##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42469
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.574654453836917	31.0	30.0	33.0	28.0	33.0
2	27.920907014528243	30.0	27.0	31.0	16.0	33.0
3	30.921354399679768	31.0	30.0	33.0	28.0	33.0
4	35.49968212107655	35.0	35.0	37.0	35.0	37.0
5	32.675763498080954	35.0	32.0	35.0	28.0	37.0
6	34.833666909981396	35.0	35.0	35.0	33.0	37.0
7	35.949845769855656	37.0	35.0	37.0	35.0	37.0
8	36.43153829852363	37.0	37.0	37.0	35.0	37.0
9	38.24323624290659	39.0	39.0	39.0	37.0	39.0
10	35.963243777814405	38.0	35.0	39.0	31.0	39.0
11	35.747439308672206	37.0	35.0	39.0	31.0	39.0
12	36.24354234853658	37.0	35.0	39.0	33.0	39.0
13	36.2613671148367	38.0	35.0	39.0	32.0	39.0
14	36.84739457015706	39.0	35.0	40.0	32.0	40.0
15	36.99303021027102	39.0	35.0	40.0	32.0	40.0
16	37.194235795521436	39.0	35.0	40.0	33.0	40.0
17	37.154300784101345	39.0	35.0	40.0	33.0	40.0
18	37.050625161882785	39.0	35.0	40.0	33.0	40.0
19	37.02241635074996	38.0	35.0	40.0	33.0	40.0
20	36.9354823518331	38.0	35.0	40.0	33.0	40.0
21	36.768772516423745	38.0	35.0	40.0	32.0	40.0
22	36.52862087640396	38.0	35.0	40.0	32.0	40.0
23	36.15300572182062	38.0	35.0	40.0	31.0	40.0
24	35.99616190633168	37.0	34.0	39.0	31.0	40.0
25	35.82650874755704	37.0	34.0	39.0	31.0	40.0
26	35.28401893145588	37.0	34.0	39.0	30.0	40.0
27	35.248675504485625	37.0	34.0	39.0	30.0	40.0
28	35.118462878805715	36.0	34.0	38.0	30.0	40.0
29	34.816807553745086	36.0	34.0	38.0	29.0	40.0
30	34.501212649226495	36.0	33.0	38.0	29.0	40.0
31	34.08975958934752	35.0	33.0	38.0	27.0	39.0
32	34.0797758364925	35.0	33.0	38.0	27.0	39.0
33	33.531046174856954	35.0	32.0	38.0	25.0	39.0
34	32.89166215357084	35.0	31.0	38.0	23.0	39.0
35	32.395700393228005	34.0	31.0	38.0	22.0	39.0
36	32.401116108220116	34.0	31.0	37.0	23.0	39.0
37	31.87407285313994	34.0	30.0	37.0	22.0	38.0
38	31.499187642751185	34.0	30.0	36.0	21.0	38.0
39	30.696555134333277	33.0	29.0	36.0	18.0	38.0
40	30.571993689514706	33.0	29.0	36.0	18.0	38.0
41	29.964209187878218	32.0	28.0	35.0	18.0	38.0
42	29.659610539452306	32.0	27.0	35.0	18.0	37.0
43	29.103934634674705	31.0	27.0	34.0	17.0	37.0
44	28.70872871977207	31.0	26.0	34.0	17.0	37.0
45	28.13901904918882	31.0	24.0	34.0	17.0	36.0
46	28.160634815983425	31.0	24.0	34.0	17.0	36.0
47	27.987590948691988	30.0	24.0	33.0	16.0	36.0
48	27.58127104476206	30.0	23.0	33.0	15.0	35.0
49	27.11121053003367	29.0	24.0	33.0	13.0	35.0
50	30.09607007464268	33.0	28.0	35.0	18.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	4.0
12	2.0
13	4.0
14	8.0
15	4.0
16	16.0
17	20.0
18	38.0
19	50.0
20	78.0
21	136.0
22	195.0
23	269.0
24	375.0
25	504.0
26	743.0
27	1003.0
28	1232.0
29	1366.0
30	1751.0
31	2601.0
32	3731.0
33	3965.0
34	5582.0
35	9674.0
36	8172.0
37	917.0
38	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.02915067460971	1.3138995502601898	0.5745367209023052	0.08241305422778968
8	96.39737220089948	2.952742000047093	0.3696814146789423	0.2802043843744849
9	94.67376203819256	2.9951258565070993	1.7377381148602509	0.5933739904400857
10	46.16072900233112	38.781228660905604	5.048388236125174	10.009654100638112
11	33.24542607549036	29.887682780380985	18.22270361911041	18.64418752501825
12	27.12566813440392	27.00087122371612	25.938920153523746	19.934540488356213
13	27.39174456662507	23.108620405472227	22.588240834491042	26.911394193411663
14	22.060797287433186	23.4712378440745	31.573618404012336	22.894346464479973
15	22.840189314558852	27.792036544302906	30.48341142951329	18.88436271162495
16	28.448986319433	27.029127128022797	27.606018507617318	16.91586804492689
