FastQCFastQC Report
Fri 17 Jun 2016
SRR1527392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146624
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8430.5749399825403754No Hit
CGTGGCGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTG8150.5558435181143606No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7910.5394751200349193No Hit
CGTGGCGGGATGTGAATGCTGATGCCCTGGAAGACCAGAGATGTCACATCCC4880.33282409428197296No Hit
CGTGGCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC4140.28235486687036226No Hit
CGTGGCGGGATCTTGGCTATCTTTTATCAAAAGTTTTAGCGCCTTCCTTGAT4020.27417066783064165No Hit
CGTGGCGGGATATAAATGAATGAAATATAGATAAACTGTGAGATAAATATCA3920.26735050196420773No Hit
CGTGGCGGGACCAAAAGCACATCCAGCCAGTGGCCTGAACTTTGAAACCAGC3780.25780226975120035No Hit
CGTGGCGGGAGTTACCATTTTCATTAACAGCATACACCCTTCAATTGTGTTG3630.24757202095154954No Hit
CGTGGCGGGATCCCATCAGGCCTCTGACCCAGACTGCTGTGTCCCTTCTTCC3480.23734177215189875No Hit
CGTGGCGGGAAGTCAACTATGAAGAATTCGTACAGATGATGACTGCAAAATG3460.23597773897861196No Hit
CGTGGCGGGACGGAGAAACACCTGATTTCCAGGAAAATCCCCTCAGATGGGC3420.23324967263203839No Hit
CGTGGCGGGATCTAAAAAAAGGAAAAAAAAGAAAAGAAAGAAAGAAAGAGAG3350.22847555652553472No Hit
CGTGGCGGGGTTTAGGATGGAGGCCTTTCTGTTGAGTGGAAATGCACCTTAA3320.22642950676560453No Hit
CGTGGCGGCAACACTGTAGGTTCCAAAGGAAATCAGAAAGTGAAGGCAAACG3280.223701440419031No Hit
CGTGGCGGACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAAACCATTCG3260.22233740724574422No Hit
CGTGGCGGGGTGTACCCTGACAGCATCATGTGTGTCAACCTGTGTCAATTAT3240.22097337407245743No Hit
CGTGGCGGGATGCAAATTCTGTTTTATTTTATCCCTAATAACAGAAGTCTCC3090.21074312527280661No Hit
CGTGGCGGGACCCAAAGTTGAAAGCAAAGAGAAAGTGTTAGTGAAAAAAGAC3030.2066510257529463No Hit
CGTGGCGGGCTCTTCCACAACCCTCATGTGAACCCACTTCCGACCGGCTATG2930.19983085988651245No Hit
CGTGGCGGCAGGGAGATGTGAAATCACTGCCTCTTGTAGCCACTGGGGACCT2890.19710279353993887No Hit
CGTGGCGGAAGGTCTTGGGGTCTCAAGGTGAGTCAGTTTGCTGTCCTGTGAC2860.19505674378000873No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.19301069402007856No Hit
CGTGGCGGGGTGTTGTGACCCAGTACCCGCTTTGAACTTTCTAACAGAGGCA2770.18891859450021825No Hit
CGTGGCGGCACAGAAGATTCAGGCAAGCCCACAGGAGGTAATTCTGGCAAGC2760.18823657791357484No Hit
CGTGGCGGGAGCAAGGGCCAGGTCATGGTGGCACACACCTTTGGTCCCAGCA2690.18346246180707115No Hit
CGTGGCGGGGCCCTGCCTCTGGTCTAACCCTGTGTACCCTCTGAAGTCACCC2660.18141641204714098No Hit
CGTGGCGGGCGAGAAGGGGCCGAAGGCGAAATGAACACAAAGCCTCGAAGCC2650.18073439546049758No Hit
CGTGGCGGAGTTGGGAGCTGTCTCCTGTGCCTCCCCTCCCTATCCCCAGTCC2650.18073439546049758No Hit
CGTGGCGGGCAGCGCTGAGGGACCAGTCTTTCCTGTGACTGCCACCAGCGAA2630.17937036228721082No Hit
CGTGGCGGTAGCAAGTAATGGATGCCACCTGCCCCAGCATTATGGGGGTCAA2600.17732431252728065No Hit
CGTGGCGGGACCAAGACCTTTTAAAAACCCTTTTATGGCCTCTGTAGGCACC2560.1745962461807071No Hit
