##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480458
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.439786620266496	31.0	30.0	33.0	28.0	34.0
2	31.137860125130604	31.0	31.0	33.0	28.0	34.0
3	32.01035054052591	33.0	31.0	34.0	30.0	34.0
4	36.2278575858868	37.0	35.0	37.0	35.0	37.0
5	36.29327641542029	37.0	37.0	37.0	35.0	37.0
6	35.416683664336944	36.0	35.0	37.0	33.0	37.0
7	36.34693771359827	37.0	36.0	37.0	35.0	37.0
8	36.69166295493051	37.0	37.0	37.0	35.0	37.0
9	38.70162636484355	39.0	39.0	39.0	39.0	39.0
10	37.59198306615771	39.0	37.0	39.0	35.0	39.0
11	37.452849156429906	39.0	37.0	39.0	35.0	39.0
12	37.25443014790054	39.0	37.0	39.0	35.0	39.0
13	37.24333864770698	39.0	37.0	39.0	35.0	39.0
14	38.05814452043675	40.0	38.0	40.0	34.0	40.0
15	38.076412506400146	40.0	38.0	40.0	34.0	40.0
16	38.21547356896961	40.0	38.0	40.0	35.0	40.0
17	38.14443093881255	40.0	38.0	40.0	35.0	40.0
18	37.99461555432525	40.0	38.0	40.0	34.0	40.0
19	37.940013487131026	40.0	38.0	40.0	34.0	40.0
20	37.77272727272727	39.0	37.0	40.0	34.0	40.0
21	37.65016296949994	39.0	37.0	40.0	34.0	40.0
22	37.46031286813832	39.0	36.0	40.0	34.0	40.0
23	37.24589662363828	38.0	36.0	40.0	33.0	40.0
24	37.107934512486004	38.0	36.0	40.0	33.0	40.0
25	36.852159814177305	38.0	36.0	40.0	33.0	40.0
26	36.315817407556956	38.0	35.0	39.0	32.0	40.0
27	36.17729749530656	38.0	35.0	39.0	31.0	40.0
28	35.99340004745472	38.0	35.0	39.0	31.0	40.0
29	35.601224248529526	37.0	34.0	38.0	31.0	40.0
30	35.294373285490096	37.0	34.0	38.0	30.0	40.0
31	35.08254831847945	36.0	34.0	38.0	30.0	40.0
32	34.964192499656576	36.0	33.0	38.0	30.0	39.0
33	34.47728001198856	36.0	33.0	38.0	29.0	39.0
34	34.141338056604326	36.0	33.0	38.0	28.0	39.0
35	33.96550166715925	35.0	32.0	38.0	28.0	39.0
36	33.535455752636025	35.0	32.0	38.0	27.0	38.0
37	33.211466975261104	34.0	31.0	37.0	27.0	38.0
38	32.81731181497654	34.0	31.0	37.0	26.0	38.0
39	32.06192216593334	34.0	30.0	36.0	24.0	38.0
40	31.905423575005514	33.0	30.0	36.0	24.0	38.0
41	31.478553796585757	33.0	29.0	36.0	23.0	38.0
42	31.084560981396915	33.0	29.0	35.0	23.0	38.0
43	30.65502707832943	32.0	28.0	35.0	23.0	37.0
44	30.05646903579501	31.0	27.0	35.0	21.0	37.0
45	29.602331525336243	31.0	27.0	34.0	21.0	37.0
46	29.530029680013655	31.0	27.0	34.0	21.0	37.0
47	28.93969712232911	31.0	26.0	34.0	20.0	36.0
48	28.342827052520718	30.0	25.0	33.0	19.0	36.0
49	27.869655620262332	29.0	24.0	33.0	18.0	35.0
50	31.673166020755197	33.0	30.0	36.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	7.0
15	12.0
16	37.0
17	73.0
18	130.0
19	187.0
20	321.0
21	442.0
22	665.0
23	1048.0
24	1576.0
25	2292.0
26	3263.0
27	4487.0
28	6168.0
29	8639.0
30	12560.0
31	17969.0
32	27691.0
33	39737.0
34	63075.0
35	135065.0
36	136300.0
37	18226.0
38	486.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.18140607503673	0.7405433981742421	0.044748968692372694	0.03330155809664945
8	99.3387559370434	0.4945281377352443	0.12841913341020442	0.03829679181114686
9	98.46417376753016	0.8516873483218095	0.23748173617673135	0.4466571479713107
10	60.860886903746014	31.819638761348546	2.520511678440155	4.79896265646529
11	35.04947362724734	26.8075461330647	21.830836410258545	16.31214382942942
12	29.630477585970056	19.246427367220445	25.79621944061708	25.326875606192424
13	24.031236861494655	18.469668524616097	29.191729558046696	28.30736505584255