17	30.31387600367327	30.68826673573665	17.723515976359227	21.27434128423085
18	27.41293649485507	29.6286703242365	23.09213779462667	19.866255386281757
19	29.080034848948642	30.273846805905485	22.227978054580987	18.41814029056488
20	24.500223692575762	33.75167769431821	22.049023993972074	19.699074619133956
21	28.47724222373967	27.935670724528478	22.67300854741105	20.9140785043208
22	29.228378346558664	31.87736937530905	22.713037745178834	16.181214532953447
23	27.33758741670395	30.33742259059549	24.07873978666792	18.246250206032634
24	28.691516164731922	25.39970331300478	24.59911935764911	21.309661164614187
25	26.056653088134873	30.229108290753253	22.510537097647696	21.203701523464176
26	23.774988815371213	29.868845510843204	20.47375732887518	25.882408344910406
27	22.755421601638844	28.44427700204855	26.026042525135985	22.774258871176624
28	23.95394287598013	29.282535496479785	22.567048906261036	24.19647272127905
29	27.96392662883515	30.68355741835221	19.765005062516188	21.587510890296453
30	29.117709388024203	26.791306600108317	20.63151946125409	23.459464550613387
31	27.881513574607357	26.51581153311827	24.98999270055805	20.61268219171631
32	29.95832254114766	27.196307895170595	24.302432362429066	18.54293720125268
33	31.63719418870235	29.50151875485648	20.96823565424192	17.89305140219925
34	27.872094939838473	27.4576750100073	25.12185358732252	19.548376462831712
35	23.52774965268784	27.30933151239728	28.208811132826295	20.95410770208858
36	23.360568885540044	36.30412771668747	21.472132614377546	18.86317078339495
37	28.00160116791071	28.924627375261956	21.839459370364267	21.234312086463067
38	26.913748852103886	31.7572817820057	19.063316772233865	22.265652593656547
39	25.152464150321414	34.46513927806165	20.520850502719632	19.86154606889731
40	30.207916362523253	25.62339588876592	19.01386893969719	25.154818809013634
41	22.2185594198121	28.079304904754053	25.503308295462574	24.198827379971274
42	21.957192304975393	29.353175257246463	22.61649673879771	26.073135698980433
43	22.067861263509855	25.38322070215922	24.024582636746803	28.52433539758412
44	25.373802067390333	25.53862817584591	28.048694341755166	21.038875415008597
45	25.33377286962255	24.893451694176928	24.653276507570226	25.119498928630296
46	26.49932892227272	29.303727424709784	23.216934705314465	20.98000894770303
47	24.210600673432385	30.824836939885564	23.132166992394453	21.832395394287598
48	24.43429324919353	24.182344769125717	24.118768984435707	27.264592997245053
49	22.536438343262144	25.931856177447077	25.741128823377053	25.79057665591373
50	25.63987849961148	24.542607549035765	23.659610539452306	26.157903411900445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	1.0
10	0.0
11	5.0
12	10.0
13	49.0
14	88.0
15	118.5
16	149.0
17	132.0
18	115.0
19	127.5
20	140.0
21	115.5
22	91.0
23	142.0
24	193.0
25	237.5
26	282.0
27	323.0
28	364.0
29	622.5
30	881.0
31	757.5
32	634.0
33	668.0
34	702.0
35	786.5
36	871.0
37	787.0
38	703.0
39	1048.0
40	1393.0
41	1217.5
42	1042.0
43	1444.5
44	1847.0
45	1895.0
46	1943.0
47	1660.5
48	1378.0
49	1680.0
50	1982.0
51	2399.5
52	2817.0
53	2610.0
54	2403.0
55	3705.0
56	5007.0
57	3511.0
58	2015.0
59	2370.0
60	2725.0
61	2681.0
62	2637.0
63	3242.0
64	3847.0
65	3488.0
66	3129.0
67	2185.5
68	1242.0
69	858.0
70	474.0
71	383.0
72	292.0
73	399.0
74	506.0
75	474.0
76	442.0
77	254.0
78	66.0
79	59.0
80	52.0
81	28.0
82	4.0
83	2.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	42469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.103345970001648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93344015777147	15.269961619063317
