9	95.60120371004099	2.679424992217392	0.627465872435879	1.0919054253057294
10	50.796298790328805	35.71464212146167	5.4589089335508785	8.030150154658635
11	33.49091366714283	22.49529179812467	22.941847911280476	21.071946623452035
12	26.129162591376648	17.944464192275678	28.382211055498274	27.544162160849396
13	23.708583830279533	17.809477339915617	29.192264639582454	29.289674190222392
14	24.487683608833976	18.91745578264335	28.84060114851424	27.754259460008434
15	25.805803507362018	20.8876809594355	25.58131114314921	27.725204390053275
16	30.906072200524136	20.38491344194395	24.276747333490825	24.43226702404108
17	28.92070129577664	22.031117185102435	22.375848083491377	26.672333435629554
18	28.98777523386461	19.419914203646012	23.05122391170438	28.541086650784997
19	31.559987901080294	20.23142495689208	22.28060220827363	25.927984933754
20	31.342472286188023	20.463070696990503	22.81414690472984	25.380310112091635
21	31.66918727493912	19.40565160851605	24.388816889032153	24.53634422751268
22	30.240719929879255	19.31301097513319	24.373715317718073	26.072553777269487
23	28.03337358947129	22.252430271143854	24.130809634095993	25.583386505288864
24	28.2710687894236	20.842376245493263	24.40577303927954	26.480781925803598
25	27.05813001039889	21.482426760690878	23.483517429730224	27.97592579918001
26	26.732253997831908	21.282662115588842	23.16037912892194	28.824704757657315
27	28.05730648903922	19.279054517997142	22.88024939670989	29.783389596253752
28	28.950595341915914	21.8022091567627	22.251679608242277	26.99551589307911
29	28.893015081700824	20.44818990888277	23.05868638407888	27.600108625337523
30	28.25150739734133	21.063910114741034	23.044467945590185	27.640114542327453
31	30.951686010955264	21.04081619135722	21.724846721258995	26.28265107642852
32	31.476178595951275	20.239108212472924	22.849604687669036	25.43510850390676
33	31.24925761646866	20.499499926037625	23.076569823792923	25.17467263370079
34	27.383078733499218	23.390126133445786	25.587669699491975	23.63912543356302
35	29.07361574449201	23.380235045801477	24.297810051376253	23.24833915833026
36	29.30618877405709	23.21937240165764	21.849898329333477	25.62454049495179
37	28.918979186767135	23.949811561533384	22.27830606292763	24.85290318877185
38	30.474661815323678	23.21142420622918	22.541170548403404	23.77274343004374
39	29.788467609999714	21.58297143286093	21.953224869903494	26.675336087235863
40	27.522966973040162	22.732722058229363	23.12726164797001	26.617049320760465
41	27.147017329273865	21.930660826214915	22.622992804675306	28.299329039835914
42	25.914517158166884	19.924801239918487	24.488964151430785	29.671717450483847
43	25.8689916610183	20.117986545471407	26.110572645402303	27.902449148107998
44	26.44006950255336	20.606800564321876	24.90840808684728	28.044721846277486
45	27.398312774737104	20.533897949586365	24.057862862719208	28.009926412957327
46	26.646678427052123	22.088476662111173	23.932281374949497	27.332563535887207
47	27.512943415472048	22.45109100021416	24.67133107987274	25.364634504441053
48	27.496870398050042	22.05730207337509	25.021978968191767	25.423848560383107
49	27.17183336166718	22.09081696409844	23.62698235721398	27.1103673170204
50	25.283916164200885	22.80425581708553	24.791790397255223	27.12003762145836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	19.0
8	38.0
9	35.0
10	32.0
11	52.5
12	73.0
13	342.5
14	612.0
15	1078.0
16	1544.0
17	2322.0
18	3100.0
19	2726.5
20	2353.0
21	2392.0
22	2431.0
23	2709.0
24	2987.0
25	3346.5
26	3706.0
27	5339.5
28	6973.0
29	7200.0
30	7427.0
31	9682.5
32	11938.0
33	13739.0
34	15540.0
35	18372.5
36	21205.0
37	26356.0
38	31507.0
39	33435.5
40	35364.0
41	40176.5
42	44989.0
43	46931.0
44	48873.0
45	54636.5
46	60400.0
47	62489.5
48	64579.0
49	76651.0
50	88723.0
51	95962.0
52	103201.0
53	111339.5
54	119478.0
55	127543.0
56	135608.0
57	146204.5
58	156801.0
59	179078.5
60	201356.0
61	203890.5
62	206425.0
63	209237.5
64	212050.0
65	208124.0
66	204198.0
67	174940.0
68	145682.0
69	124434.0
70	103186.0
71	88524.0
72	73862.0
73	64695.5
74	55529.0
75	46673.5
76	37818.0
77	36637.5
78	35457.0
79	24631.5
80	13806.0
81	8975.0
82	4144.0
83	2673.5
84	1203.0
85	782.5
86	362.0
87	216.5
88	71.0
89	48.0
90	25.0
91	15.0
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2264665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.629901124391784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.75933256872986	7.415348077091618
2	10.019791301761117	2.1301878164952304
3	3.732973009886657	1.1904340198535506
4	1.6696271593936052	0.7099188647581257
5	0.9154294882445035	0.4865462473195823
6	0.5786679195151947	0.36907096609824136
7	0.43020539185457196	0.3201128545016026
8	0.3468835595006165	0.29498703513349017
9	0.2992721725059695	0.2863110242718339
>10	7.737623413232803	23.030232164674334
>50	3.043392445059514	22.155377702064182
