FastQCFastQC Report
Fri 17 Jun 2016
SRR1527394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475900
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA56401.1851229249842403No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC21360.4488337886110527No Hit
CCATCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA20030.42088674091195627No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT16550.3477621349022904No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC15880.3336835469636478No Hit
CCATCTGGGGCTCCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG15100.31729354906492957No Hit
CCATCTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG14750.3099390628283253No Hit
CCATCTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG12190.25614624921201934No Hit
CCATCTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG12010.2523639420046228No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT11210.23555368774952723No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC10950.23009035511662115No Hit
CCATCTGGGACTCAGAGCTGTTTGCTTGGGGTTTTTTAAACGGATATTAACA9770.2052952300903551No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC9630.2023534355957134No Hit
CCATCTGGGGCAGGGCATGGTGGCACACGCCTTTGATCCCAAAACCCAGGAG9260.19457869300273165No Hit
CCATCTGGGCAGAGTGCAGTGCTCCATCTGGGGGGTGAAGGGGCTGGAGGCG8870.18638369405337255No Hit
CCATCTGGGCAGGGCCCTGTACGTCTGGGACCCCTGGAGAGTTCTGCCTGCT8820.1853330531624291No Hit
CCATCTGGGGAGGCTGGGAGCCGTGCTAAAGTTCCTGCGAATGCCCCCGAGA8520.17902920781676823No Hit
CCATCTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG8480.17818869510401344No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT8140.17104433704559782No Hit
CCATCTGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATCT8140.17104433704559782No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCATCTGGGAAG8100.17020382433284303No Hit
CCATCTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC7740.16263920991805No Hit
CCATCTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA7730.16242908173986131No Hit
CCATCTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT7620.16011767177978567No Hit
CCATCTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC7600.1596974154234083No Hit
CCATCTGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCT7600.1596974154234083No Hit
CCATCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC7580.15927715906703088No Hit
CCATCTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG7420.15591510821601176No Hit
CCATCTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT7400.15549485185963438No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG7360.1546543391468796No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA7330.1540239546123135No Hit
CCATCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG7150.150241647404917No Hit
CCATCTGGGTCTAGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCG7000.14708972473208656No Hit
CCATCTGGGGCTTAGAACTGGGCAAACAGCTTGGGGTGAGGCACTTCCGTGT6860.14414793023744485No Hit
CCATCTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC6740.14162639209918051No Hit
CCATCTGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTG6450.13553267493170834No Hit
CCATCTGGGGCCGCGTGGACGCCCGCCGGTGAAAGGAGGAGTACCTCTGCCG6390.13427190586257617No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC6350.13343139314982141No Hit
CCATCTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG6300.1323807522588779No Hit
CCATCTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT6280.13196049590250053No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC6150.1292288295860475No Hit
CCATCTGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC6070.12754780416053793No Hit
CCATCTGGGGGCGGCTTAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGC6050.12712754780416052No Hit
CCATCTGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCT5960.1252363942004623No Hit
CCATCTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT5790.12166421517125446No Hit
CCATCTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT5680.1193528052111788No Hit
CCATCTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA5630.11830216432023534No Hit
CCATCTGGGAGGACCAGACGCCCAGGTCGCCCGCATCCAGTGCCGCAGGACA5600.11767177978566927No Hit
CCATCTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA5590.11746165160748055No Hit
CCATCTGGGCACTTTGGGGAGAGGGGGACATTAGGGCAGCCTCTGAACTGGG5580.11725152342929186No Hit
CCATCTGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG5560.11683126707291448No Hit
CCATCTGGGGCTTTTGCTACCCGGAGGACGAAGGTGCACAGACTTTGCCGTC5560.11683126707291448No Hit
CCATCTGGGGGAGGCGCAATGGCAGGTGTTGGGGTCGGACCGCTGCAGGGGA5560.11683126707291448No Hit
CCATCTGGGTCCCAGGCTCCTTTGCGGGTAAACAGACATGGCCGGCGAGGGA5540.11641101071653709No Hit
CCATCTGGGGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCC5490.11536036982559361No Hit
CCATCTGGGGTTTGGGATTTGACAAGACGTCCAGGCTCAGTGTGTGGCTGTG5430.11409960075646143No Hit
CCATCTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA5370.11283883168732928No Hit
CCATCTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT5330.11199831897457449No Hit
CCATCTGGGGTACCCTGCTGGTGACTTTGCAGCCCCGCCTTGCCTGACCTCC5210.10947678083631014No Hit
CCATCTGGGGCTTACACAGTATGGCCGGCGACATTAGCTAGCGCTCGCTCAA5180.10884639630174406No Hit
CCATCTGGGGAGGCTCCTTTGCGGGTAAACAGACATGGCCGGCGAGGGAGAT5130.10779575541080058No Hit
CCATCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA5100.1071653708762345No Hit
CCATCTGGGGCTCCGCTTCAAGGGAGGCTCTCAGCGATACGTGCCCGGCCCA5060.10632485816347974No Hit
CCATCTGGGTACCCACCCTACCCGTTAGGACCCGAAAGATGGTGAACTATGC5020.10548434545072495No Hit
CCATCTGGGTCATGCCGTGCATAACGGTCTGACTGTCCTGCCGCAAACCGAG5000.10506408909434756No Hit
CCATCTGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGG4990.10485396091615887No Hit
CCATCTGGGTCCGGAAGTGGCTGCGCTTGCAGTTTCCCCCGGTCTCTTCAGG4980.10464383273797018No Hit
CCATCTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT4930.10359319184702667No Hit
CCATCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG4880.10254255095608321No Hit
CCATCTGGGGCTCCATCTGGGGAGTGCGTTGCTCCATCTGGGGAGTGCAGTG4820.10128178188695104No Hit
CCATCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA4800.10086152553057365No Hit
CCATCTGGGGAAGCTTTGGGGAAGGCGCTGGCGGAGTACACAGACGCCGGGG4790.10065139735238496No Hit
CCATCTGGGACAGAAAGTTGTGGTCTGATCAGTTAATTTGTATGTGGCAATG4770.10023114099600758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC351.0178883E-746.00000419
CAAACCG351.0178883E-746.00000444
CGAAACG351.0178883E-746.00000437
GACGTTG351.0178883E-746.00000422
CTATATC351.0178883E-746.00000436
TTAGACT351.0178883E-746.00000413
CGTTGAC351.0178883E-746.00000424
GAATATA351.0178883E-746.00000440
TAACGGT351.0178883E-746.00000422
ACGATCT351.0178883E-746.00000441
ACTAGAT351.0178883E-746.00000418
ACTAATT351.0178883E-746.00000444
AGCGAAT351.0178883E-746.00000412
ATTGGTA351.0178883E-746.00000446
AATTATC351.0178883E-746.00000445
CATAACG351.0178883E-746.00000420
TACTACA351.0178883E-746.00000432
ATAACGG351.0178883E-746.00000421
GTGCGTT700.046.00000424
ACGCGAC206.307709E-446.027
Produced by FastQC (version 0.11.5)