2	6.951805743867865	2.6560550048270506
3	2.810304449648712	1.6105865454802324
4	1.1956119807716012	0.9136075725823543
5	0.7149020091211635	0.6828510207445431
6	0.3451251078515962	0.3955826602933905
7	0.4437322815234808	0.5933739904400857
8	0.24651793417971157	0.37674539075561
9	0.2958215210156539	0.5086062775200735
>10	4.893380993467275	24.29772304504462
>50	1.2695673610255147	16.45906425863571
>100	0.8751386663379761	33.10885587134145
>500	0.02465179341797116	3.1269867432715626
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	772	1.817796510395818	No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	556	1.3091902328757448	No Hit
GAGGAGGGGCAACCATGGCAGGCACTAGAGGGAGAGTAAGGCTATAGAAG	471	1.1090442440368269	No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	448	1.054887094115708	No Hit
GAGGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	438	1.0313405071934825	No Hit
GAGGAGGGGGGCCCAGCGGGGAAGTGGACCTCCGTTCCAGGACGCCTGGG	382	0.8994796204290189	No Hit
GAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	340	0.8005839553556712	No Hit
GAGGAGGGGAATTAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGG	339	0.7982292966634487	No Hit
GAGGAGGGGAGTCCAGAAGGTAGTAGAGGGAGAGGAAGGGAGAGAAGAGA	333	0.7841013445101133	No Hit
GAGGAGGGGGGGACGTGTTTCAACAGATGGTTGGGGTTAGTGTGTGTCAT	314	0.7393628293578846	No Hit
GAGGAGGGGAGAAGCTGGCCTGTGCCTCTAATCCCAGCACTTGGGAAGCT	302	0.7111069250512139	No Hit
GAGGAGGGGGGAGCTGGCCATGGCGGCCGCGGTGTCCGGTGTGGTGAGGC	284	0.6687230685912077	No Hit
GAGGAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	271	0.6381125055923145	No Hit
GAGGAGGGGGGGGTTAGAGAGGTGGCTCAGCGGTTAAGAGTACTGACTGC	259	0.6098566012856436	No Hit
GAGGAGGAGCTCTTGTCAAAAGAGAAGCAAGAAAAGGAAAAACAAGAACA	258	0.607501942593421	No Hit
GAGGAGGGGTCCATGAAAGAAAATTCAAACTACAGTGGGCTTTAAGGATG	254	0.5980833078245308	No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	245	0.5768913795945279	No Hit
GAGGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	243	0.5721820622100826	No Hit
GAGGAGGGGGAACTTTCGGATGCTACTTTCGGGAATCACCACTTGTATGC	242	0.5698274035178601	No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	242	0.5698274035178601	No Hit
GAGGAGGGGATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCAC	241	0.5674727448256376	No Hit
GAGGAGGGGGTTGTAGTTTGAACAGATGACCTACCACAAGCCTCACTCCT	237	0.5580541100567472	No Hit
GAGGAGGGGGAAAGAGAGGCCCATTGGTCGTGCAAACTTTATATGTCTCA	233	0.5486354752878569	No Hit
GAGGAGGGTGGGGCTGACAAGATGGCTCAGTGGGTAAGAACACTCACTGT	229	0.5392168405189668	No Hit
GAGGAGGGGAGGGTTGGTTCTTTGGTTTTGTTTTTTTGTTTGTTTGTTTG	228	0.5368621818267442	No Hit
GAGGAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	223	0.5250888883656314	No Hit
GAGGAGGGGGCTTTCCGGTCGCGAGCTGTAGATACCACCTCGCGTCGCAA	221	0.5203795709811863	No Hit
GAGGAGGGGAAACTCCCATAGCCTGCTGCAGCTGTGGGTGATACTTGAAG	216	0.5086062775200735	No Hit
GAGGAGGAGAGATGACCTCTCTGTCCTTGGGCTGCATCTCTCCTCATCCT	202	0.4756410558289576	No Hit
GAGGAGGGGGGGGGTTAGAGAGGTGGCTCAGCGGTTAAGAGTACTGACTG	199	0.4685770797522899	No Hit
GAGGAGGGGACAGTACTACTATAACTCCTTAACACAGCAGTACTTGTACT	199	0.4685770797522899	No Hit
GAGGAGGGGAAGCAGGCAGAGTGCAGTGCTGAGAGGGGACAAGGATATTT	199	0.4685770797522899	No Hit
GAGGAGGGGAAATGACAGTTCAAATGGAGAGATTCAACACTATGACAAGG	198	0.46622242106006734	No Hit
GAGGAGGGGAGCCTCAGGGGTTTCTTTCTGAACCACTACTTCTCCAAGTA	192	0.45209446890673194	No Hit
GAGGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGATTTCT	189	0.44503049283006424	No Hit
