##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2528012
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.064058240229873	31.0	31.0	33.0	30.0	34.0
2	31.785679419243262	33.0	31.0	34.0	30.0	34.0
3	32.19232384972856	34.0	31.0	34.0	30.0	34.0
4	35.90669387645312	37.0	35.0	37.0	35.0	37.0
5	34.60268780369714	35.0	35.0	37.0	32.0	37.0
6	35.70441635561856	37.0	35.0	37.0	35.0	37.0
7	36.102666047471295	37.0	35.0	37.0	35.0	37.0
8	36.43872101872934	37.0	37.0	37.0	35.0	37.0
9	38.46464652857661	39.0	39.0	39.0	37.0	39.0
10	36.65398384184885	39.0	37.0	39.0	31.0	39.0
11	36.8139051555135	39.0	37.0	39.0	32.0	39.0
12	36.76111782697234	39.0	35.0	39.0	33.0	39.0
13	36.77955682172395	39.0	37.0	39.0	33.0	39.0
14	37.57051707033036	40.0	37.0	40.0	33.0	40.0
15	37.586531234820086	40.0	37.0	40.0	33.0	40.0
16	37.66282834100471	40.0	37.0	40.0	33.0	40.0
17	37.60931949690112	40.0	37.0	40.0	33.0	40.0
18	37.55055039295699	39.0	37.0	40.0	33.0	40.0
19	37.39418839783988	39.0	37.0	40.0	32.0	40.0
20	37.31183079827153	39.0	36.0	40.0	32.0	40.0
21	37.33775512141556	39.0	36.0	40.0	32.0	40.0
22	37.226255650685204	39.0	36.0	40.0	32.0	40.0
23	37.07557321721574	39.0	36.0	40.0	32.0	40.0
24	37.08702332109183	39.0	36.0	40.0	32.0	40.0
25	37.10797654441514	39.0	36.0	40.0	32.0	40.0
26	37.01474874328128	39.0	36.0	40.0	32.0	40.0
27	36.930509032393836	39.0	35.0	40.0	32.0	40.0
28	36.71430317577607	39.0	35.0	40.0	31.0	40.0
29	36.6504783205143	38.0	35.0	40.0	31.0	40.0
30	36.51945995509515	38.0	35.0	40.0	31.0	40.0
31	36.478888154011926	38.0	35.0	40.0	31.0	40.0
32	36.36560427719488	38.0	35.0	40.0	31.0	40.0
33	36.162806189211125	38.0	35.0	40.0	30.0	40.0
34	35.863853494366325	38.0	34.0	40.0	30.0	40.0
35	35.79075217997383	38.0	34.0	40.0	30.0	40.0
36	35.71608085721112	38.0	34.0	40.0	30.0	40.0
37	35.595567584331086	38.0	34.0	40.0	29.0	40.0
38	35.480713699143834	38.0	34.0	40.0	29.0	40.0
39	35.30616982830778	37.0	34.0	40.0	29.0	40.0
40	35.572070464855386	38.0	34.0	40.0	29.0	40.0
41	35.703355442933024	38.0	34.0	40.0	30.0	40.0
42	35.74706963416313	37.0	34.0	40.0	30.0	40.0
43	35.692874875593944	37.0	34.0	40.0	30.0	40.0
44	35.57467290503368	37.0	34.0	40.0	30.0	40.0
45	35.477454220945155	37.0	34.0	40.0	30.0	40.0
46	35.175299405224344	36.0	34.0	40.0	29.0	40.0
47	35.07835484958141	36.0	34.0	40.0	29.0	40.0
48	34.963745425259056	36.0	34.0	39.0	29.0	40.0
49	34.8236032898578	35.0	34.0	39.0	29.0	40.0
50	34.67578911808963	35.0	33.0	39.0	29.0	40.0
51	34.51018349596442	35.0	33.0	39.0	28.0	40.0
52	34.042700746673674	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	3.0
14	23.0
15	34.0
16	98.0
17	184.0
18	388.0
19	705.0
20	1437.0
21	2315.0
22	3800.0
23	5712.0
24	8481.0
25	11890.0
26	16388.0
27	22259.0
28	29601.0
29	38585.0
30	50553.0
31	67076.0
32	89690.0
33	127609.0
34	200066.0
35	240488.0
36	366534.0
37	518540.0
38	651585.0
39	73963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.52096746376205	0.34639867215820175	0.07654235818500861	0.0560915058947505
8	99.30910138084788	0.5002745240133354	0.10304539693640695	0.08757869820238195
9	95.65168203315491	2.6825426461583253	0.6089369828940685	1.0568383377927002
10	50.96949698023585	35.62487045156431	5.332174056135809	8.073458512064025
11	33.24022987232655	22.611245516239638	22.99890190394666	21.149622707487147
12	25.935003473084777	17.989827579932374	28.449667169301414	27.625501777681432
