##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527401_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423577
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.792854663969006	31.0	31.0	34.0	30.0	34.0
2	32.337183085956035	33.0	31.0	34.0	31.0	34.0
3	32.768528508393985	34.0	31.0	34.0	31.0	34.0
4	36.59179322767761	37.0	37.0	37.0	35.0	37.0
5	36.54694423918201	37.0	37.0	37.0	35.0	37.0
6	35.85744976710256	37.0	35.0	37.0	35.0	37.0
7	36.53450022073908	37.0	37.0	37.0	35.0	37.0
8	36.757522245069964	37.0	37.0	37.0	36.0	37.0
9	38.79573961759019	39.0	39.0	39.0	39.0	39.0
10	37.98505112411675	39.0	38.0	39.0	35.0	39.0
11	37.920807787013935	39.0	37.0	39.0	35.0	39.0
12	37.7551661209178	39.0	37.0	39.0	35.0	39.0
13	37.74799151039834	39.0	37.0	39.0	35.0	39.0
14	38.52383391921658	40.0	39.0	40.0	35.0	40.0
15	38.622680173852686	40.0	39.0	40.0	35.0	40.0
16	38.748631299622026	40.0	39.0	40.0	35.0	40.0
17	38.710114099679636	40.0	39.0	40.0	35.0	40.0
18	38.620040748199266	40.0	38.0	40.0	35.0	40.0
19	38.556368735790656	40.0	38.0	40.0	35.0	40.0
20	38.51565122752179	40.0	38.0	40.0	35.0	40.0
21	38.480417964148195	40.0	38.0	40.0	35.0	40.0
22	38.472405253354175	40.0	38.0	40.0	35.0	40.0
23	38.37284838411906	40.0	38.0	40.0	35.0	40.0
24	38.32212088947228	40.0	38.0	40.0	35.0	40.0
25	38.31202591264398	40.0	38.0	40.0	35.0	40.0
26	38.25547893299211	40.0	38.0	40.0	35.0	40.0
27	38.208068426755936	40.0	38.0	40.0	35.0	40.0
28	38.116531350852384	40.0	38.0	40.0	35.0	40.0
29	38.0982749299419	40.0	38.0	40.0	34.0	40.0
30	37.98053246517162	40.0	38.0	40.0	34.0	40.0
31	37.957561435110975	40.0	38.0	40.0	34.0	40.0
32	37.844769664075244	40.0	38.0	40.0	34.0	40.0
33	37.62504338054238	40.0	37.0	40.0	33.0	40.0
34	37.36884675041374	40.0	36.0	40.0	33.0	40.0
35	37.249279351806166	39.0	36.0	40.0	33.0	40.0
36	37.222339739882	39.0	36.0	40.0	33.0	40.0
37	37.03220665900179	39.0	35.0	40.0	32.0	40.0
38	37.16667807742158	39.0	36.0	40.0	33.0	40.0
39	37.169241956008	39.0	36.0	40.0	33.0	40.0
40	36.978407703912154	39.0	35.0	40.0	32.0	40.0
41	36.85698468047132	39.0	35.0	40.0	32.0	40.0
42	36.757453780540494	39.0	35.0	40.0	32.0	40.0
43	36.71482162629227	39.0	35.0	40.0	32.0	40.0
44	36.59478678020761	39.0	35.0	40.0	31.0	40.0
45	36.47873704190737	39.0	35.0	40.0	31.0	40.0
46	36.32762166028845	38.0	35.0	40.0	31.0	40.0
47	36.16167072338678	38.0	35.0	40.0	31.0	40.0
48	36.02146717125812	38.0	35.0	40.0	31.0	40.0
49	35.83863146488124	37.0	35.0	40.0	30.0	40.0
50	36.10053898110615	38.0	35.0	40.0	31.0	40.0
51	36.1861479730958	37.0	35.0	40.0	32.0	40.0
52	35.869471194139436	37.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	5.0
16	9.0
17	17.0
18	27.0
19	55.0
20	113.0
21	196.0
22	288.0
23	458.0
24	657.0
25	998.0
26	1355.0
27	1967.0
28	2345.0
29	2959.0
30	4058.0
31	5443.0
32	7817.0
33	11273.0
34	23409.0
35	24540.0
36	39218.0
37	72014.0
38	174096.0
39	50255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.23012817032087	0.7143919523486875	0.026441473451108064	0.029038403879341892
8	99.3592664379794	0.4986106422208949	0.1111958392452848	0.03092708055442104
9	98.51243103379078	0.8444745583447637	0.2150730563746379	0.4280213514898118
10	61.209414108886925	31.42569119664193	2.50296876364864	4.861925930822495
11	35.01842640181124	26.676141527986648	21.842309662705954	16.463122407496158
12	29.52025251607146	19.111755359710276	25.900367583697886	25.46762454052038
13	24.039076720407387	18.451662861770117	29.345549923626635	28.16371049419586
14	26.06491853901416	18.651862589328505	28.8835323919854	26.399686479671935
15	26.259688321131698	19.091452085453177	26.988717517712246	27.660142075702883
