FastQCFastQC Report
Fri 17 Jun 2016
SRR1527400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511891
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG54831.0711264702837127No Hit
AACCTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG24440.47744539364825717No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA23480.4586914010990621No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG16880.32975770232334617No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT15700.3067059198149606No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG14510.28345878321752094No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14260.27857493099116803No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA12850.2510300044345378No Hit
AACCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC12230.2389180509131827No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG11230.21938264200777122No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC10970.2143034356923642No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC10950.21391272751425597No Hit
AACCTAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC10460.20434037715060432No Hit
AACCTAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCAC8920.17425584743627062No Hit
AACCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC8690.16976270338802596No Hit
AACCTAGGGGGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGGCGCC8550.16702774614126836No Hit
AACCTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8290.16194853982586138No Hit
AACCTAGGGGGCATGATTCAGACTGGATACTCTCAGTGCCAGCCAAATTACC8150.15921358257910376No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC8130.15882287440099555No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT8110.1584321662228873No Hit
AACCTAGGGGCCCTAGCGACCTGTGCCGGGATCTTCGGAGCCGCAACCGACC7830.15296225172937206No Hit
AACCTAGGGAAAGATGATCGAGAGGGTCGATTCGTCCTTAAGAACGAGAATG7020.13713857051598877No Hit
AACCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT6960.13596644598166407No Hit
AACCTAGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGAT6910.1349896755363935No Hit
AACCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG6900.13479432144733938No Hit
AACCTAGGGATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCC6820.13323148873490645No Hit
AACCTAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG6360.12424520063841717No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG6340.12385449246030893No Hit
AACCTAGGGGTCCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGACCTG6330.12365913837125483No Hit
AACCTAGGGGAGCTCTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCA6310.1232684301931466No Hit
AACCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA6100.11916599432301017No Hit
AACCTAGGGGCTGGGCAGTGGTGGCGCACGCCTTTAATCCCAGTACATGGGA5900.11525891254192788No Hit
AACCTAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCGG5830.11389143391854907No Hit
AACCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC5660.1105704144046291No Hit
AACCTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC5640.11017970622652089No Hit
AACCTAGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT5600.10939828987030441No Hit
AACCTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG5550.10842151942503384No Hit
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACCTAGGGAAG5490.10724939489070914No Hit
AACCTAGGGGAGCGCGGCTAGAATCTGCCAACGAGTCGCGTAGTAGCCCTAT5480.10705404080165505No Hit
AACCTAGGGAGTGCAATATGCCCTCCCCGTGGGGTCCCTTGGCACGGTACTG5430.10607727035638448No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCT5410.10568656217827624No Hit
AACCTAGGGGTGAATATCTCCCTCACAAGCCTCATAGCCAAGATCCTCGAGT5380.10510049991111388No Hit
AACCTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5360.10470979173300567No Hit
AACCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5360.10470979173300567No Hit
AACCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA5240.10236554266435628No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC5150.10060735586286924No Hit
AACCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT5130.100216647684761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT206.3081586E-446.039
TGATCGA301.8593655E-646.015
CTTCGTT206.3081586E-446.031
AGGTATC301.8593655E-646.022
CGGCGTA253.4144814E-546.038
AGCCGAT501.6370905E-1146.021
ATCTCGT600.046.041
TCGTTAT206.3081586E-446.038
ACCGGAT301.8593655E-646.038
CATATCG301.8593655E-646.025
ATGATCG301.8593655E-646.014
GTATTCT253.4144814E-546.037
CGTATAT253.4144814E-546.041
GGTCGAC253.4144814E-546.09
CAACGGT206.3081586E-446.037
ACGCATT301.8593655E-646.042
GACGTAA206.3081586E-446.010
CGCATCA206.3081586E-446.045
GTCTCGC206.3081586E-446.012
TTCGTCA206.3081586E-446.014
Produced by FastQC (version 0.11.5)