##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527410_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1824570
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.153325441062826	33.0	31.0	34.0	31.0	34.0
2	32.76603035235699	34.0	31.0	34.0	31.0	34.0
3	32.99570145294508	34.0	31.0	34.0	31.0	34.0
4	36.46706456863809	37.0	37.0	37.0	35.0	37.0
5	35.64915075880892	37.0	35.0	37.0	35.0	37.0
6	36.32909671867892	37.0	35.0	37.0	35.0	37.0
7	36.46744767260231	37.0	37.0	37.0	35.0	37.0
8	36.66255501296196	37.0	37.0	37.0	35.0	37.0
9	38.61472675753739	39.0	39.0	39.0	39.0	39.0
10	37.35487539529862	39.0	37.0	39.0	34.0	39.0
11	37.58542012638594	39.0	37.0	39.0	35.0	39.0
12	37.52287443068778	39.0	37.0	39.0	35.0	39.0
13	37.53660862559398	39.0	37.0	39.0	35.0	39.0
14	38.335969022838256	40.0	38.0	40.0	35.0	40.0
15	38.347507631935194	40.0	38.0	40.0	35.0	40.0
16	38.46425459149279	40.0	38.0	40.0	35.0	40.0
17	38.370280120795584	40.0	38.0	40.0	35.0	40.0
18	38.27980400861573	40.0	38.0	40.0	35.0	40.0
19	38.16622985141704	40.0	38.0	40.0	34.0	40.0
20	38.121392985744585	40.0	38.0	40.0	34.0	40.0
21	38.071882690168096	40.0	38.0	40.0	34.0	40.0
22	37.985860778155946	40.0	38.0	40.0	34.0	40.0
23	37.763206673353174	40.0	37.0	40.0	34.0	40.0
24	37.74227955079827	40.0	37.0	40.0	34.0	40.0
25	37.771878853647706	40.0	37.0	40.0	34.0	40.0
26	37.72496643044663	40.0	37.0	40.0	34.0	40.0
27	37.6637026806316	40.0	37.0	40.0	34.0	40.0
28	37.507168812377714	39.0	37.0	40.0	33.0	40.0
29	37.4295477838614	39.0	36.0	40.0	33.0	40.0
30	37.26942128830355	39.0	36.0	40.0	33.0	40.0
31	37.255331941224505	39.0	36.0	40.0	33.0	40.0
32	37.106824621691686	39.0	36.0	40.0	33.0	40.0
33	36.84895893278964	39.0	35.0	40.0	32.0	40.0
34	36.457545613487014	38.0	35.0	40.0	31.0	40.0
35	36.4110491787106	38.0	35.0	40.0	31.0	40.0
36	36.296913245312595	38.0	35.0	40.0	31.0	40.0
37	36.115389927489765	38.0	35.0	40.0	30.0	40.0
38	36.25762453619209	38.0	35.0	40.0	31.0	40.0
39	36.182933513101716	38.0	35.0	40.0	31.0	40.0
40	35.9347150287465	38.0	35.0	40.0	30.0	40.0
41	35.83638775163463	38.0	34.0	40.0	30.0	40.0
42	35.72665340326762	38.0	34.0	40.0	30.0	40.0
43	35.6384665976093	37.0	34.0	40.0	30.0	40.0
44	35.49198222046839	37.0	34.0	40.0	30.0	40.0
45	35.352021024131716	37.0	34.0	40.0	29.0	40.0
46	35.138839836235384	36.0	34.0	40.0	29.0	40.0
47	34.97728560701974	36.0	34.0	40.0	29.0	40.0
48	34.79154430907008	36.0	33.0	39.0	28.0	40.0
49	34.60016880689697	35.0	33.0	39.0	28.0	40.0
50	35.0386748658588	36.0	34.0	39.0	29.0	40.0
51	35.14896441353305	35.0	34.0	39.0	30.0	40.0
52	34.822549970678025	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	4.0
14	12.0
15	23.0
16	51.0
17	111.0
18	235.0
19	423.0
20	808.0
21	1203.0
22	1942.0
23	2942.0
24	4370.0
25	5963.0
26	8167.0
27	10907.0
28	14313.0
29	18976.0
30	24385.0
31	33797.0
32	47127.0
33	69083.0
34	123964.0
35	152802.0
36	241161.0
37	374182.0
38	580432.0
39	107180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.51462536378435	0.3500550814712508	0.0819371139501362	0.053382440794269334
8	99.30016387422789	0.5041735861052193	0.11147832091944951	0.0841842187474309
9	95.69273856305868	2.6389779509692697	0.6027721600157845	1.0655113259562528
10	50.99831741177373	35.58416503614551	5.441775322404731	7.975742229676033