16	29.617991534006805	20.128335580071628	25.02425769104555	25.22941519487602
17	29.685511725140884	20.797163207634032	23.539521739848954	25.97780332737613
18	29.850062680457157	19.81103789865833	22.34375331993947	27.995146100945046
19	27.590969292478107	20.029416139214355	23.73381935279772	28.64579521550981
20	27.89882359051601	21.53233060340859	24.112026856982318	26.45681894909308
21	28.695845147399414	19.59147923518038	25.4938299293871	26.21884568803311
22	29.504907018086442	19.525139466968223	24.743789204796293	26.22616431014904
23	29.273780209973626	21.856474737769048	22.865736336014468	26.004008716242854
24	27.272963357311657	21.70939404169726	23.499623445087906	27.51801915590318
25	26.8409285678873	21.74527889852376	21.88763790290785	29.526154630681077
26	26.67401676672718	23.173826718636754	22.640983811680048	27.511172702956017
27	27.322777204616873	20.20128571664656	24.786992683738728	27.68894439499784
28	28.532710699589448	20.928662321136418	22.170939404169726	28.367687575104412
29	28.314096374449036	20.38802862289501	23.213725013397802	28.08414998925815
30	27.655420384015184	20.06223189644386	24.82358579431839	27.458761925222568
31	29.714077959851455	21.299079034036314	21.658635856054506	27.32820715005772
32	29.221841601408954	22.29370338804987	22.692450251075954	25.79200475946522
33	31.11099044565687	19.892014911102347	22.717475217020755	26.279519426220027
34	29.77404344428522	21.17159335846847	25.14277215240676	23.911591044839543
35	28.457871886339436	23.55179813823697	24.8811904329083	23.10913954251529
36	29.54504139743187	21.579311435701182	21.79202364623197	27.083623520634976
37	27.789752512530193	24.087710144790677	22.985195135713223	25.13734220696591
38	30.62182318681137	22.136943224018303	23.146204822263723	24.095028766906605
39	29.56841377128598	20.406443220477033	21.50045918451663	28.524683823720366
40	28.235716292433256	22.45093572125021	21.798397930010363	27.514950056306176
41	25.508939342787734	23.51969063476062	22.959225831430885	28.01214419102076
42	26.76656192380606	21.051190220432176	22.76823340266351	29.414014453098257
43	26.911045689449615	20.06270406561263	25.166852780014022	27.859397464923735
44	26.78639302889439	19.306052972659042	25.76296635558588	28.144587642860685
45	27.011853806981968	20.181218526973844	24.866081019507668	27.94084664653652
46	29.296208245490195	22.274580536714694	22.59659990981569	25.832611307979427
47	26.06208552400154	22.710628764073594	24.113679449073015	27.113606262851853
48	27.90118443635986	21.82956109514917	23.682825082570584	26.586429385920386
49	27.828470384369314	21.802647452529293	23.598778970529562	26.77010319257184
50	24.822641455980847	23.97651430554539	24.93667031023875	26.264173928235007
51	25.70087610989265	24.52989657134358	22.333601682810915	27.43562563595285
52	28.52987768457683	22.588100864777832	22.340920304926847	26.54110114571849
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	0.0
7	1.0
8	2.0
9	43.0
10	84.0
11	275.0
12	466.0
13	555.0
14	519.0
15	394.0
16	416.5
17	439.0
18	377.0
19	315.0
20	355.0
21	395.0
22	507.5
23	620.0
24	729.0
25	838.0
26	1359.0
27	1880.0
28	2184.5
29	2489.0
30	2697.0
31	2905.0
32	3886.0
33	4867.0
34	4990.5
35	5114.0
36	5845.5
37	6577.0
38	7372.0
39	9039.0
40	9911.0
41	10133.0
42	10355.0
43	11312.5
44	12270.0
45	13970.0
46	15670.0
47	17013.5
48	18357.0
49	20102.5
50	21848.0
51	22348.5
52	22849.0
53	25421.5
54	27994.0
55	30947.5
56	33901.0
57	37844.5
58	41788.0
59	40857.0
60	39926.0
61	37087.0
62	34248.0
63	31292.0
64	25164.5
65	21993.0
66	17918.5
67	13844.0
68	14699.5
69	15555.0
70	11786.0
71	8017.0
72	6534.0
73	5051.0
74	4004.0
75	2957.0