GAGGAGGGGATTCTCTTTACCAATATCTTTTATTTTTTCATTTTTGATTG	181	0.42619322329228376	No Hit
GAGGAGGGGATAGAGAAAGAGTGTTGCTGGGGAGAGCTACCTCTCCCTTG	178	0.41912924721561606	No Hit
GAGGAGGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG	174	0.4097106124467258	No Hit
GAGGAGGGGGCACCCCAAGGAAGAACACAAATTTCAAAGAAAAGGAAATG	174	0.4097106124467258	No Hit
GAGGAGGGAAGAACCACAGACAACTAAGGAATGCTGAAAGCAGAAGAAAT	170	0.4002919776778356	No Hit
GAGGAGGGGATCTTCCGCAAGAGCAAGCATTGCTGGCGTGACACCGACCT	168	0.3955826602933905	No Hit
GAGGAGGGGCCCTTGTCTTCCAGGGCATCAAGACAGTGTGTGATCCTTCC	168	0.3955826602933905	No Hit
GAGGAGGGGGTGCTTTATGCAATCTAAAGCTTTTTCTATCTCTGTGTGCC	166	0.3908733429089453	No Hit
GAGGAGGGGGAAGGGTTACATGTTAATGGACCAACAGGTGGAAATAAGAA	164	0.38616402552450024	No Hit
GAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAG	163	0.3838093668322777	No Hit
GAGGAGGGGGGATAAATAAAAATAAAGTCACATTTAGATACACTGTAGGA	161	0.37910004944783254	No Hit
GAGGAGGGGACATGCAGAAGAAAGAGCCATCTACCCAACCTCCCAAGCAC	156	0.3673267559867197	No Hit
GAGGAGGGGCATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTA	149	0.3508441451411618	No Hit
GAGGAGGGGGACGGACATTGGGAGTGTATGCTAATATTTCTCCTATATAA	148	0.3484894864489392	No Hit
GAGGAGAGGAGAGGAGAGGAGAGGAGAGAAGAGAAGAGAAGAGAAGAGAA	146	0.3437801690644941	No Hit
GAGGAGGGGGCTCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCC	139	0.3272975582189362	No Hit
GAGGAGGGGATTACAAGATGGCCAATACCGGGGATAAAAACCTTTTAGAC	138	0.3249428995267136	No Hit
GAGGAGGGGCTTGAGAAGATGCTCCCTCTAGCTTGTTAGGATGCTGAGGG	134	0.31552426475782336	No Hit
GAGGAGGGGAATCCCAGGACTTGGGAGGCAGAAGCAGGTGGATTTCTGAG	133	0.3131696060656008	No Hit
GAGGAGGGGAACATGCCCGGAGACGCGCCCATTGCCCCTGTGGAAGGGAC	132	0.3108149473733782	No Hit
GAGGAGGGGGCCTTTCCCGTCCTGGGCTGAACGAGGCAGGCTCGTGGACC	129	0.3037509712967105	No Hit
GAGGAGGGGGCTGTTAGGAATGAAGGAAAACCTTACCCAGGCAACTTCCT	126	0.2966869952200428	No Hit
GAGGAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	124	0.2919776778355977	No Hit
GAGGAGGGGAACTCCAATGTCCACAACACTCAGGAGAGTAAAACCTGAGT	123	0.2896230191433752	No Hit
GAGGAGGGGGCGCGCCAGGCCATCGGCCATATGCACCGCCGCCTGCTCAT	122	0.2872683604511526	No Hit
GAGGAGGGGGGAGCCTGAGCCTGCTGGCACGCGCTTGCAAACCCGGCATT	122	0.2872683604511526	No Hit
GAGGAGGGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTC	115	0.2707857496055947	No Hit
GAGGAGGGGACTGCATTGGGTCAGACGCCCACTAGAGACTATCTTGGGAA	115	0.2707857496055947	No Hit
GAGGAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	115	0.2707857496055947	No Hit
GAGGAGTGGCATTAACACCATCCTTCATGAACTTAATCCACTGTTCACCA	111	0.2613671148367044	No Hit
GAGGAGGGGACCAGTCCCTTTCAGCAGTATCTGTCCTCACCTTTTCCTCC	111	0.2613671148367044	No Hit
GAGGAGGGGCAGGTGCGCTTCGTGCTGTCCTTATTGTTCATCCTTTTCCC	108	0.25430313876003674	No Hit
GAGGAGGGGAGGGTTTCAGAAGAAAGAGAGAGATCAGATAGGCCCAGGAA	106	0.24959382137559163	No Hit
GAGGAGGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTG	105	0.24723916268336904	No Hit
GAGGAGGGGCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC	105	0.24723916268336904	No Hit
GAGGAGGGGGAGCGGCCCACCATCTACATCCGTAGAGCGGGTGGACTTTC	105	0.24723916268336904	No Hit
GAGGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGT	104	0.2448845039911465	No Hit
GAGGAGGGGGGACCTCATTCATTTCCATCGGTCCTTGCCCTCGCAGCTCC	102	0.24017518660670134	No Hit
GAGGAGGGGACTTGGACAACAACAAACTCCTCAGGGTGCCTGCTGGGCTG	100	0.2354658692222562	No Hit
GAGGAGGGGGTGCAGTGCTGAGAGGGGGCTCTCGGTCTTAGCGCCATCTT	100	0.2354658692222562	No Hit
GAGGAGGGGGGCTTTTGAAGACGCCCCCCGACACAAAAATTTTCATCTCG	99	0.23311121053003367	No Hit
GAGGAGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCT	96	0.22604723445336597	No Hit
GAGGAGGAGGTGGCTTAGAGCTGTCTTAGTCACTAAAGCATGGGAGCAGT	96	0.22604723445336597	No Hit
GAGGAGGGGAAGTGTTGGTAGTGTTTGTCCGGGTGCCTCAGTTGTAACTT	95	0.22369257576114343	No Hit
GAGGAGGGGGACAGTGTTTACAAACTTTGCAAGGTCCCAGCAAGCATCAG	94	0.22133791706892086	No Hit
GAGGAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCA	94	0.22133791706892086	No Hit
GAGGAGGGGGAGAATTCTGGGTGGAAATGTCTGATCAGGAGAAGGGCCTG	94	0.22133791706892086	No Hit
GAGGAGGGGCATCAACCTCACAAGTCTCCTCTTGCTTTGGCACAGATGTA	92	0.21662859968447573	No Hit
GAGGAGGGGAAATGAAGGGTGACTACTACCGTTACTTGGCCGAGGTTGCT	91	0.21427394099225316	No Hit
GAGGAGGGGGCTCTGGATTGGATCTCCTGACATCATGAGGTTCCGGTTCT	90	0.21191928230003063	No Hit
GAGGAGGGGGCACTTAGTTCCGCCAACCCTAGATGTGCATCGGGCCTTCC	89	0.20956462360780803	No Hit
GAGGAGGGGAGAGTATTACCATATTTTACAAATGATTTTCATTAGAAATT	88	0.2072099649155855	No Hit
GAGGAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA	86	0.20250064753114036	No Hit
GAGGAGGGGGCTTTCGTTCCGGCGGCTCCTAGAGCGTTTGCTTTGTTCCC	85	0.2001459888389178	No Hit
GAGGAGGGGAATAATTCTGCTCAATGTAGAAATGGTCAGTCGATATACAC	84	0.19779133014669525	No Hit
GAGGAGGGGGTTTGCAGCTACAGGAGGTAGCGTGGCATGCCTCTCGTTCT	83	0.19543667145447266	No Hit
GAGGAGGGGGCTCTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCTTC	83	0.19543667145447266	No Hit
GAGGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT	83	0.19543667145447266	No Hit
GAGGAGGGGAACATGGTTGGTGGCTTTGGCTTACCTACTGGCTTAAATGA	83	0.19543667145447266	No Hit
GAGGAGGGGGGTTGGAGTCACAGTCTTCTTCAGCATCCTGGTATTTGGGA	82	0.19308201276225012	No Hit
GAGGAGGGGAACCATGATCATAGACAACTGCTGCATGCAGCTGGTGCAGA	81	0.19072735407002755	No Hit
GAGGAGGGGGCTCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCA	81	0.19072735407002755	No Hit
GAGGAGGGGATCAAGGCAGGAGCATGGCAGCATCCAGGCAGGCATGGTGC	80	0.188372695377805	No Hit
GAGGAGGGGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTT	79	0.18601803668558242	No Hit
GAGGAGGGGATGCTTTTGTGTGCCCATCACCCACCATACCATATTCACCT	79	0.18601803668558242	No Hit
GAGGAGGGGATGGTGAACGTGCCTAAAACCCGCCGGACATTCTGCAAGAA	78	0.18366337799335986	No Hit
GAGGAGGGGACTCATGGAAAGTTGGCCCAAACCCAACCTGACCCCACACT	78	0.18366337799335986	No Hit
GAGGAGGGGATGTGTATGTTCCTTTTTGATTGTTTGCATGTTATGATTAA	78	0.18366337799335986	No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76	0.17895406060891475	No Hit
GAGGAGGGGATCTTGTATATTGTGAATAGTACCATAATAAACATGAATGT	76	0.17895406060891475	No Hit
GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAA	73	0.17189008453224705	No Hit
GAGGAGGGGGGGGCGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCG	73	0.17189008453224705	No Hit
GAGGAGGGGCACATGGTGGCTCACAACCACCACCAACTACAGTTCCAGGG	72	0.16953542584002448	No Hit
GAGGAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGTGT	72	0.16953542584002448	No Hit
GAGGAGGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAAAGCACCAACTG	71	0.16718076714780192	No Hit
GAGGAGGGTGGAGGAGGAGGCTATGATGGTTACAATGAAGGAGGAAATTT	67	0.15776213237891168	No Hit
GAGGAGGGGGATTCCACAGCTTTGCTCATTCACTCTCTCACTTGGGACTA	67	0.15776213237891168	No Hit
GAGGAGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGCCTGCTC	67	0.15776213237891168	No Hit
GAGGAGGGGATACCAAGAACTTTCCCCATGGAGCCATCTCCCAGTTCTGC	66	0.1554074736866891	No Hit
GAGGAGGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAG	66	0.1554074736866891	No Hit
GAGGAGGGGCAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCC	66	0.1554074736866891	No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	66	0.1554074736866891	No Hit