14	25.71900145278047	18.96690241394669	28.931561135416622	26.38253499785621
15	26.33965924180678	19.277855712674157	26.88622106406803	27.49626398145103
16	29.661073392471348	20.320194481099286	24.971173338772587	25.047558787656776
17	29.603628204754628	21.102364826894338	23.372490415395312	25.92151655295572
18	29.751820138284717	20.001748331800073	22.178629557630426	28.067801972284776
19	27.336832772063325	20.120176997781282	24.024368415137225	28.518621815018168
20	27.63550612124265	21.780467803637364	24.130517131570294	26.453508943549696
21	28.52486585716129	19.81172131591107	25.61243646687119	26.050976360056445
22	29.43337398898551	19.632517306403475	24.815280419932648	26.11882828467837
23	29.161966290497816	22.006502129218372	22.890242227208205	25.941289353075607
24	27.118499431792166	22.11452405829438	23.371657876442896	27.395318633470563
25	26.777366596039613	21.68951292308589	21.85227428828326	29.680846192591236
26	26.54862651886325	23.50340716566276	22.721028685129603	27.226937630344377
27	27.097477823243654	20.17845472445042	24.84004845376703	27.884018998538895
28	28.389162007917445	21.234530385590418	22.08351198231687	28.292795624175266
29	28.15063959805019	20.57432699632434	23.212851071269498	28.062182334355967
30	27.453388225401596	20.32914427483776	24.965761835581883	27.25170566417876
31	29.728925317093275	21.37876775909653	21.685142093585704	27.207164830224496
32	29.010652335896165	22.511645138596922	22.801576828775875	25.676125696731035
33	31.14382526672467	20.02256180561048	22.812816104633495	26.020796823031354
34	29.396326005602987	21.24826727830528	25.289411353333698	24.065995362758034
35	28.24887919443531	23.807075748556585	24.947029709152517	22.997015347855587
36	29.297045735527348	21.569211044461742	21.997344200741793	27.136399019269113
37	27.538099063809945	24.265596576599826	23.22929371557972	24.967010644010507
38	30.539610122008582	22.373859941972036	23.22159273026987	23.864937205749513
39	29.3869599423883	20.731884993069112	21.559220577032747	28.321934487509836
40	27.964983411661372	22.42027398856924	22.092461776055348	27.52228082371404
41	25.384112659171045	23.816025542295062	22.890242227208205	27.909619571325695
42	26.269934104541914	21.427263153074776	22.845076989039626	29.457725753343684
43	26.606904245532387	20.363278371886825	25.189090409567537	27.840726973013254
44	26.666638915368253	19.504930711945683	25.703807616898878	28.124622755787186
45	27.03982450078883	20.405737858460054	24.97637670722519	27.578060933525926
46	29.06185348146976	22.58990380012405	22.57658317688539	25.771659541520798
47	26.009349412435633	22.88441445454129	24.010215252946146	27.096020880076928
48	27.59013274833596	22.179045827106634	23.578960075594537	26.651861348962864
49	27.65465451714822	22.20381386094102	23.572299763975206	26.569231857935556
50	24.784268343955144	24.13343101790375	24.86564902655383	26.216651611587277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	2.0
8	3.0
9	49.5
10	96.0
11	325.5
12	555.0
13	634.5
14	714.0
15	619.5
16	525.0
17	491.0
18	457.0
19	459.5
20	462.0
21	554.5
22	647.0
23	679.5
24	712.0
25	1329.5
26	1947.0
27	2135.0
28	2323.0
29	2939.0
30	3555.0
31	4098.0
32	4641.0
33	5222.5
34	5804.0
35	7047.5
36	8291.0
37	8734.5
38	9178.0
39	9773.5
40	10369.0
41	12183.5
42	13998.0
43	14207.0
44	14416.0
45	15887.0
46	17358.0
47	20136.5
48	22915.0
49	24447.0
50	25979.0
51	27101.0
