FastQCFastQC Report
Fri 17 Jun 2016
SRR1527402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480458
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA49181.0236066419957623No Hit
AACCTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA22290.4639323312339476No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC20500.42667621311332105No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG15710.3269796735614768No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG15460.3217763051088753No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT13990.2911804986075786No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG12790.26620433003509153No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA11350.236232927748107No Hit
AACCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT11020.22936448139067306No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG10830.22540992136669594No Hit
AACCTAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAA10130.21084048969941183No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA9580.1993930791036886No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC9560.19897680962748043No Hit
AACCTAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC8750.18211789584105167No Hit
AACCTAGGGGGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGGCG8180.1702542157691203No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8120.16900540734049596No Hit
AACCTAGGGGGCATGATTCAGACTGGATACTCTCAGTGCCAGCCAAATTA7470.15547664936373212No Hit
AACCTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT7280.15152208933975497No Hit
AACCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT7210.15006514617302658No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC7020.14611058614904945No Hit
AACCTAGGGGCCCTAGCGACCTGTGCCGGGATCTTCGGAGCCGCAACCGA6850.14257229560128043No Hit
AACCTAGGGAAAGATGATCGAGAGGGTCGATTCGTCCTTAAGAACGAGAA6720.13986654400592766No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC6620.13778519662488709No Hit
AACCTAGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTG6410.13341436712470187No Hit
AACCTAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC6360.13237369343418157No Hit
AACCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC6250.1300842113150369No Hit
AACCTAGGGATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTT6230.1296679418388288No Hit
AACCTAGGGGCTGGGCAGTGGTGGCGCACGCCTTTAATCCCAGTACATGG6230.1296679418388288No Hit
AACCTAGGGGAGCGCGGCTAGAATCTGCCAACGAGTCGCGTAGTAGCCCT5870.12217509126708267No Hit
AACCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC5850.12175882179087455No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG5840.12155068705277049No Hit
AACCTAGGGGTCCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGACC5750.11967747440983394No Hit
AACCTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA5610.11676358807637711No Hit
AACCTAGGGGAGCTCTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGT5500.11447410595723248No Hit
AACCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG5420.1128090280524No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCT5370.11176835436187971No Hit
AACCTAGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA5300.11031141119515131No Hit
AACCTAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGC5150.1071893901235904No Hit
AACCTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC5060.10531617748065387No Hit
AACCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG5040.10489990800444575No Hit
AACCTAGGGAGTGCAATATGCCCTCCCCGTGGGGTCCCTTGGCACGGTAC4870.10136161745667674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA1050.044.015
CGGCGTA207.8519026E-444.038
ACCGGAT207.8519026E-444.038
CATATCG454.783942E-1044.025
CTCAAAT207.8519026E-444.039
GGTAACG207.8519026E-444.09
GGTAAAC207.8519026E-444.010
CGAAATA207.8519026E-444.012
ATAGGCT600.044.012
GGTCGAC254.43896E-544.09
CTATAGA207.8519026E-444.034
CCAATCG254.43896E-544.014
CCTACGC207.8519026E-444.044
TTGTACG254.43896E-544.037
AAGCGTG254.43896E-544.015
TACGGGT207.8519026E-444.018
TACGGAT454.783942E-1044.013
TCGATGG408.294592E-944.018
ACTTACA254.43896E-544.026
TCGATAT207.8519026E-444.015
Produced by FastQC (version 0.11.5)