GAGGAGGGGAGGGAAGCAAGTGGACGTGGGTTGGATGAGAGAGCTAGGTG	65	0.15305281499446655	No Hit
GAGGAGGGGGTTTTGTACTTTGATTATTTTTATTTCTTACACTTTTCTTT	65	0.15305281499446655	No Hit
GAGGAGGGGAGAATCACTTTTAAAGTTGTCTGTAGTTGTGAACTGAGTTA	64	0.150698156302244	No Hit
GAGGAGGGGGAGGATGGCCTAATCGGTCATCAATGGAAGGAGAGGCCCTT	63	0.1483434976100214	No Hit
GAGGAGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATA	63	0.1483434976100214	No Hit
GAGGAGGAGGTACTAACAAGGAGACCGGGGTGAGCCCTGCCCAGGTGTCT	63	0.1483434976100214	No Hit
GAGGAGGGGAGTGTGTGAATCCCCTCTGCCTTAGGAGACCTTCTCATCAG	63	0.1483434976100214	No Hit
GAGGAGGGGGGTCTCCCCTCAGGACACTGTACAGCGATGCCGTGAGCTCT	63	0.1483434976100214	No Hit
GAGGAGGGGGACTACTCTGGTTGCGCTTGTTGGATCCCAAACCGGCTCCC	63	0.1483434976100214	No Hit
GAGGAGGGGATGTCACTGTCGGCGTGGGGTCTGCCTGACTGCTCTAGAGC	62	0.14598883891779885	No Hit
GAGGAGGGGGCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTC	62	0.14598883891779885	No Hit
GAGGAGGGGTGCCGGCAAGCAGCTGGAAGATGGCCGCACCCTCTCTGATT	62	0.14598883891779885	No Hit
GAGGAGGGGAGGTGTTGGTGGCGCACGCCTTTGATCCCGGCACTCGGGAG	61	0.1436341802255763	No Hit
GAGGAGGGGATTCCCAGCCATTTGCAGGAAGTGGCTGTGGGCCCGTCGGG	61	0.1436341802255763	No Hit
GAGGAGGGGAGCTGTTACCATGCCAACACCACGTGCCCTGGTTGGTTCCA	61	0.1436341802255763	No Hit
GAGGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	60	0.14127952153335374	No Hit
GAGGAGGGGAGCTTTCATTTCTGTTTTGCATTTGACTCTTAACACTCACC	60	0.14127952153335374	No Hit
GAGGAGGGGATAATCTTACGGAGGGTTGGGGCTTGGTGGATACTATGTAA	60	0.14127952153335374	No Hit
GAGGAGGGGGAGGGGGAACTCTTCTGCCAAGAGGACAGTGCTAAGAAGCA	59	0.13892486284113117	No Hit
GAGGAGGGGAGTGCCCTTTCCTGGGGCCCCCACGCAAACGCCTGAAGCAA	59	0.13892486284113117	No Hit
GAGGAGGGGAATTACGCCGGAGCAAAGTTCAGTGACCCACCTTCCCCTAG	59	0.13892486284113117	No Hit
GAGGAGGGGGTTGCTGACACTATGGAGTGTCCAGATTCACTTTCCTAGGA	59	0.13892486284113117	No Hit
GAGGAGGGGGGGAATTCTTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	59	0.13892486284113117	No Hit
GAGGAGGGAGGCACCGAACTGGAGGAGAAAAAAAAGGAAAAAGAAAGGAA	59	0.13892486284113117	No Hit
GAGGAGGGGGACAGATGCTAACAAATCACAGGAGGAACAGGCTCCAGACA	59	0.13892486284113117	No Hit
GAGGAGGGGTGAAGCCCCACCCCTGCCTCTCCACCCCTCCACCAGGCCTT	59	0.13892486284113117	No Hit
GAGGAGGGGACCACGCTTGGCTTATGGCTATACTTCTTAAGCCCTTTTTA	58	0.1365702041489086	No Hit
GAGGAGGGGATTTCATGCTCATTTTCTTACTCTTGGCTGTCTCTAAATTG	58	0.1365702041489086	No Hit
GAGGAGGGGGCTTTCCTTTCAGCGGCGCGCGGCTGCAAGATGGCGGTGCA	58	0.1365702041489086	No Hit
GAGGAGGGGCAGAAGATGAGGATGAAGATGTTCAAATGGAGAGAATGAGA	58	0.1365702041489086	No Hit
GAGGAGGGGGCAGCTGGCATTCCTCAGACTTCTTTCCAGCCGGGCCTCCC	58	0.1365702041489086	No Hit
GAGGAGGGGGGACGGCGGACGCTGTAGCTGCTCTGAAGTGTGCCAGCCTA	57	0.13421554545668604	No Hit
GAGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56	0.1318608867644635	No Hit
GAGGAGGGGAGGAAGGGGGTCTTAGTGTGTCTGCAACCTCAGAGGCACTG	56	0.1318608867644635	No Hit
GAGGAGGGGATCTTCCGCAAGGGCAAGCATTGCTGGCGTGACACCGACCT	56	0.1318608867644635	No Hit
GAGGAGGGGTCTCCATGTTTTCTTTTTTAACTTTGCACTTTCTTTGTATA	56	0.1318608867644635	No Hit
GAGGAGGGACTTCTACAGAGACCCAGAGGAGATTGAGAAGGAGGAGCAGG	55	0.12950622807224094	No Hit
GAGGAGGGGATCTCTGTACTCGGGGGACTGACTCAGATGGACCTCTGTGA	55	0.12950622807224094	No Hit
GAGGAGGGGACCCGGAGAGAAGCCAGAATCACCTTGGGCTGCACCTAAGC	55	0.12950622807224094	No Hit
GAGGAGGGGCACGTGTAGCTCGGACCTTCGAGGGCATCAGAGGTCTCACG	55	0.12950622807224094	No Hit
GAGGAGGGGGCTCTTTCTGCCATCTTCCCTCGCCGCCACCATGGTGCGAA	55	0.12950622807224094	No Hit
GAGGAGGGGTCTATTTTGACTTTCAAAACCTTTTACTTCCCAGCCCGAAC	54	0.12715156938001837	No Hit
GAGGAGGGGGTACTGAGTATATTGATCATCTGAAGATGCCAAACCAAGAG	54	0.12715156938001837	No Hit
