FastQCFastQC Report
Fri 17 Jun 2016
SRR1527401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423577
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG44361.047271216331387No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA19900.46980832292593794No Hit
AACCTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG19420.458476262875463No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG14230.33594836357970337No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT13900.32815757229500186No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG12720.30029959133758444No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12270.2896757850402642No Hit
AACCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC11380.2686642570300087No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC10330.2438753756695949No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA10050.23726500730681788No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG8990.21224004136201918No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC8760.20681009592116664No Hit
AACCTAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC7980.18839549833914493No Hit
AACCTAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCAC7520.17753560745743985No Hit
AACCTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG7420.1751747616135909No Hit
AACCTAGGGGGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGGCGCC7000.1652592090694254No Hit
AACCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC6980.16478703990065563No Hit
AACCTAGGGGGCATGATTCAGACTGGATACTCTCAGTGCCAGCCAAATTACC6880.16242619405680667No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC6570.155107571940875No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT6560.1548714873564901No Hit
AACCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG6500.15345497985018072No Hit
AACCTAGGGGCCCTAGCGACCTGTGCCGGGATCTTCGGAGCCGCAACCGACC6450.15227455692825625No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT6330.14944154191563755No Hit
AACCTAGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGAT5940.1402342431246267No Hit
AACCTAGGGAAAGATGATCGAGAGGGTCGATTCGTCCTTAAGAACGAGAATG5830.13763731269639287No Hit
AACCTAGGGGCTGGGCAGTGGTGGCGCACGCCTTTAATCCCAGTACATGGGA5690.13433212851500434No Hit
AACCTAGGGGTCCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGACCTG5520.13031869058046117No Hit
AACCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC5470.1291382676585367No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG5390.12724959098345756No Hit
AACCTAGGGGAGCGCGGCTAGAATCTGCCAACGAGTCGCGTAGTAGCCCTAT5240.12370832221768416No Hit
AACCTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG5190.12252789929575968No Hit
AACCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA5110.12063922262068054No Hit
AACCTAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG5060.11945879969875606No Hit
AACCTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC4970.11733403843929202No Hit
AACCTAGGGGAGCTCTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCA4970.11733403843929202No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCT4860.11473710801105821No Hit
AACCTAGGGATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCC4730.1116680084140546No Hit
AACCTAGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT4710.1111958392452848No Hit
AACCTAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCGG4690.11072367007651501No Hit
AACCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC4690.11072367007651501No Hit
AACCTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA4640.10954324715459056No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT4510.10647414755758694No Hit
AACCTAGGGAAGAGCTGCGTCTAGAGACTCCGCTGGGTCAGGCTGAGGGTGC4340.10246070962304375No Hit
AACCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA4270.1008081175323495No Hit
AACCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA4270.1008081175323495No Hit
AACCTAGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCG4260.10057203294796459No Hit
AACCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT4250.10033594836357972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATATC206.3069176E-446.00000417
ATAGGAC206.3069176E-446.00000431
CGCATTG206.3069176E-446.00000418
TAACGGC405.5970304E-946.00000435
ATACTAA206.3069176E-446.00000437
AACGGTT206.3069176E-446.00000446
TGGTTAC206.3069176E-446.00000411
ACTAGCA206.3069176E-446.00000441
GTTCGAT206.3069176E-446.00000413
CGAATTA206.3069176E-446.00000426
CATACGA206.3069176E-446.00000417
CATACCG206.3069176E-446.00000427
TCCGTAT206.3069176E-446.00000423
TTAGTCT206.3069176E-446.00000420
TTAGTAC206.3069176E-446.00000420
TTCGGGA206.3069176E-446.00000411
TAAGGTA405.5970304E-946.00000434
ATACGAT206.3069176E-446.00000418
AATACTA206.3069176E-446.00000436
TCGAATT206.3069176E-446.00000442
Produced by FastQC (version 0.11.5)