CGTGGCGGGATTTGGCTTAATGTAATATGTCACTAATTGTCATATATCCTCA2440.16641204714098648No Hit
CGTGGCGGGGCCTAAGAAATACTACAGGGGTCATCCTTTTATAAGGACAAAA2430.16573003055434307No Hit
CGTGGCGGGCTCCAGCTCCATCAGCTGGCCGTGGCAGAGGCCAAGCCCGCTT2410.1643659973810563No Hit
CGTGGCGGAATCGAGGACCCGTAAGAAGGCTGACAGAGCCCCTGTGGCCAAT2400.1636839807944129No Hit
CGTGGCGAGAGAAGTCCTGGGTTCAAATCCCAGCAACCACATGGTGAGTCAC2380.16231994762112614No Hit
CGTGGCGGGGCGAAAGTCAGCGTACTGTTCAACCTCCCGGCAGTCGGTTTCA2370.16163793103448276No Hit
CGTGGCGGGATCCTTGCCTCAGCTTCTGATGTCCCCCTTCCTCCCACACAGT2370.16163793103448276No Hit
CGTGGCGGGATGAATGAGAAAGGCAAACCAACGATGAGTTGGCTGAGATGGT2310.15754583151462243No Hit
CGTGGCGGGATGTAATCAGTCACCTGGGTGGTGGTGGTGCATGCCTTTATTT2280.15549978175469228No Hit
CGTGGCGGGATCTGGGATACAGTCTAACTCATCTGACTTAATATGCATTTAA2270.15481776516804888No Hit
CGTGGCGGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGTAGG2240.15277171540811874No Hit
CGTGGCGGGGATAAGGACGGCATCACCAACGTACCGCCGGGCACGCGCCAGT2170.14799759930161502No Hit
CGTGGCGGGGGTAAGGTTTTTTGTACTCAATTCTTTAAGAAATGACAAATTT2120.1445875163683981No Hit
CGTGGCGGGGTGCTGGGAAAGTGCTGCCTGCCCTTTCACCAGCCTGAAGCTC2090.14254146660846792No Hit
CGTGGCGGAGTCTATAAAACTTTAAATTAATTCTGTCTTGAAACACAGGAGA2060.14049541684853775No Hit
CGTGGCGGACTTATGTCAATGAACAGCTTGCAAGTCCTGTGACACAAGAACT2050.13981340026189437No Hit
CGTGGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.13844936708860758No Hit
CGTGGCGGCGGGCAGTGGTGGTGCACACCTTTAGTCTCAGCACTTGGGAGGC2020.1377673505019642No Hit
CGTGGCGGGCTGTTGAAGCTGATGGAAGGGCTTTGACGTGTCATGTTGTTCT2000.13640331732867744No Hit
CGTGGCGGATCAAACCATTCCTTCTGTAGCTCAGGAGAGCGTCCCTACCCCA2000.13640331732867744No Hit
CGTGGCGGGCTGATGGAGAAGATACAGGCCTCTGTGGCTACCAACCCCCTCA2000.13640331732867744No Hit
CGTGGCGGGCTGATGGAGAAGATACAGGCCTCTGTGGCTACCAACCCCATCA1980.13503928415539065No Hit
CGTGGCGGTATGCAAACTAGCAAATACTTATTAATAAGAAAGTAGCAATCCC1960.13367525098210387No Hit
CGTGGCGGCACCTTTATTAAGAAATTATACTAAACAATGGCGTCCTTGATTG1940.1323112178088171No Hit
CGTGGCGGGATGGAAGCCAAGCATGCAGTTTCCTTCATGACCTGTACACAGA1940.1323112178088171No Hit
CGTGGCGGGATGAACAGCACCAAGCTCTCGGCTGACACCTACGAGGATCTGA1940.1323112178088171No Hit
CGTGGCGGGACAGTAACTGGTCAAGAGACATAATTATGCTGCTGCTGCTAAA1920.13094718463553034No Hit
CGTGGCGGGCAATCTCCCAGGGCTCCCTCCCACCAGCACCTGTGGTGGCTGC1920.13094718463553034No Hit
CGTGGCGGGCTCTACCCTGAAAGCTGAGTTTATTTTCTGAGATTTTCCCTCT1920.13094718463553034No Hit
CGTGGCGGGATCCGATGATTGTTTTGGTTTCTATTCCCTTTCTAACTGCCCA1900.12958315146224358No Hit
CGTGGCGGGGTCTAGAAGAGTTTGAGCTCCTGGGAAAGGCCTATGGGAAAGC1880.1282191182889568No Hit
CGTGGCGGGAATTAGTGTATGGGGGAAAACGAGTAAGATTGCTTGCCACTTC1860.12685508511567No Hit
CGTGGCGGGGAATGCAGATGAATTTGAAGACATGGTTGCTGAGAAGCGGCTC1860.12685508511567No Hit
CGTGGCGGCTGTAAATTTATGTGCATGGTGTACGTGCAGATACATGAGAAGG1850.12617306852902663No Hit