11	33.544013109938234	22.47219892906274	22.77802441123114	21.20576354976789
12	26.128841316036105	17.9124944507473	28.428835287218355	27.529828945998236
13	23.79985421222535	17.729876080391545	29.241191075157435	29.229078632225676
14	24.624706095134744	18.835396833226458	28.765681777076242	27.774215294562556
15	25.758288254218805	20.880536235935043	25.69569816449903	27.66547734534712
16	30.947839764985723	20.31432063445086	24.230969488701447	24.50687011186197
17	28.886532169223432	22.01247417199669	22.359843689198	26.741149969581873
18	29.00508064913925	19.371577960834607	23.037427996733477	28.585913393292667
19	31.703634280953867	20.092460141293564	22.284757504507912	25.919148073244653
20	31.538389867201587	20.305935097036564	22.84998657217865	25.3056884635832
21	31.783159867804468	19.31704456392465	24.395501405810684	24.504294162460198
22	30.284340968008898	19.236313213524284	24.34732567125405	26.13202014721277
23	28.126901132869662	22.01965394586122	24.24341077623769	25.61003414503143
24	28.390963350268834	20.652756539897073	24.465819343735784	26.490460766098312
25	27.035685120329724	21.37944830836855	23.535134305617213	28.04973226568452
26	26.719775070290535	21.166466619532272	23.300394065451037	28.813364244726152
27	28.180064343927608	19.145113643214565	22.881117194736294	29.79370481812153
28	29.09277254366782	21.59369056818867	22.243213469475	27.070323418668508
29	28.88499756106918	20.326268655080376	23.13295735433554	27.6557764295149
30	28.324646355031597	20.83137396756496	23.142329425563283	27.70165025184016
31	31.01508848660232	20.85598250546704	21.792915591070773	26.33601341685986
32	31.53005913722137	20.11619176025036	22.936143858552974	25.417605243975295
33	31.226864411888826	20.347533939503553	23.22563672536543	25.19996492324219
34	27.501877154617254	23.15203034139551	25.601758222485298	23.744334281501946
35	29.063231336698507	23.173021588648286	24.319209457570825	23.444537617082382
36	29.45455641603227	23.084069123135862	21.85040858942107	25.610965871410794
37	29.0498583227829	23.781329299506186	22.329096718678922	24.83971565903199
38	30.549663756391915	23.115309360561668	22.594090662457454	23.74093622058896
39	29.742076215217832	21.347824418904178	22.114415999386157	26.79568336649183
40	27.66920425086459	22.514345845870533	23.171103328455473	26.64534657480941
41	27.18974881752961	21.813358763982745	22.704472834695295	28.292419583792345
42	26.2496917081833	19.722729190987465	24.554607386945964	29.472971713883272
43	25.93942682385439	19.837221920781335	26.265750286368846	27.957600968995433
44	26.47604641093518	20.483894835495487	25.000794707794167	28.03926404577517
45	27.540077936171265	20.345889716481143	24.11653156634166	27.997500781005936
46	26.76811522714941	21.94845908899083	23.974744734375772	27.30868094948399
47	27.63259288489891	22.201176167535365	24.771370788733783	25.394860158831946
48	27.56419320716662	21.834240396367363	25.226656143639325	25.374910252826695
49	27.347100960774316	21.78738004022866	23.771683191108043	27.093835807888983
50	25.29456255446489	22.85946825827455	24.81357251297566	27.032396674284897
51	26.287673260000986	22.610149240643	24.098116268490656	27.004061230865357
52	27.209917953271184	22.34093512444028	23.75480250141129	26.694344420877247
