FastQCFastQC Report
Fri 17 Jun 2016
SRR1527405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383601
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC248666.482256302772933No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG82242.1438943068448726No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT34800.9071926298419452No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC33350.8693929369318641No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC28820.7513014825300247No Hit
GCTCAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23470.6118336500686912No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC19630.5117296357413041No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT16550.4314378742495457No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT15810.41214699648853886No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGT14790.38555686768282665No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC13090.34123998633997304No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG12470.325077359026697No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTAC11570.3016154806687157No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC10790.2812818527584652No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT10440.2721577889525836No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGC9900.2580806619377948No Hit
GCTCAAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTG9440.24608903522149317No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT8880.23149053313208257No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGC8880.23149053313208257No Hit
GCTCAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG8710.22705884499779722No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC8690.2265374699231754No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAATGGGTAAGAGCACCCGACT8580.22366990701275544No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTG8510.22184509425157908No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTG8250.2150672182814956No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC7730.20151146634132863No Hit
GCTCAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG7250.18899846455040525No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCATCGGTTAAGGGCACTGGCTGC6860.17883165059528No Hit
GCTCAAGGGGAAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACTGACTGT6780.17674615029679278No Hit
GCTCAAGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC6710.17492133753561645No Hit
GCTCAAGGGATTTAAAATTCAGTACCACTTTCTAAAATCTTCCTGGCATA6510.16970758678939837No Hit
GCTCAAGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG6400.16684002387897842No Hit
GCTCAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA5940.15484839716267684No Hit
GCTCAAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAACACTGACTG5710.14885258380452607No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC5690.14833120872990424No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC5620.14650639596872791No Hit
GCTCAAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG5470.14259608290906436No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAACAGTGACTGCT5470.14259608290906436No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGATTGC5360.13972851999864444No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCA5330.1389464573867117No Hit
GCTCAAGGGGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGAGAG5270.1373823321628463No Hit
GCTCAAGGGGGGCTGGCAAGATGGCTCAGCGGTTAAGAGCATTGTCTGCT5170.13477545678973726No Hit
GCTCAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT5160.13451476925242636No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT5120.13347201910318274No Hit
GCTCAAGGGCATGTAGGCGCTCATACCGATGTTCACGTCCACCGCGCCCA5080.1324292689539391No Hit
GCTCAAGGGGGGGGTGGTGGTGAGATGGCTCAGAGGGTAAGAGCACTGAC5060.1319078938793173No Hit
GCTCAAGGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTG4850.12643345559578834No Hit
GCTCAAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGC4830.1259120805211665No Hit
GCTCAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG4730.12330520514805748No Hit
GCTCAAGGGGGGGCTGGAGAGACAGCTCAGTGGTTAAGAGCACTGACTGC4690.12226245499881386No Hit
GCTCAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAG4680.12200176746150297No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCCCCCGACTGC4590.11965557962570483No Hit
GCTCAAGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGC4580.11939489208839392No Hit
GCTCAAGGGACCTGCTGCCTGTGCCTTCCCAGAGGCCAGTGGGGCCTGGC4540.11835214193915032No Hit
GCTCAAGGGGGGTCTGGAGAGATGGCTCAGTGATTAAGAGCACTGACTGC4500.1173093917899067No Hit
GCTCAAGGGGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCT4460.11626664164066308No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTG4370.11392045380486494No Hit
GCTCAAGGGGGGACATTGCTGCTGCTACTTGCAGGGGTGGCCTGCCTCCG4190.10922807813326868No Hit
GCTCAAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA4160.10844601552133597No Hit
GCTCAAGGGGGGAGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTG4140.10792464044671417No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGC4100.10688189029747054No Hit
GCTCAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC4090.10662120276015966No Hit
GCTCAAGGGGCACCTGGGGTTGGAGAGATGGCTCAGTGGGTAAGAGCGCT4060.10583914014822694No Hit
GCTCAAGGGAGTTCATGGCTCAGTTCCATGAAAAGATCTACCAGATGCTG4040.10531776507360512No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCT3990.1040143273870506No Hit
GCTCAAGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGG3980.10375363984973972No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTG3970.1034929523124288No Hit
GCTCAAGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA3920.10218951462587428No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC3920.10218951462587428No Hit
GCTCAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA3840.10010401432738705No Hit
GCTCAAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC3840.10010401432738705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT207.849916E-444.017
CTTCGGT207.849916E-444.024
CGGGTTG207.849916E-444.012
AACGTGC302.5232912E-644.015
TGTCACG302.5232912E-644.042
ATGGGCG254.4372777E-544.012
AGGTACA207.849916E-444.015
CAGCGAA207.849916E-444.024
AACCGAT207.849916E-444.022
GGGTGCG254.4372777E-544.08
CAAACGA207.849916E-444.016
CCGGGAT207.849916E-444.032
ACGCCGT351.4427133E-744.029
GATCGTT207.849916E-444.09
AGTCATT551.8189894E-1244.025
TCCGCAC254.4372777E-544.036
CGTATCC207.849916E-444.037
CAACGTG302.5232912E-644.014
GATCGAA302.5232912E-644.09
GGATGTC207.849916E-444.08
Produced by FastQC (version 0.11.5)