>100	1.3671078587684828	27.586314601973683
>500	0.06730377150860221	4.694935935050778
>1k	0.028604102891155937	6.522105663178743
>5k	0.00336518857543011	2.2446245547878436
>10k+	4.2064857192876376E-4	0.5634924727471944
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	12602	0.5564619932749436	No Hit
CCAGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	8108	0.3580220474109857	No Hit
CCAGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7116	0.3142186592719011	No Hit
CCAGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	7016	0.30980299514497733	No Hit
CCAGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	6416	0.2833090103834342	No Hit
CCAGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	5451	0.24069785155861906	No Hit
CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	5450	0.2406536949173498	No Hit
CCAGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5344	0.23597309094281052	No Hit
CCAGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	5298	0.23394188544442557	No Hit
CCAGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4894	0.2161026023716532	No Hit
CCAGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	4774	0.21080380541934457	No Hit
CCAGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	4748	0.20965573274634436	No Hit
CCAGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	4729	0.20881675656222884	No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4555	0.20113350098138133	No Hit
CCAGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	4345	0.19186060631484128	No Hit
CCAGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4004	0.17680319164203095	No Hit
CCAGACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	3932	0.17362391347064576	No Hit
CCAGACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	3367	0.14867541115352603	No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	3251	0.14355324076629436	No Hit
CCAGACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT	3231	0.1426701079409096	No Hit
CCAGACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA	3217	0.14205191496314024	No Hit
CCAGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	3134	0.13838691373779347	No Hit
CCAGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2965	0.13092444136329215	No Hit
CCAGACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	2910	0.12849582609348403	No Hit
CCAGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2836	0.12522823463956037	No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	2787	0.12306455921736768	No Hit
CCAGACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	2670	0.11789823218886679	No Hit
CCAGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2542	0.11224618210640426	No Hit
CCAGACGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT	2332	0.10297328743986418	No Hit
CCAGACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	2265	0.1000147924748252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0011039160317309624	0.0	0.0	0.0	0.0
8	0.0012805425968079164	0.0	0.0	0.0	0.0
9	0.001677952368231063	0.0	0.0	0.0	0.0
10	0.009449521231617039	0.0	0.0	0.0	0.0
11	0.01792759635531083	0.0	0.0	0.0	0.0
12	0.025743321859966042	0.0	0.0	0.0	0.0
13	0.0333824207995443	0.0	0.0	0.0	0.0
14	0.04115398966293028	0.0	0.0	0.0	0.0
15	0.050603510894547316	0.0	0.0	0.0	0.0
16	0.05841923639920253	0.0	0.0	0.0	0.0
17	0.06499857594831907	0.0	0.0	0.0	0.0
18	0.07259351824662809	0.0	0.0	0.0	0.0
19	0.08146900314174503	0.0	0.0	0.0	0.0
20	0.08831328253847699	0.0	0.0	0.0	0.0
21	0.09427442910982418	0.0	0.0	0.0	0.0
22	0.09988232255101748	0.0	0.0	0.0	0.0
23	0.10778636133821116	0.0	0.0	0.0	0.0
24	0.11533714699525095	0.0	0.0	0.0	0.0
25	0.12275546272848302	0.0	0.0	0.0	0.0
26	0.12999715189663813	0.0	0.0	0.0	0.0
27	0.13909341999810126	0.0	0.0	0.0	0.0
28	0.15406252138837312	0.0	0.0	0.0	0.0
29	0.16245228322952843	0.0	0.0	0.0	0.0
30	0.172740780645261	0.0	0.0	0.0	0.0
31	0.1819253620292626	0.0	0.0	0.0	0.0
32	0.19124241333707193	0.0	0.0	0.0	0.0
33	0.198660729070304	0.0	0.0	0.0	0.0
34	0.20837519014953645	0.0	0.0	0.0	0.0
35	0.22312350833346212	0.0	0.0	0.0	0.0
36	0.2353548979650412	0.0	0.0	0.0	0.0
37	0.24471610591411974	0.0	0.0	0.0	0.0
38	0.25323833767908277	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAC	25	4.4442124E-5	44.0	32
TACGCTA	50	2.7284841E-11	44.0	41
CCCGTAA	25	4.4442124E-5	44.0	16
TATCGCG	30	2.52881E-6	44.0	11
CGATAAG	30	2.52881E-6	44.0	22
ACGGGAG	30290	0.0	41.893696	5
GACGGGA	80850	0.0	41.692516	4
CAGACGG	236455	0.0	41.674904	2
CCAGACG	237885	0.0	41.671314	1
AGACGGG	226570	0.0	41.60939	3
ACGGGAC	14015	0.0	41.535496	5
ACGGGTA	2405	0.0	41.530148	5
ACGGGGC	29020	0.0	41.490696	5
ACGGGAT	22875	0.0	41.412895	5
ACGGGGG	39065	0.0	41.3475	5
GACGGGG	117335	0.0	41.34129	4
ACGGGGA	30505	0.0	41.30995	5
CGGGATA	3050	0.0	41.259018	6
GACGGGT	11130	0.0	41.2327	4
ACGGGCT	9220	0.0	41.2321	5
>>END_MODULE