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	22.5
8	45.0
9	31.0
10	17.0
11	41.5
12	66.0
13	277.5
14	933.5
15	1378.0
16	2037.5
17	2697.0
18	2299.5
19	1902.0
20	1914.0
21	1926.0
22	1918.5
23	1911.0
24	2052.5
25	2194.0
26	3083.5
27	3973.0
28	4620.5
29	5268.0
30	6462.0
31	7656.0
32	9265.5
33	10875.0
34	11760.0
35	12645.0
36	16019.0
37	19393.0
38	21374.0
39	27860.0
40	32365.0
41	33913.0
42	35461.0
43	38015.5
44	40570.0
45	43327.0
46	46084.0
47	49061.0
48	52038.0
49	61904.0
50	71770.0
51	74499.5
52	77229.0
53	83725.5
54	90222.0
55	97627.0
56	105032.0
57	111512.0
58	117992.0
59	134645.0
60	151298.0
61	157970.0
62	164642.0
63	163198.0
64	159971.5
65	158189.0
66	140935.0
67	123681.0
68	109496.5
69	95312.0
70	78826.5
71	62341.0
72	55734.0
73	49127.0
74	42007.0
75	34887.0
76	34354.5
77	33822.0
78	24985.0
79	16148.0
80	10816.5
81	5485.0
82	3855.5
83	2226.0
84	1487.0
85	748.0
86	473.5
87	199.0
88	151.5
89	75.0
90	46.0
91	26.5
92	7.0
93	3.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1824570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.240710213396174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.56515670038688	5.966279029507895
2	10.1044119258056	1.8674388496630783
3	3.7127208257499023	1.0292453176198741
4	1.7690021750054024	0.6538734586436987
5	1.0379052803366484	0.4795490962272343
6	0.651230905708364	0.3610701648995111
7	0.5327815240601378	0.34462957696239066
8	0.46231378945885643	0.3417686204837074
9	0.42248047304976516	0.3513617660044271
>10	11.968559270914787	30.115411212456788
>50	3.1870732249712526	19.945032142556048
>100	1.4847505421658826	25.796753231758107
>500	0.06096801743193238	3.6548953805180364
>1k	0.037656716649134704	7.3060900765897925
>5k	0.0029886283054868816	1.7866020761094195
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9209	0.5047216604460228	No Hit
CCAGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6801	0.37274535918051926	No Hit
CCAGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	5934	0.32522731383284825	No Hit
CCAGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5273	0.2889995999057312	No Hit
CCAGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5129	0.2811073293981595	No Hit
CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4999	0.27398236296771294	No Hit
CCAGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4846	0.26559682555341807	No Hit
CCAGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4511	0.24723633513649793	No Hit
CCAGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4475	0.245263267509605	No Hit
CCAGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4102	0.22482009459763122	No Hit
CCAGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	4064	0.2227374121025776	No Hit
CCAGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3933	0.2155576382380506	No Hit
CCAGACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	3743	0.20514422576278246	No Hit
CCAGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3576	0.19599138427136256	No Hit
CCAGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	3564	0.19533369506239828	No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3500	0.1918260192812553	No Hit
CCAGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	3333	0.1826731777898354	No Hit
CCAGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2931	0.16064058928953123	No Hit
CCAGACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2706	0.14830891662145054	No Hit
CCAGACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	2682	0.14699353820352193	No Hit
CCAGACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	2544	0.13943011230043242	No Hit
CCAGACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA	2440	0.13373013915607512	No Hit
CCAGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2390	0.1309897674520572	No Hit
CCAGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2374	0.13011284850677146	No Hit
CCAGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2372	0.13000323363861074	No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2364	0.12956477416596787	No Hit
CCAGACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	2171	0.11898693938845865	No Hit
CCAGACGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	2064	0.11312254394186026	No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2054	0.11257446960105669	No Hit
CCAGACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1870	0.10248990173027069	No Hit
CCAGACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1841	0.1009004861419403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0018086453246518358	0.0	0.0	0.0	0.0
8	0.002082682495053629	0.0	0.0	0.0	0.0
9	0.0023567196654554223	0.0	0.0	0.0	0.0
10	0.01107110168423245	0.0	0.0	0.0	0.0
11	0.02038836547789342	0.0	0.0	0.0	0.0
12	0.03019889617827762	0.0	0.0	0.0	0.0
13	0.03847481872441178	0.0	0.0	0.0	0.0
14	0.04888823119967992	0.0	0.0	0.0	0.0
15	0.059137221372706994	0.0	0.0	0.0	0.0
16	0.0668102621439572	0.0	0.0	0.0	0.0
17	0.07316792449727881	0.0	0.0	0.0	0.0
18	0.08138903960933261	0.0	0.0	0.0	0.0
19	0.0908159182711543	0.0	0.0	0.0	0.0
20	0.09958510772401169	0.0	0.0	0.0	0.0
21	0.10583315520917257	0.0	0.0	0.0	0.0
22	0.1117523580898513	0.0	0.0	0.0	0.0
23	0.11964462859742296	0.0	0.0	0.0	0.0
24	0.1278109362753964	0.0	0.0	0.0	0.0
25	0.13383975402423584	0.0	0.0	0.0	0.0
26	0.14167721709772713	0.0	0.0	0.0	0.0
27	0.14978871734162022	0.0	0.0	0.0	0.0
28	0.16634056243388853	0.0	0.0	0.0	0.0
29	0.173136684259853	0.0	0.0	0.0	0.0
30	0.1830568298283979	0.0	0.0	0.0	0.0
31	0.191168330072291	0.0	0.0	0.0	0.0
32	0.20043078643187162	0.0	0.0	0.0	0.0
33	0.20739133056007716	0.0	0.0	0.0	0.0
34	0.21774993560126496	0.0	0.0	0.0	0.0
35	0.23139698668727426	0.0	0.0	0.0	0.0
36	0.24208443633294421	0.0	0.0	0.0	0.0
37	0.25112766295620337	0.0	0.0	0.0	0.0
38	0.25951320037049824	0.0	0.0	0.0	0.0
39	0.2678987377847931	0.0	0.0	0.0	0.0
40	0.2751333190834005	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	20	6.3124334E-4	46.000004	11
ATACTCG	30	1.8620085E-6	46.0	44
ACGGGAG	24670	0.0	43.6972	5
ACGGGTA	2150	0.0	43.64651	5
CAGACGG	190310	0.0	43.620354	2
CCAGACG	191510	0.0	43.60284	1
AGACGGG	182930	0.0	43.55704	3
GACGGGA	65925	0.0	43.54736	4
ACGGGGG	31140	0.0	43.496143	5
CGGGATA	2270	0.0	43.46696	6
CGGGAGT	7450	0.0	43.437584	6
ACGGGGC	23405	0.0	43.41551	5
GACGGGG	94370	0.0	43.394615	4
ACGGGAC	11695	0.0	43.325356	5
ACGGGAT	18620	0.0	43.220726	5
ACGGGGA	24760	0.0	43.129642	5
AGACGGA	4950	0.0	42.793938	3
GACGGGT	9145	0.0	42.7556	4
ACGGGGT	15385	0.0	42.681183	5
ACGGGAA	11285	0.0	42.657513	5
>>END_MODULE