52	28223.0
53	30190.0
54	32157.0
55	35880.0
56	39603.0
57	44177.5
58	48752.0
59	48406.5
60	48061.0
61	43454.0
62	38847.0
63	35423.5
64	32000.0
65	27193.0
66	22386.0
67	18299.0
68	14212.0
69	14828.0
70	15444.0
71	10997.0
72	6550.0
73	5833.5
74	5117.0
75	3819.0
76	2521.0
77	1560.5
78	600.0
79	562.0
80	524.0
81	475.5
82	427.0
83	255.0
84	83.0
85	43.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	480458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.233864354428484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3097787823767	7.898505176310937
2	9.197020787786713	2.0663616798970983
3	3.3071478860191945	1.1145615225472363
4	1.4006743987846	0.6293994480266746
5	0.7188646385296624	0.40378139192187457
6	0.46874421017526957	0.31594853244196164
7	0.3983399414532923	0.31324278084660884
8	0.31311372142142513	0.2813981659166878
9	0.30570274576648016	0.3090800860845277
>10	9.300774446955943	29.108267528066968
>50	2.877311298032386	21.880372477927313
>100	1.3284173861488864	27.689829287887807
>500	0.05372957349835106	3.967048108263365
>1k	0.020380183051098677	4.022203813860941
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACCTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA	4918	1.0236066419957623	No Hit
AACCTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA	2229	0.4639323312339476	No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2050	0.42667621311332105	No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1571	0.3269796735614768	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1546	0.3217763051088753	No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1399	0.2911804986075786	No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1279	0.26620433003509153	No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	1135	0.236232927748107	No Hit
AACCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1102	0.22936448139067306	No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	1083	0.22540992136669594	No Hit
AACCTAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAA	1013	0.21084048969941183	No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	958	0.1993930791036886	No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	956	0.19897680962748043	No Hit
AACCTAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC	875	0.18211789584105167	No Hit
AACCTAGGGGGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGGCG	818	0.1702542157691203	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	812	0.16900540734049596	No Hit
AACCTAGGGGGCATGATTCAGACTGGATACTCTCAGTGCCAGCCAAATTA	747	0.15547664936373212	No Hit
AACCTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	728	0.15152208933975497	No Hit
AACCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	721	0.15006514617302658	No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	702	0.14611058614904945	No Hit
AACCTAGGGGCCCTAGCGACCTGTGCCGGGATCTTCGGAGCCGCAACCGA	685	0.14257229560128043	No Hit
AACCTAGGGAAAGATGATCGAGAGGGTCGATTCGTCCTTAAGAACGAGAA	672	0.13986654400592766	No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	662	0.13778519662488709	No Hit
AACCTAGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTG	641	0.13341436712470187	No Hit
AACCTAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	636	0.13237369343418157	No Hit
AACCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	625	0.1300842113150369	No Hit
AACCTAGGGATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTT	623	0.1296679418388288	No Hit
AACCTAGGGGCTGGGCAGTGGTGGCGCACGCCTTTAATCCCAGTACATGG	623	0.1296679418388288	No Hit
AACCTAGGGGAGCGCGGCTAGAATCTGCCAACGAGTCGCGTAGTAGCCCT	587	0.12217509126708267	No Hit
AACCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	585	0.12175882179087455	No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	584	0.12155068705277049	No Hit
AACCTAGGGGTCCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGACC	575	0.11967747440983394	No Hit
AACCTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	561	0.11676358807637711	No Hit
AACCTAGGGGAGCTCTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGT	550	0.11447410595723248	No Hit
AACCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	542	0.1128090280524	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCT	537	0.11176835436187971	No Hit
AACCTAGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA	530	0.11031141119515131	No Hit
AACCTAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGC	515	0.1071893901235904	No Hit
AACCTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	506	0.10531617748065387	No Hit
AACCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	504	0.10489990800444575	No Hit
AACCTAGGGAGTGCAATATGCCCTCCCCGTGGGGTCCCTTGGCACGGTAC	487	0.10136161745667674	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.03080394123940074	0.0	0.0	0.0	0.0
7	0.031220210715608855	0.0	0.0	0.0	0.0
8	0.031220210715608855	0.0	0.0	0.0	0.0
9	0.03142834545371292	0.0	0.0	0.0	0.0
10	0.0401700044540834	0.0	0.0	0.0	0.0
11	0.05411503190705535	0.0	0.0	0.0	0.0
12	0.06368922985984207	0.0	0.0	0.0	0.0
13	0.06868446357433948	0.0	0.0	0.0	0.0
14	0.08054814364627084	0.0	0.0	0.0	0.0
15	0.08908166790853728	0.0	0.0	0.0	0.0
16	0.09595011426597122	0.0	0.0	0.0	0.0
17	0.10073721324236458	0.0	0.0	0.0	0.0
18	0.10656498590927824	0.0	0.0	0.0	0.0
19	0.11572291438585683	0.0	0.0	0.0	0.0
20	0.12155068705277049	0.0	0.0	0.0	0.0
21	0.12821099867210037	0.0	0.0	0.0	0.0
22	0.13383063660090996	0.0	0.0	0.0	0.0
23	0.1427804303393845	0.0	0.0	0.0	0.0
24	0.15027328091113062	0.0	0.0	0.0	0.0
25	0.15734986200666864	0.0	0.0	0.0	0.0
26	0.1660915210070391	0.0	0.0	0.0	0.0
27	0.17337623684068118	0.0	0.0	0.0	0.0
28	0.1910676895795262	0.0	0.0	0.0	0.0
29	0.19897680962748043	0.0	0.0	0.0	0.0
30	0.20771846862785093	0.0	0.0	0.0	0.0
31	0.21895774448547012	0.0	0.0	0.0	0.0
32	0.22749126874773654	0.0	0.0	0.0	0.0
33	0.23352717615275426	0.0	0.0	0.0	0.0
34	0.2424769698912288	0.0	0.0	0.0	0.0
35	0.2576708057728251	0.0	0.0	0.0	0.0
36	0.2674531384637159	0.0	0.0	0.0	0.0
37	0.27557039324977417	0.0	0.0	0.0	0.0
38	0.28535272594066496	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	105	0.0	44.0	15
CGGCGTA	20	7.8519026E-4	44.0	38
ACCGGAT	20	7.8519026E-4	44.0	38
CATATCG	45	4.783942E-10	44.0	25
CTCAAAT	20	7.8519026E-4	44.0	39
GGTAACG	20	7.8519026E-4	44.0	9
GGTAAAC	20	7.8519026E-4	44.0	10
CGAAATA	20	7.8519026E-4	44.0	12
ATAGGCT	60	0.0	44.0	12
GGTCGAC	25	4.43896E-5	44.0	9
CTATAGA	20	7.8519026E-4	44.0	34
CCAATCG	25	4.43896E-5	44.0	14
CCTACGC	20	7.8519026E-4	44.0	44
TTGTACG	25	4.43896E-5	44.0	37
AAGCGTG	25	4.43896E-5	44.0	15
TACGGGT	20	7.8519026E-4	44.0	18
TACGGAT	45	4.783942E-10	44.0	13
TCGATGG	40	8.294592E-9	44.0	18
ACTTACA	25	4.43896E-5	44.0	26
TCGATAT	20	7.8519026E-4	44.0	15
>>END_MODULE