13	23.61946857847194	17.761070754410976	29.281031893835944	29.338428773281137
14	24.437581783630772	18.932386396900014	28.822885334405058	27.807146485064155
15	25.85327917747226	20.849505461208253	25.515701665973108	27.781513695346383
16	30.751910987764298	20.39187313984269	24.223302737487007	24.632913134906005
17	28.756904634946352	22.009824320454175	22.363817893269495	26.86945315132998
18	28.92632629908402	19.364227701450783	23.075839829874226	28.63360616959097
19	31.60950185363044	20.04017385993421	22.324182005465165	26.026142280970184
20	31.3678890764759	20.255797836402675	22.845421619834084	25.530891467287336
21	31.652816521440563	19.30069952199594	24.379077314506418	24.66740664205708
22	30.245307379870034	19.25975826064117	24.356213498986556	26.138720860502247
23	28.027042593152245	22.078099312819717	24.193437372923864	25.701420721104174
24	28.21932807280978	20.702275147428097	24.471323712070987	26.607073067691132
25	26.99852690572671	21.411409439512155	23.49541853440569	28.094645120355445
26	26.708259296237518	21.10654538032256	23.345379689653374	28.839815633786547
27	28.169249196601914	19.187488034075788	22.882525874086042	29.760736895236256
28	29.044917508302966	21.77810073686359	22.245345354373317	26.931636400460125
29	28.888233125475672	20.27134364868521	23.094550184097226	27.74587304174189
30	28.27961259677565	20.94606354716671	23.01326101300152	27.761062843056123
31	30.91678362286255	20.98747948981255	21.783440901388126	26.312295985936775
32	31.366109021634394	20.22747518603551	22.91357794187686	25.492837850453242
33	31.17323019036302	20.470551563837514	23.12129056349416	25.234927682305308
34	27.33547150883777	23.28568851730134	25.610756594509837	23.768083379351047
35	28.969284955925843	23.266147470818968	24.295019169212804	23.46954840404239
36	29.33993193070286	23.086520158923296	21.854880435694135	25.71866747467971
37	28.980716863685775	23.76278277160077	22.356658117129193	24.899842247584267
38	30.529087678381273	23.09633023893874	22.56235334325945	23.81222873942054
39	29.69792073771802	21.458640227973603	22.081817649599763	26.76162138470862
40	27.513120982020656	22.593998762664103	23.221606543006917	26.67127371230833
41	27.172853609872107	21.829999224687224	22.773902971979563	28.223244193461106
42	25.98496367896988	19.83254035186542	24.544938868961065	29.637557100203637
43	25.921316829192264	19.9602296191632	26.221157178051367	27.89729637359316
44	26.3291076149955	20.543850266533546	25.013488860021234	28.113553258449723
45	27.302916283625233	20.384476023056852	24.17144380643763	28.141163886880282
46	26.727919013042662	22.02651727919013	24.001310120363353	27.244253587403854
47	27.5603517704821	22.246808955020782	24.77365613770821	25.41918313678891
48	27.49480619554021	21.892894495753975	25.210204698395415	25.402094610310392
49	27.154578380165916	21.946493924870612	23.830622639449498	27.068305055513974
50	25.207237940326234	22.824614756575524	24.802809480334744	27.1653378227635
51	26.25031052067791	22.530391469660746	24.100281169551412	27.11901684010994
52	27.02265653802276	22.53074748062905	23.661754770151408	26.78484121119678
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	23.5
8	47.0
9	36.0
10	25.0
11	59.0
12	93.0
13	373.0
14	1253.5
15	1854.0
16	2664.0
17	3474.0
18	3013.0
