FastQCFastQC Report
Fri 17 Jun 2016
SRR1527393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88527
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12151.3724626385170626No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9111.0290645791679374No Hit
CGTGGCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC4210.4755611282433608No Hit
CGTGGCGGGACGGAGAAACACCTGATTTCCAGGAAAATCCCCTCAGATGG4090.4620059416901058No Hit
CGTGGCGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGG3840.43376596970415804No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3740.422469980909779No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.41682198651258934No Hit
CGTGGCGGCACAGAAGATTCAGGCAAGCCCACAGGAGGTAATTCTGGCAA3470.39197081116495536No Hit
CGTGGCGGCAACACTGTAGGTTCCAAAGGAAATCAGAAAGTGAAGGCAAA3410.38519321788832783No Hit
CGTGGCGGAATCGAGGACCCGTAAGAAGGCTGACAGAGCCCCTGTGGCCA3200.3614716414201317No Hit
CGTGGCGGTAGCAAGTAATGGATGCCACCTGCCCCAGCATTATGGGGGTC3190.3603420425406938No Hit
CGTGGCGGCAGGGAGATGTGAAATCACTGCCTCTTGTAGCCACTGGGGAC3150.35582364702294217No Hit
CGTGGCGGAAGGTCTTGGGGTCTCAAGGTGAGTCAGTTTGCTGTCCTGTG3040.3433980593491251No Hit
CGTGGCGGGAGCAAGGGCCAGGTCATGGTGGCACACACCTTTGGTCCCAG2790.31515808736317735No Hit
CGTGGCGGAGTTGGGAGCTGTCTCCTGTGCCTCCCCTCCCTATCCCCAGT2750.3106396918454257No Hit
CGTGGCGAGAGAAGTCCTGGGTTCAAATCCCAGCAACCACATGGTGAGTC2600.293695708653857No Hit
CGTGGCGGTATGCAAACTAGCAAATACTTATTAATAAGAAAGTAGCAATC2480.2801405221006021No Hit
CGTGGCGGGCAGCGCTGAGGGACCAGTCTTTCCTGTGACTGCCACCAGCG2440.2756221265828504No Hit
CGTGGCGGGGCCTAAGAAATACTACAGGGGTCATCCTTTTATAAGGACAA2420.2733629288239746No Hit
CGTGGCGGGATGTAATCAGTCACCTGGGTGGTGGTGGTGCATGCCTTTAT2400.2711037310650988No Hit
CGTGGCGGCGTCTTTCTCCCCGGATAGAAAAGGGGGACCTCAGCATATGA2310.2609373411501576No Hit
CGTGGCGGCAAGACCAATCCTGTAGTGAAAGTCGGATTCAGTGACCTCTT2260.25528934675296805No Hit
CGTGGCGGAGATATGGGATGCACATGCACAGGTGCATTGTGGGTAGAGTG2260.25528934675296805No Hit
CGTGGCGGGATATAAATGAATGAAATATAGATAAACTGTGAGATAAATAT2250.25415974787353013No Hit
CGTGGCGGGCTGTATGATATATGAAGAAGAGGAGGAGGAGGAGAGAAGAA2210.24964135235577847No Hit
CGTGGCGAGGGATGGACAGTTTAGAGGTATAGTCCTCTGCTGTGTTGGAC2200.24851175347634058No Hit
CGTGGCGAGAGAGAGACTCCTGCTGGTATCAGACTCTCAGGATCTGCCAG2190.24738215459690263No Hit
CGTGGCGGGGCGAAAGTCAGCGTACTGTTCAACCTCCCGGCAGTCGGTTT2180.24625255571746474No Hit
