FastQCFastQC Report
Fri 17 Jun 2016
SRR1527403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345780
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC213046.161142923245994No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT63651.8407658048470124No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC27280.7889409451096072No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC25140.7270518826999827No Hit
GCTCAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT19010.5497715310312915No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC17280.4997397188964081No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT14500.4193417780091388No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC14060.40661692405575794No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC13810.39938689340042804No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT12000.34704147145583897No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC11190.3236161721325698No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT10780.31175892185782866No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA10340.2990340679044479No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT10240.2961420556423159No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTACTC9850.28486320782000113No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT9810.28370640291514837No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGCTC8910.2576782925559604No Hit
GCTCAAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCT8150.23569899936375732No Hit
GCTCAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT7990.2310717797443461No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCT7900.2284689687084273No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT7840.22673376135114812No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTT7680.22210654173173694No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT7620.22037133437445774No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTC7340.21227370004048818No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGC6900.1995488460871074No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC6780.19607843137254902No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC6720.19434322401526982No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTA6650.19231881543177742No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT6640.19202961420556425No Hit
GCTCAAGGGGAAGCTGGCGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTC6460.18682399213372666No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCATCGGTTAAGGGCACTGGCTGCTC6200.17930476025218345No Hit
GCTCAAGGGATTTAAAATTCAGTACCACTTTCTAAAATCTTCCTGGCATAAA6180.17872635779975707No Hit
GCTCAAGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT5930.17149632714442709No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC5630.1628202903580311No Hit
GCTCAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5500.15906067441725955No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCACT5370.15530105847648795No Hit
GCTCAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA5360.15501185725027472No Hit
GCTCAAGGGCATGTAGGCGCTCATACCGATGTTCACGTCCACCGCGCCCACG5230.15125224130950315No Hit
GCTCAAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAACACTGACTGCT5180.14980623517843714No Hit
GCTCAAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC5130.14836022904737117No Hit
GCTCAAGGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCT4980.14402221065417317No Hit
GCTCAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA4970.14373300942795997No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT4900.1417086008444676No Hit
GCTCAAGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAACAGTGACTGCTCT4630.1339001677367112No Hit
GCTCAAGGGGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGAGAGGC4530.13100815547457922No Hit
GCTCAAGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4450.12869454566487362No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGATTGCTC4410.12753774076002083No Hit
GCTCAAGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTC4400.1272485395338076No Hit
GCTCAAGGGACCTGCTGCCTGTGCCTTCCCAGAGGCCAGTGGGGCCTGGCAG4360.12609173462895484No Hit
GCTCAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT4360.12609173462895484No Hit
GCTCAAGGGGGGCTGGCAAGATGGCTCAGCGGTTAAGAGCATTGTCTGCTCT4320.12493492972410203No Hit
GCTCAAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4310.12464572849788882No Hit
GCTCAAGGGGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTT4300.12435652727167563No Hit
GCTCAAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTC4240.12262131991439644No Hit
GCTCAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4150.12001850887847765No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCT4100.11857250274741164No Hit
GCTCAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA4040.11683729539013245No Hit
GCTCAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTG4020.11625889293770605No Hit
GCTCAAGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC3990.11539128925906646No Hit
GCTCAAGGGGGGGGTGGTGGTGAGATGGCTCAGAGGGTAAGAGCACTGACTG3960.11452368558042686No Hit
GCTCAAGGGATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAATA3940.11394528312800047No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCT3820.11047486841344208No Hit
GCTCAAGGGAGTTCATGGCTCAGTTCCATGAAAAGATCTACCAGATGCTGAA3810.11018566718722887No Hit
GCTCAAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA3750.10845045982994968No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC3720.10758285615131008No Hit
GCTCAAGGGGGGTCTGGAGAGATGGCTCAGTGATTAAGAGCACTGACTGCTC3710.10729365492509688No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCCCCCGACTGCTC3690.10671525247267048No Hit
GCTCAAGGGGGCACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCT3690.10671525247267048No Hit
GCTCAAGGGGCTGCTCAAGGGGGGGCGCTGGAGAGATGGCTCAGTGGTTAAG3680.10642605124645728No Hit
GCTCAAGGGGGGAGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT3560.10295563653189889No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTT3540.1023772340794725No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTT3480.1006420267221933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATG351.0167969E-746.00000412
ACGTTGC351.0167969E-746.00000446
ACGCAGG351.0167969E-746.00000438
AAGAACG351.0167969E-746.00000416
CGTTAGT351.0167969E-746.00000421
AACGGGT351.0167969E-746.00000420
CTAACGT351.0167969E-746.00000426
TATTCTA351.0167969E-746.00000440
TCTAACG351.0167969E-746.00000425
TACCTCC351.0167969E-746.00000433
ATGACGC351.0167969E-746.00000426
GCTAGAT351.0167969E-746.00000411
GATTGGG351.0167969E-746.00000425
CGCAACG351.0167969E-746.00000417
AATGTCG351.0167969E-746.00000433
ATCGAAG351.0167969E-746.00000410
CTTCGTA206.3053035E-446.022
TATCATA206.3053035E-446.041
CTTCGGC405.5915734E-946.017
CTTCGCA206.3053035E-446.042
Produced by FastQC (version 0.11.5)