CGTGGCGGAGATGAAAAGGTTATAGCATCCTTAACTGAGGCTGTAGGCTGCT1850.12617306852902663No Hit
CGTGGCGGCAAGACCAATCCTGTAGTGAAAGTCGGATTCAGTGACCTCTTCC1850.12617306852902663No Hit
CGTGGCGGCAGCGTTACAGTTTGGGTTTCATTGCTGTGGTATAAGAACAGTG1840.12549105194238325No Hit
CGTGGCGGCGTCTTTCTCCCCGGATAGAAAAGGGGGACCTCAGCATATGATG1840.12549105194238325No Hit
CGTGGCGAGGGATGGACAGTTTAGAGGTATAGTCCTCTGCTGTGTTGGACAG1790.1220809690091663No Hit
CGTGGCGGGGTGTGGTGTCGCGCCCTCTCCCCTTGGGGGCTGGGTGGGCACA1790.1220809690091663No Hit
CGTGGCGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.1220809690091663No Hit
CGTGGCGGGGTCTTGTGAAAGTGAGATAGCCGTGCTTACTGACCTGGGCTGT1790.1220809690091663No Hit
CGTGGCGGGATATAAAGGGAGCCTTAATGGATTTGTTTTCATAATTTAATAT1780.12139895242252291No Hit
CGTGGCGGGCAGGGGCTGAAGAGGGGACTCAGCAGTTAAGAGCACTGGCTGT1780.12139895242252291No Hit
CGTGGCGGGGTCCTTGTGTCTGCCCCGTAAAGATGAGCGGGATCTGAGCCTT1770.12071693583587953No Hit
CGTGGCGGATTTGAAGATTTGTTTGGTTTTAGTGATTTATTGCCTCAAAAAA1760.12003491924923614No Hit
CGTGGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT1750.11935290266259277No Hit
CGTGGCGGCCCGCACGGGTGAACCAGTGGCACAGGTACACCAGCATCGCCGG1740.11867088607594937No Hit
CGTGGCGGGACTCACTGCTATGAAAACATGCAGGACTCCGTTTCCTTTCCTT1740.11867088607594937No Hit
CGTGGCGGGCAATGGGAGAAATCATGAATCGACTTCTGCCTACTGGTCCTTC1730.11798886948930598No Hit
CGTGGCGGGATCTAGCTTCGTGCTCAGACACGTCTCAACAAGACCTTAGAAC1730.11798886948930598No Hit
CGTGGCGGGAAGATCATGATTGCTTAGAGATGTTTTTTATTAAAGATCACCA1730.11798886948930598No Hit
CGTGGCGGGATACATACATGCATACATACATAGGAATAGTACTCAGCCATAA1720.11730685290266259No Hit
CGTGGCGGGAAGTTGGTATCATCTTAAGAAACTGCAAGAGAAAAGCTGGGCT1710.11662483631601919No Hit
CGTGGCGGGGTTAAAGAATAGAACCAAGCCTTGAACTCCTCATCTACCAAGT1710.11662483631601919No Hit
CGTGGCGGGGGTGGTGCATGCCTTTAATCCCAGCACATGGGAGACAGAGGCA1700.11594281972937583No Hit
CGTGGCGGAGGTGCCAGCCGGTATTGCCTTGTATTTCAGGAATGCTCTGGTT1690.11526080314273243No Hit
CGTGGCGGGACCCCTGCACGAGGCAAATCATAGTCAGCTGCTGTGGACAGCC1680.11457878655608904No Hit
CGTGGCGGGGCCTAGAACATATGTAGTATTCAGACAGTCATCAATGAAGGAT1680.11457878655608904No Hit
CGTGGCGGGATGTTCTGTACAGTAGATTTCATTAAGAAAAATTGTGGACAAA1650.11253273679615888No Hit
CGTGGCGGAGATATGGGATGCACATGCACAGGTGCATTGTGGGTAGAGTGCT1650.11253273679615888No Hit
CGTGGCGGGAAGCCAAATCAGAACTGATTGGATGACTTCTGCAAGCCTGAAT1640.1118507202095155No Hit
CGTGGCGGAGCTCGCTCTCAGGAACGCCAGGGCTCCCGAGGGCGGTGCCACA1640.1118507202095155No Hit
CGTGGCGGGAGCTATGGCTGCATTTCCCAAGAAGCAGAACTAATGTCGTCAT1640.1118507202095155No Hit
CGTGGCGGGGTCAAACTGCTCCAGTATTAGTTGCAGGCACTGATGCTTCTGT1630.11116870362287211No Hit
CGTGGCGGGGCTTGGCATATGTGGTCCTCATTCATGCCGCCTGTGGTAATGC1630.11116870362287211No Hit
CGTGGCGGGATGAAACTCAACTGTGACTCTCGATCAGCCCAGCCCATGTGAC1620.11048668703622871No Hit
CGTGGCGGTCATCTACATCGAACGAGTCCAGCGAGAGAAGGCTAATGGCACA1620.11048668703622871No Hit
