FastQCFastQC Report
Fri 17 Jun 2016
SRR1527395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336235
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA45121.3419185985991942No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC15090.44879325471768255No Hit
CCATCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA14430.42916412628072625No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT11690.34767350216366527No Hit
CCATCTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG11600.3449968028313531No Hit
CCATCTGGGGCTCCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG11180.3325055392805627No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC10980.32655731854209114No Hit
CCATCTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG10090.30008773625589247No Hit
CCATCTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG8950.2661828780466043No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT8020.23852365161271136No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC7020.20878254792035333No Hit
CCATCTGGGACTCAGAGCTGTTTGCTTGGGGTTTTTTAAACGGATATTAACA7020.20878254792035333No Hit
CCATCTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC6680.19867057266495158No Hit
CCATCTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG6630.19718351748033372No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC6320.18796377533570272No Hit
CCATCTGGGCAGAGTGCAGTGCTCCATCTGGGGGGTGAAGGGGCTGGAGGCG6250.18588189807723765No Hit
CCATCTGGGGCAGGGCATGGTGGCACACGCCTTTGATCCCAAAACCCAGGAG6240.18558448704031405No Hit
CCATCTGGGCAGGGCCCTGTACGTCTGGGACCCCTGGAGAGTTCTGCCTGCT6230.18528707600339048No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA6110.18171814356030752No Hit
CCATCTGGGGAGGCTGGGAGCCGTGCTAAAGTTCCTGCGAATGCCCCCGAGA5940.17666215593260665No Hit
CCATCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC5470.1626838371971984No Hit
CCATCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG5470.1626838371971984No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT5420.1611967820125805No Hit
CCATCTGGGTCTAGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCG5390.16030454890180976No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC5370.15970972682796258No Hit
CCATCTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT5270.15673561645872677No Hit
CCATCTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC5160.15346409505256742No Hit
CCATCTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA5120.15227445090487307No Hit
CCATCTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT5080.15108480675717875No Hit
CCATCTGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATCT5030.14959775157256086No Hit
CCATCTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG4910.1460288191294779No Hit
CCATCTGGGGCTTAGAACTGGGCAAACAGCTTGGGGTGAGGCACTTCCGTGT4780.14216247564947135No Hit
CCATCTGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCT4580.13621425491099975No Hit
CCATCTGGGGCCGCGTGGACGCCCGCCGGTGAAAGGAGGAGTACCTCTGCCG4550.135322021800229No Hit
CCATCTGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTG4520.13442978868945826No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCATCTGGGAAG4520.13442978868945826No Hit
CCATCTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT4510.1341323776525347No Hit
CCATCTGGGGCTTTTGCTACCCGGAGGACGAAGGTGCACAGACTTTGCCGTC4450.1323479114309932No Hit
CCATCTGGGGACTTCCCGGTCGCGGGTGAAGTGCTGCGTCTCCCCGCCCTCT4430.13175308935714605No Hit
CCATCTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT4420.13145567832022245No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG4400.1308608562463753No Hit
CCATCTGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCT4340.12907639002483381No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC4230.12580486861867446No Hit
CCATCTGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC4230.12580486861867446No Hit
CCATCTGGGGGAGGCGCAATGGCAGGTGTTGGGGTCGGACCGCTGCAGGGGA4200.12491263550790371No Hit
CCATCTGGGTCCCAGGCTCCTTTGCGGGTAAACAGACATGGCCGGCGAGGGA4190.12461522447098011No Hit
CCATCTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC4180.12431781343405653No Hit
CCATCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG4140.12312816928636222No Hit
CCATCTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG4100.1219385251386679No Hit
CCATCTGGGGGCGGCTTAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGC4070.12104629202789716No Hit
CCATCTGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG4060.12074888099097357No Hit
CCATCTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA3990.11866700373250852No Hit
CCATCTGGGGTTTGGGATTTGACAAGACGTCCAGGCTCAGTGTGTGGCTGTG3830.11390842714173123No Hit
CCATCTGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT3810.11331360506788407No Hit
CCATCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA3800.11301619403096048No Hit
CCATCTGGGGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCC3800.11301619403096048No Hit
CCATCTGGGGCTTACACAGTATGGCCGGCGACATTAGCTAGCGCTCGCTCAA3790.1127187829940369No Hit
CCATCTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG3790.1127187829940369No Hit
CCATCTGGGTCCGGAAGTGGCTGCGCTTGCAGTTTCCCCCGGTCTCTTCAGG3730.11093431677249542No Hit
CCATCTGGGGTACCCTGCTGGTGACTTTGCAGCCCCGCCTTGCCTGACCTCC3730.11093431677249542No Hit
CCATCTGGGGGGTCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTG3670.10914985055095394No Hit
CCATCTGGGCACTTTGGGGAGAGGGGGACATTAGGGCAGCCTCTGAACTGGG3630.10796020640325964No Hit
CCATCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA3630.10796020640325964No Hit
CCATCTGGGAGGACCAGACGCCCAGGTCGCCCGCATCCAGTGCCGCAGGACA3620.10766279536633605No Hit
CCATCTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT3590.1067705622555653No Hit
CCATCTGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGG3560.10587832914479456No Hit
CCATCTGGGGAAGCTTTGGGGAAGGCGCTGGCGGAGTACACAGACGCCGGGG3470.10320162981248233No Hit
CCATCTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT3470.10320162981248233No Hit
CCATCTGGGGCTCCGCTTCAAGGGAGGCTCTCAGCGATACGTGCCCGGCCCA3450.10260680773863519No Hit
CCATCTGGGGCTCCATCTGGGGAGTGCGTTGCTCCATCTGGGGAGTGCAGTG3440.1023093967017116No Hit
CCATCTGGGGAGGCTCCTTTGCGGGTAAACAGACATGGCCGGCGAGGGAGAT3430.10201198566478802No Hit
CCATCTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA3420.10171457462786444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA253.411964E-546.040
TATCATG206.305053E-446.011
GTGCATA405.5915734E-946.017
CTTAACG206.305053E-446.032
GTTTGCG206.305053E-446.031
CTTCGAA206.305053E-446.031
GTGCACT206.305053E-446.013
TAATATT206.305053E-446.041
CAGGTAT206.305053E-446.036
GAAATGA206.305053E-446.09
ATGGGAC206.305053E-446.033
TAGAGCT253.411964E-546.033
AGGTACA253.411964E-546.046
GTACCTT206.305053E-446.011
TGATACT206.305053E-446.039
CTCACGT405.5915734E-946.046
CCTTAAC206.305053E-446.043
GGTACGA501.6370905E-1146.022
CCCCTAA206.305053E-446.014
ATCTATT206.305053E-446.010
Produced by FastQC (version 0.11.5)