FastQCFastQC Report
Fri 17 Jun 2016
SRR1527396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423510
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC46501.0979669901537152No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC18840.4448537224622795No Hit
CCATCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG18020.4254917239262355No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG14370.339307218247503No Hit
CCATCTGGGGCTCCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAG14140.33387641378007604No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC13410.31663951264432955No Hit
CCATCTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA13220.3121531959103681No Hit
CCATCTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG10750.2538310783688697No Hit
CCATCTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA10410.2458029326344124No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC9890.2335245920993601No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG8690.20518996009539328No Hit
CCATCTGGGACTCAGAGCTGTTTGCTTGGGGTTTTTTAAACGGATATTAA8290.19574508276073765No Hit
CCATCTGGGCAGAGTGCAGTGCTCCATCTGGGGGGTGAAGGGGCTGGAGG8120.19173100989350902No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA7840.18511959575925008No Hit
CCATCTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA7680.1813416448253878No Hit
CCATCTGGGGAGGCTGGGAGCCGTGCTAAAGTTCCTGCGAATGCCCCCGA7170.16929942622370192No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT7080.1671743288234044No Hit
CCATCTGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT6950.1641047436896413No Hit
CCATCTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA6900.16292413402280936No Hit
CCATCTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC6840.16150740242261105No Hit
CCATCTGGGCAGGGCCCTGTACGTCTGGGACCCCTGGAGAGTTCTGCCTG6830.16127128048924463No Hit
CCATCTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA6800.16056291468914546No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA6640.15678496375528322No Hit
CCATCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG6600.15584047602181766No Hit
CCATCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT6560.1548959882883521No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCATCTGGGA6390.15088191542112347No Hit
CCATCTGGGGCAGGGCATGGTGGCACACGCCTTTGATCCCAAAACCCAGG6380.15064579348775708No Hit
CCATCTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT6330.1494651838209251No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT6140.1449788670869637No Hit
CCATCTGGGTCTAGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACT6100.14403437935349814No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT6090.14379825742013175No Hit
CCATCTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC6070.143326013553399No Hit
CCATCTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT6050.1428537696866662No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA5940.1402564284196359No Hit
CCATCTGGGGCTTAGAACTGGGCAAACAGCTTGGGGTGAGGCACTTCCGT5890.13907581875280395No Hit
CCATCTGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCC5750.1357701116856745No Hit
CCATCTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC5720.1350617458855753No Hit
CCATCTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG5530.1305754291516139No Hit
CCATCTGGGGGCGGCTTAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGA5500.12986706335151474No Hit
CCATCTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC5450.12868645368468276No Hit
CCATCTGGGGCCGCGTGGACGCCCGCCGGTGAAAGGAGGAGTACCTCTGC5350.12632523435101886No Hit
CCATCTGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC5340.12608911241765247No Hit
CCATCTGGGGACTTCCCGGTCGCGGGTGAAGTGCTGCGTCTCCCCGCCCT5290.12490850275082052No Hit
CCATCTGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCG5130.12113055181695827No Hit
CCATCTGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG5080.11994994215012633No Hit
CCATCTGGGGGAGGCGCAATGGCAGGTGTTGGGGTCGGACCGCTGCAGGG5080.11994994215012633No Hit
CCATCTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC4970.11735260088309603No Hit
CCATCTGGGAGGACCAGACGCCCAGGTCGCCCGCATCCAGTGCCGCAGGA4970.11735260088309603No Hit
CCATCTGGGGTTTGGGATTTGACAAGACGTCCAGGCTCAGTGTGTGGCTG4930.11640811314963047No Hit
CCATCTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA4870.11499138154943211No Hit
CCATCTGGGGCTTTTGCTACCCGGAGGACGAAGGTGCACAGACTTTGCCG4840.11428301574933296No Hit
CCATCTGGGTCCCAGGCTCCTTTGCGGGTAAACAGACATGGCCGGCGAGG4820.11381077188260018No Hit
CCATCTGGGGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTT4810.11357464994923379No Hit
CCATCTGGGCACTTTGGGGAGAGGGGGACATTAGGGCAGCCTCTGAACTG4770.11263016221576823No Hit
CCATCTGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGA4770.11263016221576823No Hit
CCATCTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC4750.11215791834903543No Hit
CCATCTGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTG4710.11121343061556987No Hit
CCATCTGGGGCTCCATCTGGGGAGTGCGTTGCTCCATCTGGGGAGTGCAG4670.11026894288210431No Hit
CCATCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC4600.10861608934853957No Hit
CCATCTGGGGCTCCGCTTCAAGGGAGGCTCTCAGCGATACGTGCCCGGCC4510.10649099194824208No Hit
CCATCTGGGGCTTACACAGTATGGCCGGCGACATTAGCTAGCGCTCGCTC4500.10625487001487569No Hit
CCATCTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC4500.10625487001487569No Hit
CCATCTGGGTCCGGAAGTGGCTGCGCTTGCAGTTTCCCCCGGTCTCTTCA4480.10578262614814289No Hit
CCATCTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG4450.10507426034804374No Hit
CCATCTGGGTACCCACCCTACCCGTTAGGACCCGAAAGATGGTGAACTAT4380.103421406814479No Hit
CCATCTGGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCC4350.10271304101437982No Hit
CCATCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG4330.10224079714764704No Hit
CCATCTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA4320.10200467521428065No Hit
CCATCTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA4310.10176855328091426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGTA302.523915E-644.00000431
CAACCGA302.523915E-644.00000433
GTAATTG302.523915E-644.00000443
TGCATTA302.523915E-644.00000425
TACATAT302.523915E-644.00000436
CCCGTTA600.044.00000421
CTAGTAC302.523915E-644.00000437
AAGTTCT302.523915E-644.00000422
CTAAAGA302.523915E-644.00000412
CGCTATC302.523915E-644.00000425
CGCTAGT302.523915E-644.00000435
TAACAAC302.523915E-644.00000430
CCCCCTA302.523915E-644.00000413
CTACATA302.523915E-644.00000435
CGTCGTA650.044.00000441
ACCGTTG302.523915E-644.00000436
TAAATTC302.523915E-644.00000429
ATAAATC302.523915E-644.00000444
AATAGAC302.523915E-644.00000425
AGTACGG302.523915E-644.00000439
Produced by FastQC (version 0.11.5)