GAGGAGGGGAACTCTGAAACACATTTTTTGTCTTGAAGTAATATGAAAGC	54	0.12715156938001837	No Hit
GAGGAGGGGGGGCCGGACTGGCGTTGCCCCGGCTGCCCGCGCACTACCAG	54	0.12715156938001837	No Hit
GAGGAGGGGCTTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAA	54	0.12715156938001837	No Hit
GAGGAGGGGGGTGGCTGGGGGCTCTTAAGGATTTCCCAGAAAAACAGGAT	54	0.12715156938001837	No Hit
GAGGAGGGGTGATCACAACCATAAAGGAAATTCTGGTATAAAAACAAATT	53	0.12479691068779582	No Hit
GAGGAGGGGGTTGGGAGGCCACTATGCTGGTGTGGATGAGAGATGATGGT	53	0.12479691068779582	No Hit
GAGGAGGGGGAGCAGGTGTCTGTGGGTGTGGTGGTTTATTATGATCAATA	53	0.12479691068779582	No Hit
GAGGAGGGGAAGGTGGTGGACTTGAGGCTGAAGAAGCTATTAGAAGCCCA	53	0.12479691068779582	No Hit
GAGGAGGGGAGGAAAATTTAAAGATGACAGGCAGGGGAGAAGATGCATAT	53	0.12479691068779582	No Hit
GAGGAGGGGGCAGTTTCCCGAGACCGGCAGGTCACTTGTTTGTGTGCCGC	53	0.12479691068779582	No Hit
GAGGAGGGGGAACAGTAAGCCTTCTAAGGGCTGAGGTGTGCCTTCCGTGC	52	0.12244225199557325	No Hit
GAGGAGGGGCTCCAGGAACTTCGTGTCCATGAGAATGAGATCACCAAGCT	52	0.12244225199557325	No Hit
GAGGAGGGGGTAACAGCAATGCGTTTGACCTAGCTAATAAAACATGTGTG	52	0.12244225199557325	No Hit
GAGGAGGGGATTTGATTCCTGAACGTTTGGGCTGGACCCTGCACATCTTC	52	0.12244225199557325	No Hit
GAGGAGGGGGTTTTTTCTCAACTGCTTCACTATTTTAAACAATTTCTGCT	51	0.12008759330335067	No Hit
GAGGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTG	51	0.12008759330335067	No Hit
GAGGAGGGGCCCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGTG	50	0.1177329346111281	No Hit
GAGGAGGGGGACTATGAAAACTTACTATAATTTGTAAAGCCATATTCAAA	50	0.1177329346111281	No Hit
GAGGAGGGGGACCCTGGGGGTGTATTATATATAGTGTATATTTTTTTCAA	50	0.1177329346111281	No Hit
GAGGAGGGGAAGGCAAGCTTGTAACACAGTGAATGAAGCAGAGTCCTTAA	50	0.1177329346111281	No Hit
GAGGAGGGGATTTGCAAAGACCCCAAAGACCCCCTGTTCCCTCTTGCCCC	50	0.1177329346111281	No Hit
GAGGAGGGGGGGCACGTCTGCCGACAGCGGGATTGACACCGCCTCCTACG	50	0.1177329346111281	No Hit
GAGGAGGGGGTTTCCATTTGCTTCCAAGCTGTGCCCGGTCTGTTCAGAGG	49	0.11537827591890555	No Hit
GAGGAGGGGATCACACTCTTGAAAAACAACAAGAACCAAGAAAATAATGT	49	0.11537827591890555	No Hit
GAGGAGGGAGTCTCAGGGCCAGGTAATCGTGAGCATCCCCACAGGTTCAT	49	0.11537827591890555	No Hit
GAGGAGGGGGGTCTTCCGGTCGGGCCGAGCTCGTCGCTAGGTTTGTTGAA	49	0.11537827591890555	No Hit
GAGGAGGGGGCCTACTTCTCTCACTGAAGTGCATCTAGATGGCAACAAGA	48	0.11302361722668298	No Hit
GAGGAGGGGGTGAAATTTTCTGTACGTTCCATGTGCAGAGAAAAGCCTCC	48	0.11302361722668298	No Hit
GAGGAGGGGATGGGAAAAGACTTGATCTGTGGCATAAAGCCTTGCTCATG	48	0.11302361722668298	No Hit
GAGGAGGGGCATCTAGATATGGCCTTGCCCTTATGCCAGCTTTTCATGTT	48	0.11302361722668298	No Hit
GAGGAGGGGTGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTC	47	0.11066895853446043	No Hit
GAGGAGGAGGGTGCTCACTCAAAATGATTGCATTGTGAAATTAAGATGAC	47	0.11066895853446043	No Hit
GAGGAGGGGAAGTAAGATGACATTTCTGACAGGCGGGTGCCAGTCTGCAT	46	0.10831429984223787	No Hit
GAGGAGGGGACCCTAACATGAGGGCAGGACACACACACACACACACACAC	46	0.10831429984223787	No Hit
GAGGAGGGGAAATGGAAGCTTGACTTTGCCAAGACTTGAATTCCTTCGGG	46	0.10831429984223787	No Hit
GAGGAGGCTGGGTAGGGTCCTATCAACGCTCCTTTTCTATCCCTTGGAGC	46	0.10831429984223787	No Hit
GAGGAGGGGATAGTGTGACTCATCCAAAACAAACAAACAAACAAACAAAA	46	0.10831429984223787	No Hit
GAGGAGGGGCCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGA	46	0.10831429984223787	No Hit
GAGGAGGGGAAGATGAGGCGGGGCGAGGAGCTGCTCCAGTCCCTCTCCAA	45	0.10595964115001531	No Hit
GAGGAGGGGGGAGTCATTTCTCCCTGGAGATGCACAAGGGCGTGCAGCAC	45	0.10595964115001531	No Hit
GAGGAGGGGGTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATC	45	0.10595964115001531	No Hit
GAGGAGGGGGACTTGGAGGCTCCCGAAGGAGGCTGCCTGTCGCCCAAGCA	45	0.10595964115001531	No Hit
GAGGAGGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCG	44	0.10360498245779275	No Hit
GAGGAGGGGAGGTCGTCTACCTTCACAACAACAACATCTCCGCAGTTGGG	44	0.10360498245779275	No Hit
GAGGAGGGGAGAGATGTGTGGAGACAGAGAGCAGTAATCCATGTACTTTC	44	0.10360498245779275	No Hit
GAGGAGGGGGCTCCTGAGCTACGTGTCTCTACGCTCTCAATCCGCCATCC	44	0.10360498245779275	No Hit
GAGGAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT	44	0.10360498245779275	No Hit
GAGGAGGGTTATCTTGTGTTCCTAGTACCACACCAATCCCCTCCCTGACC	44	0.10360498245779275	No Hit
GAGGAGGGGAGACTTTGAAAGGCAGTAGTCCAGTCACCTCTCTTGATGGG	44	0.10360498245779275	No Hit
GAGGAGGGGAGTAACATTAAGAGTAACTATCTATCAAAATAAATATTGAA	44	0.10360498245779275	No Hit
GAGGAGGGGATCCCTAAACCATTTTTTAAAGTTTTTTGTTTTGTTATGCT	43	0.10125032376557018	No Hit
GAGGAGGGGAAGATGGTTGCCGTGTAGTATCAAATAAGTTTGCCATTAAA	43	0.10125032376557018	No Hit
GAGGAGGGGATAACTAACATGTAATAAAGGACACGTCAGCCCCTGCCCTG	43	0.10125032376557018	No Hit
GAGGAGGAGGCACATTGACCCGAGTGCATTGCAGGAATGTACTAGGAAAA	43	0.10125032376557018	No Hit
GAGGAGGAAGAAGCTGCCAAGGATGAGTCTGAAGACACAAAAGAAGAATC	43	0.10125032376557018	No Hit
GAGGAGGAAGACAACTGAACATTTGTATAAAACGTAAAAAGTTTACTGAT	43	0.10125032376557018	No Hit
GAGGAGGGCATCCAGAAAGAGTCCACCCTGCACTTAGTCCTTCGCCTCAG	43	0.10125032376557018	No Hit
GAGGAGGGGAGGAGACCCAGAATCTCACGTAACCTTTAAACTTCTTCAGA	43	0.10125032376557018	No Hit
GAGGAGGGGGACTTTGGCGTGGCGTCACACCCGGAAGCAGCCCTGCAGCG	43	0.10125032376557018	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.12244225199557324	0.0	0.0	0.0	0.0
6	0.12244225199557324	0.0	0.0	0.0	0.0
7	0.12244225199557324	0.0	0.0	0.0	0.0
8	0.12244225199557324	0.0	0.0	0.0	0.0
9	0.1247969106877958	0.0	0.0	0.0	0.0
10	0.22369257576114343	0.0	0.0	0.0	0.0
11	0.27784972568226235	0.0	0.0	0.0	0.0
12	0.3131696060656008	0.0	0.0	0.0	0.0
13	0.34848948644893923	0.0	0.0	0.0	0.0
14	0.38616402552450024	0.0	0.0	0.0	0.0
15	0.4002919776778356	0.0	0.0	0.0	0.0
16	0.44973981021450943	0.0	0.0	0.0	0.0
17	0.47328639713673504	0.0	0.0	0.0	0.0
18	0.5156702535967411	0.0	0.0	0.0	0.0
19	0.5627634274411925	0.0	0.0	0.0	0.0
20	0.6051472839011985	0.0	0.0	0.0	0.0
21	0.6593044338223174	0.0	0.0	0.0	0.0
22	0.6969789728978785	0.0	0.0	0.0	0.0
23	0.737008170665662	0.0	0.0	0.0	0.0
24	0.8194212248934517	0.0	0.0	0.0	0.0
25	0.8712237161223481	0.0	0.0	0.0	0.0
26	0.9277355247356895	0.0	0.0	0.0	0.0
27	0.984247333349031	0.0	0.0	0.0	0.0
28	1.054887094115708	0.0	0.0	0.0	0.0
29	1.1019802679601591	0.0	0.0	0.0	0.0
30	1.134945489651275	0.0	0.0	0.0	0.0
31	1.167910711342391	0.0	0.0	0.0	0.0
32	1.1961666156490616	0.0	0.0	0.0	0.0
33	1.2197132025712873	0.0	0.0	0.0	0.0
34	1.2409051308012904	0.0	0.0	0.0	0.0
35	1.2526784242624032	0.0	0.0	0.0	0.0
36	1.280934328569074	0.0	0.0	0.0	0.0
37	1.2997715981068545	0.0	0.0	0.0	0.0
38	1.3350914784901928	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCAAG	30	2.4703731E-6	44.000004	33
CAAGACA	30	2.4703731E-6	44.000004	29
AAACTAC	30	2.4703731E-6	44.000004	27
ATCTCTG	30	2.4703731E-6	44.000004	38
AATCTAA	30	2.4703731E-6	44.000004	21
GCCTCAC	30	2.4703731E-6	44.000004	40
TCAAGGA	30	2.4703731E-6	44.000004	35
TACAGTG	30	2.4703731E-6	44.000004	31
TCAAGAC	30	2.4703731E-6	44.000004	28
CCCAGCG	30	2.4703731E-6	44.000004	12
GGGCAAC	65	0.0	44.000004	7
CTTTAAG	30	2.4703731E-6	44.000004	40
TTCGTGC	30	2.4703731E-6	44.000004	19
TGCAATC	30	2.4703731E-6	44.000004	18
AAATTCA	30	2.4703731E-6	44.000004	21
AGGACTG	30	2.4703731E-6	44.000004	38
TTCGTCA	30	2.4703731E-6	44.000004	31
CCAGCGG	30	2.4703731E-6	44.000004	13
CACGTTC	30	2.4703731E-6	44.000004	27
AGGACGC	30	2.4703731E-6	44.000004	39
>>END_MODULE