FastQCFastQC Report
Fri 17 Jun 2016
SRR1527410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824570
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC92090.5047216604460228No Hit
CCAGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC68010.37274535918051926No Hit
CCAGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG59340.32522731383284825No Hit
CCAGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT52730.2889995999057312No Hit
CCAGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC51290.2811073293981595No Hit
CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA49990.27398236296771294No Hit
CCAGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC48460.26559682555341807No Hit
CCAGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG45110.24723633513649793No Hit
CCAGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA44750.245263267509605No Hit
CCAGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG41020.22482009459763122No Hit
CCAGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT40640.2227374121025776No Hit
CCAGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT39330.2155576382380506No Hit
CCAGACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC37430.20514422576278246No Hit
CCAGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC35760.19599138427136256No Hit
CCAGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA35640.19533369506239828No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT35000.1918260192812553No Hit
CCAGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG33330.1826731777898354No Hit
CCAGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT29310.16064058928953123No Hit
CCAGACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG27060.14830891662145054No Hit
CCAGACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT26820.14699353820352193No Hit
CCAGACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG25440.13943011230043242No Hit
CCAGACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA24400.13373013915607512No Hit
CCAGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG23900.1309897674520572No Hit
CCAGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC23740.13011284850677146No Hit
CCAGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT23720.13000323363861074No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT23640.12956477416596787No Hit
CCAGACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT21710.11898693938845865No Hit
CCAGACGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC20640.11312254394186026No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT20540.11257446960105669No Hit
CCAGACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT18700.10248990173027069No Hit
CCAGACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG18410.1009004861419403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG206.3124334E-446.00000411
ATACTCG301.8620085E-646.044
ACGGGAG246700.043.69725
ACGGGTA21500.043.646515
CAGACGG1903100.043.6203542
CCAGACG1915100.043.602841
AGACGGG1829300.043.557043
GACGGGA659250.043.547364
ACGGGGG311400.043.4961435
CGGGATA22700.043.466966
CGGGAGT74500.043.4375846
ACGGGGC234050.043.415515
GACGGGG943700.043.3946154
ACGGGAC116950.043.3253565
ACGGGAT186200.043.2207265
ACGGGGA247600.043.1296425
AGACGGA49500.042.7939383
GACGGGT91450.042.75564
ACGGGGT153850.042.6811835
ACGGGAA112850.042.6575135
Produced by FastQC (version 0.11.5)