CGTGGCGGGAGATGAGTTGAAACGGATTGAGAGAGAACTGGCGGAAGCTCAA1620.11048668703622871No Hit
CGTGGCGGGATGTAACGCCCTTGCTGCTTCTGTGTGTGGGTGATTGGATGTG1620.11048668703622871No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.10980467044958532No Hit
CGTGGCGGGCAATGTCTGGCCCTCAAACCCAAACTTTCCTACCAGGCAACCA1610.10980467044958532No Hit
CGTGGCGGGATGAAGTTCACTGTAAAGCTGCTGATAATGAAGGTCTTTCAGA1600.10912265386294195No Hit
CGTGGCGGGACCTCAGAAAAAAATGCTTCATTGAGTATTCATTAAACCACAG1600.10912265386294195No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.10912265386294195No Hit
CGTGGCGGGAGATAAGTTTTTAATTAAAAAGATTGAGCAGTAAAAAGAATTA1580.10775862068965517No Hit
CGTGGCGGGACTTCAGACTTTACTTGTGGTGTGACCATGTTCATTATAATCT1580.10775862068965517No Hit
CGTGGCGGGAGTTATCAGTGGAATACCTGGGGCCTCTGGCTGAGTTCCTTTC1580.10775862068965517No Hit
CGTGGCGGAACTGAGTGAGTTCCAGGACAGCCAGGGCTACACTGAGAAACCT1570.10707660410301179No Hit
CGTGGCGGGGTCAAGGACCCAGGTGGTTCTCTTTCCTCTCTAGGGTCTTTAT1570.10707660410301179No Hit
CGTGGCGGAGTATAAGGATGAAAAAGAAAGCCTTGTTAAGGTTATAAAAGTT1570.10707660410301179No Hit
CGTGGCGGATGAAGAGCTGTACTGGTTTTATAGATCACCCCCAATTGTATGG1560.10639458751636839No Hit
CGTGGCGGGCTGTATGATATATGAAGAAGAGGAGGAGGAGGAGAGAAGAAGA1530.10434853775643824No Hit
CGTGGCGGGGGATGATGCTTCCAGGACAAATCGGCTTCCTCTCCCCCCCCTC1520.10366652116979484No Hit
CGTGGCGGGGTGTGGCAGTTTTTAAGTTATTAGTTTTCAAAATCAGTACTTT1520.10366652116979484No Hit
CGTGGCAGGATAAATGGATTTTGAAATGTCTGACCTCACCTGTTAAGTCCCA1510.10298450458315145No Hit
CGTGGCGGGCTCTGAAAGAACAATCCATGATTGTGTATTTTCAGTGTCCCCT1510.10298450458315145No Hit
CGTGGCGGGATCCTTCTCCTGTCCTGCAACAACATCCATATCCAGCCAGGTG1500.10230248799650808No Hit
CGTGGCGGGGACCAGGTTGTGACAGTTTGTTGTTGGCCAGATCCACCATCTC1500.10230248799650808No Hit
CGTGGCGAGAGAGAGACTCCTGCTGGTATCAGACTCTCAGGATCTGCCAGGA1500.10230248799650808No Hit
CGTGGCGGGGTTCGTGTGGAACCTGGCGCTAGACCATTCGTAGACGACCTGC1490.10162047140986469No Hit
CGTGGCGGGACCTAGTTGAGTGGGGAGGGGGGGGCATCTTCTGACAGCCTGC1490.10162047140986469No Hit
CGTGGCGGGATGCACAAATTTAAACCAAGACATGGCTGTTCAAAGAAAATTA1470.10025643823657791No Hit
CGTGGCGGGATCCCCTTCCCTTTCTCAGATGTGTGTTCAAAACGAAGGATTG1470.10025643823657791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTT351.0114309E-746.00000415
TAGAGCG351.0114309E-746.00000434
GCGGTAG700.046.0000045
ACGCACG351.0114309E-746.00000416
CCGGCTA351.0114309E-746.00000444
GTTTAGC351.0114309E-746.00000420
GACCGGC351.0114309E-746.00000442
GATCCGA351.0114309E-746.0000049
GCAAACG351.0114309E-746.00000446
ATAGATA351.0114309E-746.00000427
ATGCTAG351.0114309E-746.00000420
GTAGGTT351.0114309E-746.00000416
GGATATC850.046.0000048
GCTTGCG351.0114309E-746.00000432
CGGGCTA351.0114309E-746.0000046
AACGCAC351.0114309E-746.00000415
GCGGGAC13800.046.0000045
ATCTTGC351.0114309E-746.00000421
GATATCA351.0114309E-746.0000049
TTGCGTG351.0114309E-746.00000434
Produced by FastQC (version 0.11.5)