CGTGGCGGAGTCTATAAAACTTTAAATTAATTCTGTCTTGAAACACAGGA2160.2439933579585889No Hit
CGTGGCGGCACCTTTATTAAGAAATTATACTAAACAATGGCGTCCTTGAT2150.24286375907915098No Hit
CGTGGCGGGGTTTAGGATGGAGGCCTTTCTGTTGAGTGGAAATGCACCTT2070.23382696804364772No Hit
CGTGGCGGGCTGTGCGGTGCTGTGCTGTGCTGTGCTGTGCAGCTAATCAG2070.23382696804364772No Hit
CGTGGCGGACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAAACCATT2020.22817897364645814No Hit
CGTGGCGGTCATCTACATCGAACGAGTCCAGCGAGAGAAGGCTAATGGCA1980.2236605781287065No Hit
CGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC1960.22140138036983065No Hit
CGTGGCGGAGCTCGCTCTCAGGAACGCCAGGGCTCCCGAGGGCGGTGCCA1950.22027178149039275No Hit
CGTGGCGGCGGGCAGTGGTGGTGCACACCTTTAGTCTCAGCACTTGGGAG1920.21688298485207902No Hit
CGTGGCGGGGCCTAGAACATATGTAGTATTCAGACAGTCATCAATGAAGG1910.21575338597264113No Hit
CGTGGCGGACTTATGTCAATGAACAGCTTGCAAGTCCTGTGACACAAGAA1860.21010539157545155No Hit
CGTGGCGGCCCGCACGGGTGAACCAGTGGCACAGGTACACCAGCATCGCC1860.21010539157545155No Hit
CGTGGCGGGCAATGGGAGAAATCATGAATCGACTTCTGCCTACTGGTCCT1850.20897579269601366No Hit
CGTGGCGGATTTGAAGATTTGTTTGGTTTTAGTGATTTATTGCCTCAAAA1780.20106860053994827No Hit
CGTGGCGGAGTATAAGGATGAAAAAGAAAGCCTTGTTAAGGTTATAAAAG1780.20106860053994827No Hit
CGTGGCGGCAGCGTTACAGTTTGGGTTTCATTGCTGTGGTATAAGAACAG1700.19203180950444498No Hit
CGTGGCGGAACTGAGTGAGTTCCAGGACAGCCAGGGCTACACTGAGAAAC1660.18751341398669333No Hit
CGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCC1620.18299501846894167No Hit
CGTGGCGGGCAATCTCCCAGGGCTCCCTCCCACCAGCACCTGTGGTGGCT1570.17734702407175212No Hit
CGTGGCAGGATAAATGGATTTTGAAATGTCTGACCTCACCTGTTAAGTCC1570.17734702407175212No Hit
CGTGGCGGGATCTAGCTTCGTGCTCAGACACGTCTCAACAAGACCTTAGA1540.1739582274334384No Hit
CGTGGCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.1739582274334384No Hit
CGTGGCGGGACCTAGTTGAGTGGGGAGGGGGGGGCATCTTCTGACAGCCT1530.17282862855400047No Hit
CGTGGCGGACACTTCAGAGTTTATACTCCTGAGATTTAGGGCCTGCCCCA1530.17282862855400047No Hit
CGTGGCGGGCTCTTCCACAACCCTCATGTGAACCCACTTCCGACCGGCTA1500.16943983191568673No Hit
CGTGGCGGGACGAAGACTTCATCTTGCCGGCGCTTGCGTGCTTCCAAGAC1480.16718063415681092No Hit
CGTGGCGGCAAGGAGACCGTAGGCTTCGGAATGCTCAAGGCTAAGGCCCA1480.16718063415681092No Hit
CGTGGCGGCTGTAAATTTATGTGCATGGTGTACGTGCAGATACATGAGAA1440.1626622386390593No Hit
CGTGGCGGAGATGAAAAGGTTATAGCATCCTTAACTGAGGCTGTAGGCTG1440.1626622386390593No Hit
CGTGGCGGGATCCCATCAGGCCTCTGACCCAGACTGCTGTGTCCCTTCTT1440.1626622386390593No Hit
