SRR1527409_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2528012
Sequences flagged as poor quality	0
Sequence length	52
%GC	58

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	12435	0.4918884878711019	No Hit
CCAGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	9262	0.3663748431573901	No Hit
CCAGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	7966	0.31510926372184944	No Hit
CCAGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	7096	0.2806948701192874	No Hit
CCAGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	6892	0.2726252881711005	No Hit
CCAGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6750	0.2670082262267742	No Hit
CCAGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6467	0.25581365911237763	No Hit
CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6401	0.2532029120114936	No Hit
CCAGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	6127	0.24236435586539937	No Hit
CCAGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	5841	0.2310511184282353	No Hit
CCAGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5588	0.22104325454151325	No Hit
CCAGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5519	0.21831383711786176	No Hit
CCAGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	5343	0.21135184484883776	No Hit
CCAGACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	4912	0.19430287514458003	No Hit
CCAGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	4767	0.18856714287748635	No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4589	0.18152603705995066	No Hit
CCAGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	4508	0.17832193834522939	No Hit
CCAGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3809	0.1506717531404123	No Hit
CCAGACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	3779	0.14948504991273776	No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	3688	0.14588538345545826	No Hit
CCAGACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA	3602	0.1424835008694579	No Hit
CCAGACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3529	0.13959585634878316	No Hit
CCAGACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	3477	0.1375389040874806	No Hit
CCAGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3335	0.1319218421431544	No Hit
CCAGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3212	0.12705635890968872	No Hit
CCAGACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	3033	0.11997569631789723	No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3014	0.11922411760703668	No Hit
CCAGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2773	0.10969093501138445	No Hit
CCAGACGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	2755	0.10897891307477969	No Hit
CCAGACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	2666	0.10545836016601186	No Hit
CCAGACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2619	0.10359919177598842	No Hit
CCAGACGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT	2549	0.10083021757808111	No Hit
CCAGACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	2544	0.10063243370680203	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACG	45	3.110472E-10	46.000004	11
GCGTAAC	30	1.8622959E-6	46.0	31
CGGGATA	3195	0.0	43.912365	6
ACGGGAG	33570	0.0	43.75961	5
GACGGGA	91115	0.0	43.59183	4
ACGGGAT	25725	0.0	43.550243	5
ACGGGTA	2590	0.0	43.51351	5
CAGACGG	264645	0.0	43.461395	2
CCAGACG	266390	0.0	43.451256	1
AGACGGG	253445	0.0	43.410007	3
ACGGGAC	16010	0.0	43.18426	5
ACGGGGG	43980	0.0	43.102776	5
GACGGGG	131010	0.0	43.050606	4
ACGGGGA	34070	0.0	43.00939	5
ACGGGGC	32130	0.0	42.950516	5
ACGGGAA	16155	0.0	42.78242	5
AGACGGA	7295	0.0	42.75257	3
CGGGAGT	10140	0.0	42.733727	6
GACGGGC	20515	0.0	42.61418	4
ACGGGCT	10375	0.0	42.49735	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content