19	2552.0
20	2524.0
21	2496.0
22	2548.5
23	2601.0
24	2942.0
25	3283.0
26	4426.0
27	5569.0
28	6438.5
29	7308.0
30	9065.5
31	10823.0
32	13048.5
33	15274.0
34	16615.0
35	17956.0
36	22457.0
37	26958.0
38	30111.5
39	39071.0
40	44877.0
41	46748.5
42	48620.0
43	52756.0
44	56892.0
45	60217.0
46	63542.0
47	68553.0
48	73564.0
49	86850.0
50	100136.0
51	104147.5
52	108159.0
53	116577.5
54	124996.0
55	135606.5
56	146217.0
57	154593.5
58	162970.0
59	185438.0
60	207906.0
61	218435.0
62	228964.0
63	226673.0
64	220644.0
65	216906.0
66	193880.0
67	170854.0
68	150977.0
69	131100.0
70	108685.5
71	86271.0
72	77553.5
73	68836.0
74	58752.0
75	48668.0
76	47541.5
77	46415.0
78	34140.0
79	21865.0
80	14563.5
81	7262.0
82	5203.0
83	3144.0
84	2002.0
85	860.0
86	544.5
87	229.0
88	155.5
89	66.5
90	51.0
91	30.5
92	10.0
93	6.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2528012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.01442183508786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.53129674566284	6.087555959359008
2	10.628266568063943	1.9161535644057985
3	4.119523199832882	1.114053596481736
4	1.8892339264511278	0.6812140623275927
5	1.0125153814417973	0.45636203814156256
6	0.6397109042566664	0.3459974366085056
7	0.43994013434615215	0.2776064167227008
8	0.35498592992470035	0.255999433428975
9	0.29137863943618075	0.23639489726505303
>10	7.42459900653456	19.117407230176493
>50	3.749679054906254	23.546639968309623
>100	1.7854566118803032	30.359389328937507
>500	0.09290538720264956	5.5849359421998415
>1k	0.03478448146449912	6.38110555512145
>5k	0.005283718703468221	3.145622122900609
>10k+	4.403098919556851E-4	0.4935624476135501
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	12435	0.4918884878711019	No Hit
CCAGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	9262	0.3663748431573901	No Hit
CCAGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	7966	0.31510926372184944	No Hit
CCAGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	7096	0.2806948701192874	No Hit
CCAGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	6892	0.2726252881711005	No Hit
CCAGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6750	0.2670082262267742	No Hit
CCAGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6467	0.25581365911237763	No Hit
CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6401	0.2532029120114936	No Hit
CCAGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	6127	0.24236435586539937	No Hit
CCAGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	5841	0.2310511184282353	No Hit
CCAGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5588	0.22104325454151325	No Hit
CCAGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5519	0.21831383711786176	No Hit
CCAGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	5343	0.21135184484883776	No Hit
CCAGACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	4912	0.19430287514458003	No Hit
CCAGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	4767	0.18856714287748635	No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4589	0.18152603705995066	No Hit
CCAGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	4508	0.17832193834522939	No Hit
CCAGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3809	0.1506717531404123	No Hit
CCAGACGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	3779	0.14948504991273776	No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	3688	0.14588538345545826	No Hit
CCAGACGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA	3602	0.1424835008694579	No Hit
CCAGACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3529	0.13959585634878316	No Hit
CCAGACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	3477	0.1375389040874806	No Hit
CCAGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3335	0.1319218421431544	No Hit
CCAGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3212	0.12705635890968872	No Hit
CCAGACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	3033	0.11997569631789723	No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3014	0.11922411760703668	No Hit
CCAGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2773	0.10969093501138445	No Hit
CCAGACGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	2755	0.10897891307477969	No Hit
CCAGACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	2666	0.10545836016601186	No Hit
CCAGACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2619	0.10359919177598842	No Hit
CCAGACGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT	2549	0.10083021757808111	No Hit
CCAGACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	2544	0.10063243370680203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0011075896791629153	0.0	0.0	0.0	0.0
8	0.0018196116157676466	0.0	0.0	0.0	0.0
9	0.002136065809814194	0.0	0.0	0.0	0.0
10	0.009889193563954601	0.0	0.0	0.0	0.0
11	0.01736542389830428	0.0	0.0	0.0	0.0
12	0.02499988132967723	0.0	0.0	0.0	0.0
13	0.03184320327593382	0.0	0.0	0.0	0.0
14	0.03896342264198113	0.0	0.0	0.0	0.0
15	0.04786369684954027	0.0	0.0	0.0	0.0
16	0.05379721298791303	0.0	0.0	0.0	0.0
17	0.058979150415425244	0.0	0.0	0.0	0.0
18	0.06546646139337946	0.0	0.0	0.0	0.0
19	0.07294269172772914	0.0	0.0	0.0	0.0
20	0.07859931044631117	0.0	0.0	0.0	0.0
21	0.08429548593914903	0.0	0.0	0.0	0.0
22	0.08971476401219615	0.0	0.0	0.0	0.0
23	0.09549005305354563	0.0	0.0	0.0	0.0
24	0.10142356919191839	0.0	0.0	0.0	0.0
25	0.1072384150075237	0.0	0.0	0.0	0.0
26	0.1136861692112221	0.0	0.0	0.0	0.0
27	0.12167663761089742	0.0	0.0	0.0	0.0
28	0.13488860021234075	0.0	0.0	0.0	0.0
29	0.1420879331268997	0.0	0.0	0.0	0.0
30	0.15245180798192415	0.0	0.0	0.0	0.0
31	0.15996759509052963	0.0	0.0	0.0	0.0
32	0.1672064847793444	0.0	0.0	0.0	0.0
33	0.17337734156325207	0.0	0.0	0.0	0.0
34	0.1833060919014625	0.0	0.0	0.0	0.0
35	0.19746741708504548	0.0	0.0	0.0	0.0
36	0.2071983835519768	0.0	0.0	0.0	0.0
37	0.21503062485462884	0.0	0.0	0.0	0.0
38	0.22412868293346708	0.0	0.0	0.0	0.0
39	0.2318818106876075	0.0	0.0	0.0	0.0
40	0.24010961973281772	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACG	45	3.110472E-10	46.000004	11
GCGTAAC	30	1.8622959E-6	46.0	31
CGGGATA	3195	0.0	43.912365	6
ACGGGAG	33570	0.0	43.75961	5
GACGGGA	91115	0.0	43.59183	4
ACGGGAT	25725	0.0	43.550243	5
ACGGGTA	2590	0.0	43.51351	5
CAGACGG	264645	0.0	43.461395	2
CCAGACG	266390	0.0	43.451256	1
AGACGGG	253445	0.0	43.410007	3
ACGGGAC	16010	0.0	43.18426	5
ACGGGGG	43980	0.0	43.102776	5
GACGGGG	131010	0.0	43.050606	4
ACGGGGA	34070	0.0	43.00939	5
ACGGGGC	32130	0.0	42.950516	5
ACGGGAA	16155	0.0	42.78242	5
AGACGGA	7295	0.0	42.75257	3
CGGGAGT	10140	0.0	42.733727	6
GACGGGC	20515	0.0	42.61418	4
ACGGGCT	10375	0.0	42.49735	5
>>END_MODULE