CGTGGCGAGAGAGAGACAGGCCACCACCAGGGCCCCTGGCCCGCCCACTT1430.16153263975962137No Hit
CGTGGCGGATCATTTCTGACTTTCTCTGTACTTCCCTAAATAAATATCTC1400.1581438431213076No Hit
CGTGGCGGATCAAACCATTCCTTCTGTAGCTCAGGAGAGCGTCCCTACCC1380.1558846453624318No Hit
CGTGGCGGGACGTACATGCACAACACACATATGTACACACACACATGCAC1330.15023665096524225No Hit
CGTGGCGGGATCTTGGCTATCTTTTATCAAAAGTTTTAGCGCCTTCCTTG1320.14910705208580433No Hit
CGTGGCGGGCTCCATGTCTGTGATGTGTCTGGAGTCTCTCGGGACACGAC1290.1457182554474906No Hit
CGTGGCGGGATCTAAAAAAAGGAAAAAAAAGAAAAGAAAGAAAGAAAGAG1270.14345905768861478No Hit
CGTGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.14119985992973896No Hit
CGTGGCGGGATGCAAATTCTGTTTTATTTTATCCCTAATAACAGAAGTCT1250.14119985992973896No Hit
CGTGGCGGGAAGTCAACTATGAAGAATTCGTACAGATGATGACTGCAAAA1220.1378110632914252No Hit
CGTGGCGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGTA1220.1378110632914252No Hit
CGTGGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.1378110632914252No Hit
CGTGGCGGGGCCCTGCCTCTGGTCTAACCCTGTGTACCCTCTGAAGTCAC1210.1366814644119873No Hit
CGTGGCGGGACCTCAGAAAAAAATGCTTCATTGAGTATTCATTAAACCAC1190.13442226665311147No Hit
CGTGGCGCGCACCTTCCAGCATACTAAAATCTGAGGATGCTCAAGTCCCT1160.13103347001479773No Hit
CGTGGCGGGGCGCCCTCACACAGGAATAAAGGCACTATCACTACTTTTCT1130.12764467337648402No Hit
CGTGGCGGTGACCTGTGAACATCAACAAGATACCCACTCCATTTCCTGGG1130.12764467337648402No Hit
CGTGGCGGGATGTGAATGCTGATGCCCTGGAAGACCAGAGATGTCACATC1120.1265150744970461No Hit
CGTGGCGGTACGTGTGACCTGTGAGGACATAATGCCTTGGTTTTTTCTAA1110.12538547561760818No Hit
CGTGGCGGGGGGCACTCCGAGCCGATGCCACCGGCCCGCGGGTCTCTGAA1100.12425587673817029No Hit
CGTGGCGGGACTAAGCAAGAAGGGCAGTTTCCAGAACTATTCAGAGGAAA1100.12425587673817029No Hit
CGTGGCGGGACTAAGATCATGGCTATGCAGGCAGTATAGTGCTTCTGGCT1100.12425587673817029No Hit
CGTGGCGGATGATTGGCCCCATATATCCCATAAGCTGTGAATTGGCTCCT1080.12199667897929445No Hit
CGTGGCCGGGCCCAAACTTTGGAACAGTATGCCATGAGAGCTTTTGCAGA1070.12086708009985654No Hit
CGTGGCGGGATCCAAGGCAACAATTTATCATGATGCTGTAGCATTCAACC1060.11973748122041862No Hit
CGTGGCGGGGTATAAATGAATGAAATATAGATAAACTGTGAGATAAATAT1060.11973748122041862No Hit
CGTGGCGGGATGTCTGTATAACTCTTTGGTCTTGCCCTAATAGTATCATG1040.1174782834615428No Hit
CGTGGCGGGATGGAGAGAGTCTCACACTTCTGGCTCATGAAGGAGCTGTC1040.1174782834615428No Hit
CGTGGCGGGATGAAACTCAACTGTGACTCTCGATCAGCCCAGCCCATGTG1030.11634868458210489No Hit