76	2064.5
77	1172.0
78	807.0
79	442.0
80	466.0
81	490.0
82	402.5
83	315.0
84	200.0
85	85.0
86	44.0
87	3.0
88	1.5
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	423577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.733059160436001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.88643364785214	6.412765565646859
2	9.270623620442914	1.8046305630381252
3	3.308511412423897	0.9660581193029838
4	1.3995682441118684	0.544883220760334
5	0.8586605865088413	0.41786971436126136
6	0.5869939602687559	0.3427948165268653
7	0.5287796832173091	0.36026507577134737
8	0.46086302665728773	0.358848568265038
9	0.41720231886870257	0.365458936627815
>10	12.535474325078225	33.33301855388749
>50	3.141145365900987	20.693049905920294
>100	1.5256991777233366	26.917892142396777
>500	0.05578868217430325	3.503023063103049
>1k	0.024255948771436196	3.9794417543917633
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACCTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	4436	1.047271216331387	No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1990	0.46980832292593794	No Hit
AACCTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	1942	0.458476262875463	No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1423	0.33594836357970337	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1390	0.32815757229500186	No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1272	0.30029959133758444	No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1227	0.2896757850402642	No Hit
AACCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1138	0.2686642570300087	No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1033	0.2438753756695949	No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1005	0.23726500730681788	No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	899	0.21224004136201918	No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	876	0.20681009592116664	No Hit
AACCTAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC	798	0.18839549833914493	No Hit
AACCTAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCAC	752	0.17753560745743985	No Hit
AACCTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	742	0.1751747616135909	No Hit
AACCTAGGGGGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGGCGCC	700	0.1652592090694254	No Hit
AACCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	698	0.16478703990065563	No Hit
AACCTAGGGGGCATGATTCAGACTGGATACTCTCAGTGCCAGCCAAATTACC	688	0.16242619405680667	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	657	0.155107571940875	No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	656	0.1548714873564901	No Hit
AACCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	650	0.15345497985018072	No Hit
AACCTAGGGGCCCTAGCGACCTGTGCCGGGATCTTCGGAGCCGCAACCGACC	645	0.15227455692825625	No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	633	0.14944154191563755	No Hit
AACCTAGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGAT	594	0.1402342431246267	No Hit
AACCTAGGGAAAGATGATCGAGAGGGTCGATTCGTCCTTAAGAACGAGAATG	583	0.13763731269639287	No Hit
AACCTAGGGGCTGGGCAGTGGTGGCGCACGCCTTTAATCCCAGTACATGGGA	569	0.13433212851500434	No Hit
AACCTAGGGGTCCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGACCTG	552	0.13031869058046117	No Hit
AACCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	547	0.1291382676585367	No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	539	0.12724959098345756	No Hit
AACCTAGGGGAGCGCGGCTAGAATCTGCCAACGAGTCGCGTAGTAGCCCTAT	524	0.12370832221768416	No Hit
AACCTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	519	0.12252789929575968	No Hit
AACCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	511	0.12063922262068054	No Hit
AACCTAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	506	0.11945879969875606	No Hit
AACCTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	497	0.11733403843929202	No Hit
AACCTAGGGGAGCTCTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCA	497	0.11733403843929202	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCT	486	0.11473710801105821	No Hit
AACCTAGGGATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCC	473	0.1116680084140546	No Hit
AACCTAGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	471	0.1111958392452848	No Hit
AACCTAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCGG	469	0.11072367007651501	No Hit
AACCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	469	0.11072367007651501	No Hit
AACCTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	464	0.10954324715459056	No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	451	0.10647414755758694	No Hit
AACCTAGGGAAGAGCTGCGTCTAGAGACTCCGCTGGGTCAGGCTGAGGGTGC	434	0.10246070962304375	No Hit
AACCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA	427	0.1008081175323495	No Hit
AACCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	427	0.1008081175323495	No Hit
AACCTAGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCG	426	0.10057203294796459	No Hit
AACCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	425	0.10033594836357972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.028094065541802316	0.0	0.0	0.0	0.0
7	0.028566234710572104	0.0	0.0	0.0	0.0
8	0.028566234710572104	0.0	0.0	0.0	0.0
9	0.028802319294956996	0.0	0.0	0.0	0.0
10	0.037537448917198055	0.0	0.0	0.0	0.0
11	0.05429945440852549	0.0	0.0	0.0	0.0
12	0.0644510915370759	0.0	0.0	0.0	0.0
13	0.07011712156231334	0.0	0.0	0.0	0.0
14	0.08050484327524865	0.0	0.0	0.0	0.0
15	0.08853171914433504	0.0	0.0	0.0	0.0
16	0.09655859501342141	0.0	0.0	0.0	0.0
17	0.10222462503865885	0.0	0.0	0.0	0.0
18	0.10718240131074161	0.0	0.0	0.0	0.0
19	0.11638970010175245	0.0	0.0	0.0	0.0
20	0.12347223763329926	0.0	0.0	0.0	0.0
21	0.12984652141169137	0.0	0.0	0.0	0.0
22	0.1362208051900835	0.0	0.0	0.0	0.0
23	0.14259508896847561	0.0	0.0	0.0	0.0
24	0.14944154191563752	0.0	0.0	0.0	0.0
25	0.15581582569402966	0.0	0.0	0.0	0.0
26	0.16313444780996136	0.0	0.0	0.0	0.0
27	0.17068915451027794	0.0	0.0	0.0	0.0
28	0.18272946831390752	0.0	0.0	0.0	0.0
29	0.1898120058454543	0.0	0.0	0.0	0.0
30	0.19854713546769537	0.0	0.0	0.0	0.0
31	0.20586575758362707	0.0	0.0	0.0	0.0
32	0.21601739471217749	0.0	0.0	0.0	0.0
33	0.22097517098426023	0.0	0.0	0.0	0.0
34	0.22711337017826747	0.0	0.0	0.0	0.0
35	0.24552796776028915	0.0	0.0	0.0	0.0
36	0.2575682815639187	0.0	0.0	0.0	0.0
37	0.2648869036798504	0.0	0.0	0.0	0.0
38	0.2726776949645519	0.0	0.0	0.0	0.0
39	0.279051978742944	0.0	0.0	0.0	0.0
40	0.28920361587149446	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATATC	20	6.3069176E-4	46.000004	17
ATAGGAC	20	6.3069176E-4	46.000004	31
CGCATTG	20	6.3069176E-4	46.000004	18
TAACGGC	40	5.5970304E-9	46.000004	35
ATACTAA	20	6.3069176E-4	46.000004	37
AACGGTT	20	6.3069176E-4	46.000004	46
TGGTTAC	20	6.3069176E-4	46.000004	11
ACTAGCA	20	6.3069176E-4	46.000004	41
GTTCGAT	20	6.3069176E-4	46.000004	13
CGAATTA	20	6.3069176E-4	46.000004	26
CATACGA	20	6.3069176E-4	46.000004	17
CATACCG	20	6.3069176E-4	46.000004	27
TCCGTAT	20	6.3069176E-4	46.000004	23
TTAGTCT	20	6.3069176E-4	46.000004	20
TTAGTAC	20	6.3069176E-4	46.000004	20
TTCGGGA	20	6.3069176E-4	46.000004	11
TAAGGTA	40	5.5970304E-9	46.000004	34
ATACGAT	20	6.3069176E-4	46.000004	18
AATACTA	20	6.3069176E-4	46.000004	36
TCGAATT	20	6.3069176E-4	46.000004	42
>>END_MODULE