FastQCFastQC Report
Fri 17 Jun 2016
SRR1527404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390078
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC247226.3377068176108375No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT78542.0134434651531232No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC34510.8846948559006147No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC33100.8485482390701347No Hit
GCTCAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT22770.583729407964561No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC18840.48298032701152077No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC16600.425555914458134No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT16330.41863422187357396No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC15950.4088925804582673No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT13760.35274996282794724No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT12770.3273704233512272No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA12580.32249960264357386No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC12330.3160906280282405No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTACTC11450.2935310373822671No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT10920.27994401119776047No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGCTC10230.26225524125944044No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT10140.2599480103979204No Hit
GCTCAAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCT9910.25405175375181377No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTT9280.2379011377211737No Hit
GCTCAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT9060.23226124005968035No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT9050.23200488107506706No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCT9000.23072308615200038No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT8880.22764677833664038No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT8240.211239803321387No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC8060.206625341598347No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTC8020.20559990565989367No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGC7690.19714005916765368No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC7590.1945764693215203No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTA7360.18868021267541363No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCATCGGTTAAGGGCACTGGCTGCTC7240.18560390486005363No Hit
GCTCAAGGGGAAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTC6450.16535154507560026No Hit
GCTCAAGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT6430.1648388271063736No Hit
GCTCAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT6200.1589425704602669No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT6130.15714805756797356No Hit
GCTCAAGGGATTTAAAATTCAGTACCACTTTCTAAAATCTTCCTGGCATAAA5890.1509954419372536No Hit
GCTCAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA5880.15073908295264024No Hit
GCTCAAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAACACTGACTGCT5850.14997000599880025No Hit
GCTCAAGGGGGGCTGGCAAGATGGCTCAGCGGTTAAGAGCATTGTCTGCTCT5840.1497136470141869No Hit
GCTCAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA5840.1497136470141869No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC5710.14638098021421356No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGATTGCTC5650.14484282630653356No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCACT5520.14151015950656023No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC5450.1397156466142669No Hit
GCTCAAGGGCATGTAGGCGCTCATACCGATGTTCACGTCCACCGCGCCCACG5330.13663933879890688No Hit
GCTCAAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5280.1353575438758402No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAACAGTGACTGCTCT5210.13356303098354688No Hit
GCTCAAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTC5160.1322812360604802No Hit
GCTCAAGGGGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGAGAGGC5130.13151215910664021No Hit
GCTCAAGGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCT5010.1284358512912802No Hit
GCTCAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT5010.1284358512912802No Hit
GCTCAAGGGGGGTCTGGAGAGATGGCTCAGTGATTAAGAGCACTGACTGCTC5000.12817949230666686No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCT5000.12817949230666686No Hit
GCTCAAGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4890.1253595434759202No Hit
GCTCAAGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTC4880.12510318449130686No Hit
GCTCAAGGGGGGGGTGGTGGTGAGATGGCTCAGAGGGTAAGAGCACTGACTG4830.1238213895682402No Hit
GCTCAAGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC4670.11971964581442686No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCCCCCGACTGCTC4610.11818149190674686No Hit
GCTCAAGGGGGGGCTGGAGAGACAGCTCAGTGGTTAAGAGCACTGACTGCTC4410.11305431221448017No Hit
GCTCAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4360.1117725172914135No Hit
GCTCAAGGGAGTTCATGGCTCAGTTCCATGAAAAGATCTACCAGATGCTGAA4320.11074708135296017No Hit
GCTCAAGGGGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTT4280.10972164541450684No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCT4240.1086962094760535No Hit
GCTCAAGGGACCTGCTGCCTGTGCCTTCCCAGAGGCCAGTGGGGCCTGGCAG4240.1086962094760535No Hit
GCTCAAGGGGGGAGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT4210.10792713252221352No Hit
GCTCAAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT4190.10741441455298682No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCT4180.1071580555683735No Hit
GCTCAAGGGGGGACATTGCTGCTGCTACTTGCAGGGGTGGCCTGCCTCCGAG4100.10510718369146685No Hit
GCTCAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA4070.10433810673762683No Hit
GCTCAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTG4050.10382538876840017No Hit
GCTCAAGGGCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCAT4020.10305631181456015No Hit
GCTCAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG4020.10305631181456015No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC3990.10228723486072017No Hit
GCTCAAGGGGCTGCTCAAGGGGGGGCGCTGGAGAGATGGCTCAGTGGTTAAG3990.10228723486072017No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTT3950.10126179892226683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG206.306301E-446.00000444
AAATCTA206.306301E-446.00000414
CGAACTA206.306301E-446.00000427
GTCGAGA206.306301E-446.00000431
GATCGGT405.5952114E-946.00000436
GTCGAAT206.306301E-446.00000419
GGTCGCC206.306301E-446.0000049
ATAGGAT351.0172516E-746.00000413
CCGATTA206.306301E-446.00000444
AGATAGG351.0172516E-746.00000411
TGCCACG206.306301E-446.00000439
CCGATGT405.5952114E-946.00000426
GCGGTCT206.306301E-446.00000423
ATAATAG351.0172516E-746.00000418
CTGAGCG351.0172516E-746.00000420
CGCATGA206.306301E-446.00000416
CGTTGGA206.306301E-446.00000428
CGGCACA206.306301E-446.00000424
GACCGCG206.306301E-446.0000049
CCGGATA206.306301E-446.00000411
Produced by FastQC (version 0.11.5)