CGTGGCAGGATGAACTACCCAGTATCCCCCAGAGCTTGTGTCTCTAGCTG1030.11634868458210489No Hit
CGTGGCGGGACCCCTGCACGAGGCAAATCATAGTCAGCTGCTGTGGACAG1020.11521908570266699No Hit
CGTGGCGGTAGGACCAAAGTTCCCAGCTGCAGGGAGCAAGGGAAGGGTGG1010.11408948682322907No Hit
CGTGGCGGCAGTTGGGGATGGGGCAGCTTGCCTATTTATTTTGCTTTAAG1000.11295988794379117No Hit
CGTGGCGGGGTGTTGTGACCCAGTACCCGCTTTGAACTTTCTAACAGAGG1000.11295988794379117No Hit
CGTGGCGGAGTTAAACTCAATAAAAAAGGGTTGTAACCAGCCAGGTGTGA990.11183028906435324No Hit
CGTGGCGGGATGTTCTGTACAGTAGATTTCATTAAGAAAAATTGTGGACA980.11070069018491532No Hit
CGTGGCGGGACCAAGACCTTTTAAAAACCCTTTTATGGCCTCTGTAGGCA980.11070069018491532No Hit
CGTGGCGGGGTGTACCCTGACAGCATCATGTGTGTCAACCTGTGTCAATT980.11070069018491532No Hit
CGTGGCGGCAAGGAGACGTATGGGGACACTGAAGCCATCTGCCGGCGCCT980.11070069018491532No Hit
CGTGGCGGGGTGCAAATTCTGTTTTATTTTATCCCTAATAACAGAAGTCT970.10957109130547743No Hit
CGTGGCGGCACCTATGCGCTTTTTTTTTCTACTAAGCTGAAAAAGTTCCT950.10731189354660159No Hit
CGTGGCGGCATACAGCAAAAGCACATACAATTCCGCATCCCTTGGGAGAG950.10731189354660159No Hit
CGTGGCGGGAAGATCATGATTGCTTAGAGATGTTTTTTATTAAAGATCAC950.10731189354660159No Hit
CGTGGCGGTACCCACAGTGCCACTGCCATCCTGTCCCTTGCTCTGTACCG940.1061822946671637No Hit
CGTGGCGGGGTATACGTCATTATGTCCCTGGTTTTTATAACTATGGTGCG940.1061822946671637No Hit
CGTGGCGGGGCGCCAACATACAACAGGGAGATGTGTCCCAGTCCACTTCC940.1061822946671637No Hit
CGTGGCGGGATGAACAGCACCAAGCTCTCGGCTGACACCTACGAGGATCT930.10505269578772577No Hit
CGTGGCGGGGTGTAATCAGTCACCTGGGTGGTGGTGGTGCATGCCTTTAT920.10392309690828788No Hit
CGTGGCGGCAGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGA900.10166389914941204No Hit
CGTGGCGGGCTCTACCCTGAAAGCTGAGTTTATTTTCTGAGATTTTCCCT900.10166389914941204No Hit
CGTGGCGGGATCCTTGCCTCAGCTTCTGATGTCCCCCTTCCTCCCACACA890.10053430026997413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG2350.044.0000046
TTATGGT302.5012287E-644.08
CGTTTCC207.817111E-444.039
CTACACT207.817111E-444.037
TAGAACA254.409514E-544.013
ACACGTC207.817111E-444.028
TTCACTA254.409514E-544.013
GAGGTAT254.409514E-544.024
GAGGTAA254.409514E-544.035
TTGCATT254.409514E-544.019
ACTGCAA207.817111E-444.042
TGGTCCC302.5012287E-644.042
AACAATT302.5012287E-644.019
GAGAAGT207.817111E-444.09
CCGGATA351.425924E-744.020
TGGTTCT207.817111E-444.024
ACCGGCC351.425924E-744.030
TCCCATA207.817111E-444.026
AACGCCA351.425924E-744.023
GTATGAT207.817111E-444.012
Produced by FastQC (version 0.11.5)